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Protein

Poly(A) polymerase I

Gene

pcnB

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control.UniRule annotation

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801UniRule annotation
Active sitei82 – 821UniRule annotation
Active sitei162 – 1621UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. polynucleotide adenylyltransferase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA polyadenylation Source: InterPro
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing, Transcription

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase IUniRule annotation (EC:2.7.7.19UniRule annotation)
Short name:
PAP IUniRule annotation
Gene namesi
Name:pcnBUniRule annotation
Ordered Locus Names:c0176
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001410 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Poly(A) polymerase IPRO_0000139092Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi199310.c0176.

Structurei

3D structure databases

ProteinModelPortaliP0ABF2.
SMRiP0ABF2. Positions 28-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000256527.
KOiK00970.
OMAiRFMAKLD.
OrthoDBiEOG6PZX7Q.

Family and domain databases

HAMAPiMF_00957. PolyA_pol.
InterProiIPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01942. pcnB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK
60 70 80 90 100
VMYRLNKAGY EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR
110 120 130 140 150
LVGRRFRLAH VMFGPEIIEV ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI
160 170 180 190 200
FGSIEEDAQR RDFTINSLYY SVADFTVRDY VGGMKDLKDG VIRLIGNPET
210 220 230 240 250
RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND IPPARLFEES
260 270 280 290 300
LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK
310 320 330 340 350
NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN
360 370 380 390 400
DVLDEACRSL AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA
410 420 430 440 450
YDLLALRAEV ERNAELQRLV KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR
460
TRRPRKRAPR REGTA
Length:465
Mass (Da):53,871
Last modified:September 19, 2006 - v2
Checksum:i0FAE30F2F95FB76E
GO

Sequence cautioni

The sequence AAN78670.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN78670.1. Different initiation.
RefSeqiNP_752126.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN78670; AAN78670; c0176.
GeneIDi1037253.
KEGGiecc:c0176.
PATRICi18278422. VBIEscCol75197_0166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA. Translation: AAN78670.1. Different initiation.
RefSeqiNP_752126.1. NC_004431.1.

3D structure databases

ProteinModelPortaliP0ABF2.
SMRiP0ABF2. Positions 28-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi199310.c0176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN78670; AAN78670; c0176.
GeneIDi1037253.
KEGGiecc:c0176.
PATRICi18278422. VBIEscCol75197_0166.

Phylogenomic databases

HOGENOMiHOG000256527.
KOiK00970.
OMAiRFMAKLD.
OrthoDBiEOG6PZX7Q.

Family and domain databases

HAMAPiMF_00957. PolyA_pol.
InterProiIPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01942. pcnB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiPCNB_ECOL6
AccessioniPrimary (citable) accession number: P0ABF2
Secondary accession number(s): P13685, P78050, Q8X910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: September 19, 2006
Last modified: January 7, 2015
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.