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P0ABF3

- PCNB_ECO57

UniProt

P0ABF3 - PCNB_ECO57

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Protein

Poly(A) polymerase I

Gene
pcnB, Z0154, ECs0147
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control By similarity.UniRule annotation

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801 By similarity
Active sitei82 – 821 By similarity
Active sitei162 – 1621 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. polynucleotide adenylyltransferase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA polyadenylation Source: InterPro
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing, Transcription

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-144-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase I (EC:2.7.7.19)
Short name:
PAP I
Gene namesi
Name:pcnB
Ordered Locus Names:Z0154, ECs0147
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Poly(A) polymerase IUniRule annotationPRO_0000139091Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi155864.Z0154.

Structurei

3D structure databases

ProteinModelPortaliP0ABF3.
SMRiP0ABF3. Positions 28-438.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0617.
HOGENOMiHOG000256527.
KOiK00970.
OMAiRFMAKLD.
OrthoDBiEOG6PZX7Q.

Family and domain databases

HAMAPiMF_00957. PolyA_pol.
InterProiIPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01942. pcnB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABF3-1 [UniParc]FASTAAdd to Basket

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MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK    50
VMYRLNKAGY EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR 100
LVGRRFRLAH VMFGPEIIEV ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI 150
FGSIEEDAQR RDFTINSLYY SVADFTVRDY VGGMKDLKDG VIRLIGNPET 200
RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND IPPARLFEES 250
LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK 300
NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN 350
DVLDEACRSL AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA 400
YDLLALRAEV ERNAELQRLV KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR 450
TRRPRKRAPR REGTA 465
Length:465
Mass (Da):53,871
Last modified:September 19, 2006 - v2
Checksum:i0FAE30F2F95FB76E
GO

Sequence cautioni

The sequence AAG54447.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence BAB33570.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG54447.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB33570.1. Different initiation.
RefSeqiNP_285839.2. NC_002655.2.
NP_308174.2. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG54447; AAG54447; Z0154.
BAB33570; BAB33570; BAB33570.
GeneIDi913763.
956853.
KEGGiece:Z0154.
ecs:ECs0147.
PATRICi18349234. VBIEscCol44059_0147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG54447.1 . Different initiation.
BA000007 Genomic DNA. Translation: BAB33570.1 . Different initiation.
RefSeqi NP_285839.2. NC_002655.2.
NP_308174.2. NC_002695.1.

3D structure databases

ProteinModelPortali P0ABF3.
SMRi P0ABF3. Positions 28-438.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z0154.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG54447 ; AAG54447 ; Z0154 .
BAB33570 ; BAB33570 ; BAB33570 .
GeneIDi 913763.
956853.
KEGGi ece:Z0154.
ecs:ECs0147.
PATRICi 18349234. VBIEscCol44059_0147.

Phylogenomic databases

eggNOGi COG0617.
HOGENOMi HOG000256527.
KOi K00970.
OMAi RFMAKLD.
OrthoDBi EOG6PZX7Q.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-144-MONOMER.

Family and domain databases

HAMAPi MF_00957. PolyA_pol.
InterProi IPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view ]
Pfami PF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01942. pcnB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiPCNB_ECO57
AccessioniPrimary (citable) accession number: P0ABF3
Secondary accession number(s): P13685, P78050, Q8X910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: September 19, 2006
Last modified: June 11, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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