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Protein

ATP synthase subunit beta

Gene

atpD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi150 – 157ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ATP synthesis, Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ATPD-MONOMER.
ECOL316407:JW3710-MONOMER.
MetaCyc:ATPD-MONOMER.

Protein family/group databases

TCDBi3.A.2.1.1. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP synthase subunit betaUniRule annotation (EC:3.6.3.14UniRule annotation)
Alternative name(s):
ATP synthase F1 sector subunit betaUniRule annotation
F-ATPase subunit betaUniRule annotation
Gene namesi
Name:atpDUniRule annotation
Synonyms:papB, uncD
Ordered Locus Names:b3732, JW3710
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10101. atpD.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • proton-transporting ATP synthase complex, catalytic core F(1) Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, CF(1), Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157T → A or C: Impairs ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001444372 – 460ATP synthase subunit betaAdd BLAST459

Proteomic databases

EPDiP0ABB4.
PaxDbiP0ABB4.
PRIDEiP0ABB4.

2D gel databases

SWISS-2DPAGEP0ABB4.

Interactioni

Subunit structurei

F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a1, b2 and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF1 is attached to CF0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
atpAP0ABB010EBI-368783,EBI-368707

Protein-protein interaction databases

DIPiDIP-31846N.
IntActiP0ABB4. 31 interactors.
MINTiMINT-1251407.
STRINGi511145.b3732.

Structurei

Secondary structure

1460
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Beta strandi13 – 17Combined sources5
Beta strandi29 – 33Combined sources5
Beta strandi38 – 46Combined sources9
Beta strandi48 – 58Combined sources11
Beta strandi68 – 71Combined sources4
Beta strandi73 – 75Combined sources3
Turni82 – 86Combined sources5
Beta strandi97 – 99Combined sources3
Beta strandi108 – 110Combined sources3
Helixi132 – 136Combined sources5
Beta strandi145 – 150Combined sources6
Helixi156 – 169Combined sources14
Beta strandi175 – 179Combined sources5
Helixi184 – 196Combined sources13
Helixi198 – 200Combined sources3
Beta strandi203 – 207Combined sources5
Helixi214 – 231Combined sources18
Turni232 – 235Combined sources4
Beta strandi237 – 243Combined sources7
Helixi245 – 258Combined sources14
Helixi265 – 267Combined sources3
Helixi272 – 281Combined sources10
Beta strandi290 – 298Combined sources9
Helixi300 – 302Combined sources3
Helixi307 – 313Combined sources7
Beta strandi317 – 322Combined sources6
Helixi324 – 328Combined sources5
Turni337 – 339Combined sources3
Helixi347 – 350Combined sources4
Helixi352 – 377Combined sources26
Helixi380 – 382Combined sources3
Helixi387 – 400Combined sources14
Turni407 – 411Combined sources5
Helixi421 – 431Combined sources11
Turni432 – 434Combined sources3
Helixi435 – 438Combined sources4
Helixi441 – 444Combined sources4
Helixi450 – 459Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D8SX-ray4.40D/E/F1-460[»]
3OAAX-ray3.26D/E/F/L/M/N/T/U/V/b/c/d2-460[»]
ProteinModelPortaliP0ABB4.
SMRiP0ABB4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0ABB4.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4J. Bacteria.
COG0055. LUCA.
HOGENOMiHOG000009605.
InParanoidiP0ABB4.
KOiK02112.
OMAiAEFGIYP.
PhylomeDBiP0ABB4.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01347. ATP_synth_beta_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR005722. ATP_synth_F1_bsu.
IPR020003. ATPase_a/bsu_AS.
IPR004100. ATPase_F1/V1/A1_a/bsu_N.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR024034. ATPase_F1/V1_bsu_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01039. atpD. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0ABB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATGKIVQVI GAVVDVEFPQ DAVPRVYDAL EVQNGNERLV LEVQQQLGGG
60 70 80 90 100
IVRTIAMGSS DGLRRGLDVK DLEHPIEVPV GKATLGRIMN VLGEPVDMKG
110 120 130 140 150
EIGEEERWAI HRAAPSYEEL SNSQELLETG IKVIDLMCPF AKGGKVGLFG
160 170 180 190 200
GAGVGKTVNM MELIRNIAIE HSGYSVFAGV GERTREGNDF YHEMTDSNVI
210 220 230 240 250
DKVSLVYGQM NEPPGNRLRV ALTGLTMAEK FRDEGRDVLL FVDNIYRYTL
260 270 280 290 300
AGTEVSALLG RMPSAVGYQP TLAEEMGVLQ ERITSTKTGS ITSVQAVYVP
310 320 330 340 350
ADDLTDPSPA TTFAHLDATV VLSRQIASLG IYPAVDPLDS TSRQLDPLVV
360 370 380 390 400
GQEHYDTARG VQSILQRYQE LKDIIAILGM DELSEEDKLV VARARKIQRF
410 420 430 440 450
LSQPFFVAEV FTGSPGKYVS LKDTIRGFKG IMEGEYDHLP EQAFYMVGSI
460
EEAVEKAKKL
Length:460
Mass (Da):50,325
Last modified:January 23, 2007 - v2
Checksum:i18DB33154B1AE6FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA. Translation: CAA25782.1.
V00267 Genomic DNA. Translation: CAA23527.1.
M25464 Genomic DNA. Translation: AAA83875.1.
J01594 Genomic DNA. Translation: AAA24737.1.
V00311 Genomic DNA. Translation: CAA23594.1.
V00312 Genomic DNA. Translation: CAA23598.1.
L10328 Genomic DNA. Translation: AAA62084.1.
U00096 Genomic DNA. Translation: AAC76755.1.
AP009048 Genomic DNA. Translation: BAE77556.1.
PIRiA93742. PWECB.
RefSeqiNP_418188.1. NC_000913.3.
WP_000190506.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76755; AAC76755; b3732.
BAE77556; BAE77556; BAE77556.
GeneIDi948244.
KEGGiecj:JW3710.
eco:b3732.
PATRICi32122961. VBIEscCol129921_3856.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01631 Genomic DNA. Translation: CAA25782.1.
V00267 Genomic DNA. Translation: CAA23527.1.
M25464 Genomic DNA. Translation: AAA83875.1.
J01594 Genomic DNA. Translation: AAA24737.1.
V00311 Genomic DNA. Translation: CAA23594.1.
V00312 Genomic DNA. Translation: CAA23598.1.
L10328 Genomic DNA. Translation: AAA62084.1.
U00096 Genomic DNA. Translation: AAC76755.1.
AP009048 Genomic DNA. Translation: BAE77556.1.
PIRiA93742. PWECB.
RefSeqiNP_418188.1. NC_000913.3.
WP_000190506.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D8SX-ray4.40D/E/F1-460[»]
3OAAX-ray3.26D/E/F/L/M/N/T/U/V/b/c/d2-460[»]
ProteinModelPortaliP0ABB4.
SMRiP0ABB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-31846N.
IntActiP0ABB4. 31 interactors.
MINTiMINT-1251407.
STRINGi511145.b3732.

Protein family/group databases

TCDBi3.A.2.1.1. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

2D gel databases

SWISS-2DPAGEP0ABB4.

Proteomic databases

EPDiP0ABB4.
PaxDbiP0ABB4.
PRIDEiP0ABB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76755; AAC76755; b3732.
BAE77556; BAE77556; BAE77556.
GeneIDi948244.
KEGGiecj:JW3710.
eco:b3732.
PATRICi32122961. VBIEscCol129921_3856.

Organism-specific databases

EchoBASEiEB0099.
EcoGeneiEG10101. atpD.

Phylogenomic databases

eggNOGiENOG4105C4J. Bacteria.
COG0055. LUCA.
HOGENOMiHOG000009605.
InParanoidiP0ABB4.
KOiK02112.
OMAiAEFGIYP.
PhylomeDBiP0ABB4.

Enzyme and pathway databases

BioCyciEcoCyc:ATPD-MONOMER.
ECOL316407:JW3710-MONOMER.
MetaCyc:ATPD-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0ABB4.
PROiP0ABB4.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01347. ATP_synth_beta_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR005722. ATP_synth_F1_bsu.
IPR020003. ATPase_a/bsu_AS.
IPR004100. ATPase_F1/V1/A1_a/bsu_N.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR024034. ATPase_F1/V1_bsu_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01039. atpD. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATPB_ECOLI
AccessioniPrimary (citable) accession number: P0ABB4
Secondary accession number(s): P00824, Q2M850
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.