Reviewed, UniProtKB/Swiss-Prot P0AB92 (AROG_ECO57)

Names and origin

Protein names

Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive
    EC=2.5.1.54
Alternative name(s):
    Phospho-2-keto-3-deoxyheptonate aldolase
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
    DAHP synthetase

Gene names

Name:	aroG
Ordered Locus Names:	Z0924, ECs0782

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) By similarity.
Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Subunit structure	Homotetramer By similarity.
Miscellaneous	There are 3 DAHP synthases, aroG is feedback-inhibited by Phe. The other 2 DAHP synthases are Tyr- and Trp-sensitive, respectively.
Sequence similarities	Belongs to the class-I DAHP synthetase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Transferase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive		PRO_0000140836
Amino acid modifications
	Modified residue	244	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AB92-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 7477D361962E8710	FASTA	350	38,010
	10 20 30 40 50 60 MNYQNDDLRI KEIKELLPPV ALLEKFPATE NAANTVAHAR KAIHKILKGN DDRLLVVIGP 70 80 90 100 110 120 CSIHDPVAAK EYATRLLALR EELKDELEIV MRVYFEKPRT TVGWKGLIND PHMDNSFQIN 130 140 150 160 170 180 DGLRIARKLL LDINDSGLPA AGEFLDMITP QYLADLMSWG AIGARTTESQ VHRELASGLS 190 200 210 220 230 240 CPVGFKNGTD GTIKVAIDAI NAAGAPHCFL SVTKWGHSAI VNTSGNGDCH IILRGGKEPN 250 260 270 280 290 300 YSAKHVAEVK EGLNKAGLPA QVMIDFSHAN SSKQFKKQMD VCADVCQQIA GGEKAIIGVM 310 320 330 340 350 VESHLVEGNQ SLESGEPLAY GKSITDACIG WEDTDALLRQ LANAVKARRG

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG55083.1. BA000007 Genomic DNA. Translation: BAB34205.1.
PIR	F90726. G85577.
RefSeq	NP_286475.1. NP_308809.1.
3D structure databases
SMR	P0AB92. Positions 8-350.
ModBase	Search...
Genome annotation databases
GeneID	917531. 957894.
GenomeReviews	Gene locus Z0924 in contig AE005174_GR. Gene locus ECs0782 in contig BA000007_GR.
KEGG	ece:Z0924. ecs:ECs0782.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0AB92.
OMA	MDNSFKL.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0782-MON.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006219. DHAP_synth_1. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21225. AroFGH. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
PIRSF	PIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMs	TIGR00034. aroFGH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROG_ECO57

Accession

Primary (citable) accession number: P0AB92
Secondary accession number(s): P00886

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)