P0ABF0 (CYNT_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbonic anhydrase 1
EC=4.2.1.1

Alternative name(s):
Carbonate dehydratase 1

Gene names

Name:	cynT
Ordered Locus Names:	Z0435, ECs0392

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to catalyze the hydration of carbon dioxide and thus prevent depletion of cellular bicarbonate By similarity.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Oligomer By similarity.
Sequence similarities	Belongs to the beta-class carbonic anhydrase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbon utilization Inferred from electronic annotation. Source: InterPro
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Carbonic anhydrase 1		PRO_0000077460
Sites
	Metal binding	39	1	Zinc By similarity
	Metal binding	41	1	Zinc By similarity
	Metal binding	98	1	Zinc By similarity
	Metal binding	101	1	Zinc By similarity
Experimental info
	Sequence conflict	59	1	V → G in BAB33815. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0ABF0 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 5EB41F125EC4D731	FASTA	219	23,764
	10 20 30 40 50 60 MKEIIDGFLK FQREAFPKRE ALFKQLATQQ SPRTLFISCS DSRLVPELVT QREPGDLFVI 70 80 90 100 110 120 RNAGNIVPSY GPEPGGVSAS VEYAVAALRV SDIVICGHSN CGAMTAIASC QCMDHMPAVS 130 140 150 160 170 180 HWLRYADSAR VVNEARPHSD LPSKAAAMVR ENVIAQLANL QTHPSVRLAL EEGRIALHGW 190 200 210 VYDIESGSIA AFDGATRQFV PLAANPRVCA IPLRQPTAA

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG54688.1. BA000007 Genomic DNA. Translation: BAB33815.1.
PIR	D85528. H90677.
RefSeq	NP_286080.1. NC_002655.2. NP_308419.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0ABF0.
SMR	P0ABF0. Positions 1-202.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000027255; EBESCP00000026148; EBESCG00000026307. EBESCT00000058004; EBESCP00000055832; EBESCG00000057052.
GeneID	914494. 957265.
GenomeReviews	Gene locus Z0435 in contig AE005174_GR. Gene locus ECs0392 in contig BA000007_GR.
KEGG	ece:Z0435. ecs:ECs0392.
PATRIC	18349731. VBIEscCol44059_0386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009995.
HOGENOM	HBG711150.
OMA	WHYVIED.
ProtClustDB	CLSK866979.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0392-MONOMER.
Family and domain databases
InterPro	IPR001765. Carbonic_anhydrase. IPR015892. Carbonic_anhydrase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1050.10. CO_anhd_prok_pln. 1 hit.
KO	K01673.
PANTHER	PTHR11002. Carbonic_anhydrase. 1 hit.
Pfam	PF00484. Pro_CA. 1 hit. [Graphical view]
SMART	SM00947. Pro_CA. 1 hit. [Graphical view]
SUPFAM	SSF53056. Prok_plnt_COanhd. 1 hit.
PROSITE	PS00704. PROK_CO2_ANHYDRASE_1. 1 hit. PS00705. PROK_CO2_ANHYDRASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYNT_ECO57

Accession

Primary (citable) accession number: P0ABF0
Secondary accession number(s): P17582, P78278

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 25, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program