Reviewed, UniProtKB/Swiss-Prot P0AB67 (PNTB_ECOLI)

Names and origin

Protein names

Recommended name:
    NAD(P) transhydrogenase subunit beta
    EC=1.6.1.2
Alternative name(s):
    Nicotinamide nucleotide transhydrogenase subunit beta
    Pyridine nucleotide transhydrogenase subunit beta

Gene names

Name:	pntB
Ordered Locus Names:	b1602, JW1594

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
Catalytic activity	NADPH + NAD+ = NADP+ + NADH.
Subunit structure	Heterodimer of an alpha and a beta chain.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the PNT beta subunit family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+ transhydrogenase (AB-specific) activity Inferred from electronic annotation. Source: EC NADP or NADPH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 462

462

NAD(P) transhydrogenase subunit beta

PRO_0000199023

Regions

Topological domain

1 – 3

3

Periplasmic Potential

Transmembrane

4 – 24

21

Potential

Topological domain

25 – 45

21

Cytoplasmic Potential

Transmembrane

46 – 66

21

Potential

Topological domain

67 – 82

16

Periplasmic Potential

Transmembrane

83 – 103

21

Potential

Topological domain

104 – 115

12

Cytoplasmic Potential

Transmembrane

116 – 136

21

Potential

Topological domain

137 – 164

28

Periplasmic Potential

Transmembrane

165 – 185

21

Potential

Topological domain

186 – 188

3

Cytoplasmic Potential

Transmembrane

189 – 209

21

Potential

Topological domain

210 – 215

6

Periplasmic Potential

Transmembrane

216 – 236

21

Potential

Topological domain

237 – 239

3

Cytoplasmic Potential

Transmembrane

240 – 260

21

Potential

Topological domain

261 – 308

48

Periplasmic Potential

Transmembrane

309 – 329

21

Potential

Topological domain

330 – 462

133

Cytoplasmic Potential

Secondary structure

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462

Helix Strand Turn

Details...

Helix

297 – 305

9

Beta strand

307 – 312

6

Helix

316 – 319

4

Turn

320 – 322

3

Helix

323 – 336

14

Beta strand

339 – 344

6

Beta strand

346 – 353

8

Helix

355 – 362

8

Turn

366 – 368

3

Beta strand

369 – 372

4

Helix

376 – 381

6

Beta strand

383 – 387

5

Helix

391 – 394

4

Helix

396 – 398

3

Beta strand

404 – 406

3

Beta strand

417 – 423

7

Beta strand

425 – 427

3

Turn

437 – 439

3

Beta strand

440 – 447

8

Helix

451 – 461

11

Sequences

	Sequence		Length	Mass (Da)
	P0AB67-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: D11747291D271A2F	FASTA	462	48,723
	10 20 30 40 50 60 MSGGLVTAAY IVAAILFIFS LAGLSKHETS RQGNNFGIAG MAIALIATIF GPDTGNVGWI 70 80 90 100 110 120 LLAMVIGGAI GIRLAKKVEM TEMPELVAIL HSFVGLAAVL VGFNSYLHHD AGMAPILVNI 130 140 150 160 170 180 HLTEVFLGIF IGAVTFTGSV VAFGKLCGKI SSKPLMLPNR HKMNLAALVV SFLLLIVFVR 190 200 210 220 230 240 TDSVGLQVLA LLIMTAIALV FGWHLVASIG GADMPVVVSM LNSYSGWAAA AAGFMLSNDL 250 260 270 280 290 300 LIVTGALVGS SGAILSYIMC KAMNRSFISV IAGGFGTDGS STGDDQEVGE HREITAEETA 310 320 330 340 350 360 ELLKNSHSVI ITPGYGMAVA QAQYPVAEIT EKLRARGINV RFGIHPVAGR LPGHMNVLLA 370 380 390 400 410 420 EAKVPYDIVL EMDEINDDFA DTDTVLVIGA NDTVNPAAQD DPKSPIAGMP VLEVWKAQNV 430 440 450 460 IVFKRSMNTG YAGVQNPLFF KENTHMLFGD AKASVDAILK AL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli." Clarke D.M., Loo T.W., Gillam S., Bragg P.D. Eur. J. Biochem. 158:647-653(1986) [PubMed: 3525165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A mutation at Gly314 of the beta subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H)-induced conformational change." Ahmad S., Glavas N.A., Bragg P.D. Eur. J. Biochem. 207:733-739(1992) [PubMed: 1633824] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Topological analysis of the pyridine nucleotide transhydrogenase of Escherichia coli using proteolytic enzymes." Tong R.C., Glavas N.A., Bragg P.D. Biochim. Biophys. Acta 1080:19-28(1991) [PubMed: 1932078] [Abstract] Cited for: PROTEIN SEQUENCE OF 266-277 AND 364-373.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X04195 Genomic DNA. Translation: CAB37090.1.
X66086 Genomic DNA. Translation: CAA46885.1.
U00096 Genomic DNA. Translation: AAC74674.1.
AP009048 Genomic DNA. Translation: BAA15336.1.

PIR

DEECXB. S24381.

RefSeq

AP_002223.1.
NP_416119.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BRU	NMR	-	C	286-462	[»]

ModBase

Search...

Protein family/group databases

TCDB

3.D.2.1.1. proton-translocating transhydrogenase (PTH) family.

Genome annotation databases

GeneID

946144.

GenomeReviews

Gene locus JW1594 in contig AP009048_GR.
Gene locus b1602 in contig U00096_GR.

KEGG

ecj:JW1594.
eco:b1602.

Organism-specific databases

EchoBASE

EB0738.

EcoGene

EG10745. pntB.

CMR

Search...

Phylogenomic databases

HOGENOM

P0AB67.

OMA

P0AB67. VEIYVGI.

Enzyme and pathway databases

BioCyc

EcoCyc:PNTB-MON.
MetaCyc:PNTB-MON.

Family and domain databases

InterPro

IPR012136. NADH_DH_b.
IPR004003. NADP_transhyd_b.
[Graphical view]

Pfam

PF02233. PNTB. 1 hit.
[Graphical view]

PIRSF

PIRSF000204. PNTB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PNTB_ECOLI

Accession

Primary (citable) accession number: P0AB67
Secondary accession number(s): P07002, P76890

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)