P0AAT6 (IOJAP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal silencing factor RsfS

Gene names

Name:	rsfS
Synonyms:	rsfA, slm3, ybeB
Ordered Locus Names:	b0637, JW5090

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a ribosomal silencing factor. Addition to isolated ribosomal subunits partially inhibits their association, preventing translation. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8, preventing association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation. Ref.7
Subunit structure	Interacts with ribosomal protein L14 (rplN). Ref.6 Ref.7
Subcellular location	Cytoplasm. Note: Comigrates with the 50S ribosomal subunit in sucrose gradients, specifically with ribosomal protein L14 (rplN). Ref.6
Disruption phenotype	Cell viability is dramatically reduced in stationary phase, cells have a 10 hour growth block upon transition from rich to poor medium. Protein translation in stationary phase is derepressed. Ref.7
Sequence similarities	Belongs to the Iojap/RsfS family.
Sequence caution	The sequence CAA28199.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Translation regulation
Cellular component	Cytoplasm
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of ribosome biogenesis Inferred from direct assay Ref.7. Source: UniProtKB negative regulation of translation Inferred from direct assay Ref.7. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ribosomal large subunit binding Inferred from direct assay Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Ribosomal silencing factor RsfS HAMAP-Rule MF_01477		PRO_0000168674

Sequences

	Sequence		Length	Mass (Da)
	P0AAT6 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 7C1315607BD0B610	FASTA	105	11,582
	10 20 30 40 50 60 MQGKALQDFV IDKIDDLKGQ DIIALDVQGK SSITDCMIIC TGTSSRHVMS IADHVVQESR 70 80 90 100 AAGLLPLGVE GENSADWIVV DLGDVIVHVM QEESRRLYEL EKLWS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12." Asoh S., Matsuzawa H., Ishino F., Strominger J.L., Matsuhashi M., Ohta T. Eur. J. Biochem. 160:231-238(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The Escherichia coli GTPase CgtAE is involved in late steps of large ribosome assembly." Jiang M., Datta K., Walker A., Strahler J., Bagamasbad P., Andrews P.C., Maddock J.R. J. Bacteriol. 188:6757-6770(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, ASSOCIATION WITH THE 50S RIBOSOMAL SUBUNIT. Strain: K12 / MG1655 / ATCC 47076.
[7]	"RsfA (YbeB) proteins are conserved ribosomal silencing factors." Hauser R., Pech M., Kijek J., Yamamoto H., Titz B., Naeve F., Tovchigrechko A., Yamamoto K., Szaflarski W., Takeuchi N., Stellberger T., Diefenbacher M.E., Nierhaus K.H., Uetz P. PLoS Genet. 8:E1002815-E1002815(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RIBOSOMAL PROTEIN L14 (RPLN), DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04516 Genomic DNA. Translation: CAA28199.1. Different initiation. U82598 Genomic DNA. Translation: AAB40837.1. U00096 Genomic DNA. Translation: AAC73738.2. AP009048 Genomic DNA. Translation: BAA35284.2.
RefSeq	NP_415170.4. NC_000913.2. YP_488928.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AAT6.
SMR	P0AAT6. Positions 1-104.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48141N.
IntAct	P0AAT6. 10 interactions.
MINT	MINT-1226369.
STRING	511145.b0637.
Proteomic databases
PaxDb	P0AAT6.
PRIDE	P0AAT6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73738; AAC73738; b0637. BAA35284; BAA35284; BAA35284.
GeneID	12932664. 945237.
KEGG	ecj:Y75_p0627. eco:b0637.
PATRIC	32116457. VBIEscCol129921_0668.
Organism-specific databases
EchoBASE	EB1235.
EcoGene	EG11255. rsfS.
Phylogenomic databases
eggNOG	COG0799.
HOGENOM	HOG000016051.
KO	K09710.
OMA	WGDAKIT.
ProtClustDB	PRK11538.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11255-MONOMER. ECOL316407:JW5090-MONOMER.
Gene expression databases
Genevestigator	P0AAT6.
Family and domain databases
HAMAP	MF_01477. Iojap_RsfS.
InterPro	IPR025656. Oligomer_dom. IPR004394. Ribosome-assoc_Iojap-like. [Graphical view]
PANTHER	PTHR21043. PTHR21043. 1 hit.
Pfam	PF02410. Oligomerisation. 1 hit. [Graphical view]
TIGRFAMs	TIGR00090. iojap_ybeB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

IOJAP_ECOLI

Accession

Primary (citable) accession number: P0AAT6
Secondary accession number(s): P05848, P77107

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program