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P0ABF3 (PCNB_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) polymerase I

Short name=PAP I
EC=2.7.7.19
Gene names
Name:pcnB
Ordered Locus Names:Z0154, ECs0147
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control By similarity. HAMAP-Rule MF_00957

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00957

Sequence similarities

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.

Sequence caution

The sequence AAG54447.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAB33570.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 465465Poly(A) polymerase I HAMAP-Rule MF_00957
PRO_0000139091

Sites

Active site801 By similarity
Active site821 By similarity
Active site1621 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0ABF3 [UniParc].

Last modified September 19, 2006. Version 2.
Checksum: 0FAE30F2F95FB76E

FASTA46553,871
        10         20         30         40         50         60 
MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK VMYRLNKAGY 

        70         80         90        100        110        120 
EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR LVGRRFRLAH VMFGPEIIEV 

       130        140        150        160        170        180 
ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI FGSIEEDAQR RDFTINSLYY SVADFTVRDY 

       190        200        210        220        230        240 
VGGMKDLKDG VIRLIGNPET RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND 

       250        260        270        280        290        300 
IPPARLFEES LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK 

       310        320        330        340        350        360 
NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN DVLDEACRSL 

       370        380        390        400        410        420 
AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA YDLLALRAEV ERNAELQRLV 

       430        440        450        460 
KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR TRRPRKRAPR REGTA 

« Hide

References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG54447.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB33570.1. Different initiation.
RefSeqNP_285839.2. NC_002655.2.
NP_308174.2. NC_002695.1.

3D structure databases

ProteinModelPortalP0ABF3.
SMRP0ABF3. Positions 28-438.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING155864.Z0154.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG54447; AAG54447; Z0154.
BAB33570; BAB33570; BAB33570.
GeneID913763.
956853.
KEGGece:Z0154.
ecs:ECs0147.
PATRIC18349234. VBIEscCol44059_0147.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0617.
HOGENOMHOG000256527.
KOK00970.
OMARFMAKLD.
OrthoDBEOG6PZX7Q.

Enzyme and pathway databases

BioCycECOL386585:GJFA-144-MONOMER.

Family and domain databases

HAMAPMF_00957. PolyA_pol.
InterProIPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PfamPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR01942. pcnB. 1 hit.
ProtoNetSearch...

Entry information

Entry namePCNB_ECO57
AccessionPrimary (citable) accession number: P0ABF3
Secondary accession number(s): P13685, P78050, Q8X910
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: September 19, 2006
Last modified: June 11, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families