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P0AAT6 (IOJAP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal silencing factor RsfS
Gene names
Name:rsfS
Synonyms:rsfA, slm3, ybeB
Ordered Locus Names:b0637, JW5090
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length105 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a ribosomal silencing factor. Addition to isolated ribosomal subunits partially inhibits their association, preventing translation. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8, preventing association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation. Ref.7

Subunit structure

Interacts with ribosomal protein L14 (rplN). Ref.6 Ref.7

Subcellular location

Cytoplasm. Note: Comigrates with the 50S ribosomal subunit in sucrose gradients, specifically with ribosomal protein L14 (rplN). Ref.6

Disruption phenotype

Cell viability is dramatically reduced in stationary phase, cells have a 10 hour growth block upon transition from rich to poor medium. Protein translation in stationary phase is derepressed. Ref.7

Sequence similarities

Belongs to the Iojap/RsfS family.

Sequence caution

The sequence CAA28199.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processTranslation regulation
   Cellular componentCytoplasm
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmature ribosome assembly

Inferred from electronic annotation. Source: UniProtKB-HAMAP

negative regulation of ribosome biogenesis

Inferred from direct assay Ref.7. Source: UniProtKB

negative regulation of translation

Inferred from direct assay Ref.7. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionribosomal large subunit binding

Inferred from direct assay Ref.6. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 105105Ribosomal silencing factor RsfS HAMAP-Rule MF_01477
PRO_0000168674

Sequences

Sequence LengthMass (Da)Tools
P0AAT6 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 7C1315607BD0B610

FASTA10511,582
        10         20         30         40         50         60 
MQGKALQDFV IDKIDDLKGQ DIIALDVQGK SSITDCMIIC TGTSSRHVMS IADHVVQESR 

        70         80         90        100 
AAGLLPLGVE GENSADWIVV DLGDVIVHVM QEESRRLYEL EKLWS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12."
Asoh S., Matsuzawa H., Ishino F., Strominger J.L., Matsuhashi M., Ohta T.
Eur. J. Biochem. 160:231-238(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"The Escherichia coli GTPase CgtAE is involved in late steps of large ribosome assembly."
Jiang M., Datta K., Walker A., Strahler J., Bagamasbad P., Andrews P.C., Maddock J.R.
J. Bacteriol. 188:6757-6770(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, ASSOCIATION WITH THE 50S RIBOSOMAL SUBUNIT.
Strain: K12 / MG1655 / ATCC 47076.
[7]"RsfA (YbeB) proteins are conserved ribosomal silencing factors."
Hauser R., Pech M., Kijek J., Yamamoto H., Titz B., Naeve F., Tovchigrechko A., Yamamoto K., Szaflarski W., Takeuchi N., Stellberger T., Diefenbacher M.E., Nierhaus K.H., Uetz P.
PLoS Genet. 8:E1002815-E1002815(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RIBOSOMAL PROTEIN L14 (RPLN), DISRUPTION PHENOTYPE.
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04516 Genomic DNA. Translation: CAA28199.1. Different initiation.
U82598 Genomic DNA. Translation: AAB40837.1.
U00096 Genomic DNA. Translation: AAC73738.2.
AP009048 Genomic DNA. Translation: BAA35284.2.
RefSeqNP_415170.4. NC_000913.3.
YP_488928.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AAT6.
SMRP0AAT6. Positions 1-104.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48141N.
IntActP0AAT6. 10 interactions.
MINTMINT-1226369.
STRING511145.b0637.

Proteomic databases

PaxDbP0AAT6.
PRIDEP0AAT6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73738; AAC73738; b0637.
BAA35284; BAA35284; BAA35284.
GeneID12932664.
945237.
KEGGecj:Y75_p0627.
eco:b0637.
PATRIC32116457. VBIEscCol129921_0668.

Organism-specific databases

EchoBASEEB1235.
EcoGeneEG11255. rsfS.

Phylogenomic databases

eggNOGCOG0799.
HOGENOMHOG000016051.
KOK09710.
OMAYYNIERL.
OrthoDBEOG6J48TR.
PhylomeDBP0AAT6.
ProtClustDBPRK11538.

Enzyme and pathway databases

BioCycEcoCyc:EG11255-MONOMER.
ECOL316407:JW5090-MONOMER.

Gene expression databases

GenevestigatorP0AAT6.

Family and domain databases

HAMAPMF_01477. Iojap_RsfS.
InterProIPR004394. Iojap/RsfS.
IPR025656. Oligomer_dom.
[Graphical view]
PANTHERPTHR21043. PTHR21043. 1 hit.
PfamPF02410. Oligomerisation. 1 hit.
[Graphical view]
TIGRFAMsTIGR00090. iojap_ybeB. 1 hit.
ProtoNetSearch...

Other

PROP0AAT6.

Entry information

Entry nameIOJAP_ECOLI
AccessionPrimary (citable) accession number: P0AAT6
Secondary accession number(s): P05848, P77107
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene