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Protein

Anti-adapter protein IraP

Gene

iraP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits RpoS proteolysis by regulating RssB activity, thereby increasing the stability of the sigma stress factor RpoS especially during phosphate starvation, but also in stationary phase and during nitrogen starvation. Its effect on RpoS stability is due to its interaction with RssB, which probably blocks the interaction of RssB with RpoS, and the consequent delivery of the RssB-RpoS complex to the ClpXP protein degradation pathway.2 Publications

GO - Molecular functioni

  • anti-sigma factor antagonist activity Source: EcoCyc

GO - Biological processi

  • cellular response to phosphate starvation Source: EcoCyc
  • negative regulation of protein catabolic process Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: GOC
Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:EG11256-MONOMER.
ECOL316407:JW0373-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Anti-adapter protein IraP
Gene namesi
Name:iraP
Synonyms:yaiB
Ordered Locus Names:b0382, JW0373
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11256. iraP.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi9 – 91L → S: Loss of ability to interact with RssB. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8686Anti-adapter protein IraPPRO_0000168585Add
BLAST

Proteomic databases

PaxDbiP0AAN9.
PRIDEiP0AAN9.

Expressioni

Inductioni

By phosphate and nitrogen starvation, and in stationary phase, via the alarmone ppGpp.2 Publications

Interactioni

Subunit structurei

Interacts with RssB.1 Publication

Protein-protein interaction databases

BioGridi4261526. 9 interactions.
DIPiDIP-48131N.
IntActiP0AAN9. 8 interactions.
STRINGi511145.b0382.

Structurei

3D structure databases

ProteinModelPortaliP0AAN9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 3636Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the IraP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105RZQ. Bacteria.
ENOG4111YMR. LUCA.
HOGENOMiHOG000059299.
OMAiRNMAQSE.
OrthoDBiEOG63VC48.

Family and domain databases

HAMAPiMF_01198. Anti_adapt_IraP.
InterProiIPR019732. SigmaS_Anti-adapt_IraP.
[Graphical view]
PfamiPF10796. Anti-adapt_IraP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNLIAELLF KLAQKEEESK ELCAQVEALE IIVTAMLRNM AQNDQQRLID
60 70 80
QVEGALYEVK PDASIPDDDT ELLRDYVKKL LKHPRQ
Length:86
Mass (Da):9,937
Last modified:October 11, 2005 - v1
Checksum:i1C2EE018BFB16BEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18106.1.
U00096 Genomic DNA. Translation: AAC73485.1.
AP009048 Genomic DNA. Translation: BAE76163.1.
M13345 Genomic DNA. No translation available.
J01659 Genomic DNA. Translation: AAR23118.1.
PIRiF64766.
RefSeqiNP_414916.1. NC_000913.3.
WP_000792970.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73485; AAC73485; b0382.
BAE76163; BAE76163; BAE76163.
GeneIDi947709.
KEGGiecj:JW0373.
eco:b0382.
PATRICi32115905. VBIEscCol129921_0394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA. Translation: AAB18106.1.
U00096 Genomic DNA. Translation: AAC73485.1.
AP009048 Genomic DNA. Translation: BAE76163.1.
M13345 Genomic DNA. No translation available.
J01659 Genomic DNA. Translation: AAR23118.1.
PIRiF64766.
RefSeqiNP_414916.1. NC_000913.3.
WP_000792970.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAN9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261526. 9 interactions.
DIPiDIP-48131N.
IntActiP0AAN9. 8 interactions.
STRINGi511145.b0382.

Proteomic databases

PaxDbiP0AAN9.
PRIDEiP0AAN9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73485; AAC73485; b0382.
BAE76163; BAE76163; BAE76163.
GeneIDi947709.
KEGGiecj:JW0373.
eco:b0382.
PATRICi32115905. VBIEscCol129921_0394.

Organism-specific databases

EchoBASEiEB1236.
EcoGeneiEG11256. iraP.

Phylogenomic databases

eggNOGiENOG4105RZQ. Bacteria.
ENOG4111YMR. LUCA.
HOGENOMiHOG000059299.
OMAiRNMAQSE.
OrthoDBiEOG63VC48.

Enzyme and pathway databases

BioCyciEcoCyc:EG11256-MONOMER.
ECOL316407:JW0373-MONOMER.

Miscellaneous databases

PROiP0AAN9.

Family and domain databases

HAMAPiMF_01198. Anti_adapt_IraP.
InterProiIPR019732. SigmaS_Anti-adapt_IraP.
[Graphical view]
PfamiPF10796. Anti-adapt_IraP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli."
    Chang C.N., Kuang W.-J., Chen E.Y.
    Gene 44:121-125(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-86.
    Strain: 294.
  5. "The nucleotide sequence of the promoter and the amino-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli."
    Kikuchi Y., Yoda K., Yamasaki M., Tamura G.
    Nucleic Acids Res. 9:5671-5678(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-86.
    Strain: K12.
  6. "Modulating RssB activity: IraP, a novel regulator of sigma(S) stability in Escherichia coli."
    Bougdour A., Wickner S., Gottesman S.
    Genes Dev. 20:884-897(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS INHIBITOR OF RSSB ACTIVITY, INTERACTION WITH RSSB, INDUCTION, GENE NAME, MUTAGENESIS OF LEU-9.
    Strain: K12 / MG1655 / ATCC 47076.
  7. "ppGpp regulation of RpoS degradation via anti-adaptor protein IraP."
    Bougdour A., Gottesman S.
    Proc. Natl. Acad. Sci. U.S.A. 104:12896-12901(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY PPGPP.
    Strain: K12 / MG1655 / ATCC 47076.
  8. "Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors."
    Bougdour A., Cunning C., Baptiste P.J., Elliott T., Gottesman S.
    Mol. Microbiol. 68:298-313(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiIRAP_ECOLI
AccessioniPrimary (citable) accession number: P0AAN9
Secondary accession number(s): P21831, P77383, Q2MC43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: January 20, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.