P0ABF3 (PCNB_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase
Short name=PAP
EC=2.7.7.19

Alternative name(s):
Plasmid copy number protein

Gene names

Name:	pcnB
Ordered Locus Names:	Z0154, ECs0147

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polymerase that creates the 3' poly(A) tail found in some mRNAs. Seems to be involved in plasmid copy number control By similarity.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Monomer. Interacts with CsdA, RhlE and SrmB By similarity.
Miscellaneous	Uses an AUU start codon By similarity.
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.
Sequence caution	The sequence AAG54447.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAB33570.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Plasmid copy control Transcription mRNA processing
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA polyadenylation Inferred from electronic annotation. Source: InterPro plasmid maintenance Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 10	10	Potential		PRO_0000411164
	Chain	11 – 465	455	Poly(A) polymerase		PRO_0000139091
Sites
	Active site	82	1	By similarity
	Active site	84	1	By similarity
	Active site	164	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0ABF3 [UniParc]. Last modified September 19, 2006. Version 2. Checksum: 0FAE30F2F95FB76E	FASTA	465	53,871
	10 20 30 40 50 60 MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK VMYRLNKAGY 70 80 90 100 110 120 EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR LVGRRFRLAH VMFGPEIIEV 130 140 150 160 170 180 ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI FGSIEEDAQR RDFTINSLYY SVADFTVRDY 190 200 210 220 230 240 VGGMKDLKDG VIRLIGNPET RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND 250 260 270 280 290 300 IPPARLFEES LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK 310 320 330 340 350 360 NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN DVLDEACRSL 370 380 390 400 410 420 AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA YDLLALRAEV ERNAELQRLV 430 440 450 460 KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR TRRPRKRAPR REGTA

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG54447.1. Different initiation. BA000007 Genomic DNA. Translation: BAB33570.1. Different initiation.
RefSeq	NP_285839.2. NC_002655.2. NP_308174.2. NC_002695.1.
3D structure databases
ProteinModelPortal	P0ABF3.
SMR	P0ABF3. Positions 28-438.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z0154.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG54447; AAG54447; Z0154. BAB33570; BAB33570; BAB33570.
GeneID	913763. 956853.
KEGG	ece:Z0154. ecs:ECs0147.
PATRIC	18349234. VBIEscCol44059_0147.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0617.
HOGENOM	HOG000256527.
KO	K00970.
OMA	RFMAKLD.
ProtClustDB	PRK11623.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-144-MONOMER.
Family and domain databases
InterPro	IPR026007. PcnB/CCA-adding. IPR002646. PolA_pol_head_dom. IPR010206. PolA_pol_PcnB. IPR025866. PolyA_pol_arg_C_dom. [Graphical view]
PANTHER	PTHR13734:SF5. PTHR13734:SF5. 1 hit.
Pfam	PF01743. PolyA_pol. 1 hit. PF12626. PolyA_pol_arg_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01942. pcnB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PCNB_ECO57

Accession

Primary (citable) accession number: P0ABF3
Secondary accession number(s): P13685, P78050, Q8X910

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	September 19, 2006
Last modified:	May 1, 2013
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program