Magnesium-transporting ATPase, P-type 1 - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0ABB8 (ATMA_ECOLI)

Last modified February 9, 2010. Version 46. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Magnesium-transporting ATPase, P-type 1
    EC=3.6.3.2
Alternative name(s):
    Mg(2+) transport ATPase, P-type 1

Gene names

Name:	mgtA
Synonyms:	corB, mgt
Ordered Locus Names:	b4242, JW4201

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	898 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Mediates magnesium influx to the cytosol.
Catalytic activity	ATP + H₂O + Mg²⁺(Out) = ADP + phosphate + Mg²⁺(In).
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Induction	Induced by low extracellular levels of magnesium via the phoQ/phoP two-component regulatory system. Ref.4
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIIB subfamily.

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Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro magnesium ion transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium-importing ATPase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
pflB	P09373	1	EBI-556753,EBI-546682

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 898

898

Magnesium-transporting ATPase, P-type 1

PRO_0000046182

Regions

Topological domain

1 – 94

Cytoplasmic Potential

Transmembrane

95 – 115

1 Potential

Topological domain

116

Extracellular Potential

Transmembrane

117 – 137

2 Potential

Topological domain

138 – 287

150

Cytoplasmic Potential

Transmembrane

288 – 308

3 Potential

Topological domain

309 – 317

Extracellular Potential

Transmembrane

318 – 335

4 Potential

Topological domain

336 – 695

360

Cytoplasmic Potential

Transmembrane

696 – 715

5 Potential

Topological domain

716 – 724

Extracellular Potential

Transmembrane

725 – 744

6 Potential

Topological domain

745 – 766

Cytoplasmic Potential

Transmembrane

767 – 790

7 Potential

Topological domain

791 – 799

Extracellular Potential

Transmembrane

800 – 818

8 Potential

Topological domain

819 – 831

Cytoplasmic Potential

Transmembrane

832 – 851

9 Potential

Topological domain

852 – 866

Extracellular Potential

Transmembrane

867 – 886

10 Potential

Topological domain

887 – 898

Cytoplasmic Potential

Sites

Active site

373

4-aspartylphosphate intermediate By similarity

Metal binding

331

Magnesium Potential

Metal binding

641

Magnesium By similarity

Metal binding

645

Magnesium By similarity

Metal binding

709

Magnesium Potential

Metal binding

734

Magnesium Potential

Metal binding

738

Magnesium Potential

Experimental info

Sequence conflict

301 – 317

PVVLL…DWWEA → AGGAVNQWLHQRRLVGS in AAA97139. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P0ABB8-1 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 2B097D67E3FFA956
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FASTA

898

99,466

        10         20         30         40         50         60 
MFKEIFTRLI RHLPSRLVHR DPLPGAQQTV NTVVPPSLSA HCLKMAVMPE EELWKTFDTH 

        70         80         90        100        110        120 
PEGLNQAEVE SAREQHGENK LPAQQPSPWW VHLWVCYRNP FNILLTILGA ISYATEDLFA 

       130        140        150        160        170        180 
AGVIALMVAI STLLNFIQEA RSTKAADALK AMVSNTATVL RVINDKGENG WLEIPIDQLV 

       190        200        210        220        230        240 
PGDIIKLAAG DMIPADLRIL QARDLFVAQA SLTGESLPVE KAATTRQPEH SNPLECDTLC 

       250        260        270        280        290        300 
FMGTTVVSGT AQAMVIATGA NTWFGQLAGR VSEQESEPNA FQQGISRVSM LLIRFMLVMA 

       310        320        330        340        350        360 
PVVLLINGYT KGDWWEAALF ALSVAVGLTP EMLPMIVTST LARGAVKLSK QKVIVKHLDA 

       370        380        390        400        410        420 
IQNFGAMDIL CTDKTGTLTQ DKIVLENHTD ISGKTSERVL HSAWLNSHYQ TGLKNLLDTA 

       430        440        450        460        470        480 
VLEGTDEESA RSLASRWQKI DEIPFDFERR RMSVVVAENT EHHQLVCKGA LQEILNVCSQ 

       490        500        510        520        530        540 
VRHNGEIVPL DDIMLRKIKR VTDTLNRQGL RVVAVATKYL PAREGDYQRA DESDLILEGY 

       550        560        570        580        590        600 
IAFLDPPKET TAPALKALKA SGITVKILTG DSELVAAKVC HEVGLDAGEV VIGSDIETLS 

       610        620        630        640        650        660 
DDELANLAQR TTLFARLTPM HKERIVTLLK REGHVVGFMG DGINDAPALR AADIGISVDG 

       670        680        690        700        710        720 
AVDIAREAAD IILLEKSLMV LEEGVIEGRR TFANMLKYIK MTASSNFGNV FSVLVASAFL 

       730        740        750        760        770        780 
PFLPMLPLHL LIQNLLYDVS QVAIPFDNVD DEQIQKPQRW NPADLGRFMI FFGPISSIFD 

       790        800        810        820        830        840 
ILTFCLMWWV FHANTPETQT LFQSGWFVVG LLSQTLIVHM IRTRRVPFIQ SCASWPLMIM 

       850        860        870        880        890 
TVIVMIVGIA LPFSPLASYL QLQALPLSYF PWLVAILAGY MTLTQLVKGF YSRRYGWQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 301-317. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli." Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T., Mori H., Ishihama A., Utsumi R. J. Bacteriol. 185:3696-3702(2003) [PubMed: 12813061] [Abstract] Cited for: INDUCTION. Strain: K12.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U14003 Genomic DNA. Translation: AAA97139.1.
U00096 Genomic DNA. Translation: AAC77199.1.
AP009048 Genomic DNA. Translation: BAE78241.1.

PIR

E65236.

RefSeq

AP_004740.1.
NP_418663.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GWI	X-ray	1.60	A	383-545	[»]

SMR

P0ABB8. Positions 47-895.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0ABB8. 2 interactions.

STRING

P0ABB8.

Proteomic databases

PRIDE

P0ABB8.

Genome annotation databases

GeneID

948778.

GenomeReviews

Gene locus JW4201 in contig AP009048_GR.
Gene locus b4242 in contig U00096_GR.

KEGG

ecj:JW4201.
eco:b4242.

Organism-specific databases

EchoBASE

EB2416.

EcoGene

EG12525. mgtA.

CMR

Search...

Phylogenomic databases

eggNOG

COG0474.

HOGENOM

HBG456486.

OMA

HIFAKLS.

Enzyme and pathway databases

BioCyc

EcoCyc:MGTA-MONOMER.
ECOL168927:B4242-MONOMER.

Gene expression databases

Genevestigator

P0ABB8.

Family and domain databases

InterPro

IPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR001757. ATPase_P-typ_ion-transptr.
IPR006415. ATPase_P-typ_Mg-translocating.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
[Graphical view]

PANTHER

PTHR11939:SF62. ATPase-IIIB_Mg. 1 hit.
PTHR11939. ATPase_P. 1 hit.

Pfam

PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]

PRINTS

PR01836. MGATPASE.

SMART

SM00831. Cation_ATPase_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01524. ATPase-IIIB_Mg. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.

PROSITE

PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

ATMA_ECOLI

Accession

Primary (citable) accession number: P0ABB8
Secondary accession number(s): P39168, Q2M665

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents