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Protein

Sulfurtransferase TusE

Gene

tusE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions. Could accept sulfur from TusD.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei108Cysteine persulfide intermediate1

GO - Molecular functioni

  • sulfur carrier activity Source: EcoCyc
  • transferase activity Source: UniProtKB-KW

GO - Biological processi

  • tRNA wobble position uridine thiolation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG12876-MONOMER.
ECOL316407:JW0952-MONOMER.
MetaCyc:EG12876-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfurtransferase TusE (EC:2.8.1.-)
Alternative name(s):
tRNA 2-thiouridine synthesizing protein E
Gene namesi
Name:tusE
Synonyms:yccK
Ordered Locus Names:b0969, JW0952
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12876. tusE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi108C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001687891 – 109Sulfurtransferase TusEAdd BLAST109

Proteomic databases

PaxDbiP0AB18.
PRIDEiP0AB18.

Interactioni

Subunit structurei

Interacts with the TusBCD complex. Interacts with MnmA.1 Publication

Protein-protein interaction databases

BioGridi4260042. 10 interactors.
DIPiDIP-48154N.
IntActiP0AB18. 11 interactors.
STRINGi511145.b0969.

Structurei

3D structure databases

ProteinModelPortaliP0AB18.
SMRiP0AB18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrC/TusE family.Curated

Phylogenomic databases

eggNOGiENOG4108Z0T. Bacteria.
COG2920. LUCA.
HOGENOMiHOG000279490.
InParanoidiP0AB18.
KOiK11179.
OMAiEYNTSPA.
PhylomeDBiP0AB18.

Family and domain databases

InterProiIPR025526. DsrC-like_dom.
IPR007453. DsrC/TusE.
[Graphical view]
PfamiPF04358. DsrC. 1 hit.
[Graphical view]
PIRSFiPIRSF006223. DsrC_TusE. 1 hit.
SUPFAMiSSF69721. SSF69721. 1 hit.
TIGRFAMsiTIGR03342. dsrC_tusE_dsvC. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AB18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIFEGKEIE TDTEGYLKES SQWSEPLAVV IAENEGISLS PEHWEVVRFV
60 70 80 90 100
RDFYLEFNTS PAIRMLVKAM ANKFGEEKGN SRYLYRLFPK GPAKQATKIA

GLPKPVKCI
Length:109
Mass (Da):12,410
Last modified:October 11, 2005 - v1
Checksum:iFDB0CFF89114EBE7
GO

Sequence cautioni

The sequence X00547 differs from that shown. Reason: Frameshift at position 24.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74055.2.
AP009048 Genomic DNA. Translation: BAA35734.1.
X00547 Genomic DNA. No translation available.
PIRiH64837.
RefSeqiNP_415489.4. NC_000913.3.
WP_000904442.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74055; AAC74055; b0969.
BAA35734; BAA35734; BAA35734.
GeneIDi945023.
KEGGiecj:JW0952.
eco:b0969.
PATRICi32117159. VBIEscCol129921_1004.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74055.2.
AP009048 Genomic DNA. Translation: BAA35734.1.
X00547 Genomic DNA. No translation available.
PIRiH64837.
RefSeqiNP_415489.4. NC_000913.3.
WP_000904442.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AB18.
SMRiP0AB18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260042. 10 interactors.
DIPiDIP-48154N.
IntActiP0AB18. 11 interactors.
STRINGi511145.b0969.

Proteomic databases

PaxDbiP0AB18.
PRIDEiP0AB18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74055; AAC74055; b0969.
BAA35734; BAA35734; BAA35734.
GeneIDi945023.
KEGGiecj:JW0952.
eco:b0969.
PATRICi32117159. VBIEscCol129921_1004.

Organism-specific databases

EchoBASEiEB2714.
EcoGeneiEG12876. tusE.

Phylogenomic databases

eggNOGiENOG4108Z0T. Bacteria.
COG2920. LUCA.
HOGENOMiHOG000279490.
InParanoidiP0AB18.
KOiK11179.
OMAiEYNTSPA.
PhylomeDBiP0AB18.

Enzyme and pathway databases

BioCyciEcoCyc:EG12876-MONOMER.
ECOL316407:JW0952-MONOMER.
MetaCyc:EG12876-MONOMER.

Miscellaneous databases

PROiP0AB18.

Family and domain databases

InterProiIPR025526. DsrC-like_dom.
IPR007453. DsrC/TusE.
[Graphical view]
PfamiPF04358. DsrC. 1 hit.
[Graphical view]
PIRSFiPIRSF006223. DsrC_TusE. 1 hit.
SUPFAMiSSF69721. SSF69721. 1 hit.
TIGRFAMsiTIGR03342. dsrC_tusE_dsvC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTUSE_ECOLI
AccessioniPrimary (citable) accession number: P0AB18
Secondary accession number(s): P45572, P75878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.