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Protein

Modulator of FtsH protease HflC

Gene

hflC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

HflC and HflK help govern the stability of phage lambda cII protein, and thereby control the lysogenization frequency of phage lambda. HflKC inhibits the SecY-degrading activity of FtsH, possibly helping quality control of integral membrane proteins.1 Publication

GO - Biological processi

  • regulation of peptidase activity Source: InterPro
  • response to heat Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG10435-MONOMER.
ECOL316407:JW4133-MONOMER.
MetaCyc:EG10435-MONOMER.

Protein family/group databases

MEROPSiI87.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Modulator of FtsH protease HflC
Gene namesi
Name:hflC
Synonyms:hflA
Ordered Locus Names:b4175, JW4133
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10435. hflC.

Subcellular locationi

  • Cell inner membrane 1 Publication; Single-pass type II membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 2Cytoplasmic1 Publication2
Transmembranei3 – 23Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST21
Topological domaini24 – 334Periplasmic1 PublicationAdd BLAST311

GO - Cellular componenti

  • integral component of external side of plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi145G → A in hflC9; stabilizes overproduced SecY but not overproduced cII protein. 1 Publication1
Mutagenesisi165 – 200Missing : No effect on phage lambda lysogenization frequency. 1 PublicationAdd BLAST36

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000940731 – 334Modulator of FtsH protease HflCAdd BLAST334

Proteomic databases

EPDiP0ABC3.
PaxDbiP0ABC3.
PRIDEiP0ABC3.

Interactioni

Subunit structurei

HflC and HflK interact to form a complex, originally called HflA, now called HflKC. HflKC interacts with FtsH; complex formation is stimulated by ATP, and with YccA.3 Publications

Protein-protein interaction databases

BioGridi4262706. 187 interactors.
DIPiDIP-35995N.
IntActiP0ABC3. 8 interactors.
MINTiMINT-1256053.
STRINGi511145.b4175.

Structurei

3D structure databases

ProteinModelPortaliP0ABC3.
SMRiP0ABC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the band 7/mec-2 family. HflC subfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EPW. Bacteria.
COG0330. LUCA.
HOGENOMiHOG000260972.
InParanoidiP0ABC3.
KOiK04087.
OMAiTRVDYVE.
PhylomeDBiP0ABC3.

Family and domain databases

CDDicd03405. SPFH_HflC. 1 hit.
InterProiIPR001107. Band_7.
IPR010200. HflC.
IPR001972. Stomatin_fam.
[Graphical view]
PANTHERiPTHR10264. PTHR10264. 2 hits.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PIRSFiPIRSF005651. HflC. 1 hit.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 2 hits.
TIGRFAMsiTIGR01932. hflC. 1 hit.

Sequencei

Sequence statusi: Complete.

P0ABC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKSVIAIII IVLVVLYMSV FVVKEGERGI TLRFGKVLRD DDNKPLVYEP
60 70 80 90 100
GLHFKIPFIE TVKMLDARIQ TMDNQADRFV TKEKKDLIVD SYIKWRISDF
110 120 130 140 150
SRYYLATGGG DISQAEVLLK RKFSDRLRSE IGRLDVKDIV TDSRGRLTLE
160 170 180 190 200
VRDALNSGSA GTEDEVTTPA ADNAIAEAAE RVTAETKGKV PVINPNSMAA
210 220 230 240 250
LGIEVVDVRI KQINLPTEVS EAIYNRMRAE REAVARRHRS QGQEEAEKLR
260 270 280 290 300
ATADYEVTRT LAEAERQGRI MRGEGDAEAA KLFADAFSKD PDFYAFIRSL
310 320 330
RAYENSFSGN QDVMVMSPDS DFFRYMKTPT SATR
Length:334
Mass (Da):37,650
Last modified:November 8, 2005 - v1
Checksum:iFC2B0AAC65D27946
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00005 Unassigned DNA. Translation: AAC43400.1.
U14003 Genomic DNA. Translation: AAA97071.1.
U00096 Genomic DNA. Translation: AAC77132.1.
AP009048 Genomic DNA. Translation: BAE78176.1.
PIRiC43653.
RefSeqiNP_418596.1. NC_000913.3.
WP_001232412.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77132; AAC77132; b4175.
BAE78176; BAE78176; BAE78176.
GeneIDi948697.
KEGGiecj:JW4133.
eco:b4175.
PATRICi32123923. VBIEscCol129921_4306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00005 Unassigned DNA. Translation: AAC43400.1.
U14003 Genomic DNA. Translation: AAA97071.1.
U00096 Genomic DNA. Translation: AAC77132.1.
AP009048 Genomic DNA. Translation: BAE78176.1.
PIRiC43653.
RefSeqiNP_418596.1. NC_000913.3.
WP_001232412.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0ABC3.
SMRiP0ABC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262706. 187 interactors.
DIPiDIP-35995N.
IntActiP0ABC3. 8 interactors.
MINTiMINT-1256053.
STRINGi511145.b4175.

Protein family/group databases

MEROPSiI87.001.

Proteomic databases

EPDiP0ABC3.
PaxDbiP0ABC3.
PRIDEiP0ABC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77132; AAC77132; b4175.
BAE78176; BAE78176; BAE78176.
GeneIDi948697.
KEGGiecj:JW4133.
eco:b4175.
PATRICi32123923. VBIEscCol129921_4306.

Organism-specific databases

EchoBASEiEB0430.
EcoGeneiEG10435. hflC.

Phylogenomic databases

eggNOGiENOG4105EPW. Bacteria.
COG0330. LUCA.
HOGENOMiHOG000260972.
InParanoidiP0ABC3.
KOiK04087.
OMAiTRVDYVE.
PhylomeDBiP0ABC3.

Enzyme and pathway databases

BioCyciEcoCyc:EG10435-MONOMER.
ECOL316407:JW4133-MONOMER.
MetaCyc:EG10435-MONOMER.

Miscellaneous databases

PROiP0ABC3.

Family and domain databases

CDDicd03405. SPFH_HflC. 1 hit.
InterProiIPR001107. Band_7.
IPR010200. HflC.
IPR001972. Stomatin_fam.
[Graphical view]
PANTHERiPTHR10264. PTHR10264. 2 hits.
PfamiPF01145. Band_7. 1 hit.
[Graphical view]
PIRSFiPIRSF005651. HflC. 1 hit.
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 2 hits.
TIGRFAMsiTIGR01932. hflC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHFLC_ECOLI
AccessioniPrimary (citable) accession number: P0ABC3
Secondary accession number(s): P25661, Q2M6D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Integration of this protein into the membrane depends on SecA, SecY and SecD but not on SecB or FtsY. HflC is unstable in the absence of HflK.

Caution

Was originally (PubMed:2973057) thought to be a protease. However, removal of residues 165-200 (a ClpP-protease-like motif) does not alter the lysogenization process, and in vitro studies show no evidence of a protease activity for the isolated HflKC complex.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.