Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Poly(A) polymerase I

Gene

pcnB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Rho-independent transcription terminators may serve as polyadenylation signals. Seems to be involved in plasmid copy number control.UniRule annotation3 Publications

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801UniRule annotation
Active sitei82 – 821UniRule annotation
Active sitei162 – 1621UniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • polynucleotide adenylyltransferase activity Source: EcoliWiki
  • RNA binding Source: EcoliWiki

GO - Biological processi

  • mRNA polyadenylation Source: EcoliWiki
  • mRNA processing Source: EcoliWiki
  • plasmid maintenance Source: EcoliWiki
  • RNA modification Source: EcoliWiki
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing, Plasmid copy control, Transcription

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10690-MONOMER.
ECOL316407:JW5808-MONOMER.
MetaCyc:EG10690-MONOMER.
BRENDAi2.7.7.19. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase IUniRule annotation (EC:2.7.7.19UniRule annotation)
Short name:
PAP IUniRule annotation
Alternative name(s):
Plasmid copy number protein
Gene namesi
Name:pcnBUniRule annotation
Ordered Locus Names:b0143, JW5808
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10690. pcnB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 10101 PublicationPRO_0000411163
Chaini11 – 465455Poly(A) polymerase IPRO_0000139090Add
BLAST

Proteomic databases

PaxDbiP0ABF1.

Expressioni

Inductioni

Transcription regulation of pcnB is a complex process that involves several factors such as Sigma-70 (rpoD), Sigma-S (rpoS), ppGpp and DksA.1 Publication

Interactioni

Subunit structurei

Monomer. Interacts with CsdA, RhlE and SrmB.1 Publication

Protein-protein interaction databases

DIPiDIP-47859N.
IntActiP0ABF1. 8 interactions.
STRINGi511145.b0143.

Structurei

3D structure databases

ProteinModelPortaliP0ABF1.
SMRiP0ABF1. Positions 28-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0617.
HOGENOMiHOG000256527.
InParanoidiP0ABF1.
KOiK00970.
OMAiRFMAKLD.
OrthoDBiEOG6PZX7Q.
PhylomeDBiP0ABF1.

Family and domain databases

HAMAPiMF_00957. PolyA_pol.
InterProiIPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01942. pcnB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK
60 70 80 90 100
VMYRLNKAGY EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR
110 120 130 140 150
LVGRRFRLAH VMFGPEIIEV ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI
160 170 180 190 200
FGSIEEDAQR RDFTINSLYY SVADFTVRDY VGGMKDLKDG VIRLIGNPET
210 220 230 240 250
RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND IPPARLFEES
260 270 280 290 300
LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK
310 320 330 340 350
NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN
360 370 380 390 400
DVLDEACRSL AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA
410 420 430 440 450
YDLLALRAEV ERNAELQRLV KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR
460
TRRPRKRAPR REGTA
Length:465
Mass (Da):53,871
Last modified:September 19, 2006 - v2
Checksum:i0FAE30F2F95FB76E
GO

Sequence cautioni

The sequence AAA24303.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAB24139.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931R → A in AAA24303 (PubMed:2537812).Curated
Sequence conflicti315 – 3151A → T in AAA24303 (PubMed:2537812).Curated
Sequence conflicti332 – 3321K → N in AAA24303 (PubMed:2537812).Curated
Sequence conflicti437 – 4382ML → IV in AAA24303 (PubMed:2537812).Curated
Sequence conflicti449 – 46517RRTRR…REGTA → QSYSSSTQTRTTS in AAA24303 (PubMed:2537812).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20574 Genomic DNA. Translation: AAA24303.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73254.2.
AP009048 Genomic DNA. Translation: BAB96720.2.
S48039 Genomic DNA. Translation: AAB24139.1. Different initiation.
PIRiG64737.
RefSeqiNP_414685.4. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73254; AAC73254; b0143.
BAB96720; BAB96720; BAB96720.
GeneIDi947318.
KEGGiecj:Y75_p0140.
eco:b0143.
PATRICi32115393. VBIEscCol129921_0148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20574 Genomic DNA. Translation: AAA24303.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73254.2.
AP009048 Genomic DNA. Translation: BAB96720.2.
S48039 Genomic DNA. Translation: AAB24139.1. Different initiation.
PIRiG64737.
RefSeqiNP_414685.4. NC_000913.3.

3D structure databases

ProteinModelPortaliP0ABF1.
SMRiP0ABF1. Positions 28-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47859N.
IntActiP0ABF1. 8 interactions.
STRINGi511145.b0143.

Proteomic databases

PaxDbiP0ABF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73254; AAC73254; b0143.
BAB96720; BAB96720; BAB96720.
GeneIDi947318.
KEGGiecj:Y75_p0140.
eco:b0143.
PATRICi32115393. VBIEscCol129921_0148.

Organism-specific databases

EchoBASEiEB0684.
EcoGeneiEG10690. pcnB.

Phylogenomic databases

eggNOGiCOG0617.
HOGENOMiHOG000256527.
InParanoidiP0ABF1.
KOiK00970.
OMAiRFMAKLD.
OrthoDBiEOG6PZX7Q.
PhylomeDBiP0ABF1.

Enzyme and pathway databases

BioCyciEcoCyc:EG10690-MONOMER.
ECOL316407:JW5808-MONOMER.
MetaCyc:EG10690-MONOMER.
BRENDAi2.7.7.19. 2026.

Miscellaneous databases

PROiP0ABF1.

Family and domain databases

HAMAPiMF_00957. PolyA_pol.
InterProiIPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01942. pcnB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control."
    Liu J., Parkinson J.S.
    J. Bacteriol. 171:1254-1261(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RP437.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification of the gene for an Escherichia coli poly(A) polymerase."
    Cao G.-J., Sarkar N.
    Proc. Natl. Acad. Sci. U.S.A. 89:10380-10384(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64, PROTEIN SEQUENCE OF 11-25, FUNCTION, CATALYTIC ACTIVITY.
    Strain: K12.
  6. "A possible role for the pcnB gene product of Escherichia coli in modulating RNA: RNA interactions."
    Masters M., March J.B., Oliver I.R., Collins J.F.
    Mol. Gen. Genet. 220:341-344(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO CCA.
  7. "Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation."
    Raynal L.C., Carpousis A.J.
    Mol. Microbiol. 32:765-775(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS, INTERACTION WITH CSDA; RHLE AND SRMB.
  8. "Analysis of the function of Escherichia coli poly(A) polymerase I in RNA metabolism."
    Mohanty B.K., Kushner S.R.
    Mol. Microbiol. 34:1094-1108(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: MG1693.
  9. "Expression of the Escherichia coli pcnB gene is translationally limited using an inefficient start codon: a second chromosomal example of translation initiated at AUU."
    Binns N., Masters M.
    Mol. Microbiol. 44:1287-1298(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INITIATION SITE.
  10. "Localization of Escherichia coli poly(A) polymerase I in cellular membrane."
    Jasiecki J., Wegrzyn G.
    Biochem. Biophys. Res. Commun. 329:598-602(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  11. "The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells."
    Mohanty B.K., Kushner S.R.
    Nucleic Acids Res. 34:5695-5704(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: MG1693.
  12. "Transcription regulation of the Escherichia coli pcnB gene coding for poly(A) polymerase I: roles of ppGpp, DksA and sigma factors."
    Nadratowska-Wesolowska B., Slominska-Wojewodzka M., Lyzen R., Wegrzyn A., Szalewska-Palasz A., Wegrzyn G.
    Mol. Genet. Genomics 284:289-305(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPCNB_ECOLI
AccessioniPrimary (citable) accession number: P0ABF1
Secondary accession number(s): P13685, P78050, Q8X910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: September 19, 2006
Last modified: June 24, 2015
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Uses the non-canonical initiation codon AUU, which limits pcnB expression.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.