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Protein

DNA-binding transcriptional regulator BolA

Gene

bolA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator that plays an important role in general stress response. Has many effects on cell morphology, cell growth and cell division. Acts by regulating the transcription of many genes, including dacA (PBP-5), dacC (PBP-6), ampC and mreB. Probably involved in the coordination of genes that adapt the cell physiology in order to enhance cell adaptation and survival under stress conditions. Essential for normal cell morphology in stationary phase and under conditions of starvation (PubMed:10361282, PubMed:12354237, PubMed:19111750, PubMed:21464593, PubMed:25691594). Also regulates a complex network of genes encoding proteins related to biofilm development, and negatively modulates flagellar biosynthesis and swimming capacity. Could be a motile/adhesive transcriptional switch, specifically involved in the transition between the planktonic and the attachment stage of biofilm formation (PubMed:25691594). Overexpression produces round cell shape, impairs cell growth rate and induces biofilm development (PubMed:305364, PubMed:15345459, PubMed:21464593).7 Publications

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10125-MONOMER.
ECOL316407:JW5060-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding transcriptional regulator BolACurated
Gene namesi
Name:bolA1 Publication
Ordered Locus Names:b0435, JW5060
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10125. bolA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 105105DNA-binding transcriptional regulator BolAPRO_0000201212Add
BLAST

Proteomic databases

PaxDbiP0ABE2.
PRIDEiP0ABE2.

Expressioni

Inductioni

Induced during the transition to the stationary phase, under the control of the sigma factor RpoS (PubMed:2684651). Also induced during early logarithmic growth in response to several forms of stress (heat shock, acidic stress, oxidative stress, carbon-starvation stress and osmotic shock), and this induction can be partially RpoS independent (PubMed:10361282).2 Publications

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
grxDP0AC692EBI-545774,EBI-545828

Protein-protein interaction databases

BioGridi4259840. 527 interactions.
DIPiDIP-47926N.
IntActiP0ABE2. 13 interactions.
MINTiMINT-1238063.
STRINGi511145.b0435.

Structurei

Secondary structure

1
105
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Beta strandi21 – 244Combined sources
Beta strandi39 – 446Combined sources
Helixi46 – 483Combined sources
Helixi55 – 639Combined sources
Helixi65 – 695Combined sources
Beta strandi76 – 805Combined sources
Helixi82 – 865Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DHMNMR-A1-100[»]
ProteinModelPortaliP0ABE2.
SMRiP0ABE2. Positions 1-100.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABE2.

Family & Domainsi

Sequence similaritiesi

Belongs to the BolA/IbaG family.Curated

Phylogenomic databases

eggNOGiENOG4105M85. Bacteria.
COG0271. LUCA.
HOGENOMiHOG000255169.
InParanoidiP0ABE2.
KOiK05527.
OMAiINESHNH.
PhylomeDBiP0ABE2.

Family and domain databases

Gene3Di3.30.300.90. 1 hit.
InterProiIPR002634. BolA.
[Graphical view]
PfamiPF01722. BolA. 1 hit.
[Graphical view]
PIRSFiPIRSF003113. BolA. 1 hit.
SUPFAMiSSF82657. SSF82657. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMIRERIEEK LRAAFQPVFL EVVDESYRHN VPAGSESHFK VVLVSDRFTG
60 70 80 90 100
ERFLNRHRMI YSTLAEELST TVHALALHTY TIKEWEGLQD TVFASPPCRG

AGSIA
Length:105
Mass (Da):11,994
Last modified:October 25, 2005 - v1
Checksum:i7499BFB6228E66F3
GO

Sequence cautioni

The sequence AAB28882 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAB40191 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA35633 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17642 Genomic DNA. Translation: CAA35633.1. Different initiation.
U82664 Genomic DNA. Translation: AAB40191.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73538.2.
AP009048 Genomic DNA. Translation: BAE76215.1.
S67816 Genomic DNA. Translation: AAB28882.2. Different initiation.
RefSeqiNP_414969.4. NC_000913.3.
WP_000973448.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73538; AAC73538; b0435.
BAE76215; BAE76215; BAE76215.
GeneIDi947043.
KEGGiecj:JW5060.
eco:b0435.
PATRICi32116021. VBIEscCol129921_0452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17642 Genomic DNA. Translation: CAA35633.1. Different initiation.
U82664 Genomic DNA. Translation: AAB40191.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73538.2.
AP009048 Genomic DNA. Translation: BAE76215.1.
S67816 Genomic DNA. Translation: AAB28882.2. Different initiation.
RefSeqiNP_414969.4. NC_000913.3.
WP_000973448.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DHMNMR-A1-100[»]
ProteinModelPortaliP0ABE2.
SMRiP0ABE2. Positions 1-100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259840. 527 interactions.
DIPiDIP-47926N.
IntActiP0ABE2. 13 interactions.
MINTiMINT-1238063.
STRINGi511145.b0435.

Proteomic databases

PaxDbiP0ABE2.
PRIDEiP0ABE2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73538; AAC73538; b0435.
BAE76215; BAE76215; BAE76215.
GeneIDi947043.
KEGGiecj:JW5060.
eco:b0435.
PATRICi32116021. VBIEscCol129921_0452.

Organism-specific databases

EchoBASEiEB0123.
EcoGeneiEG10125. bolA.

Phylogenomic databases

eggNOGiENOG4105M85. Bacteria.
COG0271. LUCA.
HOGENOMiHOG000255169.
InParanoidiP0ABE2.
KOiK05527.
OMAiINESHNH.
PhylomeDBiP0ABE2.

Enzyme and pathway databases

BioCyciEcoCyc:EG10125-MONOMER.
ECOL316407:JW5060-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ABE2.
PROiP0ABE2.

Family and domain databases

Gene3Di3.30.300.90. 1 hit.
InterProiIPR002634. BolA.
[Graphical view]
PfamiPF01722. BolA. 1 hit.
[Graphical view]
PIRSFiPIRSF003113. BolA. 1 hit.
SUPFAMiSSF82657. SSF82657. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBOLA_ECOLI
AccessioniPrimary (citable) accession number: P0ABE2
Secondary accession number(s): P15298, Q2MBZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Was predicted, by combining genome sequences, physical interactions and 3D structures analyses, to be a reductase that interacts with a glutaredoxin.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.