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Protein

Poly(A) polymerase I

Gene

pcnB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control.UniRule annotation

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei80UniRule annotation1
Active sitei82UniRule annotation1
Active sitei162UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase
Biological processmRNA processing, Transcription
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase IUniRule annotation (EC:2.7.7.19UniRule annotation)
Short name:
PAP IUniRule annotation
Gene namesi
Name:pcnBUniRule annotation
Ordered Locus Names:Z0154, ECs0147
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001390911 – 465Poly(A) polymerase IAdd BLAST465

Proteomic databases

PRIDEiP0ABF3

Interactioni

Protein-protein interaction databases

STRINGi155864.Z0154

Structurei

3D structure databases

ProteinModelPortaliP0ABF3
SMRiP0ABF3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ0 Bacteria
COG0617 LUCA
HOGENOMiHOG000256527
KOiK00970
OMAiFMAKLDM

Family and domain databases

CDDicd05398 NT_ClassII-CCAase, 1 hit
HAMAPiMF_00957 PolyA_pol, 1 hit
InterProiView protein in InterPro
IPR002646 PolA_pol_head_dom
IPR010206 PolA_pol_I
IPR025866 PolyA_pol_arg_C_dom
IPR032828 PolyA_RNA-bd
PfamiView protein in Pfam
PF01743 PolyA_pol, 1 hit
PF12626 PolyA_pol_arg_C, 1 hit
PF12627 PolyA_pol_RNAbd, 1 hit
TIGRFAMsiTIGR01942 pcnB, 1 hit

Sequencei

Sequence statusi: Complete.

P0ABF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK
60 70 80 90 100
VMYRLNKAGY EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR
110 120 130 140 150
LVGRRFRLAH VMFGPEIIEV ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI
160 170 180 190 200
FGSIEEDAQR RDFTINSLYY SVADFTVRDY VGGMKDLKDG VIRLIGNPET
210 220 230 240 250
RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND IPPARLFEES
260 270 280 290 300
LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK
310 320 330 340 350
NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN
360 370 380 390 400
DVLDEACRSL AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA
410 420 430 440 450
YDLLALRAEV ERNAELQRLV KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR
460
TRRPRKRAPR REGTA
Length:465
Mass (Da):53,871
Last modified:September 19, 2006 - v2
Checksum:i0FAE30F2F95FB76E
GO

Sequence cautioni

The sequence AAG54447 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB33570 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG54447.1 Different initiation.
BA000007 Genomic DNA Translation: BAB33570.1 Different initiation.
RefSeqiNP_308174.3, NC_002695.1

Genome annotation databases

EnsemblBacteriaiAAG54447; AAG54447; Z0154
BAB33570; BAB33570; BAB33570
GeneIDi913763
KEGGiece:Z0154
ecs:ECs0147
PATRICifig|386585.9.peg.246

Entry informationi

Entry nameiPCNB_ECO57
AccessioniPrimary (citable) accession number: P0ABF3
Secondary accession number(s): P13685, P78050, Q8X910
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: September 19, 2006
Last modified: April 25, 2018
This is version 73 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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