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Protein

DNA-binding transcriptional regulator BolA

Gene

bolA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator that plays an important role in general stress response. Has many effects on cell morphology, cell growth and cell division. Acts by regulating the transcription of many genes, including dacA (PBP-5), dacC (PBP-6), ampC and mreB. Probably involved in the coordination of genes that adapt the cell physiology in order to enhance cell adaptation and survival under stress conditions. Essential for normal cell morphology in stationary phase and under conditions of starvation (PubMed:10361282, PubMed:12354237, PubMed:19111750, PubMed:21464593, PubMed:25691594). Also regulates a complex network of genes encoding proteins related to biofilm development, and negatively modulates flagellar biosynthesis and swimming capacity. Could be a motile/adhesive transcriptional switch, specifically involved in the transition between the planktonic and the attachment stage of biofilm formation (PubMed:25691594). Overexpression produces round cell shape, impairs cell growth rate and induces biofilm development (PubMed:305364, PubMed:15345459, PubMed:21464593).7 Publications

Miscellaneous

Was predicted, by combining genome sequences, physical interactions and 3D structures analyses, to be a reductase that interacts with a glutaredoxin.1 Publication

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processStress response, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG10125-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding transcriptional regulator BolACurated
Gene namesi
Name:bolA1 Publication
Ordered Locus Names:b0435, JW5060
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10125 bolA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002012121 – 105DNA-binding transcriptional regulator BolAAdd BLAST105

Proteomic databases

PaxDbiP0ABE2
PRIDEiP0ABE2

Expressioni

Inductioni

Induced during the transition to the stationary phase, under the control of the sigma factor RpoS (PubMed:2684651). Also induced during early logarithmic growth in response to several forms of stress (heat shock, acidic stress, oxidative stress, carbon-starvation stress and osmotic shock), and this induction can be partially RpoS independent (PubMed:10361282).2 Publications

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4259840, 529 interactors
DIPiDIP-47926N
IntActiP0ABE2, 13 interactors
STRINGi316385.ECDH10B_0391

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 14Combined sources12
Beta strandi21 – 24Combined sources4
Beta strandi39 – 44Combined sources6
Helixi46 – 48Combined sources3
Helixi55 – 63Combined sources9
Helixi65 – 69Combined sources5
Beta strandi76 – 80Combined sources5
Helixi82 – 86Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DHMNMR-A1-100[»]
ProteinModelPortaliP0ABE2
SMRiP0ABE2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABE2

Family & Domainsi

Sequence similaritiesi

Belongs to the BolA/IbaG family.Curated

Phylogenomic databases

eggNOGiENOG4105M85 Bacteria
COG0271 LUCA
HOGENOMiHOG000255169
InParanoidiP0ABE2
KOiK05527
OMAiSKKTRGT
PhylomeDBiP0ABE2

Family and domain databases

InterProiView protein in InterPro
IPR002634 BolA
IPR036065 BolA-like_sf
PANTHERiPTHR12735 PTHR12735, 1 hit
PfamiView protein in Pfam
PF01722 BolA, 1 hit
PIRSFiPIRSF003113 BolA, 1 hit
SUPFAMiSSF82657 SSF82657, 1 hit

Sequencei

Sequence statusi: Complete.

P0ABE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMIRERIEEK LRAAFQPVFL EVVDESYRHN VPAGSESHFK VVLVSDRFTG
60 70 80 90 100
ERFLNRHRMI YSTLAEELST TVHALALHTY TIKEWEGLQD TVFASPPCRG

AGSIA
Length:105
Mass (Da):11,994
Last modified:October 25, 2005 - v1
Checksum:i7499BFB6228E66F3
GO

Sequence cautioni

The sequence AAB28882 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAB40191 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA35633 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17642 Genomic DNA Translation: CAA35633.1 Different initiation.
U82664 Genomic DNA Translation: AAB40191.1 Different initiation.
U00096 Genomic DNA Translation: AAC73538.2
AP009048 Genomic DNA Translation: BAE76215.1
S67816 Genomic DNA Translation: AAB28882.2 Different initiation.
RefSeqiNP_414969.4, NC_000913.3
WP_000973448.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73538; AAC73538; b0435
BAE76215; BAE76215; BAE76215
GeneIDi947043
KEGGiecj:JW5060
eco:b0435
PATRICifig|511145.12.peg.452

Similar proteinsi

Entry informationi

Entry nameiBOLA_ECOLI
AccessioniPrimary (citable) accession number: P0ABE2
Secondary accession number(s): P15298, Q2MBZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 25, 2005
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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