P0AB40 (BHSA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multiple stress resistance protein BhsA

Alternative name(s):
Copper-induced outer membrane component

Gene names

Name:	bhsA
Synonyms:	comC, ycfR
Ordered Locus Names:	b1112, JW1098

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reduces the permeability of the outer membrane to copper. Seems to be involved in the regulation of biofilm formation. May decrease biofilm formation by repressing cell-cell interaction and cell surface interaction. Ref.4 Ref.5
Subcellular location	Cell outer membrane Ref.5.
Induction	Repressed by ComR. Induced by copper via release of ComR. Induced in biofilms and by several stress conditions. Ref.4 Ref.5
Disruption phenotype	Disruption of this gene increases copper sensitivity, induces stress response genes in biofilms, increases aggregation and cell surface hydrophobicity, and decreases indole synthesis. Ref.4 Ref.5
Sequence similarities	Belongs to the BhsA/McbA family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
Ligand	Copper
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biofilm formation Inferred from mutant phenotype Ref.4. Source: EcoCyc response to copper ion Inferred from mutant phenotype Ref.5. Source: EcoCyc response to stress Inferred from mutant phenotype Ref.4. Source: EcoCyc
Cellular_component	cell outer membrane Inferred from direct assay Ref.5. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 85	63	Multiple stress resistance protein BhsA		PRO_0000013825

Sequences

	Sequence		Length	Mass (Da)
	P0AB40 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: BB5122FE73E72181	FASTA	85	8,815
	10 20 30 40 50 60 MKNVKTLIAA AILSSMSFAS FAAVEVQSTP EGQQKVGTIS ANAGTNLGSL EEQLAQKADE 70 80 MGAKSFRITS VTGPNTLHGT AVIYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"YcfR (BhsA) influences Escherichia coli biofilm formation through stress response and surface hydrophobicity." Zhang X.-S., Garcia-Contreras R., Wood T.K. J. Bacteriol. 189:3051-3062(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN REGULATION OF BIOFILM FORMATION, INDUCTION, DISRUPTION PHENOTYPE. Strain: K12 / BW25113.
[5]	"The copper-inducible ComR (YcfQ) repressor regulates expression of ComC (YcfR), which affects copper permeability of the outer membrane of Escherichia coli." Mermod M., Magnani D., Solioz M., Stoyanov J.V. BioMetals 25:33-43(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN COPPER TOLERANCE, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74196.1. AP009048 Genomic DNA. Translation: BAA35927.1.
PIR	E64855.
RefSeq	NP_415630.1. NC_000913.2. YP_489380.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AB40.
SMR	P0AB40. Positions 1-85.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48158N.
STRING	511145.b1112.
Proteomic databases
PaxDb	P0AB40.
PRIDE	P0AB40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74196; AAC74196; b1112. BAA35927; BAA35927; BAA35927.
GeneID	12932354. 945492.
KEGG	ecj:Y75_p1082. eco:b1112.
PATRIC	32117467. VBIEscCol129921_1156.
Organism-specific databases
EchoBASE	EB3210.
EcoGene	EG13436. bhsA.
Phylogenomic databases
eggNOG	NOG12952.
HOGENOM	HOG000117400.
KO	K12151.
OMA	ATGNNLM.
ProtClustDB	CLSK879955.
Enzyme and pathway databases
BioCyc	EcoCyc:G6570-MONOMER. ECOL316407:JW1098-MONOMER.
Gene expression databases
Genevestigator	P0AB40.
Family and domain databases
Gene3D	3.30.1660.10. 1 hit.
InterPro	IPR025543. Dodecin-like. IPR010854. DUF1471. [Graphical view]
Pfam	PF07338. DUF1471. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BHSA_ECOLI

Accession

Primary (citable) accession number: P0AB40
Secondary accession number(s): P75953

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program