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P0ABF1

- PCNB_ECOLI

UniProt

P0ABF1 - PCNB_ECOLI

Protein

Poly(A) polymerase I

Gene

pcnB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (19 Sep 2006)
      Previous versions | rss
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    Functioni

    Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Rho-independent transcription terminators may serve as polyadenylation signals. Seems to be involved in plasmid copy number control.3 PublicationsUniRule annotation

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).1 PublicationUniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei80 – 801UniRule annotation
    Active sitei82 – 821UniRule annotation
    Active sitei162 – 1621UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. polynucleotide adenylyltransferase activity Source: EcoliWiki
    3. RNA binding Source: EcoliWiki

    GO - Biological processi

    1. mRNA polyadenylation Source: EcoliWiki
    2. mRNA processing Source: EcoliWiki
    3. plasmid maintenance Source: EcoliWiki
    4. RNA modification Source: EcoliWiki
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing, Plasmid copy control, Transcription

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10690-MONOMER.
    ECOL316407:JW5808-MONOMER.
    MetaCyc:EG10690-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase IUniRule annotation (EC:2.7.7.19UniRule annotation)
    Short name:
    PAP IUniRule annotation
    Alternative name(s):
    Plasmid copy number protein
    Gene namesi
    Name:pcnBUniRule annotation
    Ordered Locus Names:b0143, JW5808
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10690. pcnB.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell inner membrane 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: EcoliWiki
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 10101 PublicationPRO_0000411163
    Chaini11 – 465455Poly(A) polymerase IPRO_0000139090Add
    BLAST

    Proteomic databases

    PaxDbiP0ABF1.

    Expressioni

    Inductioni

    Transcription regulation of pcnB is a complex process that involves several factors such as Sigma-70 (rpoD), Sigma-S (rpoS), ppGpp and DksA.1 Publication

    Gene expression databases

    GenevestigatoriP0ABF1.

    Interactioni

    Subunit structurei

    Monomer. Interacts with CsdA, RhlE and SrmB.1 Publication

    Protein-protein interaction databases

    DIPiDIP-47859N.
    IntActiP0ABF1. 8 interactions.
    STRINGi511145.b0143.

    Structurei

    3D structure databases

    ProteinModelPortaliP0ABF1.
    SMRiP0ABF1. Positions 28-438.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0617.
    HOGENOMiHOG000256527.
    KOiK00970.
    OMAiRFMAKLD.
    OrthoDBiEOG6PZX7Q.
    PhylomeDBiP0ABF1.

    Family and domain databases

    HAMAPiMF_00957. PolyA_pol.
    InterProiIPR002646. PolA_pol_head_dom.
    IPR010206. PolA_pol_I.
    IPR025866. PolyA_pol_arg_C_dom.
    [Graphical view]
    PfamiPF01743. PolyA_pol. 1 hit.
    PF12626. PolyA_pol_arg_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01942. pcnB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0ABF1-1 [UniParc]FASTAAdd to Basket

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    MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK    50
    VMYRLNKAGY EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR 100
    LVGRRFRLAH VMFGPEIIEV ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI 150
    FGSIEEDAQR RDFTINSLYY SVADFTVRDY VGGMKDLKDG VIRLIGNPET 200
    RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND IPPARLFEES 250
    LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK 300
    NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN 350
    DVLDEACRSL AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA 400
    YDLLALRAEV ERNAELQRLV KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR 450
    TRRPRKRAPR REGTA 465
    Length:465
    Mass (Da):53,871
    Last modified:September 19, 2006 - v2
    Checksum:i0FAE30F2F95FB76E
    GO

    Sequence cautioni

    The sequence AAA24303.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAB24139.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti93 – 931R → A in AAA24303. (PubMed:2537812)Curated
    Sequence conflicti315 – 3151A → T in AAA24303. (PubMed:2537812)Curated
    Sequence conflicti332 – 3321K → N in AAA24303. (PubMed:2537812)Curated
    Sequence conflicti437 – 4382ML → IV in AAA24303. (PubMed:2537812)Curated
    Sequence conflicti449 – 46517RRTRR…REGTA → QSYSSSTQTRTTS in AAA24303. (PubMed:2537812)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20574 Genomic DNA. Translation: AAA24303.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC73254.2.
    AP009048 Genomic DNA. Translation: BAB96720.2.
    S48039 Genomic DNA. Translation: AAB24139.1. Different initiation.
    PIRiG64737.
    RefSeqiNP_414685.4. NC_000913.3.
    YP_488446.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73254; AAC73254; b0143.
    BAB96720; BAB96720; BAB96720.
    GeneIDi12934035.
    947318.
    KEGGiecj:Y75_p0140.
    eco:b0143.
    PATRICi32115393. VBIEscCol129921_0148.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20574 Genomic DNA. Translation: AAA24303.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC73254.2 .
    AP009048 Genomic DNA. Translation: BAB96720.2 .
    S48039 Genomic DNA. Translation: AAB24139.1 . Different initiation.
    PIRi G64737.
    RefSeqi NP_414685.4. NC_000913.3.
    YP_488446.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0ABF1.
    SMRi P0ABF1. Positions 28-438.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47859N.
    IntActi P0ABF1. 8 interactions.
    STRINGi 511145.b0143.

    Proteomic databases

    PaxDbi P0ABF1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73254 ; AAC73254 ; b0143 .
    BAB96720 ; BAB96720 ; BAB96720 .
    GeneIDi 12934035.
    947318.
    KEGGi ecj:Y75_p0140.
    eco:b0143.
    PATRICi 32115393. VBIEscCol129921_0148.

    Organism-specific databases

    EchoBASEi EB0684.
    EcoGenei EG10690. pcnB.

    Phylogenomic databases

    eggNOGi COG0617.
    HOGENOMi HOG000256527.
    KOi K00970.
    OMAi RFMAKLD.
    OrthoDBi EOG6PZX7Q.
    PhylomeDBi P0ABF1.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10690-MONOMER.
    ECOL316407:JW5808-MONOMER.
    MetaCyc:EG10690-MONOMER.

    Miscellaneous databases

    PROi P0ABF1.

    Gene expression databases

    Genevestigatori P0ABF1.

    Family and domain databases

    HAMAPi MF_00957. PolyA_pol.
    InterProi IPR002646. PolA_pol_head_dom.
    IPR010206. PolA_pol_I.
    IPR025866. PolyA_pol_arg_C_dom.
    [Graphical view ]
    Pfami PF01743. PolyA_pol. 1 hit.
    PF12626. PolyA_pol_arg_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01942. pcnB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control."
      Liu J., Parkinson J.S.
      J. Bacteriol. 171:1254-1261(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RP437.
    2. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
      Fujita N., Mori H., Yura T., Ishihama A.
      Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Identification of the gene for an Escherichia coli poly(A) polymerase."
      Cao G.-J., Sarkar N.
      Proc. Natl. Acad. Sci. U.S.A. 89:10380-10384(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64, PROTEIN SEQUENCE OF 11-25, FUNCTION, CATALYTIC ACTIVITY.
      Strain: K12.
    6. "A possible role for the pcnB gene product of Escherichia coli in modulating RNA: RNA interactions."
      Masters M., March J.B., Oliver I.R., Collins J.F.
      Mol. Gen. Genet. 220:341-344(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO CCA.
    7. "Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation."
      Raynal L.C., Carpousis A.J.
      Mol. Microbiol. 32:765-775(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS, INTERACTION WITH CSDA; RHLE AND SRMB.
    8. "Analysis of the function of Escherichia coli poly(A) polymerase I in RNA metabolism."
      Mohanty B.K., Kushner S.R.
      Mol. Microbiol. 34:1094-1108(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: MG1693.
    9. "Expression of the Escherichia coli pcnB gene is translationally limited using an inefficient start codon: a second chromosomal example of translation initiated at AUU."
      Binns N., Masters M.
      Mol. Microbiol. 44:1287-1298(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF INITIATION SITE.
    10. "Localization of Escherichia coli poly(A) polymerase I in cellular membrane."
      Jasiecki J., Wegrzyn G.
      Biochem. Biophys. Res. Commun. 329:598-602(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Strain: K12 / MG1655 / ATCC 47076.
    11. "The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells."
      Mohanty B.K., Kushner S.R.
      Nucleic Acids Res. 34:5695-5704(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: MG1693.
    12. "Transcription regulation of the Escherichia coli pcnB gene coding for poly(A) polymerase I: roles of ppGpp, DksA and sigma factors."
      Nadratowska-Wesolowska B., Slominska-Wojewodzka M., Lyzen R., Wegrzyn A., Szalewska-Palasz A., Wegrzyn G.
      Mol. Genet. Genomics 284:289-305(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiPCNB_ECOLI
    AccessioniPrimary (citable) accession number: P0ABF1
    Secondary accession number(s): P13685, P78050, Q8X910
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: September 19, 2006
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Uses the non-canonical initiation codon AUU, which limits pcnB expression.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3