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Protein

Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit

Gene

hyaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable b-type cytochrome.

GO - Molecular functioni

  • electron carrier activity Source: InterPro
  • heme binding Source: EcoCyc
  • iron ion binding Source: InterPro

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • fermentation Source: EcoCyc
  • respiratory electron transport chain Source: InterPro
  • transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYAC-MONOMER.
ECOL316407:JW0956-MONOMER.
MetaCyc:HYAC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit
Gene namesi
Name:hyaC
Ordered Locus Names:b0974, JW0956
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10470. hyaC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19CytoplasmicSequence analysisAdd BLAST19
Transmembranei20 – 40HelicalSequence analysisAdd BLAST21
Topological domaini41 – 63PeriplasmicSequence analysisAdd BLAST23
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Topological domaini85 – 130CytoplasmicSequence analysisAdd BLAST46
Transmembranei131 – 151HelicalSequence analysisAdd BLAST21
Topological domaini152 – 185PeriplasmicSequence analysisAdd BLAST34
Transmembranei186 – 203HelicalSequence analysisAdd BLAST18
Topological domaini204 – 235CytoplasmicSequence analysisAdd BLAST32

GO - Cellular componenti

  • [Ni-Fe] hydrogenase complex Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002013841 – 235Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunitAdd BLAST235

Proteomic databases

PaxDbiP0AAM1.
PRIDEiP0AAM1.

Interactioni

Protein-protein interaction databases

BioGridi4260044. 6 interactors.
DIPiDIP-35859N.
IntActiP0AAM1. 1 interactor.
STRINGi511145.b0974.

Structurei

Secondary structure

1235
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 40Combined sources21
Helixi50 – 52Combined sources3
Helixi55 – 81Combined sources27
Turni82 – 85Combined sources4
Turni106 – 108Combined sources3
Beta strandi109 – 113Combined sources5
Turni130 – 132Combined sources3
Helixi133 – 151Combined sources19
Turni152 – 155Combined sources4
Helixi161 – 163Combined sources3
Helixi164 – 173Combined sources10
Helixi178 – 207Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GD3X-ray3.30A/B1-235[»]
ProteinModelPortaliP0AAM1.
SMRiP0AAM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HupC/HyaC/HydC family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105X7T. Bacteria.
COG1969. LUCA.
HOGENOMiHOG000291339.
InParanoidiP0AAM1.
KOiK03620.
OMAiIITGYKH.
PhylomeDBiP0AAM1.

Family and domain databases

InterProiIPR011577. Cyt_b561_bac/Ni-Hgenase.
IPR016174. Di-haem_cyt_TM.
IPR000516. Ni-dep_Hydgase_cyt-B.
[Graphical view]
PfamiPF01292. Ni_hydr_CYTB. 1 hit.
[Graphical view]
PRINTSiPR00161. NIHGNASECYTB.
SUPFAMiSSF81342. SSF81342. 1 hit.
TIGRFAMsiTIGR02125. CytB-hydogenase. 1 hit.
PROSITEiPS00882. NI_HGENASE_CYTB_1. 1 hit.
PS00883. NI_HGENASE_CYTB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQKSDNVVS HYVFEAPVRI WHWLTVLCMA VLMVTGYFIG KPLPSVSGEA
60 70 80 90 100
TYLFYMGYIR LIHFSAGMVF TVVLLMRIYW AFVGNRYSRE LFIVPVWRKS
110 120 130 140 150
WWQGVWYEIR WYLFLAKRPS ADIGHNPIAQ AAMFGYFLMS VFMIITGFAL
160 170 180 190 200
YSEHSQYAIF APFRYVVEFF YWTGGNSMDI HSWHRLGMWL IGAFVIGHVY
210 220 230
MALREDIMSD DTVISTMVNG YRSHKFGKIS NKERS
Length:235
Mass (Da):27,597
Last modified:October 11, 2005 - v1
Checksum:i98048DC800AB02C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34825 Genomic DNA. Translation: AAA23999.1.
U00096 Genomic DNA. Translation: AAC74059.1.
AP009048 Genomic DNA. Translation: BAA35739.1.
PIRiJV0074. BVECYC.
RefSeqiNP_415493.1. NC_000913.3.
WP_001186424.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74059; AAC74059; b0974.
BAA35739; BAA35739; BAA35739.
GeneIDi945581.
KEGGiecj:JW0956.
eco:b0974.
PATRICi32117169. VBIEscCol129921_1008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34825 Genomic DNA. Translation: AAA23999.1.
U00096 Genomic DNA. Translation: AAC74059.1.
AP009048 Genomic DNA. Translation: BAA35739.1.
PIRiJV0074. BVECYC.
RefSeqiNP_415493.1. NC_000913.3.
WP_001186424.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GD3X-ray3.30A/B1-235[»]
ProteinModelPortaliP0AAM1.
SMRiP0AAM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260044. 6 interactors.
DIPiDIP-35859N.
IntActiP0AAM1. 1 interactor.
STRINGi511145.b0974.

Proteomic databases

PaxDbiP0AAM1.
PRIDEiP0AAM1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74059; AAC74059; b0974.
BAA35739; BAA35739; BAA35739.
GeneIDi945581.
KEGGiecj:JW0956.
eco:b0974.
PATRICi32117169. VBIEscCol129921_1008.

Organism-specific databases

EchoBASEiEB0465.
EcoGeneiEG10470. hyaC.

Phylogenomic databases

eggNOGiENOG4105X7T. Bacteria.
COG1969. LUCA.
HOGENOMiHOG000291339.
InParanoidiP0AAM1.
KOiK03620.
OMAiIITGYKH.
PhylomeDBiP0AAM1.

Enzyme and pathway databases

BioCyciEcoCyc:HYAC-MONOMER.
ECOL316407:JW0956-MONOMER.
MetaCyc:HYAC-MONOMER.

Miscellaneous databases

PROiP0AAM1.

Family and domain databases

InterProiIPR011577. Cyt_b561_bac/Ni-Hgenase.
IPR016174. Di-haem_cyt_TM.
IPR000516. Ni-dep_Hydgase_cyt-B.
[Graphical view]
PfamiPF01292. Ni_hydr_CYTB. 1 hit.
[Graphical view]
PRINTSiPR00161. NIHGNASECYTB.
SUPFAMiSSF81342. SSF81342. 1 hit.
TIGRFAMsiTIGR02125. CytB-hydogenase. 1 hit.
PROSITEiPS00882. NI_HGENASE_CYTB_1. 1 hit.
PS00883. NI_HGENASE_CYTB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYBH_ECOLI
AccessioniPrimary (citable) accession number: P0AAM1
Secondary accession number(s): P19929
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.