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Protein

Replication-associated recombination protein A

Gene

rarA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase that plays important roles in cellular responses to stalled DNA replication processes.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi57 – 64ATP1 Publication8

GO - Molecular functioni

GO - Biological processi

  • DNA-dependent DNA replication Source: EcoCyc
  • DNA recombination Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12690-MONOMER.
ECOL316407:JW0875-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated recombination protein A1 Publication
Gene namesi
Name:rarA1 Publication
Synonyms:mgsA1 Publication, ycaJ
Ordered Locus Names:b0892, JW0875
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12690. rarA.

Subcellular locationi

GO - Cellular componenti

  • replisome Source: EcoliWiki
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mutation gives no obvious mutant phenotype, but, when combined with xer or dif mutants, leads to slower growth.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi156R → A: Lack of ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001687551 – 447Replication-associated recombination protein AAdd BLAST447

Proteomic databases

PaxDbiP0AAZ4.
PRIDEiP0AAZ4.

Interactioni

Subunit structurei

Homotetramer. Interacts with the single-stranded DNA-binding protein (ssb).1 Publication

Protein-protein interaction databases

BioGridi4259357. 22 interactors.
DIPiDIP-48151N.
IntActiP0AAZ4. 7 interactors.
MINTiMINT-1224512.
STRINGi511145.b0892.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PVSX-ray2.50A/B/C/D1-447[»]
ProteinModelPortaliP0AAZ4.
SMRiP0AAZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Contains an N-terminal ATP-binding domain, an adjacent helical lid domain, and a C-terminal tetramerization domain.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D1H. Bacteria.
COG2256. LUCA.
HOGENOMiHOG000017623.
InParanoidiP0AAZ4.
KOiK07478.
OMAiQHYNLIS.
PhylomeDBiP0AAZ4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR032423. AAA_assoc_2.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR021886. MgsA_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16193. AAA_assoc_2. 1 hit.
PF12002. MgsA_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AAZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLSLDFSD NTFQPLAARM RPENLAQYIG QQHLLAAGKP LPRAIEAGHL
60 70 80 90 100
HSMILWGPPG TGKTTLAEVI ARYANADVER ISAVTSGVKE IREAIERARQ
110 120 130 140 150
NRNAGRRTIL FVDEVHRFNK SQQDAFLPHI EDGTITFIGA TTENPSFELN
160 170 180 190 200
SALLSRARVY LLKSLSTEDI EQVLTQAMED KTRGYGGQDI VLPDETRRAI
210 220 230 240 250
AELVNGDARR ALNTLEMMAD MAEVDDSGKR VLKPELLTEI AGERSARFDN
260 270 280 290 300
KGDRFYDLIS ALHKSVRGSA PDAALYWYAR IITAGGDPLY VARRCLAIAS
310 320 330 340 350
EDVGNADPRA MQVAIAAWDC FTRVGPAEGE RAIAQAIVYL ACAPKSNAVY
360 370 380 390 400
TAFKAALADA RERPDYDVPV HLRNAPTKLM KEMGYGQEYR YAHDEANAYA
410 420 430 440
AGEVYFPPEI AQTRYYFPTN RGLEGKIGEK LAWLAEQDQN SPIKRYR
Length:447
Mass (Da):49,626
Last modified:October 11, 2005 - v1
Checksum:i116ACBE38E4E4A3A
GO

Sequence cautioni

The sequence X05017 differs from that shown. Reason: Frameshift at position 370.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73978.1.
AP009048 Genomic DNA. Translation: BAA35617.1.
D49398 Genomic DNA. No translation available.
X05017 Genomic DNA. No translation available.
PIRiC64828.
RefSeqiNP_415412.1. NC_000913.3.
WP_000067755.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73978; AAC73978; b0892.
BAA35617; BAA35617; BAA35617.
GeneIDi945505.
KEGGiecj:JW0875.
eco:b0892.
PATRICi32116995. VBIEscCol129921_0922.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73978.1.
AP009048 Genomic DNA. Translation: BAA35617.1.
D49398 Genomic DNA. No translation available.
X05017 Genomic DNA. No translation available.
PIRiC64828.
RefSeqiNP_415412.1. NC_000913.3.
WP_000067755.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PVSX-ray2.50A/B/C/D1-447[»]
ProteinModelPortaliP0AAZ4.
SMRiP0AAZ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259357. 22 interactors.
DIPiDIP-48151N.
IntActiP0AAZ4. 7 interactors.
MINTiMINT-1224512.
STRINGi511145.b0892.

Proteomic databases

PaxDbiP0AAZ4.
PRIDEiP0AAZ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73978; AAC73978; b0892.
BAA35617; BAA35617; BAA35617.
GeneIDi945505.
KEGGiecj:JW0875.
eco:b0892.
PATRICi32116995. VBIEscCol129921_0922.

Organism-specific databases

EchoBASEiEB2553.
EcoGeneiEG12690. rarA.

Phylogenomic databases

eggNOGiENOG4105D1H. Bacteria.
COG2256. LUCA.
HOGENOMiHOG000017623.
InParanoidiP0AAZ4.
KOiK07478.
OMAiQHYNLIS.
PhylomeDBiP0AAZ4.

Enzyme and pathway databases

BioCyciEcoCyc:EG12690-MONOMER.
ECOL316407:JW0875-MONOMER.

Miscellaneous databases

PROiP0AAZ4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR032423. AAA_assoc_2.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR021886. MgsA_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF16193. AAA_assoc_2. 1 hit.
PF12002. MgsA_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRARA_ECOLI
AccessioniPrimary (citable) accession number: P0AAZ4
Secondary accession number(s): P45526, P75833
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression causes an increase in mutation frequency and inhibits growth of recA mutant.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.