P0AAM3 (HYPC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydrogenase isoenzymes formation protein HypC

Gene names

Name:	hypC
Ordered Locus Names:	b2728, JW2698

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is required for the formation of all three hydrogenase isoenzymes. May bind to the precursor form of the large subunit of dehydrogenases to keep them in a conformation accessible for metal incorporation. Ref.5
Sequence similarities	Belongs to the HupF/HypC family.

Ontologies

Keywords
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein maturation Inferred from direct assay PubMed 8756471. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 90

90

Hydrogenase isoenzymes formation protein HypC

PRO_0000201398

Secondary structure

1

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90

Helix Strand Turn

Details...

Beta strand

4 – 12

9

Beta strand

14 – 20

7

Beta strand

22 – 29

8

Turn

31 – 33

3

Beta strand

47 – 51

5

Beta strand

54 – 59

6

Helix

61 – 76

16

Helix

80 – 85

6

Sequences

	Sequence		Length	Mass (Da)
	P0AAM3 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: FBE252F1875C7879	FASTA	90	9,732
	10 20 30 40 50 60 MCIGVPGQIR TIDGNQAKVD VCGIQRDVDL TLVGSCDENG QPRVGQWVLV HVGFAMSVIN 70 80 90 EAEARDTLDA LQNMFDVEPD VGALLYGEEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli." Lutz S., Jacobi A., Schlensog V., Boehm R., Sawers G., Boeck A. Mol. Microbiol. 5:123-135(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
[5]	"Interaction of the hydrogenase accessory protein HypC with HycE, the large subunit of Escherichia coli hydrogenase 3 during enzyme maturation." Drapal N., Boeck A. Biochemistry 37:2941-2948(1998) [PubMed] [Europe PMC] [Abstract] Cited for: POSSIBLE FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X54543 Genomic DNA. Translation: CAA38414.1.
U29579 Genomic DNA. Translation: AAA69238.1.
U00096 Genomic DNA. Translation: AAC75770.1.
AP009048 Genomic DNA. Translation: BAE76805.1.

PIR

S15199.

RefSeq

NP_417208.1. NC_000913.2.
YP_490937.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OT2	NMR	-	A	1-90	[»]

ProteinModelPortal

P0AAM3.

SMR

P0AAM3. Positions 1-90.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-36430N.

IntAct

P0AAM3. 8 interactions.

MINT

MINT-1223094.

STRING

511145.b2728.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC75770; AAC75770; b2728.
BAE76805; BAE76805; BAE76805.

GeneID

12932432.
947192.

KEGG

ecj:Y75_p2666.
eco:b2728.

PATRIC

32120856. VBIEscCol129921_2820.

Organism-specific databases

EchoBASE

EB0480.

EcoGene

EG10485. hypC.

Phylogenomic databases

eggNOG

COG0298.

HOGENOM

HOG000278755.

KO

K04653.

OMA

FAMAKID.

ProtClustDB

PRK10409.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10485-MONOMER.
ECOL316407:JW2698-MONOMER.

Gene expression databases

Genevestigator

P0AAM3.

Family and domain databases

InterPro

IPR019812. Hydgase_assmbl_chp_CS.
IPR001109. Hydrogenase_HupF/HypC.
[Graphical view]

Pfam

PF01455. HupF_HypC. 1 hit.
[Graphical view]

PRINTS

PR00445. HUPFHYPC.

TIGRFAMs

TIGR00074. hypC_hupF. 1 hit.

PROSITE

PS01097. HUPF_HYPC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0AAM3.

Entry information

Entry name

HYPC_ECOLI

Accession

Primary (citable) accession number: P0AAM3
Secondary accession number(s): P24191, Q2MAA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program