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Protein

Hydrogenase maturation factor HypC

Gene

hypC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of [NiFe] hydrogenases. Involved in the biosynthesis of the Fe(CN)2CO cofactor (PubMed:15504408, PubMed:23022438). HypC delivers iron-bound CO2 to HypD where reduction to CO probably occurs (PubMed:23851071). In complex with HypD, accepts the cyanide ligand generated by HypF and HypE, and also coordinates the carbon monoxide ligand (PubMed:15504408, PubMed:23022438). Involved in the maturation of the hydrogenase 3 (PubMed:10783387, PubMed:11292801). Also participates in the maturation of hydrogenase 1 (PubMed:11292801).5 Publications

Pathwayi: [NiFe] hydrogenase maturation

This protein is involved in the pathway [NiFe] hydrogenase maturation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway [NiFe] hydrogenase maturation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei2Important for interaction with HypD and the precursor form of hydrogenase2 Publications1

GO - Molecular functioni

  • carbon dioxide binding Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • iron ion binding Source: EcoCyc

GO - Biological processi

  • protein maturation Source: EcoCyc

Enzyme and pathway databases

BioCyciEcoCyc:EG10485-MONOMER
UniPathwayiUPA00335

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase maturation factor HypCCurated
Alternative name(s):
Chaperone-type protein HypC1 Publication
Hydrogenase accessory chaperone HypCCurated
Gene namesi
Name:hypC1 Publication
Ordered Locus Names:b2728, JW2698
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10485 hypC

Pathology & Biotechi

Disruption phenotypei

Deletion of both hybG and hypC completely abolishes hydrogenase activity.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2C → A: Lack of hydrogenase 3 activity. Cannot interact with pre-HycE or HypD. Does not bind iron. 3 Publications1
Mutagenesisi2C → R or S: Lack of hydrogenase 3 activity. Cannot interact with pre-HycE or HypD. 2 Publications1
Mutagenesisi6P → A or T: No change in hydrogenase 3 activity. Does not affect interaction with HypD. 2 Publications1
Mutagenesisi51H → R: Does not bind iron. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002013982 – 90Hydrogenase maturation factor HypCAdd BLAST89

Proteomic databases

PaxDbiP0AAM3
PRIDEiP0AAM3

Interactioni

Subunit structurei

Homodimer (PubMed:9485446). Interacts with HypD (PubMed:12441107, PubMed:15504408, PubMed:23022438). Also forms a complex with HypD and HypE (PubMed:15504408). Interacts with the precursor form of HycE (pre-HycE), the large subunit of hydrogenase 3 (PubMed:9485446, PubMed:10783387, PubMed:12441107).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
lysSP0A8N33EBI-552654,EBI-552719

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261426, 9 interactors
DIPiDIP-36430N
IntActiP0AAM3, 9 interactors
STRINGi316385.ECDH10B_2896

Structurei

Secondary structure

190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 12Combined sources9
Beta strandi14 – 20Combined sources7
Beta strandi22 – 29Combined sources8
Turni31 – 33Combined sources3
Beta strandi47 – 51Combined sources5
Beta strandi54 – 59Combined sources6
Helixi61 – 76Combined sources16
Helixi80 – 85Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OT2NMR-A1-90[»]
ProteinModelPortaliP0AAM3
SMRiP0AAM3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AAM3

Family & Domainsi

Sequence similaritiesi

Belongs to the HupF/HypC family.Curated

Phylogenomic databases

eggNOGiENOG41083WG Bacteria
COG0298 LUCA
HOGENOMiHOG000278755
InParanoidiP0AAM3
KOiK04653
OMAiYVIVHTG
PhylomeDBiP0AAM3

Family and domain databases

Gene3Di2.40.50.970, 1 hit
InterProiView protein in InterPro
IPR037254 HupF/HypC_sf
IPR019812 Hydgase_assmbl_chp_CS
IPR001109 Hydrogenase_HupF/HypC
PANTHERiPTHR35177 PTHR35177, 1 hit
PfamiView protein in Pfam
PF01455 HupF_HypC, 1 hit
PRINTSiPR00445 HUPFHYPC
SUPFAMiSSF159127 SSF159127, 1 hit
TIGRFAMsiTIGR00074 hypC_hupF, 1 hit
PROSITEiView protein in PROSITE
PS01097 HUPF_HYPC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCIGVPGQIR TIDGNQAKVD VCGIQRDVDL TLVGSCDENG QPRVGQWVLV
60 70 80 90
HVGFAMSVIN EAEARDTLDA LQNMFDVEPD VGALLYGEEK
Length:90
Mass (Da):9,732
Last modified:October 11, 2005 - v1
Checksum:iFBE252F1875C7879
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA Translation: CAA38414.1
U29579 Genomic DNA Translation: AAA69238.1
U00096 Genomic DNA Translation: AAC75770.1
AP009048 Genomic DNA Translation: BAE76805.1
PIRiS15199
RefSeqiNP_417208.1, NC_000913.3
WP_000334881.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75770; AAC75770; b2728
BAE76805; BAE76805; BAE76805
GeneIDi947192
KEGGiecj:JW2698
eco:b2728
PATRICifig|1411691.4.peg.4013

Similar proteinsi

Entry informationi

Entry nameiHYPC_ECOLI
AccessioniPrimary (citable) accession number: P0AAM3
Secondary accession number(s): P24191, Q2MAA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health