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P0ABA0 (ATPF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP synthase subunit b
Alternative name(s):
ATP synthase F(0) sector subunit b
ATPase subunit I
F-type ATPase subunit b
Short name=F-ATPase subunit b
Gene names
Name:atpF
Synonyms:papF, uncF
Ordered Locus Names:b3736, JW3714
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length156 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

F1F0 ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F1 containing the extramembraneous catalytic core and F0 containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation By similarity. Ref.11

Component of the F0 channel, it forms part of the peripheral stalk, linking F1 to F0 By similarity. Ref.11

Subunit structure

F-type ATPases have 2 components, F1 - the catalytic core - and F0 - the membrane proton channel. F1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. F0 has three main subunits: a1, b2 and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F1 is attached to F0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains By similarity. Ref.12

Subcellular location

Cell inner membrane; Single-pass membrane protein By similarity Ref.12.

Sequence similarities

Belongs to the ATPase B chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 156156ATP synthase subunit b HAMAP-Rule MF_01398
PRO_0000082371

Regions

Transmembrane11 – 3121Helical; Potential

Sites

Site91Mutation prevents the formation of a functional proton pore, but has a small effect on the binding of F(1) to F(0)
Site1311Required for proton pore formation, as well as F(1) to F(0) binding

Experimental info

Sequence conflict331I → F in CAA23592. Ref.3
Sequence conflict621T → N in AAA24741. Ref.7
Sequence conflict721A → D in CAA23592. Ref.3

Secondary structure

....... 156
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0ABA0 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 51A93C8BEE9AD9DF

FASTA15617,264
        10         20         30         40         50         60 
MNLNATILGQ AIAFVLFVLF CMKYVWPPLM AAIEKRQKEI ADGLASAERA HKDLDLAKAS 

        70         80         90        100        110        120 
ATDQLKKAKA EAQVIIEQAN KRRSQILDEA KAEAEQERTK IVAQAQAEIE AERKRAREEL 

       130        140        150 
RKQVAILAVA GAEKIIERSV DEAANSDIVD KLVAEL 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS."
Walker J.E., Gay N.J., Saraste M., Eberle A.N.
Biochem. J. 224:799-815(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The atp operon: nucleotide sequence of the promoter and the genes for the membrane proteins, and the delta subunit of Escherichia coli ATP-synthase."
Gay N.J., Walker J.E.
Nucleic Acids Res. 9:3919-3926(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Nucleotide sequence of the genes for F0 components of the proton-translocating ATPase from Escherichia coli: prediction of the primary structure of F0 subunits."
Kanazawa H., Mabuchi K., Kayano T., Noumi T., Sekiya T., Futai M.
Biochem. Biophys. Res. Commun. 103:613-620(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of the atp genes coding for the F0 subunits a, b, c and the F1 subunit delta of the membrane bound ATP synthase of Escherichia coli."
Nielsen J., Hansen F.G., Hoppe J., Friedl P., von Meyenburg K.
Mol. Gen. Genet. 184:33-39(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Structure and function of H+-ATPase: what we have learned from Escherichia coli H+-ATPase."
Kanazawa H., Futai M.
Ann. N. Y. Acad. Sci. 402:45-64(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Role of the b subunit of the Escherichia coli proton-translocating ATPase. A mutagenic analysis."
Porter A.C.G., Kumamoto C., Aldape K., Simoni R.D.
J. Biol. Chem. 260:8182-8187(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[8]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[9]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[10]"Nucleotide sequence of the gene coding for the delta subunit of proton translocating ATPase of Escherichia coli."
Mabuchi K., Kanazawa H., Kayano T., Futai M.
Biochem. Biophys. Res. Commun. 102:172-179(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 132-156.
[11]"Targeted mutagenesis of the b subunit of F1F0 ATP synthase in Escherichia coli: Glu-77 through Gln-85."
McCormick K.A., Cain B.D.
J. Bacteriol. 173:7240-7248(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Protein complexes of the Escherichia coli cell envelope."
Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.
J. Biol. Chem. 280:34409-34419(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION.
Strain: BL21-DE3.
[13]"Structure of the membrane domain of subunit b of the Escherichia coli F0F1 ATP synthase."
Dmitriev O., Jones P.C., Jiang W., Fillingame R.H.
J. Biol. Chem. 274:15598-15604(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 1-34.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J01594 Genomic DNA. Translation: AAA24733.1.
X01631 Genomic DNA. Translation: CAA25778.1.
V00264 Genomic DNA. Translation: CAA23516.1.
V00310 Genomic DNA. Translation: CAA23592.1.
V00266 Genomic DNA. Translation: CAA23523.1.
M25464 Genomic DNA. Translation: AAA83871.1.
M10422 Genomic DNA. Translation: AAA24741.1.
L10328 Genomic DNA. Translation: AAA62088.1.
U00096 Genomic DNA. Translation: AAC76759.1.
AP009048 Genomic DNA. Translation: BAE77552.1.
M12212 Unassigned DNA. Translation: AAA20043.1.
PIRLWECB. D93732.
RefSeqNP_418192.1. NC_000913.3.
YP_491693.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B9UNMR-A1-34[»]
1L2PX-ray1.55A62-122[»]
2KHKNMR-A30-82[»]
ProteinModelPortalP0ABA0.
SMRP0ABA0. Positions 1-122.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35994N.
IntActP0ABA0. 2 interactions.
STRING511145.b3736.

Protein family/group databases

TCDB3.A.2.1.1. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

2D gel databases

SWISS-2DPAGEP0ABA0.

Proteomic databases

PaxDbP0ABA0.
PRIDEP0ABA0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76759; AAC76759; b3736.
BAE77552; BAE77552; BAE77552.
GeneID12933115.
948247.
KEGGecj:Y75_p3432.
eco:b3736.
PATRIC32122969. VBIEscCol129921_3860.

Organism-specific databases

EchoBASEEB0101.
EcoGeneEG10103. atpF.

Phylogenomic databases

eggNOGCOG0711.
HOGENOMHOG000015378.
KOK02109.
OMAVLPKFQE.
OrthoDBEOG6DNTDK.
PhylomeDBP0ABA0.
ProtClustDBPRK05759.

Enzyme and pathway databases

BioCycEcoCyc:ATPF-MONOMER.
ECOL316407:JW3714-MONOMER.
MetaCyc:ATPF-MONOMER.

Gene expression databases

GenevestigatorP0ABA0.

Family and domain databases

HAMAPMF_01398. ATP_synth_b_bact.
InterProIPR002146. ATPase_F0-cplx_b/b'su_bac.
IPR005864. ATPase_F0-cplx_bsu_bac.
[Graphical view]
PfamPF00430. ATP-synt_B. 1 hit.
[Graphical view]
TIGRFAMsTIGR01144. ATP_synt_b. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0ABA0.
PROP0ABA0.

Entry information

Entry nameATPF_ECOLI
AccessionPrimary (citable) accession number: P0ABA0
Secondary accession number(s): P00859, Q2M854
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene