P0ABG0 (PGSA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
EC=2.7.8.5

Alternative name(s):
Phosphatidylglycerophosphate synthase
Short name=PGP synthase

Gene names

Name:	pgsA
Ordered Locus Names:	Z3000, ECs2650

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein catalyzes the committed step to the synthesis of the acidic phospholipids By similarity. HAMAP MF_01437
Catalytic activity	CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate. HAMAP MF_01437
Pathway	Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2. HAMAP MF_01437
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01437.
Sequence similarities	Belongs to the CDP-alcohol phosphatidyltransferase class-I family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 182	181	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase HAMAP MF_01437		PRO_0000056774
Regions
	Topological domain	2 – 13	12	Cytoplasmic Potential
	Transmembrane	14 – 38	25	Helical; Potential
	Topological domain	39 – 61	23	Periplasmic Potential
	Transmembrane	62 – 82	21	Helical; Potential
	Topological domain	83 – 87	5	Cytoplasmic Potential
	Transmembrane	88 – 108	21	Helical; Potential
	Topological domain	109 – 146	38	Periplasmic Potential
	Transmembrane	147 – 169	23	Helical; Potential
	Topological domain	170 – 182	13	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P0ABG0 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F8F0502ABE6DC8D5	FASTA	182	20,701
	10 20 30 40 50 60 MQFNIPTLLT LFRVILIPFF VLVFYLPVTW SPFAAALIFC VAAVTDWFDG FLARRWNQST 70 80 90 100 110 120 RFGAFLDPVA DKVLVAIAMV LVTEHYHSWW VTLPAATMIA REIIISALRE WMAELGKRSS 130 140 150 160 170 180 VAVSWIGKVK TTAQMVALAW LLWRPNIWVE YAGIALFFVA AVLTLWSMLQ YLSAARADLL DQ

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG56927.1. BA000007 Genomic DNA. Translation: BAB36073.1.
PIR	B90960. C85808.
RefSeq	NP_288373.1. NC_002655.2. NP_310677.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0ABG0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000026635; EBESCP00000025528; EBESCG00000025688. EBESCT00000058874; EBESCP00000056702; EBESCG00000057922.
GeneID	913922. 961910.
GenomeReviews	Gene locus Z3000 in contig AE005174_GR. Gene locus ECs2650 in contig BA000007_GR.
KEGG	ece:Z3000. ecs:ECs2650.
PATRIC	18354682. VBIEscCol44059_2549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009502.
HOGENOM	HBG686655.
OMA	WSYMAAS.
ProtClustDB	PRK10832.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2650-MONOMER.
Family and domain databases
HAMAP	MF_01437. PgsA. [Tree]
InterPro	IPR000462. CDP-OH_P_trans. IPR023762. PGP_synthase_bac. IPR004570. Phosphatidylglycerol_P_synth. [Graphical view]
KO	K00995.
Pfam	PF01066. CDP-OH_P_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000847. Phos_ph_gly_syn. 1 hit.
TIGRFAMs	TIGR00560. PgsA. 1 hit.
PROSITE	PS00379. CDP_ALCOHOL_P_TRANSF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGSA_ECO57

Accession

Primary (citable) accession number: P0ABG0
Secondary accession number(s): P06978

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program