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Protein

Multidrug efflux pump accessory protein AcrZ

Gene

acrZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

AcrA-AcrB-AcrZ-TolC is a drug efflux protein complex with a broad substrate specificity. This protein binds to AcrB and is required for efflux of some but not all substrates, suggesting it may influence the specificity of drug export.UniRule annotation1 Publication

GO - Molecular functioni

  • drug transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  • cellular response to cell envelope stress Source: EcoCyc
  • response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Stress response, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6396-MONOMER.
ECOL316407:JW5102-MONOMER.

Protein family/group databases

TCDBi2.A.6.2.2. the resistance-nodulation-cell division (rnd) superfamily.
8.A.50.1.1. the acrz rnd auxillary subunit (acrz) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug efflux pump accessory protein AcrZUniRule annotation
Alternative name(s):
AcrAB-TolC multidrug efflux pump accessory protein AcrZUniRule annotation
Acridine resistance protein ZUniRule annotation
Gene namesi
Name:acrZUniRule annotation
Synonyms:ybhT
Ordered Locus Names:b0762, JW5102
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14328. acrZ.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7PeriplasmicCurated7
Transmembranei8 – 28HelicalUniRule annotationAdd BLAST21
Topological domaini29 – 49CytoplasmicCuratedAdd BLAST21

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Becomes sensitive to some antibiotics (chloramphenicol, puromycin and tetracycline) but not to others (erythromycin, rifampicin and fusidic acid).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30G → R: Loss of chloramphenicol resistance, does not bind AcrB (dominant-negative to wild-type). Partially suppressed by AcrB H526Y. 1 Publication1
Mutagenesisi46 – 49Missing : Loss of chloramphenicol resistance, still binds AcrB (dominant-negative to wild-type). 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000138111 – 49Multidrug efflux pump accessory protein AcrZAdd BLAST49

Proteomic databases

PaxDbiP0AAW9.
PRIDEiP0AAW9.

Expressioni

Inductioni

Constitutively expressed (PubMed:19121005), may be repressed at 45 degrees Celsius (PubMed:19121005) (at protein level). Positively regulated by MarA, Rob and SoxS transcriptional regulators.3 Publications

Interactioni

Subunit structurei

Part of the AcrA-AcrB-AcrZ-TolC efflux pump, interacts directly with AcrB.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
acrBP312244EBI-6313593,EBI-551006

Protein-protein interaction databases

BioGridi4259947. 12 interactors.
DIPiDIP-11423N.
IntActiP0AAW9. 2 interactors.
STRINGi511145.b0762.

Structurei

Secondary structure

149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 35Combined sources34

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C48X-ray3.30C1-49[»]
ProteinModelPortaliP0AAW9.
SMRiP0AAW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AcrZ family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000219534.

Family and domain databases

HAMAPiMF_01484. AcrZ. 1 hit.
InterProiIPR019702. AcrZ.
[Graphical view]
PfamiPF10766. AcrZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
MLELLKSLVF AVIMVPVVMA IILGLIYGLG EVFNIFSGVG KKDQPGQNH
Length:49
Mass (Da):5,300
Last modified:October 11, 2005 - v1
Checksum:i1B1185D27F1F37F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73849.1.
AP009048 Genomic DNA. Translation: BAA35426.2.
PIRiB64812.
RefSeqiNP_415283.1. NC_000913.3.
WP_000891515.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73849; AAC73849; b0762.
BAA35426; BAA35426; BAA35426.
GeneIDi945365.
KEGGiecj:JW5102.
eco:b0762.
PATRICi32116725. VBIEscCol129921_0788.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73849.1.
AP009048 Genomic DNA. Translation: BAA35426.2.
PIRiB64812.
RefSeqiNP_415283.1. NC_000913.3.
WP_000891515.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C48X-ray3.30C1-49[»]
ProteinModelPortaliP0AAW9.
SMRiP0AAW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259947. 12 interactors.
DIPiDIP-11423N.
IntActiP0AAW9. 2 interactors.
STRINGi511145.b0762.

Protein family/group databases

TCDBi2.A.6.2.2. the resistance-nodulation-cell division (rnd) superfamily.
8.A.50.1.1. the acrz rnd auxillary subunit (acrz) family.

Proteomic databases

PaxDbiP0AAW9.
PRIDEiP0AAW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73849; AAC73849; b0762.
BAA35426; BAA35426; BAA35426.
GeneIDi945365.
KEGGiecj:JW5102.
eco:b0762.
PATRICi32116725. VBIEscCol129921_0788.

Organism-specific databases

EchoBASEiEB4074.
EcoGeneiEG14328. acrZ.

Phylogenomic databases

HOGENOMiHOG000219534.

Enzyme and pathway databases

BioCyciEcoCyc:G6396-MONOMER.
ECOL316407:JW5102-MONOMER.

Miscellaneous databases

PROiP0AAW9.

Family and domain databases

HAMAPiMF_01484. AcrZ. 1 hit.
InterProiIPR019702. AcrZ.
[Graphical view]
PfamiPF10766. AcrZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACRZ_ECOLI
AccessioniPrimary (citable) accession number: P0AAW9
Secondary accession number(s): P75759
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.