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P0ABF1 (PCNB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) polymerase I

Short name=PAP I
EC=2.7.7.19
Alternative name(s):
Plasmid copy number protein
Gene names
Name:pcnB
Ordered Locus Names:b0143, JW5808
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Rho-independent transcription terminators may serve as polyadenylation signals. Seems to be involved in plasmid copy number control. Ref.5 Ref.8 Ref.11

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1). Ref.5

Subunit structure

Monomer. Interacts with CsdA, RhlE and SrmB. Ref.7

Subcellular location

Cytoplasm. Cell inner membrane Ref.10.

Induction

Transcription regulation of pcnB is a complex process that involves several factors such as Sigma-70 (rpoD), Sigma-S (rpoS), ppGpp and DksA. Ref.12

Miscellaneous

Uses the non-canonical initiation codon AUU, which limits pcnB expression (Ref.9).

Sequence similarities

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.

Sequence caution

The sequence AAA24303.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAB24139.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1010 HAMAP-Rule MF_00957
PRO_0000411163
Chain11 – 465455Poly(A) polymerase I HAMAP-Rule MF_00957
PRO_0000139090

Sites

Active site801 By similarity
Active site821 By similarity
Active site1621 By similarity

Experimental info

Sequence conflict931R → A in AAA24303. Ref.1
Sequence conflict3151A → T in AAA24303. Ref.1
Sequence conflict3321K → N in AAA24303. Ref.1
Sequence conflict437 – 4382ML → IV in AAA24303. Ref.1
Sequence conflict449 – 46517RRTRR…REGTA → QSYSSSTQTRTTS in AAA24303. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0ABF1 [UniParc].

Last modified September 19, 2006. Version 2.
Checksum: 0FAE30F2F95FB76E

FASTA46553,871
        10         20         30         40         50         60 
MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK VMYRLNKAGY 

        70         80         90        100        110        120 
EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR LVGRRFRLAH VMFGPEIIEV 

       130        140        150        160        170        180 
ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI FGSIEEDAQR RDFTINSLYY SVADFTVRDY 

       190        200        210        220        230        240 
VGGMKDLKDG VIRLIGNPET RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND 

       250        260        270        280        290        300 
IPPARLFEES LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK 

       310        320        330        340        350        360 
NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN DVLDEACRSL 

       370        380        390        400        410        420 
AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA YDLLALRAEV ERNAELQRLV 

       430        440        450        460 
KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR TRRPRKRAPR REGTA 

« Hide

References

« Hide 'large scale' references
[1]"Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control."
Liu J., Parkinson J.S.
J. Bacteriol. 171:1254-1261(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RP437.
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
Fujita N., Mori H., Yura T., Ishihama A.
Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Identification of the gene for an Escherichia coli poly(A) polymerase."
Cao G.-J., Sarkar N.
Proc. Natl. Acad. Sci. U.S.A. 89:10380-10384(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64, PROTEIN SEQUENCE OF 11-25, FUNCTION, CATALYTIC ACTIVITY.
Strain: K12.
[6]"A possible role for the pcnB gene product of Escherichia coli in modulating RNA: RNA interactions."
Masters M., March J.B., Oliver I.R., Collins J.F.
Mol. Gen. Genet. 220:341-344(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO CCA.
[7]"Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation."
Raynal L.C., Carpousis A.J.
Mol. Microbiol. 32:765-775(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS, INTERACTION WITH CSDA; RHLE AND SRMB.
[8]"Analysis of the function of Escherichia coli poly(A) polymerase I in RNA metabolism."
Mohanty B.K., Kushner S.R.
Mol. Microbiol. 34:1094-1108(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: MG1693.
[9]"Expression of the Escherichia coli pcnB gene is translationally limited using an inefficient start codon: a second chromosomal example of translation initiated at AUU."
Binns N., Masters M.
Mol. Microbiol. 44:1287-1298(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF INITIATION SITE.
[10]"Localization of Escherichia coli poly(A) polymerase I in cellular membrane."
Jasiecki J., Wegrzyn G.
Biochem. Biophys. Res. Commun. 329:598-602(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: K12 / MG1655 / ATCC 47076.
[11]"The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells."
Mohanty B.K., Kushner S.R.
Nucleic Acids Res. 34:5695-5704(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: MG1693.
[12]"Transcription regulation of the Escherichia coli pcnB gene coding for poly(A) polymerase I: roles of ppGpp, DksA and sigma factors."
Nadratowska-Wesolowska B., Slominska-Wojewodzka M., Lyzen R., Wegrzyn A., Szalewska-Palasz A., Wegrzyn G.
Mol. Genet. Genomics 284:289-305(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20574 Genomic DNA. Translation: AAA24303.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73254.2.
AP009048 Genomic DNA. Translation: BAB96720.2.
S48039 Genomic DNA. Translation: AAB24139.1. Different initiation.
PIRG64737.
RefSeqNP_414685.4. NC_000913.3.
YP_488446.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0ABF1.
SMRP0ABF1. Positions 28-438.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47859N.
IntActP0ABF1. 8 interactions.
STRING511145.b0143.

Proteomic databases

PaxDbP0ABF1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73254; AAC73254; b0143.
BAB96720; BAB96720; BAB96720.
GeneID12934035.
947318.
KEGGecj:Y75_p0140.
eco:b0143.
PATRIC32115393. VBIEscCol129921_0148.

Organism-specific databases

EchoBASEEB0684.
EcoGeneEG10690. pcnB.

Phylogenomic databases

eggNOGCOG0617.
HOGENOMHOG000256527.
KOK00970.
OMARFMAKLD.
OrthoDBEOG6PZX7Q.
PhylomeDBP0ABF1.
ProtClustDBPRK11623.

Enzyme and pathway databases

BioCycEcoCyc:EG10690-MONOMER.
ECOL316407:JW5808-MONOMER.
MetaCyc:EG10690-MONOMER.

Gene expression databases

GenevestigatorP0ABF1.

Family and domain databases

HAMAPMF_00957. PolyA_pol.
InterProIPR026007. PcnB/CCA-adding.
IPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PANTHERPTHR13734:SF5. PTHR13734:SF5. 1 hit.
PfamPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR01942. pcnB. 1 hit.
ProtoNetSearch...

Other

PROP0ABF1.

Entry information

Entry namePCNB_ECOLI
AccessionPrimary (citable) accession number: P0ABF1
Secondary accession number(s): P13685, P78050, Q8X910
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: September 19, 2006
Last modified: April 16, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene