true1986-07-212024-03-27139C562_ECOLXCloning and expression of the gene encoding the soluble cytochrome b562 of Escherichia coli.Nikkila H.Gennis R.B.Sligar S.G.doi:10.1111/j.1432-1033.1991.tb16377.x1991Eur. J. Biochem.202309-313NUCLEOTIDE SEQUENCE [GENOMIC DNA]SUBCELLULAR LOCATIONBPCR cloning, sequence analysis and expression of the cybC genes encoding soluble cytochrome b-562 from Escherichia coli B strain OP7 and K strain NM522.Trower M.K.doi:10.1016/0005-2728(93)90223-31993Biochim. Biophys. Acta1143109-111NUCLEOTIDE SEQUENCE [GENOMIC DNA]B / OP7K / NM522The amino acid sequence of cytochrome b562 of Escherichia coli.Itagaki E.Hager L.P.doi:10.1016/0006-291x(68)90130-71968Biochem. Biophys. Res. Commun.321013-1019PROTEIN SEQUENCE OF 23-128The structure of cytochrome b562 from Escherichia coli at 2.5-A resolution.Mathews F.S.Bethge P.H.Czerwinski E.W.doi:10.1016/s0021-9258(17)37829-81979J. Biol. Chem.2541699-1706X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)SUBUNITImprovement of the 2.5-A resolution model of cytochrome b562 by redetermining the primary structure and using molecular graphics.Lederer F.Glatigny A.Bethge P.H.Bellamy H.D.Mathews F.S.doi:10.1016/0022-2836(81)90185-61981J. Mol. Biol.148427-448X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS)SEQUENCE REVISIONRefined structure of cytochrome b562 from Escherichia coli at 1.4-A resolution.Hamada K.Bethge P.H.Mathews F.S.doi:10.1006/jmbi.1995.01921995J. Mol. Biol.247947-962X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS)A multigeneration analysis of cytochrome b562 redox variants: evolutionary strategies for modulating redox potential revealed using a library approach.Springs S.L.Bass S.E.Bowman G.Nodelman I.Schutt C.E.McLendon G.L.doi:10.1021/bi012066s2002Biochemistry414321-4328X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF F83I/F87Y/R128L VARIANTThe solution structure of oxidized Escherichia coli cytochrome b562.Arnesano F.Banci L.Bertini I.Faraone-Mennella J.Rosato A.Barker P.D.Fersht A.R.doi:10.1021/bi982785f1999Biochemistry388657-8670STRUCTURE BY NMR(15)N backbone dynamics of ferricytochrome b(562): comparison with the reduced protein and the R98C variant.Assfalg M.Banci L.Bertini I.Ciofi-Baffoni S.Barker P.D.doi:10.1021/bi01013002001Biochemistry4012761-12771STRUCTURE BY NMRDifferent initiation.CBEC62A=23-1282.70B/D=23-1281.81A=23-128A=23-128A=23-1281.40A/B=23-1282.25A/B/C/D=23-1282.90A/B/C/D=23-1281.87A/B/C/D=23-1281.75A/B/C/D=23-1281.72A/B/C/D=23-1282.04A=23-1282.40A/B/C/D/E/F/G/H/I/J/K/L=23-1283.00A/B=23-1282.35A/B/C/D/E/F/G/H=23-1282.00A/B/C/D=23-1282.10A/B=23-1282.20A/B=23-1282.05A/B/C/D=23-1282.35A/B/C/D/E/F/G/H=23-1282.30A=23-1282.60A/B/C=23-1282.50A/B/C/D=23-1281.90A/B/C/D=23-1282.10A/B/C/D/E/F/G/H=23-1282.01A/B/C/D/E/F=23-1283.10A/B/C/D=23-1282.80A/B/C/D=23-1282.00A/B/C/D=23-1282.30A/B/C/D=23-1282.75A/B/C=23-1283.01A/B=23-1281.80A=23-1282.80A=23-1282.70A=23-1282.70A=23-1282.12A/B=23-1281.22A/B/C/D=23-1282.30A/B=23-1282.45A/B=23-1273.30A=23-1281.80A=23-1282.80A=23-1282.62A=23-1283.20A=23-1282.80A/B=23-1282.50A=23-1283.10A=23-1282.61A=23-1282.60A=23-1283.28A/B=23-1272.70A/B=23-1272.50A/B/C/D=23-1282.80A=23-1282.90A=23-1283.00A=23-64A=73-1272.90A=23-64A=73-1272.90A=23-64A=78-1272.80A=23-1281.85A/B=23-1282.46A/B=23-1283.00A/B=23-1283.00A/B=23-1281.72A=23-1281.90A=23-1282.00A=23-1282.20A=23-1282.10A=23-1282.80A=27-1282.50A=23-1282.50A=23-1281.90A=23-1281.90A=23-1282.40A/B/C/D=23-1282.10A/B/C/D=23-1283.20A/B=23-1273.30A/B=23-1272.00A=23-1282.10A=23-1282.80A=23-1273.30A=22-1272.80A=23-1273.60A=23-1274.00A=23-1272.14A=23-1271.95A=23-1271.70A=23-1271.87A=23-1272.60A=23-1283.10A=23-1272.00A=23-1281.90A=23-1282.10A=23-1282.00A=23-1283.00A/D=23-1282.90A=23-1283.20A/B=23-1273.50A=27-1282.80A/B=23-1282.80B=23-1282.90A/B=23-1283.20A=27-1282.35A=23-1281.96A=24-1282.14A=24-1283.20A=23-1282.65A=23-1281.98A/B/C/D=23-1281.56A=23-1281.70A=23-1281.37A=23-1282.20A/B/C/D/E/F/G/H=23-1281.20A=23-1283.00A/B=23-62A/B=88-1282.90A/B=23-62A/B=88-1281.81A/C/E/G=23-1282.90A/B=23-62A/B=85-1272.51A=23-1283.10A/B=23-1283.00A=23-1282.70A=23-1282.90B=23-1282.20B=23-1281.87B=23-1283.50A=23-1274.50R=23-1273.75A=23-1273.90A=23-1272.22A/B/C/D=23-1282.80A/B/C/D=23-1282.90A/B=24-1223.10A=23-1232.92A=23-1233.10A=23-1233.19A=23-1281.90A/D=23-1281.80A/B=23-1281.90A/B/C/D=23-1282.75A/C=23-1281.33A/B=23-1281.72A/C=23-1282.25A/B=23-1281.10A/B=23-1281.49A/B=23-1281.83A/D=23-1281.96B=23-1281.96A/C/E/G=23-1281.96A/C/E/G=23-1281.69A/C/E/G=23-1282.70A=23-1272.00A=23-1272.50A=23-1283.00A=23-1283.50A/B=24-1273.20C=23-62C=89-1282.25A=23-1283.30A=23-1281.80A=23-1281.80A=23-1282.00A=23-1282.80A=23-1283.03A=23-1283.21A=23-1272.80A/B=23-1283.20A/B=23-1283.10A/B=23-1283.30A/B=23-1284.20A=23-1272.90A=24-1222.79A=24-1222.70A=24-1221.40A/B/C/D=23-1281.85A/B/C/D=23-1281.50A=23-1282.40A/B/C/D=23-1282.60A/B/C/D/E/F/G/H/I/J/K/L=23-1281.85A=23-1282.70A=22-1272.53A/B=22-1272.43A=23-1282.43A=23-1282.70A=23-1282.73A=23-1282.65A=23-1273.71A/D=23-1233.87A/D=23-1233.40A/B/C=23-1283.40A/B/C=23-1283.36A=23-1272.00A=23-1273.70R=23-1273.34R=22-1271.76A/B/C=23-1281.70A/B/C=23-1282.00A/B/C=23-1282.00A/B/C=23-1281.80A/B/C=23-1282.30A/B/C=23-1282.50A/B/C=23-1282.19A=23-1282.00A/B/C=23-1282.50A=23-1281.92A=23-1272.13A=23-1273.12A=23-1283.90R=23-1283.00A=23-1283.40A=23-1283.00R=23-1282.70R=23-1283.10A=24-62A=89-1283.00R=23-1283.00R=23-1283.10R=23-1282.90R=23-1282.90R=23-1283.40R=23-1273.80A/B=23-1233.00R=3-1272.90R=3-1272.70R=3-1273.10R=3-1272.60B=23-1282.94A/B=23-1282.90R=3-1273.20R=3-1273.00A/B=23-1282.80R=23-1273.00A/B/C/D/E=23-1273.60A/B/C/D/E=23-1272.70A/B/C/D/E=23-1271.70A/C=23-1281.70A/C=23-1281.78A/B=23-1281.89A/B=23-1281.40A/C=23-1281.26A/C=23-1281.64A/C=23-1281.52A/C=23-1281.91A/C=23-1281.99A/C=23-1281.27A/B=23-1283.40C/D=23-1271.80A/C=23-1281.93A/C=23-1282.78AAA=23-1282.25A=23-1282.60A=23-1282.79A=23-1281.80A=23-1282.20A/B/C/D=23-1281.70A/B/C/D=23-1281.60A/B=23-1282.20A/B/C/D/E/F=23-1282.54R=23-1282.58R=23-1282.60R=23-1282.90R=23-1273.70C=22-1272.00A/C/D/G=23-1282.50R=23-1273.40A=23-1282.70A/B/C/D=23-1283.30A=25-1273.80A=25-1273.30A=23-62A=88-1282.90A=23-62A=88-1283.57Q/R=23-1273.71R=23-1274.21Q/R=23-1273.78R=23-1273.16R=23-1273.20R=23-1272.80R=23-1272.90R=23-1273.20R=23-1272.60A=23-65A=88-1272.60A=23-65A=88-1272.45A=23-65A=88-1272.50A=23-65A=88-1273.72R=23-1272.90R=23-1273.10R=23-1272.80R=23-1273.00R=23-1273.10R=23-1273.30A=23-1273.40A/B/C/D/E/F/G/H/I/J/K/L=23-1282.20A/B/C/D/E/F/G/H/I/J/K/L=23-1282.10X/Y=24-1283.10R=23-1273.20R=23-1273.20R=23-1273.30R=23-1273.20R=23-1272.70B=23-1272.99R=23-1273.26R=23-1273.10R=23-1272.80R=23-1273.10R=23-1273.10R=23-1273.20R=23-1272.90R=23-1273.30E=23-1272.20A/B=23-1283.00A/B=23-1272.70A=23-1282.70A=23-1273.45A=23-1283.50A=23-1282.11A=23-1282.10A=23-1282.80A=23-1272.05A=23-1271.69A/B/C/D=23-1283.60A=25-1273.33C=25-1273.23C=25-1273.04A/B/C/D/E=23-1273.98A/B=23-1271.89A/B=23-1283.18A/B/C/D/E=23-1271.70A=23-1282.40A/C=23-1282.95A/B/C/D/E=23-1271.65A=23-1281.55A=23-1284.12A=23-1274.06B/C=23-1274.16E=23-1273.07A/B/C/D/E=23-1273.17A/B/C/D/E=23-1273.48A/B/C/D/E=23-1272.50A=23-1283.10A/B/C/D/E=23-1273.15A/B/C/D/E=23-1273.15A/B/C/D/E=23-1273.15A/B/C/D/E=23-1273.92A/B/C/D/E/F/G=23-1274.20A=23-1274.13B/C=23-1274.32E=23-1272.00A=23-1272.30A=23-1283.30R=23-1283.57A=23-1273.54A=23-1273.72A=23-1273.00F=24-1283.01R=23-1272.98R=23-1272.94R=23-1273.60R=23-1272.97R=23-1282.95R=23-1283.00R=23-1283.08R=30-1273.14R=22-1273.22R=30-1273.04R=30-1233.51R=23-1273.19R=23-1272.85R=23-1272.97R=23-1273.06R=23-1273.11R=23-1273.07R=23-1273.21R=23-1273.00R=23-1272.70R=23-1273.10R=23-1272.80E/F=23-1273.20F=23-65F=77-1283.20A=23-65A=78-1283.10R=23-1283.22R=23-1283.24R=23-1283.52R=23-1283.23R=23-1282.84R=23-1283.00R=23-1283.30A=24-1273.40R=23-1283.00R=23-1273.20R=23-1272.40A=23-1232.94R=24-1273.00R=23-12726Hybrid Between B and C Type Hemes (Protoporphyrin Ixcontaining Fe)N-1,10-phenanthrolin-5-ylacetamide2 sequenced antibodiesBacteriaCytochrome c/b562Cyt_b562Cyt_c/b562Cytochrom_B562Cytochrome_b562CytochromesSoluble cytochrome b562Cytochrome b-562cybCElectron-transport protein of unknown function.Binds 1 heme b (iron(II)-protoporphyrin IX) group per molecule.E(0) is about +180 mV.Monomer.Belongs to the cytochrome b562 family.In strains K / NM522 and K12 / MG1655 the cybC gene has a 26 bp deletion and lacks the first 7 amino acids. It is not translated in those strains.122Soluble cytochrome b5621178023128Disordered6082Basic and acidic residues66axial binding residue29axial binding residue124254145626365687071737577781021061141171272005-10-251true140613e21294ba2d2a82f8ec9e70ef8650a05MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYRtruetruetruetrue