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Protein

Hydrogenase isoenzymes formation protein HypC

Gene

hypC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Is required for the formation of all three hydrogenase isoenzymes. May bind to the precursor form of the large subunit of dehydrogenases to keep them in a conformation accessible for metal incorporation.

GO - Molecular functioni

  • carbon dioxide binding Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • iron ion binding Source: EcoCyc

GO - Biological processi

  • protein maturation Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG10485-MONOMER.
ECOL316407:JW2698-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase isoenzymes formation protein HypC
Gene namesi
Name:hypC
Ordered Locus Names:b2728, JW2698
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10485. hypC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9090Hydrogenase isoenzymes formation protein HypCPRO_0000201398Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-36430N.
IntActiP0AAM3. 9 interactions.
MINTiMINT-1223094.
STRINGi511145.b2728.

Structurei

Secondary structure

1
90
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 129Combined sources
Beta strandi14 – 207Combined sources
Beta strandi22 – 298Combined sources
Turni31 – 333Combined sources
Beta strandi47 – 515Combined sources
Beta strandi54 – 596Combined sources
Helixi61 – 7616Combined sources
Helixi80 – 856Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OT2NMR-A1-90[»]
ProteinModelPortaliP0AAM3.
SMRiP0AAM3. Positions 1-90.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AAM3.

Family & Domainsi

Sequence similaritiesi

Belongs to the HupF/HypC family.Curated

Phylogenomic databases

eggNOGiCOG0298.
HOGENOMiHOG000278755.
InParanoidiP0AAM3.
KOiK04653.
OMAiQKMGADY.
OrthoDBiEOG6GR3F3.
PhylomeDBiP0AAM3.

Family and domain databases

InterProiIPR019812. Hydgase_assmbl_chp_CS.
IPR001109. Hydrogenase_HupF/HypC.
[Graphical view]
PfamiPF01455. HupF_HypC. 1 hit.
[Graphical view]
PRINTSiPR00445. HUPFHYPC.
TIGRFAMsiTIGR00074. hypC_hupF. 1 hit.
PROSITEiPS01097. HUPF_HYPC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCIGVPGQIR TIDGNQAKVD VCGIQRDVDL TLVGSCDENG QPRVGQWVLV
60 70 80 90
HVGFAMSVIN EAEARDTLDA LQNMFDVEPD VGALLYGEEK
Length:90
Mass (Da):9,732
Last modified:October 11, 2005 - v1
Checksum:iFBE252F1875C7879
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA. Translation: CAA38414.1.
U29579 Genomic DNA. Translation: AAA69238.1.
U00096 Genomic DNA. Translation: AAC75770.1.
AP009048 Genomic DNA. Translation: BAE76805.1.
PIRiS15199.
RefSeqiNP_417208.1. NC_000913.3.
WP_000334881.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC75770; AAC75770; b2728.
BAE76805; BAE76805; BAE76805.
GeneIDi947192.
KEGGieco:b2728.
PATRICi32120856. VBIEscCol129921_2820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA. Translation: CAA38414.1.
U29579 Genomic DNA. Translation: AAA69238.1.
U00096 Genomic DNA. Translation: AAC75770.1.
AP009048 Genomic DNA. Translation: BAE76805.1.
PIRiS15199.
RefSeqiNP_417208.1. NC_000913.3.
WP_000334881.1. NZ_CP010445.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OT2NMR-A1-90[»]
ProteinModelPortaliP0AAM3.
SMRiP0AAM3. Positions 1-90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-36430N.
IntActiP0AAM3. 9 interactions.
MINTiMINT-1223094.
STRINGi511145.b2728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75770; AAC75770; b2728.
BAE76805; BAE76805; BAE76805.
GeneIDi947192.
KEGGieco:b2728.
PATRICi32120856. VBIEscCol129921_2820.

Organism-specific databases

EchoBASEiEB0480.
EcoGeneiEG10485. hypC.

Phylogenomic databases

eggNOGiCOG0298.
HOGENOMiHOG000278755.
InParanoidiP0AAM3.
KOiK04653.
OMAiQKMGADY.
OrthoDBiEOG6GR3F3.
PhylomeDBiP0AAM3.

Enzyme and pathway databases

BioCyciEcoCyc:EG10485-MONOMER.
ECOL316407:JW2698-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AAM3.
PROiP0AAM3.

Family and domain databases

InterProiIPR019812. Hydgase_assmbl_chp_CS.
IPR001109. Hydrogenase_HupF/HypC.
[Graphical view]
PfamiPF01455. HupF_HypC. 1 hit.
[Graphical view]
PRINTSiPR00445. HUPFHYPC.
TIGRFAMsiTIGR00074. hypC_hupF. 1 hit.
PROSITEiPS01097. HUPF_HYPC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli."
    Lutz S., Jacobi A., Schlensog V., Boehm R., Sawers G., Boeck A.
    Mol. Microbiol. 5:123-135(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  5. "Interaction of the hydrogenase accessory protein HypC with HycE, the large subunit of Escherichia coli hydrogenase 3 during enzyme maturation."
    Drapal N., Boeck A.
    Biochemistry 37:2941-2948(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION.

Entry informationi

Entry nameiHYPC_ECOLI
AccessioniPrimary (citable) accession number: P0AAM3
Secondary accession number(s): P24191, Q2MAA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: July 22, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.