Skip Header

Contribute Send feedback
Read comments (?) or add your own

P0AB67 (PNTB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD(P) transhydrogenase subunit beta

EC=1.6.1.2
Alternative name(s):
Nicotinamide nucleotide transhydrogenase subunit beta
Pyridine nucleotide transhydrogenase subunit beta
Gene names
Name:pntB
Ordered Locus Names:b1602, JW1594
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.

Catalytic activity

NADPH + NAD+ = NADP+ + NADH.

Subunit structure

Heterodimer of an alpha and a beta chain.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the PNT beta subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 462462NAD(P) transhydrogenase subunit beta
PRO_0000199023

Regions

Topological domain1 – 33Periplasmic Potential
Transmembrane4 – 2421Helical; Potential
Topological domain25 – 4521Cytoplasmic Potential
Transmembrane46 – 6621Helical; Potential
Topological domain67 – 8216Periplasmic Potential
Transmembrane83 – 10321Helical; Potential
Topological domain104 – 11512Cytoplasmic Potential
Transmembrane116 – 13621Helical; Potential
Topological domain137 – 16428Periplasmic Potential
Transmembrane165 – 18521Helical; Potential
Topological domain186 – 1883Cytoplasmic Potential
Transmembrane189 – 20921Helical; Potential
Topological domain210 – 2156Periplasmic Potential
Transmembrane216 – 23621Helical; Potential
Topological domain237 – 2393Cytoplasmic Potential
Transmembrane240 – 26021Helical; Potential
Topological domain261 – 30848Periplasmic Potential
Transmembrane309 – 32921Helical; Potential
Topological domain330 – 462133Cytoplasmic Potential

Secondary structure

..................................... 462
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AB67 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: D11747291D271A2F

FASTA46248,723
        10         20         30         40         50         60 
MSGGLVTAAY IVAAILFIFS LAGLSKHETS RQGNNFGIAG MAIALIATIF GPDTGNVGWI 

        70         80         90        100        110        120 
LLAMVIGGAI GIRLAKKVEM TEMPELVAIL HSFVGLAAVL VGFNSYLHHD AGMAPILVNI 

       130        140        150        160        170        180 
HLTEVFLGIF IGAVTFTGSV VAFGKLCGKI SSKPLMLPNR HKMNLAALVV SFLLLIVFVR 

       190        200        210        220        230        240 
TDSVGLQVLA LLIMTAIALV FGWHLVASIG GADMPVVVSM LNSYSGWAAA AAGFMLSNDL 

       250        260        270        280        290        300 
LIVTGALVGS SGAILSYIMC KAMNRSFISV IAGGFGTDGS STGDDQEVGE HREITAEETA 

       310        320        330        340        350        360 
ELLKNSHSVI ITPGYGMAVA QAQYPVAEIT EKLRARGINV RFGIHPVAGR LPGHMNVLLA 

       370        380        390        400        410        420 
EAKVPYDIVL EMDEINDDFA DTDTVLVIGA NDTVNPAAQD DPKSPIAGMP VLEVWKAQNV 

       430        440        450        460 
IVFKRSMNTG YAGVQNPLFF KENTHMLFGD AKASVDAILK AL 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli."
Clarke D.M., Loo T.W., Gillam S., Bragg P.D.
Eur. J. Biochem. 158:647-653(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A mutation at Gly314 of the beta subunit of the Escherichia coli pyridine nucleotide transhydrogenase abolishes activity and affects the NADP(H)-induced conformational change."
Ahmad S., Glavas N.A., Bragg P.D.
Eur. J. Biochem. 207:733-739(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Topological analysis of the pyridine nucleotide transhydrogenase of Escherichia coli using proteolytic enzymes."
Tong R.C., Glavas N.A., Bragg P.D.
Biochim. Biophys. Acta 1080:19-28(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 266-277 AND 364-373.
[7]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04195 Genomic DNA. Translation: CAB37090.1.
X66086 Genomic DNA. Translation: CAA46885.1.
U00096 Genomic DNA. Translation: AAC74674.1.
AP009048 Genomic DNA. Translation: BAA15336.1.
PIRDEECXB. S24381.
RefSeqNP_416119.1. NC_000913.2.
YP_489865.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2BRUNMR-C286-462[»]
ProteinModelPortalP0AB67.
SMRP0AB67. Positions 296-462.
ModBaseSearch...

Protein-protein interaction databases

STRING511145.b1602.

Protein family/group databases

TCDB3.D.2.1.1. proton-translocating transhydrogenase (PTH) family.

Proteomic databases

PaxDbP0AB67.
PRIDEP0AB67.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74674; AAC74674; b1602.
BAA15336; BAA15336; BAA15336.
GeneID12930131.
946144.
KEGGecj:Y75_p1578.
eco:b1602.
PATRIC32118506. VBIEscCol129921_1673.

Organism-specific databases

EchoBASEEB0738.
EcoGeneEG10745. pntB.

Phylogenomic databases

eggNOGCOG1282.
HOGENOMHOG000243958.
KOK00325.
OMAGNVGWII.
ProtClustDBPRK09444.

Enzyme and pathway databases

BioCycEcoCyc:PNTB-MONOMER.
ECOL316407:JW1594-MONOMER.
MetaCyc:PNTB-MONOMER.

Gene expression databases

GenevestigatorP0AB67.

Family and domain databases

InterProIPR012136. NADH_DH_b.
[Graphical view]
PfamPF02233. PNTB. 1 hit.
[Graphical view]
PIRSFPIRSF000204. PNTB. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0AB67.

Entry information

Entry namePNTB_ECOLI
AccessionPrimary (citable) accession number: P0AB67
Secondary accession number(s): P07002, P76890
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 8, 2005
Last modified: May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families