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P0AB80

- ILVE_ECOLI

UniProt

P0AB80 - ILVE_ECOLI

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Protein
Branched-chain-amino-acid aminotransferase
Gene
ilvE, b3770, JW5606
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts on leucine, isoleucine and valine.

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.

Cofactori

Pyridoxal phosphate.

Pathwayi

GO - Molecular functioni

  1. L-isoleucine transaminase activity Source: UniProtKB-EC
  2. L-leucine transaminase activity Source: UniProtKB-EC
  3. L-valine transaminase activity Source: UniProtKB-EC
  4. branched-chain-amino-acid transaminase activity Source: EcoCyc
  5. identical protein binding Source: EcoCyc

GO - Biological processi

  1. aspartate biosynthetic process Source: EcoliWiki
  2. isoleucine biosynthetic process Source: UniProtKB-UniPathway
  3. leucine biosynthetic process Source: EcoCyc
  4. valine biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:BRANCHED-CHAINAMINOTRANSFER-MONOMER.
ECOL316407:JW5606-MONOMER.
MetaCyc:BRANCHED-CHAINAMINOTRANSFER-MONOMER.
UniPathwayiUPA00047; UER00058.
UPA00048; UER00073.
UPA00049; UER00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase (EC:2.6.1.42)
Short name:
BCAT
Alternative name(s):
Transaminase B
Gene namesi
Name:ilvE
Ordered Locus Names:b3770, JW5606
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10497. ilvE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 309308Branched-chain-amino-acid aminotransferase
PRO_0000103262Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei160 – 1601N6-(pyridoxal phosphate)lysine

Proteomic databases

PaxDbiP0AB80.
PRIDEiP0AB80.

2D gel databases

SWISS-2DPAGEP0AB80.

Expressioni

Gene expression databases

GenevestigatoriP0AB80.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

DIPiDIP-10022N.
STRINGi511145.b3770.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115
Beta strandi14 – 174
Helixi18 – 203
Helixi28 – 325
Beta strandi35 – 373
Beta strandi40 – 434
Beta strandi48 – 525
Helixi54 – 6815
Helixi76 – 8914
Beta strandi93 – 10311
Beta strandi108 – 1114
Beta strandi118 – 1258
Helixi135 – 1384
Beta strandi140 – 1445
Turni152 – 1543
Beta strandi157 – 1593
Helixi162 – 1643
Helixi165 – 17713
Beta strandi181 – 1866
Beta strandi190 – 1956
Beta strandi198 – 2047
Beta strandi207 – 2104
Helixi213 – 2153
Helixi221 – 23212
Beta strandi237 – 2393
Helixi246 – 2494
Beta strandi251 – 2577
Turni258 – 2603
Beta strandi261 – 2688
Helixi274 – 2763
Helixi280 – 29011
Turni291 – 2944
Beta strandi295 – 2973
Beta strandi304 – 3063

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A3GX-ray2.50A/B/C2-309[»]
1I1KX-ray2.10A/B/C1-309[»]
1I1LX-ray2.40A/B/C1-309[»]
1I1MX-ray2.40A/B/C1-309[»]
1IYDX-ray2.15A/B/C1-309[»]
1IYEX-ray1.82A/B/C1-309[»]
ProteinModelPortaliP0AB80.
SMRiP0AB80. Positions 5-308.

Miscellaneous databases

EvolutionaryTraceiP0AB80.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0115.
HOGENOMiHOG000276706.
KOiK00826.
OMAiFPWGSYL.
OrthoDBiEOG67MF3R.
PhylomeDBiP0AB80.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005785. B_amino_transI.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01122. ilvE_I. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AB80-1 [UniParc]FASTAAdd to Basket

« Hide

MTTKKADYIW FNGEMVRWED AKVHVMSHAL HYGTSVFEGI RCYDSHKGPV    50
VFRHREHMQR LHDSAKIYRF PVSQSIDELM EACRDVIRKN NLTSAYIRPL 100
IFVGDVGMGV NPPAGYSTDV IIAAFPWGAY LGAEALEQGI DAMVSSWNRA 150
APNTIPTAAK AGGNYLSSLL VGSEARRHGY QEGIALDVNG YISEGAGENL 200
FEVKDGVLFT PPFTSSALPG ITRDAIIKLA KELGIEVREQ VLSRESLYLA 250
DEVFMSGTAA EITPVRSVDG IQVGEGRCGP VTKRIQQAFF GLFTGETEDK 300
WGWLDQVNQ 309
Length:309
Mass (Da):34,094
Last modified:January 23, 2007 - v2
Checksum:iE8A2B953168CD09D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511A → R in AAA67573. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02413 Genomic DNA. Translation: CAA26262.1.
M32253 Genomic DNA. Translation: AAA24022.1.
M10313 Genomic DNA. Translation: AAB59052.1.
X04890 Genomic DNA. Translation: CAA28575.1.
M87049 Genomic DNA. Translation: AAA67573.1.
U00096 Genomic DNA. Translation: AAT48207.1.
AP009048 Genomic DNA. Translation: BAE77527.1.
V00290 Genomic DNA. Translation: CAA23559.1.
PIRiE65180. XNECV.
RefSeqiYP_026247.1. NC_000913.3.
YP_491668.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAT48207; AAT48207; b3770.
BAE77527; BAE77527; BAE77527.
GeneIDi12932278.
948278.
KEGGiecj:Y75_p3405.
eco:b3770.
PATRICi32123035. VBIEscCol129921_3887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02413 Genomic DNA. Translation: CAA26262.1 .
M32253 Genomic DNA. Translation: AAA24022.1 .
M10313 Genomic DNA. Translation: AAB59052.1 .
X04890 Genomic DNA. Translation: CAA28575.1 .
M87049 Genomic DNA. Translation: AAA67573.1 .
U00096 Genomic DNA. Translation: AAT48207.1 .
AP009048 Genomic DNA. Translation: BAE77527.1 .
V00290 Genomic DNA. Translation: CAA23559.1 .
PIRi E65180. XNECV.
RefSeqi YP_026247.1. NC_000913.3.
YP_491668.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A3G X-ray 2.50 A/B/C 2-309 [» ]
1I1K X-ray 2.10 A/B/C 1-309 [» ]
1I1L X-ray 2.40 A/B/C 1-309 [» ]
1I1M X-ray 2.40 A/B/C 1-309 [» ]
1IYD X-ray 2.15 A/B/C 1-309 [» ]
1IYE X-ray 1.82 A/B/C 1-309 [» ]
ProteinModelPortali P0AB80.
SMRi P0AB80. Positions 5-308.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10022N.
STRINGi 511145.b3770.

2D gel databases

SWISS-2DPAGE P0AB80.

Proteomic databases

PaxDbi P0AB80.
PRIDEi P0AB80.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT48207 ; AAT48207 ; b3770 .
BAE77527 ; BAE77527 ; BAE77527 .
GeneIDi 12932278.
948278.
KEGGi ecj:Y75_p3405.
eco:b3770.
PATRICi 32123035. VBIEscCol129921_3887.

Organism-specific databases

EchoBASEi EB0492.
EcoGenei EG10497. ilvE.

Phylogenomic databases

eggNOGi COG0115.
HOGENOMi HOG000276706.
KOi K00826.
OMAi FPWGSYL.
OrthoDBi EOG67MF3R.
PhylomeDBi P0AB80.

Enzyme and pathway databases

UniPathwayi UPA00047 ; UER00058 .
UPA00048 ; UER00073 .
UPA00049 ; UER00062 .
BioCyci EcoCyc:BRANCHED-CHAINAMINOTRANSFER-MONOMER.
ECOL316407:JW5606-MONOMER.
MetaCyc:BRANCHED-CHAINAMINOTRANSFER-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0AB80.
PROi P0AB80.

Gene expression databases

Genevestigatori P0AB80.

Family and domain databases

InterProi IPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005785. B_amino_transI.
[Graphical view ]
PANTHERi PTHR11825. PTHR11825. 1 hit.
Pfami PF01063. Aminotran_4. 1 hit.
[Graphical view ]
SUPFAMi SSF56752. SSF56752. 1 hit.
TIGRFAMsi TIGR01122. ilvE_I. 1 hit.
PROSITEi PS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence."
    Kuramitsu S., Ogawa T., Ogawa H., Kagamiyama H.
    J. Biochem. 97:993-999(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12."
    Lawther R.P., Wek R.C., Lopes J.M., Pereira R., Taillon B.E., Hatfield G.W.
    Nucleic Acids Res. 15:2137-2155(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: SEQUENCE REVISION TO 151.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "The nucleotide sequence preceding and including the beginning of the ilvE gene of the ilvGEDA operon of Escherichia coli K12."
    Lawther R.P., Nichols B.P., Zurawski G., Hatfield G.W.
    Nucleic Acids Res. 7:2289-2301(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-81.
    Strain: K12.
  8. "DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli."
    Pagel J.M., Winkelman J.W., Adams C.W., Hatfield G.W.
    J. Mol. Biol. 224:919-935(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34.
  9. "Branched-chain amino acid aminotransferase of Escherichia coli: overproduction and properties."
    Inoue K., Kuramitsu S., Aki K., Watanabe Y., Takagi T., Nishigai M., Ikaiu A., Kagamiyama H.
    J. Biochem. 104:777-784(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PYRIDOXAL PHOSPHATE AT LYS-160.
  10. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  11. "Three-dimensional structure of Escherichia coli branched-chain amino acid aminotransferase at 2.5-A resolution."
    Okada K., Hirotsu K., Sato M., Hyashi H., Kagamiyama H.
    J. Biochem. 121:637-641(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiILVE_ECOLI
AccessioniPrimary (citable) accession number: P0AB80
Secondary accession number(s): P00510, Q2M879, Q47299
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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