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Protein

Replication-associated recombination protein A

Gene

rarA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent ATPase that plays important roles in cellular responses to stalled DNA replication processes.3 Publications

Miscellaneous

Overexpression causes an increase in mutation frequency and inhibits growth of recA mutant.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi57 – 64ATP1 Publication8

GO - Molecular functioni

GO - Biological processi

  • DNA-dependent DNA replication Source: EcoCyc
  • DNA recombination Source: EcoCyc

Keywordsi

Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12690-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated recombination protein A1 Publication
Gene namesi
Name:rarA1 Publication
Synonyms:mgsA1 Publication, ycaJ
Ordered Locus Names:b0892, JW0875
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12690 rarA

Subcellular locationi

GO - Cellular componenti

  • replisome Source: EcoliWiki

Pathology & Biotechi

Disruption phenotypei

Mutation gives no obvious mutant phenotype, but, when combined with xer or dif mutants, leads to slower growth.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi156R → A: Lack of ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001687551 – 447Replication-associated recombination protein AAdd BLAST447

Proteomic databases

PaxDbiP0AAZ4
PRIDEiP0AAZ4

Interactioni

Subunit structurei

Homotetramer. Interacts with the single-stranded DNA-binding protein (ssb).1 Publication

Protein-protein interaction databases

BioGridi4259357, 64 interactors
DIPiDIP-48151N
IntActiP0AAZ4, 7 interactors
STRINGi316385.ECDH10B_0962

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PVSX-ray2.50A/B/C/D1-447[»]
ProteinModelPortaliP0AAZ4
SMRiP0AAZ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Contains an N-terminal ATP-binding domain, an adjacent helical lid domain, and a C-terminal tetramerization domain.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D1H Bacteria
COG2256 LUCA
HOGENOMiHOG000017623
InParanoidiP0AAZ4
KOiK07478
OMAiRIILSQC
PhylomeDBiP0AAZ4

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR032423 AAA_assoc_2
IPR003959 ATPase_AAA_core
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR021886 MgsA_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF16193 AAA_assoc_2, 1 hit
PF12002 MgsA_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

P0AAZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLSLDFSD NTFQPLAARM RPENLAQYIG QQHLLAAGKP LPRAIEAGHL
60 70 80 90 100
HSMILWGPPG TGKTTLAEVI ARYANADVER ISAVTSGVKE IREAIERARQ
110 120 130 140 150
NRNAGRRTIL FVDEVHRFNK SQQDAFLPHI EDGTITFIGA TTENPSFELN
160 170 180 190 200
SALLSRARVY LLKSLSTEDI EQVLTQAMED KTRGYGGQDI VLPDETRRAI
210 220 230 240 250
AELVNGDARR ALNTLEMMAD MAEVDDSGKR VLKPELLTEI AGERSARFDN
260 270 280 290 300
KGDRFYDLIS ALHKSVRGSA PDAALYWYAR IITAGGDPLY VARRCLAIAS
310 320 330 340 350
EDVGNADPRA MQVAIAAWDC FTRVGPAEGE RAIAQAIVYL ACAPKSNAVY
360 370 380 390 400
TAFKAALADA RERPDYDVPV HLRNAPTKLM KEMGYGQEYR YAHDEANAYA
410 420 430 440
AGEVYFPPEI AQTRYYFPTN RGLEGKIGEK LAWLAEQDQN SPIKRYR
Length:447
Mass (Da):49,626
Last modified:October 11, 2005 - v1
Checksum:i116ACBE38E4E4A3A
GO

Sequence cautioni

The sequence X05017 differs from that shown. Reason: Frameshift at position 370.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73978.1
AP009048 Genomic DNA Translation: BAA35617.1
D49398 Genomic DNA No translation available.
X05017 Genomic DNA No translation available.
PIRiC64828
RefSeqiNP_415412.1, NC_000913.3
WP_000067755.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73978; AAC73978; b0892
BAA35617; BAA35617; BAA35617
GeneIDi945505
KEGGiecj:JW0875
eco:b0892
PATRICifig|1411691.4.peg.1385

Entry informationi

Entry nameiRARA_ECOLI
AccessioniPrimary (citable) accession number: P0AAZ4
Secondary accession number(s): P45526, P75833
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health