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Protein

CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase

Gene

pgsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein catalyzes the committed step to the synthesis of the acidic phospholipids.

Catalytic activityi

CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate.

Pathwayi: phosphatidylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA)
  2. Phosphatidylglycerophosphatase C (pgpC), Phosphatidylglycerophosphatase A (pgpA), Phosphatidylglycerophosphatase B (pgpB)
This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:PHOSPHAGLYPSYN-MONOMER
MetaCyc:PHOSPHAGLYPSYN-MONOMER
UniPathwayiUPA00084; UER00503

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC:2.7.8.5)
Alternative name(s):
Phosphatidylglycerophosphate synthase
Short name:
PGP synthase
Gene namesi
Name:pgsA
Ordered Locus Names:b1912, JW1897
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10706 pgsA

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 13CytoplasmicSequence analysisAdd BLAST12
Transmembranei14 – 38HelicalSequence analysisAdd BLAST25
Topological domaini39 – 61PeriplasmicSequence analysisAdd BLAST23
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Topological domaini83 – 87CytoplasmicSequence analysis5
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Topological domaini109 – 146PeriplasmicSequence analysisAdd BLAST38
Transmembranei147 – 169HelicalSequence analysisAdd BLAST23
Topological domaini170 – 182CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi60T → P in pgsA3; lower activity. 1
Mutagenesisi92T → I in pgsA10; lower activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00000567732 – 182CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferaseAdd BLAST181

Proteomic databases

PaxDbiP0ABF8
PRIDEiP0ABF8

Interactioni

Protein-protein interaction databases

BioGridi4261587, 267 interactors
IntActiP0ABF8, 1 interactor
STRINGi316385.ECDH10B_2053

Structurei

3D structure databases

ProteinModelPortaliP0ABF8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZQ Bacteria
COG0558 LUCA
HOGENOMiHOG000010898
InParanoidiP0ABF8
KOiK00995
OMAiIDYTIKY
PhylomeDBiP0ABF8

Family and domain databases

HAMAPiMF_01437 PgsA, 1 hit
InterProiView protein in InterPro
IPR000462 CDP-OH_P_trans
IPR023762 PGP_synthase_bac
IPR004570 Phosphatidylglycerol_P_synth
PfamiView protein in Pfam
PF01066 CDP-OH_P_transf, 1 hit
PIRSFiPIRSF000847 Phos_ph_gly_syn, 1 hit
TIGRFAMsiTIGR00560 pgsA, 1 hit
PROSITEiView protein in PROSITE
PS00379 CDP_ALCOHOL_P_TRANSF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFNIPTLLT LFRVILIPFF VLVFYLPVTW SPFAAALIFC VAAVTDWFDG
60 70 80 90 100
FLARRWNQST RFGAFLDPVA DKVLVAIAMV LVTEHYHSWW VTLPAATMIA
110 120 130 140 150
REIIISALRE WMAELGKRSS VAVSWIGKVK TTAQMVALAW LLWRPNIWVE
160 170 180
YAGIALFFVA AVLTLWSMLQ YLSAARADLL DQ
Length:182
Mass (Da):20,701
Last modified:January 23, 2007 - v2
Checksum:iF8F0502ABE6DC8D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12299 Genomic DNA Translation: AAA98754.1
U00096 Genomic DNA Translation: AAC74979.1
AP009048 Genomic DNA Translation: BAA15732.1
PIRiE64954 XNECPG
RefSeqiNP_416422.1, NC_000913.3
WP_001160187.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74979; AAC74979; b1912
BAA15732; BAA15732; BAA15732
GeneIDi945791
KEGGiecj:JW1897
eco:b1912
PATRICifig|1411691.4.peg.338

Similar proteinsi

Entry informationi

Entry nameiPGSA_ECOLI
AccessioniPrimary (citable) accession number: P0ABF8
Secondary accession number(s): P06978
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health