P0AAN1 (HYBE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydrogenase-2 operon protein hybE

Gene names

Name:	hybE
Ordered Locus Names:	b2992, JW2960

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the hupJ family.

Ontologies

Keywords
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein maturation Inferred from mutant phenotype. Source: EcoCyc
Molecular function	preprotein binding Inferred from physical interaction. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
hybO	P69741	2	EBI-552588,EBI-552619

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 162

162

Hydrogenase-2 operon protein hybE

PRO_0000201426

Experimental info

Sequence conflict

7

1

G → A in AAA21593. Ref.1

Secondary structure

1

.

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162

Helix Strand Turn

Details...

Helix

13 – 23

11

Helix

29 – 31

3

Beta strand

41 – 47

7

Beta strand

55 – 57

3

Beta strand

62 – 67

6

Beta strand

106 – 111

6

Helix

125 – 135

11

Sequences

	Sequence		Length	Mass (Da)
	P0AAN1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: CFDAEA3D47BE9516	FASTA	162	17,964
	10 20 30 40 50 60 MTEEIAGFQT SPKAQVQAAF EEIARRSMHD LSFLHPSMPV YVSDFTLFEG QWTGCVITPW 70 80 90 100 110 120 MLSAVIFPGP DQLWPLRKVS EKIGLQLPYG TMTFTVGELD GVSQYLSCSL MSPLSHSMSI 130 140 150 160 EEGQRLTDDC ARMILSLPVT NPDVPHAGRR ALLFGRRSGE NA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2." Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T., Peck H.D. Jr., Przybyla A.E. J. Bacteriol. 176:4416-4423(1994) [PubMed: 8021226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / TG1.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U09177 Genomic DNA. Translation: AAA21593.1.
U28377 Genomic DNA. Translation: AAA69159.1.
U00096 Genomic DNA. Translation: AAC76028.1.
AP009048 Genomic DNA. Translation: BAE77053.1.

PIR

F65085.

RefSeq

NP_417466.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KC5	NMR	-	A	1-162	[»]

ProteinModelPortal

P0AAN1.

SMR

P0AAN1. Positions 1-162.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35795N.

IntAct

P0AAN1. 7 interactions.

MINT

MINT-1305432.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002290; EBESCP00000002290; EBESCG00000001870.
EBESCT00000016696; EBESCP00000015987; EBESCG00000015755.

GeneID

947483.

GenomeReviews

Gene locus JW2960 in contig AP009048_GR.
Gene locus b2992 in contig U00096_GR.

KEGG

ecj:JW2960.
eco:b2992.

PATRIC

32121400. VBIEscCol129921_3087.

Organism-specific databases

EchoBASE

EB1751.

EcoGene

EG11803. hybE.

Phylogenomic databases

eggNOG

NOG11367.

GeneTree

EBGT00050000011454.

HOGENOM

HBG296627.

OMA

LTPWMLS.

ProtClustDB

PRK10465.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11803-MONOMER.

Gene expression databases

Genevestigator

P0AAN1.

Family and domain databases

InterPro

IPR023994. NiFe-hyd_HybE.
[Graphical view]

Pfam

PF11939. DUF3457. 1 hit.
[Graphical view]

ProDom

PD008308. PD008308. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR03993. Hydrog_HybE. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HYBE_ECOLI

Accession

Primary (citable) accession number: P0AAN1
Secondary accession number(s): P37183, Q2M9K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program