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Protein

Probable diguanylate cyclase AdrA

Gene

adrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

A probable diguanylate cyclase. The last member of a cascade of expressed proteins, its expression requires YdaM. AdrA production induces biosynthesis of cellulose in some E.coli isolates, but not in K12 strains. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.1 Publication

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi248MagnesiumBy similarity1
Sitei253Transition state stabilizerSequence analysis1
Binding sitei256SubstrateBy similarity1
Binding sitei265SubstrateBy similarity1
Active sitei291Proton acceptorSequence analysis1
Metal bindingi291MagnesiumBy similarity1

GO - Molecular functioni

  • diguanylate cyclase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11257-MONOMER.
ECOL316407:JW0376-MONOMER.
MetaCyc:EG11257-MONOMER.
UniPathwayiUPA00599.

Protein family/group databases

TCDBi9.B.34.1.2. the kinase/phosphatase/cyclic-gmp synthase/cyclic di-gmp hydrolase (kpsh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase AdrA (EC:2.7.7.65)
Short name:
DGC
Gene namesi
Name:adrA
Synonyms:yaiC
Ordered Locus Names:b0385, JW0376
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11257. adrA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei46 – 66HelicalSequence analysisAdd BLAST21
Transmembranei68 – 88HelicalSequence analysisAdd BLAST21
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Transmembranei143 – 163HelicalSequence analysisAdd BLAST21
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi291 – 292DE → AA: No c-di-GMP produced in an overexpressing strain. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001685881 – 371Probable diguanylate cyclase AdrAAdd BLAST371

Proteomic databases

PaxDbiP0AAP1.
PRIDEiP0AAP1.

Expressioni

Inductioni

Expressed at 28 degrees Celsius in late stationary phase, more highly expressed on plates than in liquid medium. Expression is RpoS-, CsgD- and YdaM-dependent.2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4262033. 8 interactors.
STRINGi511145.b0385.

Structurei

3D structure databases

ProteinModelPortaliP0AAP1.
SMRiP0AAP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini240 – 371GGDEFPROSITE-ProRule annotationAdd BLAST132

Sequence similaritiesi

Contains 1 GGDEF domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000117511.
InParanoidiP0AAP1.
KOiK18968.
OMAiHGAWKDL.
PhylomeDBiP0AAP1.

Family and domain databases

CDDicd01949. GGDEF. 1 hit.
InterProiIPR000160. GGDEF_dom.
IPR007894. MASE2.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00990. GGDEF. 1 hit.
PF05230. MASE2. 1 hit.
[Graphical view]
SMARTiSM00267. GGDEF. 1 hit.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPKIMNDEN FFKKAAAHGE EPPLTPQNEH QRSGLRFARR VRLPRAVGLA
60 70 80 90 100
GMFLPIASTL VSHPPPGWWW LVLVGWAFVW PHLAWQIASR AVDPLSREIY
110 120 130 140 150
NLKTDAVLAG MWVGVMGVNV LPSTAMLMIM CLNLMGAGGP RLFVAGLVLM
160 170 180 190 200
VVSCLVTLEL TGITVSFNSA PLEWWLSLPI IVIYPLLFGW VSYQTATKLA
210 220 230 240 250
EHKRRLQVMS TRDGMTGVYN RRHWETMLRN EFDNCRRHNR DATLLIIDID
260 270 280 290 300
HFKSINDTWG HDVGDEAIVA LTRQLQITLR GSDVIGRFGG DEFAVIMSGT
310 320 330 340 350
PAESAITAML RVHEGLNTLR LPNTPQVTLR ISVGVAPLNP QMSHYREWLK
360 370
SADLALYKAK KAGRNRTEVA A
Length:371
Mass (Da):41,537
Last modified:October 11, 2005 - v1
Checksum:i1F5111C43481F32B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18109.1.
U00096 Genomic DNA. Translation: AAC73488.1.
AP009048 Genomic DNA. Translation: BAE76166.1.
M13345 Genomic DNA. Translation: AAA83895.1.
PIRiA64767.
RefSeqiNP_414919.1. NC_000913.3.
WP_000484048.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73488; AAC73488; b0385.
BAE76166; BAE76166; BAE76166.
GeneIDi945037.
KEGGiecj:JW0376.
eco:b0385.
PATRICi32115911. VBIEscCol129921_0397.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18109.1.
U00096 Genomic DNA. Translation: AAC73488.1.
AP009048 Genomic DNA. Translation: BAE76166.1.
M13345 Genomic DNA. Translation: AAA83895.1.
PIRiA64767.
RefSeqiNP_414919.1. NC_000913.3.
WP_000484048.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAP1.
SMRiP0AAP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262033. 8 interactors.
STRINGi511145.b0385.

Protein family/group databases

TCDBi9.B.34.1.2. the kinase/phosphatase/cyclic-gmp synthase/cyclic di-gmp hydrolase (kpsh) family.

Proteomic databases

PaxDbiP0AAP1.
PRIDEiP0AAP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73488; AAC73488; b0385.
BAE76166; BAE76166; BAE76166.
GeneIDi945037.
KEGGiecj:JW0376.
eco:b0385.
PATRICi32115911. VBIEscCol129921_0397.

Organism-specific databases

EchoBASEiEB1237.
EcoGeneiEG11257. adrA.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000117511.
InParanoidiP0AAP1.
KOiK18968.
OMAiHGAWKDL.
PhylomeDBiP0AAP1.

Enzyme and pathway databases

UniPathwayiUPA00599.
BioCyciEcoCyc:EG11257-MONOMER.
ECOL316407:JW0376-MONOMER.
MetaCyc:EG11257-MONOMER.

Miscellaneous databases

PROiP0AAP1.

Family and domain databases

CDDicd01949. GGDEF. 1 hit.
InterProiIPR000160. GGDEF_dom.
IPR007894. MASE2.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00990. GGDEF. 1 hit.
PF05230. MASE2. 1 hit.
[Graphical view]
SMARTiSM00267. GGDEF. 1 hit.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADRA_ECOLI
AccessioniPrimary (citable) accession number: P0AAP1
Secondary accession number(s): P21830, P77719, Q2MC40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.