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Protein

CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase

Gene

pgsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein catalyzes the committed step to the synthesis of the acidic phospholipids.

Catalytic activityi

CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate.

Pathwayi: phosphatidylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA)
  2. Phosphatidylglycerophosphatase C (pgpC), Phosphatidylglycerophosphatase A (pgpA), Phosphatidylglycerophosphatase B (pgpB)
This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:PHOSPHAGLYPSYN-MONOMER.
ECOL316407:JW1897-MONOMER.
MetaCyc:PHOSPHAGLYPSYN-MONOMER.
UniPathwayiUPA00084; UER00503.

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC:2.7.8.5)
Alternative name(s):
Phosphatidylglycerophosphate synthase
Short name:
PGP synthase
Gene namesi
Name:pgsA
Ordered Locus Names:b1912, JW1897
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10706. pgsA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1312CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3825HelicalSequence analysisAdd
BLAST
Topological domaini39 – 6123PeriplasmicSequence analysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence analysisAdd
BLAST
Topological domaini83 – 875CytoplasmicSequence analysis
Transmembranei88 – 10821HelicalSequence analysisAdd
BLAST
Topological domaini109 – 14638PeriplasmicSequence analysisAdd
BLAST
Transmembranei147 – 16923HelicalSequence analysisAdd
BLAST
Topological domaini170 – 18213CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi60 – 601T → P in pgsA3; lower activity.
Mutagenesisi92 – 921T → I in pgsA10; lower activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCurated
Chaini2 – 182181CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferasePRO_0000056773Add
BLAST

Proteomic databases

PaxDbiP0ABF8.

Interactioni

Protein-protein interaction databases

BioGridi4261587. 230 interactions.
IntActiP0ABF8. 1 interaction.
STRINGi511145.b1912.

Structurei

3D structure databases

ProteinModelPortaliP0ABF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZQ. Bacteria.
COG0558. LUCA.
HOGENOMiHOG000010898.
InParanoidiP0ABF8.
KOiK00995.
OMAiVQMVAIP.
OrthoDBiEOG6WMJ3D.
PhylomeDBiP0ABF8.

Family and domain databases

HAMAPiMF_01437. PgsA.
InterProiIPR000462. CDP-OH_P_trans.
IPR023762. PGP_synthase_bac.
IPR004570. Phosphatidylglycerol_P_synth.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000847. Phos_ph_gly_syn. 1 hit.
TIGRFAMsiTIGR00560. pgsA. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFNIPTLLT LFRVILIPFF VLVFYLPVTW SPFAAALIFC VAAVTDWFDG
60 70 80 90 100
FLARRWNQST RFGAFLDPVA DKVLVAIAMV LVTEHYHSWW VTLPAATMIA
110 120 130 140 150
REIIISALRE WMAELGKRSS VAVSWIGKVK TTAQMVALAW LLWRPNIWVE
160 170 180
YAGIALFFVA AVLTLWSMLQ YLSAARADLL DQ
Length:182
Mass (Da):20,701
Last modified:January 23, 2007 - v2
Checksum:iF8F0502ABE6DC8D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12299 Genomic DNA. Translation: AAA98754.1.
U00096 Genomic DNA. Translation: AAC74979.1.
AP009048 Genomic DNA. Translation: BAA15732.1.
PIRiE64954. XNECPG.
RefSeqiNP_416422.1. NC_000913.3.
WP_001160187.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74979; AAC74979; b1912.
BAA15732; BAA15732; BAA15732.
GeneIDi945791.
KEGGiecj:JW1897.
eco:b1912.
PATRICi32119157. VBIEscCol129921_1993.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12299 Genomic DNA. Translation: AAA98754.1.
U00096 Genomic DNA. Translation: AAC74979.1.
AP009048 Genomic DNA. Translation: BAA15732.1.
PIRiE64954. XNECPG.
RefSeqiNP_416422.1. NC_000913.3.
WP_001160187.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABF8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261587. 230 interactions.
IntActiP0ABF8. 1 interaction.
STRINGi511145.b1912.

Proteomic databases

PaxDbiP0ABF8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74979; AAC74979; b1912.
BAA15732; BAA15732; BAA15732.
GeneIDi945791.
KEGGiecj:JW1897.
eco:b1912.
PATRICi32119157. VBIEscCol129921_1993.

Organism-specific databases

EchoBASEiEB0700.
EcoGeneiEG10706. pgsA.

Phylogenomic databases

eggNOGiENOG4105BZQ. Bacteria.
COG0558. LUCA.
HOGENOMiHOG000010898.
InParanoidiP0ABF8.
KOiK00995.
OMAiVQMVAIP.
OrthoDBiEOG6WMJ3D.
PhylomeDBiP0ABF8.

Enzyme and pathway databases

UniPathwayiUPA00084; UER00503.
BioCyciEcoCyc:PHOSPHAGLYPSYN-MONOMER.
ECOL316407:JW1897-MONOMER.
MetaCyc:PHOSPHAGLYPSYN-MONOMER.

Miscellaneous databases

PROiP0ABF8.

Family and domain databases

HAMAPiMF_01437. PgsA.
InterProiIPR000462. CDP-OH_P_trans.
IPR023762. PGP_synthase_bac.
IPR004570. Phosphatidylglycerol_P_synth.
[Graphical view]
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF000847. Phos_ph_gly_syn. 1 hit.
TIGRFAMsiTIGR00560. pgsA. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of the gene locus encoding the phosphatidylglycerophosphate synthase of Escherichia coli."
    Gopalakrishnan A.S., Chen Y.-C., Temkin M., Dowhan W.
    J. Biol. Chem. 261:1329-1338(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Primary structures of the wild-type and mutant alleles encoding the phosphatidylglycerophosphate synthase of Escherichia coli."
    Usui M., Sembongi H., Matsuzaki H., Matsumoto K., Shibuya I.
    J. Bacteriol. 176:3389-3392(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION, MUTANTS PGSA3 AND PGSA10.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Phosphatidylglycerophosphate synthase from Escherichia coli."
    Dowhan W.
    Methods Enzymol. 209:313-321(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  8. "Two-dimensional 1H-NMR of transmembrane peptides from Escherichia coli phosphatidylglycerophosphate synthase in micelles."
    Morein S., Trouard T.P., Hauksson J.B., Arvidson G., Lindblom G.
    Eur. J. Biochem. 241:489-497(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 6-25 AND 149-176.

Entry informationi

Entry nameiPGSA_ECOLI
AccessioniPrimary (citable) accession number: P0ABF8
Secondary accession number(s): P06978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.