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Protein

Lipopolysaccharide assembly protein B

Gene

lapB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. May act by modulating the proteolytic activity of FtsH towards LpxC. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane.UniRule annotation2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi357IronUniRule annotation1 Publication1
Metal bindingi360IronUniRule annotation1 Publication1
Metal bindingi371IronUniRule annotation1 Publication1
Metal bindingi374IronUniRule annotation1 Publication1

GO - Molecular functioni

  • metal ion binding Source: EcoCyc

GO - Biological processi

  • lipopolysaccharide metabolic process Source: EcoCyc

Keywordsi

Biological processStress response
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12691-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide assembly protein B1 PublicationUniRule annotation
Alternative name(s):
Lipopolysaccharide regulatory proteinUniRule annotation
Gene namesi
Name:lapB1 PublicationUniRule annotation
Synonyms:yciM
Ordered Locus Names:b1280, JW1272
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12691 yciM

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 20HelicalUniRule annotation1 PublicationAdd BLAST20
Topological domaini21 – 389CytoplasmicUniRule annotation1 PublicationAdd BLAST369

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion leads to changes in cell morphology and to the formation of bulges that contain cytoplasmic material and cause cell lysis. Mutants exhibit increased sensitivity to rifampicin and novobiocin, and to a mixture of SDS and EDTA. Growth is severely affected by osmolarity and temperature (PubMed:24187084). Mutant accumulates precursor forms of LPS and exhibits elevated levels of LpxC (PubMed:24722986).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi184C → S: Does not affect metal binding; when associated with S-258. 1 Publication1
Mutagenesisi258C → S: Does not affect metal binding; when associated with S-184. 1 Publication1
Mutagenesisi357C → S: Lack of activity; when associated with S-360; S-371 and S-374. 1 Publication1
Mutagenesisi360C → S: Lack of activity; when associated with S-357; S-371 and S-374. 1 Publication1
Mutagenesisi371C → R: Lack of activity. 1 Publication1
Mutagenesisi371C → S: Lack of activity; when associated with S-357; S-360 and S-374. 1 Publication1
Mutagenesisi372P → L: Lack of activity. 1 Publication1
Mutagenesisi374C → S: Lack of activity; when associated with S-357; S-360 and S-371. 1 Publication1
Mutagenesisi378S → P: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000138301 – 389Lipopolysaccharide assembly protein BAdd BLAST389

Proteomic databases

PaxDbiP0AB58
PRIDEiP0AB58

Expressioni

Inductioni

Induced by heat shock, via RpoH.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4259400, 56 interactors
DIPiDIP-48161N
IntActiP0AB58, 1 interactor
STRINGi316385.ECDH10B_1397

Structurei

3D structure databases

ProteinModelPortaliP0AB58
SMRiP0AB58
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati35 – 68TPR 1UniRule annotationAdd BLAST34
Repeati69 – 102TPR 2UniRule annotationAdd BLAST34
Repeati107 – 140TPR 3UniRule annotationAdd BLAST34
Repeati142 – 174TPR 4UniRule annotationAdd BLAST33
Repeati180 – 213TPR 5UniRule annotationAdd BLAST34
Repeati214 – 247TPR 6UniRule annotationAdd BLAST34
Repeati249 – 282TPR 7UniRule annotationAdd BLAST34

Domaini

The membrane anchor N-terminal domain is required for activity. The iron-binding domain is required for protein stability and function.1 Publication

Sequence similaritiesi

Belongs to the LapB family.UniRule annotationCurated

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CFV Bacteria
COG2956 LUCA
HOGENOMiHOG000257806
InParanoidiP0AB58
KOiK19804
OMAiIEAVQND
PhylomeDBiP0AB58

Family and domain databases

Gene3Di1.25.40.10, 2 hits
HAMAPiMF_00994 LPS_assembly_LapB, 1 hit
InterProiView protein in InterPro
IPR030865 LapB
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
SMARTiView protein in SMART
SM00028 TPR, 5 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 6 hits
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

P0AB58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLELLFLLLP VAAAYGWYMG RRSAQQNKQD EANRLSRDYV AGVNFLLSNQ
60 70 80 90 100
QDKAVDLFLD MLKEDTGTVE AHLTLGNLFR SRGEVDRAIR IHQTLMESAS
110 120 130 140 150
LTYEQRLLAI QQLGRDYMAA GLYDRAEDMF NQLTDETDFR IGALQQLLQI
160 170 180 190 200
YQATSEWQKA IDVAERLVKL GKDKQRVEIA HFYCELALQH MASDDLDRAM
210 220 230 240 250
TLLKKGAAAD KNSARVSIMM GRVFMAKGEY AKAVESLQRV ISQDRELVSE
260 270 280 290 300
TLEMLQTCYQ QLGKTAEWAE FLQRAVEENT GADAELMLAD IIEARDGSEA
310 320 330 340 350
AQVYITRQLQ RHPTMRVFHK LMDYHLNEAE EGRAKESLMV LRDMVGEKVR
360 370 380
SKPRYRCQKC GFTAYTLYWH CPSCRAWSTI KPIRGLDGL
Length:389
Mass (Da):44,531
Last modified:October 11, 2005 - v1
Checksum:iF4D1B9E2A526BCC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74362.1
AP009048 Genomic DNA Translation: BAA14834.1
M23250 Genomic DNA No translation available.
PIRiC64876
RefSeqiNP_415796.1, NC_000913.3
WP_000891353.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74362; AAC74362; b1280
BAA14834; BAA14834; BAA14834
GeneIDi944858
KEGGiecj:JW1272
eco:b1280
PATRICifig|1411691.4.peg.1001

Similar proteinsi

Entry informationi

Entry nameiLAPB_ECOLI
AccessioniPrimary (citable) accession number: P0AB58
Secondary accession number(s): P45576, P76836
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health