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Protein

Miniconductance mechanosensitive channel YbdG

Gene

ybdG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks.1 Publication

GO - Molecular functioni

  • mechanically-gated ion channel activity Source: EcoCyc

GO - Biological processi

  • cellular response to osmotic stress Source: EcoCyc
  • ion transmembrane transport Source: GOC
  • response to osmotic stress Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6323-MONOMER.
ECOL316407:JW0566-MONOMER.

Protein family/group databases

TCDBi1.A.23.4.5. the small conductance mechanosensitive ion channel (mscs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Miniconductance mechanosensitive channel YbdG
Gene namesi
Name:ybdG
Synonyms:mscM
Ordered Locus Names:b0577, JW0566
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12620. ybdG.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424PeriplasmicSequence analysisAdd
BLAST
Transmembranei25 – 4521HelicalSequence analysisAdd
BLAST
Topological domaini46 – 6722CytoplasmicSequence analysisAdd
BLAST
Transmembranei68 – 8821HelicalSequence analysisAdd
BLAST
Topological domaini89 – 10416PeriplasmicSequence analysisAdd
BLAST
Transmembranei105 – 12521HelicalSequence analysisAdd
BLAST
Topological domaini126 – 14823CytoplasmicSequence analysisAdd
BLAST
Transmembranei149 – 16921HelicalSequence analysisAdd
BLAST
Topological domaini170 – 1734PeriplasmicSequence analysis
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Topological domaini195 – 415221CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Miniconductance mechanosensitive channel YbdGPRO_0000168667Add
BLAST

Proteomic databases

PaxDbiP0AAT4.

Expressioni

Inductioni

Induced by growth at high osmolarity.1 Publication

Interactioni

Subunit structurei

Homoheptamer.1 Publication

Protein-protein interaction databases

BioGridi4262912. 12 interactions.
DIPiDIP-51225N.
IntActiP0AAT4. 4 interactions.
STRINGi511145.b0577.

Structurei

3D structure databases

ProteinModelPortaliP0AAT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CUY. Bacteria.
COG0668. LUCA.
HOGENOMiHOG000199955.
InParanoidiP0AAT4.
KOiK16053.
OMAiIHFLDEQ.
OrthoDBiEOG6MPWW3.
PhylomeDBiP0AAT4.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR030192. YbdG.
[Graphical view]
PANTHERiPTHR30414:SF0. PTHR30414:SF0. 1 hit.
PfamiPF00924. MS_channel. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AAT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDLISQVED LAGIEIDHTT SMVMIFGIIF LTAVVVHIIL HWVVLRTFEK
60 70 80 90 100
RAIASSRLWL QIITQNKLFH RLAFTLQGII VNIQAVFWLQ KGTEAADILT
110 120 130 140 150
TCAQLWIMMY ALLSVFSLLD VILNLAQKFP AASQLPLKGI FQGIKLIGAI
160 170 180 190 200
LVGILMISLL IGQSPAILIS GLGAMAAVLM LVFKDPILGL VAGIQLSAND
210 220 230 240 250
MLKLGDWLEM PKYGADGAVI DIGLTTVKVR NWDNTITTIP TWSLVSDSFK
260 270 280 290 300
NWSGMSASGG RRIKRSISID VTSIRFLDED EMQRLNKAHL LKPYLTSRHQ
310 320 330 340 350
EINEWNRQQG STESVLNLRR MTNIGTFRAY LNEYLRNHPR IRKDMTLMVR
360 370 380 390 400
QLAPGDNGLP LEIYAFTNTV VWLEYESIQA DIFDHIFAIV EEFGLRLHQS
410
PTGNDIRSLA GAFKQ
Length:415
Mass (Da):46,603
Last modified:October 11, 2005 - v1
Checksum:iC9A8A8A41CDC8F28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40775.1.
U00096 Genomic DNA. Translation: AAC73678.1.
AP009048 Genomic DNA. Translation: BAA35217.1.
U07860 Genomic DNA. Translation: AAC43265.1.
D25414 Genomic DNA. No translation available.
PIRiG64790.
RefSeqiNP_415109.1. NC_000913.3.
WP_001153148.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73678; AAC73678; b0577.
BAA35217; BAA35217; BAA35217.
GeneIDi946243.
KEGGiecj:JW0566.
eco:b0577.
PATRICi32116322. VBIEscCol129921_0601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82598 Genomic DNA. Translation: AAB40775.1.
U00096 Genomic DNA. Translation: AAC73678.1.
AP009048 Genomic DNA. Translation: BAA35217.1.
U07860 Genomic DNA. Translation: AAC43265.1.
D25414 Genomic DNA. No translation available.
PIRiG64790.
RefSeqiNP_415109.1. NC_000913.3.
WP_001153148.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262912. 12 interactions.
DIPiDIP-51225N.
IntActiP0AAT4. 4 interactions.
STRINGi511145.b0577.

Protein family/group databases

TCDBi1.A.23.4.5. the small conductance mechanosensitive ion channel (mscs) family.

Proteomic databases

PaxDbiP0AAT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73678; AAC73678; b0577.
BAA35217; BAA35217; BAA35217.
GeneIDi946243.
KEGGiecj:JW0566.
eco:b0577.
PATRICi32116322. VBIEscCol129921_0601.

Organism-specific databases

EchoBASEiEB2504.
EcoGeneiEG12620. ybdG.

Phylogenomic databases

eggNOGiENOG4105CUY. Bacteria.
COG0668. LUCA.
HOGENOMiHOG000199955.
InParanoidiP0AAT4.
KOiK16053.
OMAiIHFLDEQ.
OrthoDBiEOG6MPWW3.
PhylomeDBiP0AAT4.

Enzyme and pathway databases

BioCyciEcoCyc:G6323-MONOMER.
ECOL316407:JW0566-MONOMER.

Miscellaneous databases

PROiP0AAT4.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR011066. MscC_channel_C.
IPR006685. MscS_channel.
IPR030192. YbdG.
[Graphical view]
PANTHERiPTHR30414:SF0. PTHR30414:SF0. 1 hit.
PfamiPF00924. MS_channel. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
SSF82689. SSF82689. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Physical characterisation of the Escherichia coli B gene encoding nitroreductase and its over-expression in Escherichia coli K12."
    Michael N.P., Brehm J.K., Anlezark G.M., Minton N.P.
    FEMS Microbiol. Lett. 124:195-202(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
    Strain: B.
  6. "Gene cloning, purification, and characterization of NfsB, a minor oxygen-insensitive nitroreductase from Escherichia coli, similar in biochemical properties to FRaseI, the major flavin reductase in Vibrio fischeri."
    Zenno S., Koike H., Tanokura M., Saigo K.
    Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
    Strain: K12 / C600 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222.
  7. "YbdG in Escherichia coli is a threshold-setting mechanosensitive channel with MscM activity."
    Schumann U., Edwards M.D., Rasmussen T., Bartlett W., van West P., Booth I.R.
    Proc. Natl. Acad. Sci. U.S.A. 107:12664-12669(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: K12.
  8. "Characterization of three novel mechanosensitive channel activities in Escherichia coli."
    Edwards M.D., Black S., Rasmussen T., Rasmussen A., Stokes N.R., Stephen T.L., Miller S., Booth I.R.
    Channels 6:272-281(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SHOWS THAT THIS IS NOT MSCM.

Entry informationi

Entry nameiYBDG_ECOLI
AccessioniPrimary (citable) accession number: P0AAT4
Secondary accession number(s): P39455, P77602
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: January 20, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be the miniconductance mechanosensitive channel MscM.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.