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Protein

CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase

Gene

pgsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein catalyzes the committed step to the synthesis of the acidic phospholipids.

Catalytic activityi

CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate.

Pathwayi: phosphatidylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA)
  2. Phosphatidylglycerophosphatase C (pgpC), Phosphatidylglycerophosphatase A (pgpA), Phosphatidylglycerophosphatase B (pgpB)
This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:PHOSPHAGLYPSYN-MONOMER.
ECOL316407:JW1897-MONOMER.
MetaCyc:PHOSPHAGLYPSYN-MONOMER.
UniPathwayiUPA00084; UER00503.

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC:2.7.8.5)
Alternative name(s):
Phosphatidylglycerophosphate synthase
Short name:
PGP synthase
Gene namesi
Name:pgsA
Ordered Locus Names:b1912, JW1897
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10706. pgsA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 13CytoplasmicSequence analysisAdd BLAST12
Transmembranei14 – 38HelicalSequence analysisAdd BLAST25
Topological domaini39 – 61PeriplasmicSequence analysisAdd BLAST23
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Topological domaini83 – 87CytoplasmicSequence analysis5
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Topological domaini109 – 146PeriplasmicSequence analysisAdd BLAST38
Transmembranei147 – 169HelicalSequence analysisAdd BLAST23
Topological domaini170 – 182CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi60T → P in pgsA3; lower activity. 1
Mutagenesisi92T → I in pgsA10; lower activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00000567732 – 182CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferaseAdd BLAST181

Proteomic databases

PaxDbiP0ABF8.
PRIDEiP0ABF8.

Interactioni

Protein-protein interaction databases

BioGridi4261587. 230 interactors.
IntActiP0ABF8. 1 interactor.
STRINGi511145.b1912.

Structurei

3D structure databases

ProteinModelPortaliP0ABF8.
SMRiP0ABF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZQ. Bacteria.
COG0558. LUCA.
HOGENOMiHOG000010898.
InParanoidiP0ABF8.
KOiK00995.
OMAiQMVAIPF.
PhylomeDBiP0ABF8.

Family and domain databases

HAMAPiMF_01437. PgsA. 1 hit.
InterProiIPR000462. CDP-OH_P_trans.
IPR023762. PGP_synthase_bac.
IPR004570. Phosphatidylglycerol_P_synth.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000847. Phos_ph_gly_syn. 1 hit.
TIGRFAMsiTIGR00560. pgsA. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFNIPTLLT LFRVILIPFF VLVFYLPVTW SPFAAALIFC VAAVTDWFDG
60 70 80 90 100
FLARRWNQST RFGAFLDPVA DKVLVAIAMV LVTEHYHSWW VTLPAATMIA
110 120 130 140 150
REIIISALRE WMAELGKRSS VAVSWIGKVK TTAQMVALAW LLWRPNIWVE
160 170 180
YAGIALFFVA AVLTLWSMLQ YLSAARADLL DQ
Length:182
Mass (Da):20,701
Last modified:January 23, 2007 - v2
Checksum:iF8F0502ABE6DC8D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12299 Genomic DNA. Translation: AAA98754.1.
U00096 Genomic DNA. Translation: AAC74979.1.
AP009048 Genomic DNA. Translation: BAA15732.1.
PIRiE64954. XNECPG.
RefSeqiNP_416422.1. NC_000913.3.
WP_001160187.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74979; AAC74979; b1912.
BAA15732; BAA15732; BAA15732.
GeneIDi945791.
KEGGiecj:JW1897.
eco:b1912.
PATRICi32119157. VBIEscCol129921_1993.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12299 Genomic DNA. Translation: AAA98754.1.
U00096 Genomic DNA. Translation: AAC74979.1.
AP009048 Genomic DNA. Translation: BAA15732.1.
PIRiE64954. XNECPG.
RefSeqiNP_416422.1. NC_000913.3.
WP_001160187.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABF8.
SMRiP0ABF8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261587. 230 interactors.
IntActiP0ABF8. 1 interactor.
STRINGi511145.b1912.

Proteomic databases

PaxDbiP0ABF8.
PRIDEiP0ABF8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74979; AAC74979; b1912.
BAA15732; BAA15732; BAA15732.
GeneIDi945791.
KEGGiecj:JW1897.
eco:b1912.
PATRICi32119157. VBIEscCol129921_1993.

Organism-specific databases

EchoBASEiEB0700.
EcoGeneiEG10706. pgsA.

Phylogenomic databases

eggNOGiENOG4105BZQ. Bacteria.
COG0558. LUCA.
HOGENOMiHOG000010898.
InParanoidiP0ABF8.
KOiK00995.
OMAiQMVAIPF.
PhylomeDBiP0ABF8.

Enzyme and pathway databases

UniPathwayiUPA00084; UER00503.
BioCyciEcoCyc:PHOSPHAGLYPSYN-MONOMER.
ECOL316407:JW1897-MONOMER.
MetaCyc:PHOSPHAGLYPSYN-MONOMER.

Miscellaneous databases

PROiP0ABF8.

Family and domain databases

HAMAPiMF_01437. PgsA. 1 hit.
InterProiIPR000462. CDP-OH_P_trans.
IPR023762. PGP_synthase_bac.
IPR004570. Phosphatidylglycerol_P_synth.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000847. Phos_ph_gly_syn. 1 hit.
TIGRFAMsiTIGR00560. pgsA. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGSA_ECOLI
AccessioniPrimary (citable) accession number: P0ABF8
Secondary accession number(s): P06978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.