Reviewed, UniProtKB/Swiss-Prot P0AB68 (PNTB_ECOL6)

Names and origin

Protein names

Recommended name:
    NAD(P) transhydrogenase subunit beta
    EC=1.6.1.2
Alternative name(s):
    Nicotinamide nucleotide transhydrogenase subunit beta
    Pyridine nucleotide transhydrogenase subunit beta

Gene names

Name:	pntB
Ordered Locus Names:	c1994

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane By similarity.
Catalytic activity	NADPH + NAD+ = NADP+ + NADH.
Subunit structure	Heterodimer of an alpha and a beta chain By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the PNT beta subunit family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD(P)+ transhydrogenase (AB-specific) activity Inferred from electronic annotation. Source: EC NADP or NADPH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	NAD(P) transhydrogenase subunit beta		PRO_0000199025
Regions
	Topological domain	1 – 3	3	Periplasmic Potential
	Transmembrane	4 – 24	21	Potential
	Topological domain	25 – 45	21	Cytoplasmic Potential
	Transmembrane	46 – 66	21	Potential
	Topological domain	67 – 82	16	Periplasmic Potential
	Transmembrane	83 – 103	21	Potential
	Topological domain	104 – 115	12	Cytoplasmic Potential
	Transmembrane	116 – 136	21	Potential
	Topological domain	137 – 164	28	Periplasmic Potential
	Transmembrane	165 – 185	21	Potential
	Topological domain	186 – 188	3	Cytoplasmic Potential
	Transmembrane	189 – 209	21	Potential
	Topological domain	210 – 215	6	Periplasmic Potential
	Transmembrane	216 – 236	21	Potential
	Topological domain	237 – 239	3	Cytoplasmic Potential
	Transmembrane	240 – 260	21	Potential
	Topological domain	261 – 308	48	Periplasmic Potential
	Transmembrane	309 – 329	21	Potential
	Topological domain	330 – 462	133	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AB68-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: D11747291D271A2F	FASTA	462	48,723
	10 20 30 40 50 60 MSGGLVTAAY IVAAILFIFS LAGLSKHETS RQGNNFGIAG MAIALIATIF GPDTGNVGWI 70 80 90 100 110 120 LLAMVIGGAI GIRLAKKVEM TEMPELVAIL HSFVGLAAVL VGFNSYLHHD AGMAPILVNI 130 140 150 160 170 180 HLTEVFLGIF IGAVTFTGSV VAFGKLCGKI SSKPLMLPNR HKMNLAALVV SFLLLIVFVR 190 200 210 220 230 240 TDSVGLQVLA LLIMTAIALV FGWHLVASIG GADMPVVVSM LNSYSGWAAA AAGFMLSNDL 250 260 270 280 290 300 LIVTGALVGS SGAILSYIMC KAMNRSFISV IAGGFGTDGS STGDDQEVGE HREITAEETA 310 320 330 340 350 360 ELLKNSHSVI ITPGYGMAVA QAQYPVAEIT EKLRARGINV RFGIHPVAGR LPGHMNVLLA 370 380 390 400 410 420 EAKVPYDIVL EMDEINDDFA DTDTVLVIGA NDTVNPAAQD DPKSPIAGMP VLEVWKAQNV 430 440 450 460 IVFKRSMNTG YAGVQNPLFF KENTHMLFGD AKASVDAILK AL

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014075 Genomic DNA. Translation: AAN80454.1.
RefSeq	NP_753889.1.
3D structure databases
HSSP	HSSP built from PDB template 1D4O based on UniProtKB P11024.
SMR	P0AB68. Positions 296-462.
ModBase	Search...
Genome annotation databases
GeneID	1036023.
GenomeReviews	Gene locus c1994 in contig AE014075_GR.
KEGG	ecc:c1994.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0AB68.
OMA	VEIYVGI.
Enzyme and pathway databases
BRENDA	1.6.1.2. 292881.
Family and domain databases
InterPro	IPR012136. NADH_DH_b. IPR004003. NADP_transhyd_b. [Graphical view]
Pfam	PF02233. PNTB. 1 hit. [Graphical view]
PIRSF	PIRSF000204. PNTB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNTB_ECOL6

Accession

Primary (citable) accession number: P0AB68
Secondary accession number(s): P07002, P76890

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)