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Protein

Ribosomal silencing factor RsfS

Gene

rsfS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a ribosomal silencing factor. Addition to isolated ribosomal subunits partially inhibits their association, preventing translation. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8, preventing association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.1 Publication

GO - Molecular functioni

  • ribosomal large subunit binding Source: EcoCyc

GO - Biological processi

  • negative regulation of ribosome biogenesis Source: UniProtKB
  • negative regulation of translation Source: UniProtKB

Keywordsi

Molecular functionRepressor
Biological processTranslation regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG11255-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal silencing factor RsfS
Gene namesi
Name:rsfS
Synonyms:rsfA, slm3, ybeB
Ordered Locus Names:b0637, JW5090
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11255 rsfS

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cell viability is dramatically reduced in stationary phase, cells have a 10 hour growth block upon transition from rich to poor medium. Protein translation in stationary phase is derepressed.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001686741 – 105Ribosomal silencing factor RsfSAdd BLAST105

Proteomic databases

PaxDbiP0AAT6
PRIDEiP0AAT6

Interactioni

Subunit structurei

Interacts with ribosomal protein L14 (rplN).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
yehLP333483EBI-560192,EBI-489750

Protein-protein interaction databases

BioGridi4261658, 43 interactors
DIPiDIP-48141N
IntActiP0AAT6, 10 interactors
STRINGi316385.ECDH10B_0706

Structurei

3D structure databases

ProteinModelPortaliP0AAT6
SMRiP0AAT6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Iojap/RsfS family.Curated

Phylogenomic databases

eggNOGiENOG4105KEM Bacteria
COG0799 LUCA
HOGENOMiHOG000016051
InParanoidiP0AAT6
KOiK09710
OMAiVVHVFQK
PhylomeDBiP0AAT6

Family and domain databases

HAMAPiMF_01477 Iojap_RsfS, 1 hit
InterProiView protein in InterPro
IPR004394 Iojap/RsfS/C7orf30
PANTHERiPTHR21043 PTHR21043, 1 hit
TIGRFAMsiTIGR00090 rsfS_iojap_ybeB, 1 hit

Sequencei

Sequence statusi: Complete.

P0AAT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGKALQDFV IDKIDDLKGQ DIIALDVQGK SSITDCMIIC TGTSSRHVMS
60 70 80 90 100
IADHVVQESR AAGLLPLGVE GENSADWIVV DLGDVIVHVM QEESRRLYEL

EKLWS
Length:105
Mass (Da):11,582
Last modified:October 11, 2005 - v1
Checksum:i7C1315607BD0B610
GO

Sequence cautioni

The sequence CAA28199 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04516 Genomic DNA Translation: CAA28199.1 Different initiation.
U82598 Genomic DNA Translation: AAB40837.1
U00096 Genomic DNA Translation: AAC73738.2
AP009048 Genomic DNA Translation: BAA35284.2
RefSeqiNP_415170.4, NC_000913.3
WP_001161664.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73738; AAC73738; b0637
BAA35284; BAA35284; BAA35284
GeneIDi945237
KEGGiecj:JW5090
eco:b0637
PATRICifig|1411691.4.peg.1631

Similar proteinsi

Entry informationi

Entry nameiIOJAP_ECOLI
AccessioniPrimary (citable) accession number: P0AAT6
Secondary accession number(s): P05848, P77107
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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