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P0AAM1 (CYBH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit
Gene names
Name:hyaC
Ordered Locus Names:b0974, JW0956
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable b-type cytochrome.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the HupC/HyaC/HydC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 235235Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit
PRO_0000201384

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 4021Helical; Potential
Topological domain41 – 6323Periplasmic Potential
Transmembrane64 – 8421Helical; Potential
Topological domain85 – 13046Cytoplasmic Potential
Transmembrane131 – 15121Helical; Potential
Topological domain152 – 18534Periplasmic Potential
Transmembrane186 – 20318Helical; Potential
Topological domain204 – 23532Cytoplasmic Potential

Secondary structure

.................... 235
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AAM1 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 98048DC800AB02C7

FASTA23527,597
        10         20         30         40         50         60 
MQQKSDNVVS HYVFEAPVRI WHWLTVLCMA VLMVTGYFIG KPLPSVSGEA TYLFYMGYIR 

        70         80         90        100        110        120 
LIHFSAGMVF TVVLLMRIYW AFVGNRYSRE LFIVPVWRKS WWQGVWYEIR WYLFLAKRPS 

       130        140        150        160        170        180 
ADIGHNPIAQ AAMFGYFLMS VFMIITGFAL YSEHSQYAIF APFRYVVEFF YWTGGNSMDI 

       190        200        210        220        230 
HSWHRLGMWL IGAFVIGHVY MALREDIMSD DTVISTMVNG YRSHKFGKIS NKERS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames."
Menon N.K., Robbins J., Peck H.D. Jr., Chatelus C.Y., Choi E.-S., Przybyla A.E.
J. Bacteriol. 172:1969-1977(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34825 Genomic DNA. Translation: AAA23999.1.
U00096 Genomic DNA. Translation: AAC74059.1.
AP009048 Genomic DNA. Translation: BAA35739.1.
PIRBVECYC. JV0074.
RefSeqNP_415493.1. NC_000913.3.
YP_489245.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4GD3X-ray3.30A/B1-235[»]
ProteinModelPortalP0AAM1.
SMRP0AAM1. Positions 8-210.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35859N.
IntActP0AAM1. 1 interaction.
STRING511145.b0974.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74059; AAC74059; b0974.
BAA35739; BAA35739; BAA35739.
GeneID12931050.
945581.
KEGGecj:Y75_p0945.
eco:b0974.
PATRIC32117169. VBIEscCol129921_1008.

Organism-specific databases

EchoBASEEB0465.
EcoGeneEG10470. hyaC.

Phylogenomic databases

eggNOGCOG1969.
HOGENOMHOG000291339.
KOK03620.
OMAIITGYKH.
OrthoDBEOG6B8XHX.
PhylomeDBP0AAM1.
ProtClustDBPRK10171.

Enzyme and pathway databases

BioCycEcoCyc:HYAC-MONOMER.
ECOL316407:JW0956-MONOMER.
MetaCyc:HYAC-MONOMER.

Gene expression databases

GenevestigatorP0AAM1.

Family and domain databases

InterProIPR011577. Cyt_b561_bac/Ni-Hgenase.
IPR016174. Di-haem_cyt_TM.
IPR000516. Ni-dep_Hydgase_cyt-B.
[Graphical view]
PfamPF00033. Cytochrom_B_N. 1 hit.
PF01292. Ni_hydr_CYTB. 1 hit.
[Graphical view]
PRINTSPR00161. NIHGNASECYTB.
SUPFAMSSF81342. SSF81342. 1 hit.
TIGRFAMsTIGR02125. CytB-hydogenase. 1 hit.
PROSITEPS00882. NI_HGENASE_CYTB_1. 1 hit.
PS00883. NI_HGENASE_CYTB_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0AAM1.

Entry information

Entry nameCYBH_ECOLI
AccessionPrimary (citable) accession number: P0AAM1
Secondary accession number(s): P19929
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene