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Protein

Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit

Gene

hyaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable b-type cytochrome.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYAC-MONOMER.
ECOL316407:JW0956-MONOMER.
MetaCyc:HYAC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit
Gene namesi
Name:hyaC
Ordered Locus Names:b0974, JW0956
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10470. hyaC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei20 – 4021HelicalSequence AnalysisAdd
BLAST
Topological domaini41 – 6323PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei64 – 8421HelicalSequence AnalysisAdd
BLAST
Topological domaini85 – 13046CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei131 – 15121HelicalSequence AnalysisAdd
BLAST
Topological domaini152 – 18534PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei186 – 20318HelicalSequence AnalysisAdd
BLAST
Topological domaini204 – 23532CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 235235Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunitPRO_0000201384Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-35859N.
IntActiP0AAM1. 1 interaction.
STRINGi511145.b0974.

Structurei

Secondary structure

1
235
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 4021Combined sources
Helixi50 – 523Combined sources
Helixi55 – 8127Combined sources
Turni82 – 854Combined sources
Turni106 – 1083Combined sources
Beta strandi109 – 1135Combined sources
Turni130 – 1323Combined sources
Helixi133 – 15119Combined sources
Turni152 – 1554Combined sources
Helixi161 – 1633Combined sources
Helixi164 – 17310Combined sources
Helixi178 – 20730Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GD3X-ray3.30A/B1-235[»]
ProteinModelPortaliP0AAM1.
SMRiP0AAM1. Positions 8-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HupC/HyaC/HydC family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1969.
HOGENOMiHOG000291339.
InParanoidiP0AAM1.
KOiK03620.
OMAiIITGYKH.
OrthoDBiEOG6B8XHX.
PhylomeDBiP0AAM1.

Family and domain databases

InterProiIPR011577. Cyt_b561_bac/Ni-Hgenase.
IPR016174. Di-haem_cyt_TM.
IPR000516. Ni-dep_Hydgase_cyt-B.
[Graphical view]
PfamiPF00033. Cytochrom_B_N. 1 hit.
PF01292. Ni_hydr_CYTB. 1 hit.
[Graphical view]
PRINTSiPR00161. NIHGNASECYTB.
SUPFAMiSSF81342. SSF81342. 1 hit.
TIGRFAMsiTIGR02125. CytB-hydogenase. 1 hit.
PROSITEiPS00882. NI_HGENASE_CYTB_1. 1 hit.
PS00883. NI_HGENASE_CYTB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQKSDNVVS HYVFEAPVRI WHWLTVLCMA VLMVTGYFIG KPLPSVSGEA
60 70 80 90 100
TYLFYMGYIR LIHFSAGMVF TVVLLMRIYW AFVGNRYSRE LFIVPVWRKS
110 120 130 140 150
WWQGVWYEIR WYLFLAKRPS ADIGHNPIAQ AAMFGYFLMS VFMIITGFAL
160 170 180 190 200
YSEHSQYAIF APFRYVVEFF YWTGGNSMDI HSWHRLGMWL IGAFVIGHVY
210 220 230
MALREDIMSD DTVISTMVNG YRSHKFGKIS NKERS
Length:235
Mass (Da):27,597
Last modified:October 11, 2005 - v1
Checksum:i98048DC800AB02C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34825 Genomic DNA. Translation: AAA23999.1.
U00096 Genomic DNA. Translation: AAC74059.1.
AP009048 Genomic DNA. Translation: BAA35739.1.
PIRiJV0074. BVECYC.
RefSeqiNP_415493.1. NC_000913.3.
WP_001186424.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC74059; AAC74059; b0974.
BAA35739; BAA35739; BAA35739.
GeneIDi945581.
KEGGieco:b0974.
PATRICi32117169. VBIEscCol129921_1008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34825 Genomic DNA. Translation: AAA23999.1.
U00096 Genomic DNA. Translation: AAC74059.1.
AP009048 Genomic DNA. Translation: BAA35739.1.
PIRiJV0074. BVECYC.
RefSeqiNP_415493.1. NC_000913.3.
WP_001186424.1. NZ_CP010445.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GD3X-ray3.30A/B1-235[»]
ProteinModelPortaliP0AAM1.
SMRiP0AAM1. Positions 8-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-35859N.
IntActiP0AAM1. 1 interaction.
STRINGi511145.b0974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74059; AAC74059; b0974.
BAA35739; BAA35739; BAA35739.
GeneIDi945581.
KEGGieco:b0974.
PATRICi32117169. VBIEscCol129921_1008.

Organism-specific databases

EchoBASEiEB0465.
EcoGeneiEG10470. hyaC.

Phylogenomic databases

eggNOGiCOG1969.
HOGENOMiHOG000291339.
InParanoidiP0AAM1.
KOiK03620.
OMAiIITGYKH.
OrthoDBiEOG6B8XHX.
PhylomeDBiP0AAM1.

Enzyme and pathway databases

BioCyciEcoCyc:HYAC-MONOMER.
ECOL316407:JW0956-MONOMER.
MetaCyc:HYAC-MONOMER.

Miscellaneous databases

PROiP0AAM1.

Family and domain databases

InterProiIPR011577. Cyt_b561_bac/Ni-Hgenase.
IPR016174. Di-haem_cyt_TM.
IPR000516. Ni-dep_Hydgase_cyt-B.
[Graphical view]
PfamiPF00033. Cytochrom_B_N. 1 hit.
PF01292. Ni_hydr_CYTB. 1 hit.
[Graphical view]
PRINTSiPR00161. NIHGNASECYTB.
SUPFAMiSSF81342. SSF81342. 1 hit.
TIGRFAMsiTIGR02125. CytB-hydogenase. 1 hit.
PROSITEiPS00882. NI_HGENASE_CYTB_1. 1 hit.
PS00883. NI_HGENASE_CYTB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames."
    Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E.
    J. Bacteriol. 172:1969-1977(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCYBH_ECOLI
AccessioniPrimary (citable) accession number: P0AAM1
Secondary accession number(s): P19929
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: July 22, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.