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Protein

Penicillin-binding protein activator LpoB

Gene

lpoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of peptidoglycan synthesis that is essential for the function of penicillin-binding protein 1B (PBP1b). Stimulates transpeptidase and transglycosylase activities of PBP1b in vitro. May also contribute to outer membrane constriction during cell division, in complex with PBP1b.2 Publications

GO - Molecular functioni

  • enzyme activator activity Source: EcoCyc
  • enzyme binding Source: EcoCyc
  • enzyme regulator activity Source: UniProtKB

GO - Biological processi

  • peptidoglycan biosynthetic process Source: UniProtKB
  • positive regulation of catalytic activity Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell shape, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G6565-MONOMER.
ECOL316407:JW5157-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein activator LpoB
Short name:
PBP activator LpoB
Alternative name(s):
Lipoprotein activator of PBP from the outer membrane B
Gene namesi
Name:lpoB
Synonyms:ycfM
Ordered Locus Names:b1105, JW5157
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13431. lpoB.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • periplasmic side of cell outer membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant shows sensitivity to many beta-lactams. Absence of both LpoA and LpoB leads to a decrease in peptide cross-linking and to cell lysis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 213194Penicillin-binding protein activator LpoBPRO_0000168837Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi20 – 201N-palmitoyl cysteineSequence analysis
Lipidationi20 – 201S-diacylglycerol cysteineSequence analysis

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP0AB38.
PaxDbiP0AB38.
PRIDEiP0AB38.

Interactioni

Subunit structurei

Interacts with PBP1b.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mrcBP029194EBI-3405489,EBI-909769

GO - Molecular functioni

  • enzyme binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4263027. 150 interactions.
IntActiP0AB38. 9 interactions.
STRINGi511145.b1105.

Structurei

3D structure databases

ProteinModelPortaliP0AB38.
SMRiP0AB38. Positions 21-213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpoB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108WEF. Bacteria.
COG3417. LUCA.
HOGENOMiHOG000119084.
KOiK07337.
OMAiEIVWSGN.
OrthoDBiEOG6SJJMG.

Family and domain databases

HAMAPiMF_01889. LpoB.
InterProiIPR014094. LpoB.
[Graphical view]
PfamiPF13036. LpoB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02722. lp_. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AB38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKMSRYALI TALAMFLAGC VGQREPAPVE EVKPAPEQPA EPQQPVPTVP
60 70 80 90 100
SVPTIPQQPG PIEHEDQTAP PAPHIRHYDW NGAMQPMVSK MLGADGVTAG
110 120 130 140 150
SVLLVDSVNN RTNGSLNAAE ATETLRNALA NNGKFTLVSA QQLSMAKQQL
160 170 180 190 200
GLSPQDSLGT RSKAIGIARN VGAHYVLYSS ASGNVNAPTL QMQLMLVQTG
210
EIIWSGKGAV SQQ
Length:213
Mass (Da):22,516
Last modified:October 11, 2005 - v1
Checksum:i06B529F3F4722AD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74189.1.
AP009048 Genomic DNA. Translation: BAA35912.2.
PIRiF64854.
RefSeqiNP_415623.1. NC_000913.3.
WP_000164439.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74189; AAC74189; b1105.
BAA35912; BAA35912; BAA35912.
GeneIDi948536.
KEGGiecj:JW5157.
eco:b1105.
PATRICi32117453. VBIEscCol129921_1149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74189.1.
AP009048 Genomic DNA. Translation: BAA35912.2.
PIRiF64854.
RefSeqiNP_415623.1. NC_000913.3.
WP_000164439.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AB38.
SMRiP0AB38. Positions 21-213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263027. 150 interactions.
IntActiP0AB38. 9 interactions.
STRINGi511145.b1105.

Proteomic databases

EPDiP0AB38.
PaxDbiP0AB38.
PRIDEiP0AB38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74189; AAC74189; b1105.
BAA35912; BAA35912; BAA35912.
GeneIDi948536.
KEGGiecj:JW5157.
eco:b1105.
PATRICi32117453. VBIEscCol129921_1149.

Organism-specific databases

EchoBASEiEB3205.
EcoGeneiEG13431. lpoB.

Phylogenomic databases

eggNOGiENOG4108WEF. Bacteria.
COG3417. LUCA.
HOGENOMiHOG000119084.
KOiK07337.
OMAiEIVWSGN.
OrthoDBiEOG6SJJMG.

Enzyme and pathway databases

BioCyciEcoCyc:G6565-MONOMER.
ECOL316407:JW5157-MONOMER.

Miscellaneous databases

PROiP0AB38.

Family and domain databases

HAMAPiMF_01889. LpoB.
InterProiIPR014094. LpoB.
[Graphical view]
PfamiPF13036. LpoB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02722. lp_. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: FUNCTION, INTERACTION WITH PBP1B, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: K12.
  5. "Lipoprotein cofactors located in the outer membrane activate bacterial cell wall polymerases."
    Paradis-Bleau C., Markovski M., Uehara T., Lupoli T.J., Walker S., Kahne D.E., Bernhardt T.G.
    Cell 143:1110-1120(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PBP1B, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiLPOB_ECOLI
AccessioniPrimary (citable) accession number: P0AB38
Secondary accession number(s): P75947, Q9R424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 16, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.