SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0ABF1

- PCNB_ECOLI

UniProt

P0ABF1 - PCNB_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Poly(A) polymerase I
Gene
pcnB, b0143, JW5808
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control. Rho-independent transcription terminators may serve as polyadenylation signals. Seems to be involved in plasmid copy number control.3 Publications

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801 By similarity
Active sitei82 – 821 By similarity
Active sitei162 – 1621 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. RNA binding Source: EcoliWiki
  3. polynucleotide adenylyltransferase activity Source: EcoliWiki
Complete GO annotation...

GO - Biological processi

  1. RNA modification Source: EcoliWiki
  2. mRNA polyadenylation Source: EcoliWiki
  3. mRNA processing Source: EcoliWiki
  4. plasmid maintenance Source: EcoliWiki
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing, Plasmid copy control, Transcription

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10690-MONOMER.
ECOL316407:JW5808-MONOMER.
MetaCyc:EG10690-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase I (EC:2.7.7.19)
Short name:
PAP I
Alternative name(s):
Plasmid copy number protein
Gene namesi
Name:pcnB
Ordered Locus Names:b0143, JW5808
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10690. pcnB.

Subcellular locationi

Cytoplasm. Cell inner membrane 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: EcoliWiki
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1010UniRule annotation
PRO_0000411163
Chaini11 – 465455Poly(A) polymerase IUniRule annotation
PRO_0000139090Add
BLAST

Proteomic databases

PaxDbiP0ABF1.

Expressioni

Inductioni

Transcription regulation of pcnB is a complex process that involves several factors such as Sigma-70 (rpoD), Sigma-S (rpoS), ppGpp and DksA.1 Publication

Gene expression databases

GenevestigatoriP0ABF1.

Interactioni

Subunit structurei

Monomer. Interacts with CsdA, RhlE and SrmB.1 Publication

Protein-protein interaction databases

DIPiDIP-47859N.
IntActiP0ABF1. 8 interactions.
STRINGi511145.b0143.

Structurei

3D structure databases

ProteinModelPortaliP0ABF1.
SMRiP0ABF1. Positions 28-438.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0617.
HOGENOMiHOG000256527.
KOiK00970.
OMAiRFMAKLD.
OrthoDBiEOG6PZX7Q.
PhylomeDBiP0ABF1.

Family and domain databases

HAMAPiMF_00957. PolyA_pol.
InterProiIPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01942. pcnB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABF1-1 [UniParc]FASTAAdd to Basket

« Hide

MFTRVANFCR KVLSREESEA EQAVARPQVT VIPREQHAIS RKDISENALK    50
VMYRLNKAGY EAWLVGGGVR DLLLGKKPKD FDVTTNATPE QVRKLFRNCR 100
LVGRRFRLAH VMFGPEIIEV ATFRGHHEGN VSDRTTSQRG QNGMLLRDNI 150
FGSIEEDAQR RDFTINSLYY SVADFTVRDY VGGMKDLKDG VIRLIGNPET 200
RYREDPVRML RAVRFAAKLG MRISPETAEP IPRLATLLND IPPARLFEES 250
LKLLQAGYGY ETYKLLCEYH LFQPLFPTIT RYFTENGDSP MERIIEQVLK 300
NTDTRIHNDM RVNPAFLFAA MFWYPLLETA QKIAQESGLT YHDAFALAMN 350
DVLDEACRSL AIPKRLTTLT RDIWQLQLRM SRRQGKRAWK LLEHPKFRAA 400
YDLLALRAEV ERNAELQRLV KWWGEFQVSA PPDQKGMLNE LDEEPSPRRR 450
TRRPRKRAPR REGTA 465
Length:465
Mass (Da):53,871
Last modified:September 19, 2006 - v2
Checksum:i0FAE30F2F95FB76E
GO

Sequence cautioni

The sequence AAA24303.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAB24139.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931R → A in AAA24303. 1 Publication
Sequence conflicti315 – 3151A → T in AAA24303. 1 Publication
Sequence conflicti332 – 3321K → N in AAA24303. 1 Publication
Sequence conflicti437 – 4382ML → IV in AAA24303. 1 Publication
Sequence conflicti449 – 46517RRTRR…REGTA → QSYSSSTQTRTTS in AAA24303. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20574 Genomic DNA. Translation: AAA24303.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73254.2.
AP009048 Genomic DNA. Translation: BAB96720.2.
S48039 Genomic DNA. Translation: AAB24139.1. Different initiation.
PIRiG64737.
RefSeqiNP_414685.4. NC_000913.3.
YP_488446.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73254; AAC73254; b0143.
BAB96720; BAB96720; BAB96720.
GeneIDi12934035.
947318.
KEGGiecj:Y75_p0140.
eco:b0143.
PATRICi32115393. VBIEscCol129921_0148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20574 Genomic DNA. Translation: AAA24303.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC73254.2 .
AP009048 Genomic DNA. Translation: BAB96720.2 .
S48039 Genomic DNA. Translation: AAB24139.1 . Different initiation.
PIRi G64737.
RefSeqi NP_414685.4. NC_000913.3.
YP_488446.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0ABF1.
SMRi P0ABF1. Positions 28-438.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-47859N.
IntActi P0ABF1. 8 interactions.
STRINGi 511145.b0143.

Proteomic databases

PaxDbi P0ABF1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73254 ; AAC73254 ; b0143 .
BAB96720 ; BAB96720 ; BAB96720 .
GeneIDi 12934035.
947318.
KEGGi ecj:Y75_p0140.
eco:b0143.
PATRICi 32115393. VBIEscCol129921_0148.

Organism-specific databases

EchoBASEi EB0684.
EcoGenei EG10690. pcnB.

Phylogenomic databases

eggNOGi COG0617.
HOGENOMi HOG000256527.
KOi K00970.
OMAi RFMAKLD.
OrthoDBi EOG6PZX7Q.
PhylomeDBi P0ABF1.

Enzyme and pathway databases

BioCyci EcoCyc:EG10690-MONOMER.
ECOL316407:JW5808-MONOMER.
MetaCyc:EG10690-MONOMER.

Miscellaneous databases

PROi P0ABF1.

Gene expression databases

Genevestigatori P0ABF1.

Family and domain databases

HAMAPi MF_00957. PolyA_pol.
InterProi IPR002646. PolA_pol_head_dom.
IPR010206. PolA_pol_I.
IPR025866. PolyA_pol_arg_C_dom.
[Graphical view ]
Pfami PF01743. PolyA_pol. 1 hit.
PF12626. PolyA_pol_arg_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01942. pcnB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control."
    Liu J., Parkinson J.S.
    J. Bacteriol. 171:1254-1261(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RP437.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification of the gene for an Escherichia coli poly(A) polymerase."
    Cao G.-J., Sarkar N.
    Proc. Natl. Acad. Sci. U.S.A. 89:10380-10384(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64, PROTEIN SEQUENCE OF 11-25, FUNCTION, CATALYTIC ACTIVITY.
    Strain: K12.
  6. "A possible role for the pcnB gene product of Escherichia coli in modulating RNA: RNA interactions."
    Masters M., March J.B., Oliver I.R., Collins J.F.
    Mol. Gen. Genet. 220:341-344(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO CCA.
  7. "Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation."
    Raynal L.C., Carpousis A.J.
    Mol. Microbiol. 32:765-775(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS, INTERACTION WITH CSDA; RHLE AND SRMB.
  8. "Analysis of the function of Escherichia coli poly(A) polymerase I in RNA metabolism."
    Mohanty B.K., Kushner S.R.
    Mol. Microbiol. 34:1094-1108(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: MG1693.
  9. "Expression of the Escherichia coli pcnB gene is translationally limited using an inefficient start codon: a second chromosomal example of translation initiated at AUU."
    Binns N., Masters M.
    Mol. Microbiol. 44:1287-1298(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF INITIATION SITE.
  10. "Localization of Escherichia coli poly(A) polymerase I in cellular membrane."
    Jasiecki J., Wegrzyn G.
    Biochem. Biophys. Res. Commun. 329:598-602(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  11. "The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells."
    Mohanty B.K., Kushner S.R.
    Nucleic Acids Res. 34:5695-5704(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: MG1693.
  12. "Transcription regulation of the Escherichia coli pcnB gene coding for poly(A) polymerase I: roles of ppGpp, DksA and sigma factors."
    Nadratowska-Wesolowska B., Slominska-Wojewodzka M., Lyzen R., Wegrzyn A., Szalewska-Palasz A., Wegrzyn G.
    Mol. Genet. Genomics 284:289-305(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPCNB_ECOLI
AccessioniPrimary (citable) accession number: P0ABF1
Secondary accession number(s): P13685, P78050, Q8X910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: September 19, 2006
Last modified: June 11, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Uses the non-canonical initiation codon AUU, which limits pcnB expression (1 Publication).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi