Reviewed, UniProtKB/Swiss-Prot P0AB96 (ARSC_ECOLI)

Names and origin

Protein names

Recommended name:
    Arsenate reductase
    EC=1.20.4.1
Alternative name(s):
    Arsenical pump modifier

Gene names

Name:	arsC
Synonyms:	arsG
Ordered Locus Names:	b3503, JW3470

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of arsenate [As(V)] to arsenite [As(III)]. This protein expands the substrate specificity of arsAB pump which can extrude arsenite and antimonite to allow for arsenate pumping and resistance By similarity.
Catalytic activity	Arsenate + glutaredoxin = arsenite + glutaredoxin disulfide + H2O.
Sequence similarities	Belongs to the arsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (glutaredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 141	141	Arsenate reductase		PRO_0000162536

Sequences

	Sequence		Length	Mass (Da)
	P0AB96-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 5490FBBD14B24D3C	FASTA	141	15,853
	10 20 30 40 50 60 MSNITIYHNP ACGTSRNTLE MIRNSGTEPT IIHYLETPPT RDELVKLIAD MGISVRALLR 70 80 90 100 110 120 KNVEPYEELG LAEDKFTDDR LIDFMLQHPI LINRPIVVTP LGTRLCRPSE VVLEILPDAQ 130 140 KGAFSKEDGE KVVDEAGKRL K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram-negative bacteria." Diorio C., Cai J., Marmor J., Shinder R., Dubow M.S. J. Bacteriol. 177:2050-2056(1995) [PubMed: 7721697] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X80057 Genomic DNA. Translation: CAA56363.1. U00039 Genomic DNA. Translation: AAB18479.1. U00096 Genomic DNA. Translation: AAC76528.1. AP009048 Genomic DNA. Translation: BAE77791.1.
PIR	C56269.
RefSeq	AP_004290.1. NP_417960.1.
3D structure databases
HSSP	HSSP built from PDB template 1I9D based on UniProtKB P08692.
SMR	P0AB96. Positions 3-140.
ModBase	Search...
Genome annotation databases
GeneID	948018.
GenomeReviews	Gene locus JW3470 in contig AP009048_GR. Gene locus b3503 in contig U00096_GR.
KEGG	ecj:JW3470. eco:b3503.
Organism-specific databases
EchoBASE	EB2149.
EcoGene	EG12237. arsC.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AB96.
OMA	P0AB96. IRNSGNE.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12237-MON.
Family and domain databases
InterPro	IPR006659. Arsenate_reductase. IPR006660. Arsenate_reductase-like. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00014. arsC. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARSC_ECOLI

Accession

Primary (citable) accession number: P0AB96
Secondary accession number(s): P37311, Q2M7G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)