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Protein

Multiple stress resistance protein BhsA

Gene

bhsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reduces the permeability of the outer membrane to copper. Seems to be involved in the regulation of biofilm formation. May decrease biofilm formation by repressing cell-cell interaction and cell surface interaction.2 Publications

GO - Biological processi

  • response to copper ion Source: EcoCyc
  • single-species biofilm formation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

Copper

Enzyme and pathway databases

BioCyciEcoCyc:G6570-MONOMER.
ECOL316407:JW1098-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Multiple stress resistance protein BhsA
Alternative name(s):
Copper-induced outer membrane component
Gene namesi
Name:bhsA
Synonyms:comC, ycfR
Ordered Locus Names:b1112, JW1098
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13436. bhsA.

Subcellular locationi

  • Cell outer membrane 1 Publication

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption of this gene increases copper sensitivity, induces stress response genes in biofilms, increases aggregation and cell surface hydrophobicity, and decreases indole synthesis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 8563Multiple stress resistance protein BhsAPRO_0000013825Add
BLAST

Proteomic databases

EPDiP0AB40.
PaxDbiP0AB40.
PRIDEiP0AB40.

Expressioni

Inductioni

Repressed by ComR. Induced by copper via release of ComR. Induced in biofilms and by several stress conditions.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4261348. 15 interactions.
DIPiDIP-48158N.
STRINGi511145.b1112.

Structurei

3D structure databases

ProteinModelPortaliP0AB40.
SMRiP0AB40. Positions 21-85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BhsA/McbA family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105RC8. Bacteria.
ENOG4111Y56. LUCA.
HOGENOMiHOG000117400.
KOiK12151.
OMAiPADQHKV.
OrthoDBiEOG64NB35.
PhylomeDBiP0AB40.

Family and domain databases

Gene3Di3.30.1660.10. 1 hit.
InterProiIPR025543. Dodecin-like.
IPR010854. DUF1471.
[Graphical view]
PfamiPF07338. DUF1471. 1 hit.
[Graphical view]
SUPFAMiSSF159871. SSF159871. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AB40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNVKTLIAA AILSSMSFAS FAAVEVQSTP EGQQKVGTIS ANAGTNLGSL
60 70 80
EEQLAQKADE MGAKSFRITS VTGPNTLHGT AVIYK
Length:85
Mass (Da):8,815
Last modified:October 11, 2005 - v1
Checksum:iBB5122FE73E72181
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74196.1.
AP009048 Genomic DNA. Translation: BAA35927.1.
PIRiE64855.
RefSeqiNP_415630.1. NC_000913.3.
WP_000800153.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74196; AAC74196; b1112.
BAA35927; BAA35927; BAA35927.
GeneIDi945492.
KEGGiecj:JW1098.
eco:b1112.
PATRICi32117467. VBIEscCol129921_1156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74196.1.
AP009048 Genomic DNA. Translation: BAA35927.1.
PIRiE64855.
RefSeqiNP_415630.1. NC_000913.3.
WP_000800153.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AB40.
SMRiP0AB40. Positions 21-85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261348. 15 interactions.
DIPiDIP-48158N.
STRINGi511145.b1112.

Proteomic databases

EPDiP0AB40.
PaxDbiP0AB40.
PRIDEiP0AB40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74196; AAC74196; b1112.
BAA35927; BAA35927; BAA35927.
GeneIDi945492.
KEGGiecj:JW1098.
eco:b1112.
PATRICi32117467. VBIEscCol129921_1156.

Organism-specific databases

EchoBASEiEB3210.
EcoGeneiEG13436. bhsA.

Phylogenomic databases

eggNOGiENOG4105RC8. Bacteria.
ENOG4111Y56. LUCA.
HOGENOMiHOG000117400.
KOiK12151.
OMAiPADQHKV.
OrthoDBiEOG64NB35.
PhylomeDBiP0AB40.

Enzyme and pathway databases

BioCyciEcoCyc:G6570-MONOMER.
ECOL316407:JW1098-MONOMER.

Miscellaneous databases

PROiP0AB40.

Family and domain databases

Gene3Di3.30.1660.10. 1 hit.
InterProiIPR025543. Dodecin-like.
IPR010854. DUF1471.
[Graphical view]
PfamiPF07338. DUF1471. 1 hit.
[Graphical view]
SUPFAMiSSF159871. SSF159871. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "YcfR (BhsA) influences Escherichia coli biofilm formation through stress response and surface hydrophobicity."
    Zhang X.-S., Garcia-Contreras R., Wood T.K.
    J. Bacteriol. 189:3051-3062(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN REGULATION OF BIOFILM FORMATION, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / BW25113.
  5. "The copper-inducible ComR (YcfQ) repressor regulates expression of ComC (YcfR), which affects copper permeability of the outer membrane of Escherichia coli."
    Mermod M., Magnani D., Solioz M., Stoyanov J.V.
    BioMetals 25:33-43(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN COPPER TOLERANCE, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiBHSA_ECOLI
AccessioniPrimary (citable) accession number: P0AB40
Secondary accession number(s): P75953
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 16, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.