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P0AAT6

- IOJAP_ECOLI

UniProt

P0AAT6 - IOJAP_ECOLI

Protein

Ribosomal silencing factor RsfS

Gene

rsfS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Functions as a ribosomal silencing factor. Addition to isolated ribosomal subunits partially inhibits their association, preventing translation. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8, preventing association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.1 Publication

    GO - Molecular functioni

    1. protein binding Source: EcoCyc
    2. ribosomal large subunit binding Source: EcoCyc

    GO - Biological processi

    1. mature ribosome assembly Source: UniProtKB-HAMAP
    2. negative regulation of ribosome biogenesis Source: UniProtKB
    3. negative regulation of translation Source: UniProtKB

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Translation regulation

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11255-MONOMER.
    ECOL316407:JW5090-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosomal silencing factor RsfS
    Gene namesi
    Name:rsfS
    Synonyms:rsfA, slm3, ybeB
    Ordered Locus Names:b0637, JW5090
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11255. rsfS.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: Comigrates with the 50S ribosomal subunit in sucrose gradients, specifically with ribosomal protein L14 (rplN).

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cell viability is dramatically reduced in stationary phase, cells have a 10 hour growth block upon transition from rich to poor medium. Protein translation in stationary phase is derepressed.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 105105Ribosomal silencing factor RsfSPRO_0000168674Add
    BLAST

    Proteomic databases

    PaxDbiP0AAT6.
    PRIDEiP0AAT6.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AAT6.

    Interactioni

    Subunit structurei

    Interacts with ribosomal protein L14 (rplN).1 Publication

    Protein-protein interaction databases

    DIPiDIP-48141N.
    IntActiP0AAT6. 10 interactions.
    MINTiMINT-1226369.
    STRINGi511145.b0637.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AAT6.
    SMRiP0AAT6. Positions 1-104.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Iojap/RsfS family.Curated

    Phylogenomic databases

    eggNOGiCOG0799.
    HOGENOMiHOG000016051.
    KOiK09710.
    OMAiCEIIPRI.
    OrthoDBiEOG6J48TR.
    PhylomeDBiP0AAT6.

    Family and domain databases

    HAMAPiMF_01477. Iojap_RsfS.
    InterProiIPR004394. Iojap/RsfS.
    IPR025656. Oligomer_dom.
    [Graphical view]
    PANTHERiPTHR21043. PTHR21043. 1 hit.
    PfamiPF02410. Oligomerisation. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00090. iojap_ybeB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AAT6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGKALQDFV IDKIDDLKGQ DIIALDVQGK SSITDCMIIC TGTSSRHVMS    50
    IADHVVQESR AAGLLPLGVE GENSADWIVV DLGDVIVHVM QEESRRLYEL 100
    EKLWS 105
    Length:105
    Mass (Da):11,582
    Last modified:October 11, 2005 - v1
    Checksum:i7C1315607BD0B610
    GO

    Sequence cautioni

    The sequence CAA28199.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04516 Genomic DNA. Translation: CAA28199.1. Different initiation.
    U82598 Genomic DNA. Translation: AAB40837.1.
    U00096 Genomic DNA. Translation: AAC73738.2.
    AP009048 Genomic DNA. Translation: BAA35284.2.
    RefSeqiNP_415170.4. NC_000913.3.
    YP_488928.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73738; AAC73738; b0637.
    BAA35284; BAA35284; BAA35284.
    GeneIDi12932664.
    945237.
    KEGGiecj:Y75_p0627.
    eco:b0637.
    PATRICi32116457. VBIEscCol129921_0668.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04516 Genomic DNA. Translation: CAA28199.1 . Different initiation.
    U82598 Genomic DNA. Translation: AAB40837.1 .
    U00096 Genomic DNA. Translation: AAC73738.2 .
    AP009048 Genomic DNA. Translation: BAA35284.2 .
    RefSeqi NP_415170.4. NC_000913.3.
    YP_488928.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AAT6.
    SMRi P0AAT6. Positions 1-104.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48141N.
    IntActi P0AAT6. 10 interactions.
    MINTi MINT-1226369.
    STRINGi 511145.b0637.

    Proteomic databases

    PaxDbi P0AAT6.
    PRIDEi P0AAT6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73738 ; AAC73738 ; b0637 .
    BAA35284 ; BAA35284 ; BAA35284 .
    GeneIDi 12932664.
    945237.
    KEGGi ecj:Y75_p0627.
    eco:b0637.
    PATRICi 32116457. VBIEscCol129921_0668.

    Organism-specific databases

    EchoBASEi EB1235.
    EcoGenei EG11255. rsfS.

    Phylogenomic databases

    eggNOGi COG0799.
    HOGENOMi HOG000016051.
    KOi K09710.
    OMAi CEIIPRI.
    OrthoDBi EOG6J48TR.
    PhylomeDBi P0AAT6.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11255-MONOMER.
    ECOL316407:JW5090-MONOMER.

    Miscellaneous databases

    PROi P0AAT6.

    Gene expression databases

    Genevestigatori P0AAT6.

    Family and domain databases

    HAMAPi MF_01477. Iojap_RsfS.
    InterProi IPR004394. Iojap/RsfS.
    IPR025656. Oligomer_dom.
    [Graphical view ]
    PANTHERi PTHR21043. PTHR21043. 1 hit.
    Pfami PF02410. Oligomerisation. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00090. iojap_ybeB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12."
      Asoh S., Matsuzawa H., Ishino F., Strominger J.L., Matsuhashi M., Ohta T.
      Eur. J. Biochem. 160:231-238(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "The Escherichia coli GTPase CgtAE is involved in late steps of large ribosome assembly."
      Jiang M., Datta K., Walker A., Strahler J., Bagamasbad P., Andrews P.C., Maddock J.R.
      J. Bacteriol. 188:6757-6770(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, ASSOCIATION WITH THE 50S RIBOSOMAL SUBUNIT.
      Strain: K12 / MG1655 / ATCC 47076.
    7. Cited for: FUNCTION, INTERACTION WITH RIBOSOMAL PROTEIN L14 (RPLN), DISRUPTION PHENOTYPE.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiIOJAP_ECOLI
    AccessioniPrimary (citable) accession number: P0AAT6
    Secondary accession number(s): P05848, P77107
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3