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Protein

Carbonic anhydrase 1

Gene

cynT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to catalyze the hydration of carbon dioxide and thus prevent depletion of cellular bicarbonate.1 Publication

Catalytic activityi

H2CO3 = CO2 + H2O.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39ZincBy similarity1
Metal bindingi41ZincBy similarity1
Metal bindingi98ZincBy similarity1
Metal bindingi101ZincBy similarity1

GO - Molecular functioni

  • carbonate dehydratase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • carbon utilization Source: InterPro
  • cyanate catabolic process Source: EcoCyc

Keywordsi

Molecular functionLyase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:CARBODEHYDRAT-MONOMER
MetaCyc:CARBODEHYDRAT-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Carbonic anhydrase 1 (EC:4.2.1.11 Publication)
Alternative name(s):
Carbonate dehydratase 1
Gene namesi
Name:cynT1 Publication
Ordered Locus Names:b0339, JW0330
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10176 cynT

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000774591 – 219Carbonic anhydrase 1Add BLAST219

Proteomic databases

PaxDbiP0ABE9
PRIDEiP0ABE9

Expressioni

Inductioni

By cyanate.1 Publication

Interactioni

Subunit structurei

Oligomer.1 Publication

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259815, 4 interactors
DIPiDIP-47965N
IntActiP0ABE9, 1 interactor
STRINGi316407.85674481

Structurei

3D structure databases

ProteinModelPortaliP0ABE9
SMRiP0ABE9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107B2H Bacteria
COG0288 LUCA
HOGENOMiHOG000125182
InParanoidiP0ABE9
KOiK01673
OMAiWHYIIET
PhylomeDBiP0ABE9

Family and domain databases

Gene3Di3.40.1050.10, 1 hit
InterProiView protein in InterPro
IPR001765 Carbonic_anhydrase
IPR015892 Carbonic_anhydrase_CS
IPR036874 Carbonic_anhydrase_sf
PfamiView protein in Pfam
PF00484 Pro_CA, 1 hit
SMARTiView protein in SMART
SM00947 Pro_CA, 1 hit
SUPFAMiSSF53056 SSF53056, 1 hit
PROSITEiView protein in PROSITE
PS00704 PROK_CO2_ANHYDRASE_1, 1 hit
PS00705 PROK_CO2_ANHYDRASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0ABE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEIIDGFLK FQREAFPKRE ALFKQLATQQ SPRTLFISCS DSRLVPELVT
60 70 80 90 100
QREPGDLFVI RNAGNIVPSY GPEPGGVSAS VEYAVAALRV SDIVICGHSN
110 120 130 140 150
CGAMTAIASC QCMDHMPAVS HWLRYADSAR VVNEARPHSD LPSKAAAMVR
160 170 180 190 200
ENVIAQLANL QTHPSVRLAL EEGRIALHGW VYDIESGSIA AFDGATRQFV
210
PLAANPRVCA IPLRQPTAA
Length:219
Mass (Da):23,764
Last modified:October 25, 2005 - v1
Checksum:i5EB41F125EC4D731
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174 – 176RIA → GS in AAA23625 (PubMed:3049588).Curated3
Sequence conflicti212P → R in AAA23625 (PubMed:3049588).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23219 Genomic DNA Translation: AAA23625.1
U73857 Genomic DNA Translation: AAB18063.1
U00096 Genomic DNA Translation: AAC73442.1
AP009048 Genomic DNA Translation: BAE76121.1
PIRiC64761 QRECTC
RefSeqiNP_414873.1, NC_000913.3
WP_000658652.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73442; AAC73442; b0339
BAE76121; BAE76121; BAE76121
GeneIDi946548
KEGGiecj:JW0330
eco:b0339
PATRICifig|1411691.4.peg.1938

Similar proteinsi

Entry informationi

Entry nameiCYNT_ECOLI
AccessioniPrimary (citable) accession number: P0ABE9
Secondary accession number(s): P17582, P78278, Q2MC85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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