Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydrogenase isoenzymes nickel incorporation protein HypB

Gene

hypB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases.1 Publication

GO - Molecular functioni

  • GTPase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW
  • nickel cation binding Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • cofactor biosynthetic process Source: EcoCyc
  • protein complex assembly Source: InterPro
  • protein maturation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Metal-binding, Nickel, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10484-MONOMER.
ECOL316407:JW2697-MONOMER.
MetaCyc:EG10484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase isoenzymes nickel incorporation protein HypB
Gene namesi
Name:hypB
Ordered Locus Names:b2727, JW2697
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10484. hypB.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi117 – 1171K → N: Important decrease in GTPase activity. 1 Publication
Mutagenesisi241 – 2411D → N: 85-fold decrease in Kcat/KM value of GTPase activity and loss of the specificity for GTP. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290Hydrogenase isoenzymes nickel incorporation protein HypBPRO_0000201440Add
BLAST

Proteomic databases

PaxDbiP0AAN3.
PRIDEiP0AAN3.

2D gel databases

SWISS-2DPAGEP0AAN3.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
slyDP0A9K94EBI-558261,EBI-369251

Protein-protein interaction databases

BioGridi4261427. 5 interactions.
DIPiDIP-36428N.
IntActiP0AAN3. 9 interactions.
MINTiMINT-1293453.
STRINGi511145.b2727.

Structurei

3D structure databases

ProteinModelPortaliP0AAN3.
SMRiP0AAN3. Positions 87-284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107RSS. Bacteria.
COG0378. LUCA.
HOGENOMiHOG000236980.
InParanoidiP0AAN3.
KOiK04652.
OMAiGTHAPGQ.
PhylomeDBiP0AAN3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003495. CobW/HypB/UreG_dom.
IPR004392. Hyd_acc_HypB.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02492. cobW. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00073. hypB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AAN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCTTCGCGEG NLYIEGDEHN PHSAFRSAPF APAARPKMKI TGIKAPEFTP
60 70 80 90 100
SQTEEGDLHY GHGEAGTHAP GMSQRRMLEV EIDVLDKNNR LAERNRARFA
110 120 130 140 150
ARKQLVLNLV SSPGSGKTTL LTETLMRLKD SVPCAVIEGD QQTVNDAARI
160 170 180 190 200
RATGTPAIQV NTGKGCHLDA QMIADAAPRL PLDDNGILFI ENVGNLVCPA
210 220 230 240 250
SFDLGEKHKV AVLSVTEGED KPLKYPHMFA AASLMLLNKV DLLPYLNFDV
260 270 280 290
EKCIACAREV NPEIEIILIS ATSGEGMDQW LNWLETQRCA
Length:290
Mass (Da):31,565
Last modified:October 11, 2005 - v1
Checksum:i5B3AEB59AA6915C3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361P → R in CAA38413 (PubMed:1849603).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA. Translation: CAA38413.1.
U29579 Genomic DNA. Translation: AAA69237.1.
U00096 Genomic DNA. Translation: AAC75769.1.
AP009048 Genomic DNA. Translation: BAE76804.1.
PIRiC65053.
RefSeqiNP_417207.1. NC_000913.3.
WP_000337665.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75769; AAC75769; b2727.
BAE76804; BAE76804; BAE76804.
GeneIDi947194.
KEGGiecj:JW2697.
eco:b2727.
PATRICi32120854. VBIEscCol129921_2819.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA. Translation: CAA38413.1.
U29579 Genomic DNA. Translation: AAA69237.1.
U00096 Genomic DNA. Translation: AAC75769.1.
AP009048 Genomic DNA. Translation: BAE76804.1.
PIRiC65053.
RefSeqiNP_417207.1. NC_000913.3.
WP_000337665.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAN3.
SMRiP0AAN3. Positions 87-284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261427. 5 interactions.
DIPiDIP-36428N.
IntActiP0AAN3. 9 interactions.
MINTiMINT-1293453.
STRINGi511145.b2727.

2D gel databases

SWISS-2DPAGEP0AAN3.

Proteomic databases

PaxDbiP0AAN3.
PRIDEiP0AAN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75769; AAC75769; b2727.
BAE76804; BAE76804; BAE76804.
GeneIDi947194.
KEGGiecj:JW2697.
eco:b2727.
PATRICi32120854. VBIEscCol129921_2819.

Organism-specific databases

EchoBASEiEB0479.
EcoGeneiEG10484. hypB.

Phylogenomic databases

eggNOGiENOG4107RSS. Bacteria.
COG0378. LUCA.
HOGENOMiHOG000236980.
InParanoidiP0AAN3.
KOiK04652.
OMAiGTHAPGQ.
PhylomeDBiP0AAN3.

Enzyme and pathway databases

BioCyciEcoCyc:EG10484-MONOMER.
ECOL316407:JW2697-MONOMER.
MetaCyc:EG10484-MONOMER.

Miscellaneous databases

PROiP0AAN3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003495. CobW/HypB/UreG_dom.
IPR004392. Hyd_acc_HypB.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02492. cobW. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00073. hypB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHYPB_ECOLI
AccessioniPrimary (citable) accession number: P0AAN3
Secondary accession number(s): P24190, Q2MAA2, Q46884
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.