ID   HYBG_ECOLI              Reviewed;          82 AA.
AC   P0AAM7; P37185; Q2M9K5;
DT   11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   27-MAR-2024, entry version 116.
DE   RecName: Full=Hydrogenase maturation factor HybG {ECO:0000305};
DE   AltName: Full=Hydrogenase-2 operon protein HybG {ECO:0000305};
GN   Name=hybG {ECO:0000303|PubMed:8021226};
GN   OrderedLocusNames=b2990, JW2958;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / TG1;
RX   PubMed=8021226; DOI=10.1128/jb.176.14.4416-4423.1994;
RA   Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T.,
RA   Peck H.D. Jr., Przybyla A.E.;
RT   "Cloning, sequencing, and mutational analysis of the hyb operon encoding
RT   Escherichia coli hydrogenase 2.";
RL   J. Bacteriol. 176:4416-4423(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION, INTERACTION WITH HYBC, AND DISRUPTION PHENOTYPE.
RX   PubMed=11292801; DOI=10.1128/jb.183.9.2817-2822.2001;
RA   Blokesch M., Magalon A., Boeck A.;
RT   "Interplay between the specific chaperone-like proteins HybG and HypC in
RT   maturation of hydrogenases 1, 2, and 3 from Escherichia coli.";
RL   J. Bacteriol. 183:2817-2822(2001).
RN   [5]
RP   PROBABLE FUNCTION, AND INTERACTION WITH HYPD; HYPE; HYBC AND HYAB.
RX   PubMed=16412426; DOI=10.1016/j.febslet.2005.12.063;
RA   Butland G., Zhang J.W., Yang W., Sheung A., Wong P., Greenblatt J.F.,
RA   Emili A., Zamble D.B.;
RT   "Interactions of the Escherichia coli hydrogenase biosynthetic proteins:
RT   HybG complex formation.";
RL   FEBS Lett. 580:677-681(2006).
RN   [6]
RP   FUNCTION.
RX   PubMed=23851071; DOI=10.1016/j.febslet.2013.06.055;
RA   Soboh B., Stripp S.T., Bielak C., Lindenstrauss U., Braussemann M.,
RA   Javaid M., Hallensleben M., Granich C., Herzberg M., Heberle J.,
RA   Sawers R.G.;
RT   "The [NiFe]-hydrogenase accessory chaperones HypC and HybG of Escherichia
RT   coli are iron- and carbon dioxide-binding proteins.";
RL   FEBS Lett. 587:2512-2516(2013).
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Involved
CC       in the biosynthesis of the Fe(CN)(2)CO cofactor (PubMed:16412426). HybG
CC       delivers iron-bound CO(2) to HypD where reduction to CO probably occurs
CC       (PubMed:23851071). In complex with HypD, accepts the cyanide ligand
CC       generated by HypF and HypE, and also coordinates the carbon monoxide
CC       ligand (By similarity). Involved in the maturation of the hydrogenase
CC       2. Also participates in the maturation of hydrogenase 1
CC       (PubMed:11292801). {ECO:0000250|UniProtKB:P0AAM3,
CC       ECO:0000269|PubMed:11292801, ECO:0000269|PubMed:23851071,
CC       ECO:0000305|PubMed:16412426}.
CC   -!- PATHWAY: Protein modification; [NiFe] hydrogenase maturation.
CC       {ECO:0000305}.
CC   -!- SUBUNIT: Forms a complex with HypD and HypE (PubMed:16412426).
CC       Interacts with the precursor form of HybC (pre-HybC), the large subunit
CC       of hydrogenase 2, and with HyaB, the large subunit of hydrogenase 1
CC       (PubMed:11292801, PubMed:16412426). {ECO:0000269|PubMed:11292801,
CC       ECO:0000269|PubMed:16412426}.
CC   -!- INTERACTION:
CC       P0AAM7; P0ACE0: hybC; NbExp=2; IntAct=EBI-562426, EBI-549849;
CC       P0AAM7; P24192: hypD; NbExp=7; IntAct=EBI-562426, EBI-552711;
CC       P0AAM7; P43674: ycaL; NbExp=2; IntAct=EBI-562426, EBI-9128400;
CC   -!- DISRUPTION PHENOTYPE: Deletion of the gene abolishes the generation of
CC       active hydrogenase 2, but only reduces hydrogenase 1 activity. It does
CC       not affect the maturation of hydrogenase 3. Deletion of both hybG and
CC       hypC completely abolishes hydrogenase activity.
CC       {ECO:0000269|PubMed:11292801}.
CC   -!- SIMILARITY: Belongs to the HupF/HypC family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U09177; AAA21595.1; -; Genomic_DNA.
DR   EMBL; U28377; AAA69157.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76026.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77051.1; -; Genomic_DNA.
DR   PIR; G55516; G55516.
DR   RefSeq; NP_417464.1; NC_000913.3.
DR   RefSeq; WP_000334896.1; NZ_STEB01000001.1.
DR   AlphaFoldDB; P0AAM7; -.
DR   SMR; P0AAM7; -.
DR   BioGRID; 4262372; 7.
DR   BioGRID; 852328; 4.
DR   ComplexPortal; CPX-5283; Hybg-HypDE Ni-hydrogenase maturation complex.
DR   DIP; DIP-36429N; -.
DR   IntAct; P0AAM7; 11.
DR   STRING; 511145.b2990; -.
DR   TCDB; 3.D.7.2.5; the h2:heterodisulfide oxidoreductase (hho) family.
DR   PaxDb; 511145-b2990; -.
DR   EnsemblBacteria; AAC76026; AAC76026; b2990.
DR   GeneID; 83579462; -.
DR   GeneID; 948020; -.
DR   KEGG; ecj:JW2958; -.
DR   KEGG; eco:b2990; -.
DR   PATRIC; fig|1411691.4.peg.3739; -.
DR   EchoBASE; EB1753; -.
DR   eggNOG; COG0298; Bacteria.
DR   HOGENOM; CLU_159381_1_1_6; -.
DR   InParanoid; P0AAM7; -.
DR   OMA; EEFGDPW; -.
DR   OrthoDB; 9806017at2; -.
DR   PhylomeDB; P0AAM7; -.
DR   BioCyc; EcoCyc:EG11805-MONOMER; -.
DR   UniPathway; UPA00335; -.
DR   PRO; PR:P0AAM7; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:1902670; F:carbon dioxide binding; IDA:EcoCyc.
DR   GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR   GO; GO:0005506; F:iron ion binding; IDA:EcoCyc.
DR   GO; GO:0051604; P:protein maturation; IDA:EcoCyc.
DR   GO; GO:0065003; P:protein-containing complex assembly; IDA:ComplexPortal.
DR   Gene3D; 2.30.30.140; -; 1.
DR   InterPro; IPR019812; Hydgase_assmbl_chp_CS.
DR   InterPro; IPR001109; Hydrogenase_HupF/HypC.
DR   NCBIfam; TIGR00074; hypC_hupF; 1.
DR   PANTHER; PTHR35177; HYDROGENASE MATURATION FACTOR HYBG; 1.
DR   PANTHER; PTHR35177:SF2; HYDROGENASE MATURATION FACTOR HYBG; 1.
DR   Pfam; PF01455; HupF_HypC; 1.
DR   PRINTS; PR00445; HUPFHYPC.
DR   SUPFAM; SSF159127; HupF/HypC-like; 1.
DR   PROSITE; PS01097; HUPF_HYPC; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..82
FT                   /note="Hydrogenase maturation factor HybG"
FT                   /id="PRO_0000201403"
FT   SITE            2
FT                   /note="Important for interaction with HypD and the
FT                   precursor form of hydrogenase"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAM3"
SQ   SEQUENCE   82 AA;  8808 MW;  8E9736BFC4EFD626 CRC64;
     MCIGVPGQVL AVGEDIHQLA QVEVCGIKRD VNIALICEGN PADLLGQWVL VHVGFAMSII
     DEDEAKATLD ALRQMDYDIT SA
//