Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit

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P0AAM1 (CYBH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 65. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit

Gene names

Name:	hyaC
Ordered Locus Names:	b0974, JW0956

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable b-type cytochrome.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HupC/HyaC/HydC family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	respiratory electron transport chain Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 235

235

Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit

PRO_0000201384

Regions

Topological domain

1 – 19

Cytoplasmic Potential

Transmembrane

20 – 40

Helical; Potential

Topological domain

41 – 63

Periplasmic Potential

Transmembrane

64 – 84

Helical; Potential

Topological domain

85 – 130

Cytoplasmic Potential

Transmembrane

131 – 151

Helical; Potential

Topological domain

152 – 185

Periplasmic Potential

Transmembrane

186 – 203

Helical; Potential

Topological domain

204 – 235

Cytoplasmic Potential

Secondary structure

235

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AAM1 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 98048DC800AB02C7
Show »

FASTA

235

27,597

        10         20         30         40         50         60 
MQQKSDNVVS HYVFEAPVRI WHWLTVLCMA VLMVTGYFIG KPLPSVSGEA TYLFYMGYIR 

        70         80         90        100        110        120 
LIHFSAGMVF TVVLLMRIYW AFVGNRYSRE LFIVPVWRKS WWQGVWYEIR WYLFLAKRPS 

       130        140        150        160        170        180 
ADIGHNPIAQ AAMFGYFLMS VFMIITGFAL YSEHSQYAIF APFRYVVEFF YWTGGNSMDI 

       190        200        210        220        230 
HSWHRLGMWL IGAFVIGHVY MALREDIMSD DTVISTMVNG YRSHKFGKIS NKERS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames." Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E. J. Bacteriol. 172:1969-1977(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M34825 Genomic DNA. Translation: AAA23999.1.
U00096 Genomic DNA. Translation: AAC74059.1.
AP009048 Genomic DNA. Translation: BAA35739.1.

PIR

BVECYC. JV0074.

RefSeq

NP_415493.1. NC_000913.2.
YP_489245.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GD3	X-ray	3.30	A/B	1-235	[»]

ProteinModelPortal

P0AAM1.

SMR

P0AAM1. Positions 8-210.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0AAM1. 1 interaction.

STRING

511145.b0974.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74059; AAC74059; b0974.
BAA35739; BAA35739; BAA35739.

GeneID

12931050.
945581.

KEGG

ecj:Y75_p0945.
eco:b0974.

PATRIC

32117169. VBIEscCol129921_1008.

Organism-specific databases

EchoBASE

EB0465.

EcoGene

EG10470. hyaC.

Phylogenomic databases

eggNOG

COG1969.

HOGENOM

HOG000291339.

K03620.

OMA

REDIMGR.

ProtClustDB

PRK10171.

Enzyme and pathway databases

BioCyc

EcoCyc:HYAC-MONOMER.
ECOL316407:JW0956-MONOMER.
MetaCyc:HYAC-MONOMER.

Gene expression databases

Genevestigator

P0AAM1.

Family and domain databases

InterPro

IPR011577. Cyt_b561_bac/Ni-Hgenase.
IPR016174. Di-haem_cyt_TM.
IPR000516. Ni-dep_Hydgase_cyt-B.
[Graphical view]

Pfam

PF00033. Cytochrom_B_N. 1 hit.
PF01292. Ni_hydr_CYTB. 1 hit.
[Graphical view]

PRINTS

PR00161. NIHGNASECYTB.

SUPFAM

SSF81342. Transmembr_di-haem_cytochrome. 1 hit.

TIGRFAMs

TIGR02125. CytB-hydogenase. 1 hit.

PROSITE

PS00882. NI_HGENASE_CYTB_1. 1 hit.
PS00883. NI_HGENASE_CYTB_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CYBH_ECOLI

Accession

Primary (citable) accession number: P0AAM1
Secondary accession number(s): P19929

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents