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Protein

Ferredoxin-type protein NapG

Gene

napG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for electron transfer from ubiquinol, via NapC, to the periplasmic nitrate reductase NapAB complex.2 Publications

Cofactori

[4Fe-4S] clusterPROSITE-ProRule annotationNote: Binds 4 [4Fe-4S] cluster.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi64Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi67Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi71Iron-sulfur 1 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi99Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi102Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi107Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi111Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi139Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi147Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi150Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi154Iron-sulfur 3 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi186Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi189Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi192Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi196Iron-sulfur 4 (4Fe-4S)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:NAPG-MONOMER
MetaCyc:NAPG-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin-type protein NapGCurated
Alternative name(s):
Ubiquinol--[NapC cytochrome c] reductase NapG subunitCurated
Gene namesi
Name:napG
Synonyms:yojA, yojB
Ordered Locus Names:b2205, JW2193
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12064 napG

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Deletion of both napG and napH genes has little effect on the overall rate of nitrate reduction during growth in the glycerol/nitrate medium. However, during growth in the glucose/nitrate medium, the double mutant shows a decreased rate of electron transfer that correlates with decreased NapAB activity.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi11 – 12RR → AA: Cannot complement a deletion mutant. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 41Tat-type signalPROSITE-ProRule annotationAdd BLAST41
ChainiPRO_000015927942 – 231Ferredoxin-type protein NapGAdd BLAST190

Post-translational modificationi

Exported by the Tat system (PubMed:17218314). The position of the signal peptide cleavage has not been experimentally proven.Curated1 Publication

Proteomic databases

PaxDbiP0AAL3
PRIDEiP0AAL3

Interactioni

Protein-protein interaction databases

BioGridi4262222, 16 interactors
IntActiP0AAL3, 4 interactors
STRINGi316385.ECDH10B_2362

Structurei

3D structure databases

ProteinModelPortaliP0AAL3
SMRiP0AAL3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 814Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST32
Domaini89 – 1214Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST33
Domaini130 – 1664Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST37
Domaini177 – 2084Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4108BGM Bacteria
COG1145 LUCA
HOGENOMiHOG000275499
InParanoidiP0AAL3
KOiK02573
OMAiLIDHETC
PhylomeDBiP0AAL3

Family and domain databases

InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR004494 MauM_NapG
IPR006311 TAT_signal
PfamiView protein in Pfam
PF12800 Fer4_4, 2 hits
PF12838 Fer4_7, 1 hit
TIGRFAMsiTIGR00397 mauM_napG, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 4 hits
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRSAKPQNG RRRFLRDVVR TAGGLAAVGV ALGLQQQTAR ASGVRLRPPG
60 70 80 90 100
AINENAFASA CVRCGQCVQA CPYDTLKLAT LASGLSAGTP YFVARDIPCE
110 120 130 140 150
MCEDIPCAKV CPSGALDREI ESIDDARMGL AVLVDQENCL NFQGLRCDVC
160 170 180 190 200
YRECPKIDEA ITLELERNTR TGKHARFLPT VHSDACTGCG KCEKVCVLEQ
210 220 230
PAIKVLPLSL AKGELGHHYR FGWLEGNNGK S
Length:231
Mass (Da):24,925
Last modified:October 11, 2005 - v1
Checksum:i9DA55774D6A68AD5
GO

Sequence cautioni

The sequence AAA16397 differs from that shown. Reason: Frameshift at position 145.Curated
The sequence AAA16398 differs from that shown. Reason: Frameshift at position 145.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA Translation: AAA16398.1 Frameshift.
U00008 Genomic DNA Translation: AAA16397.1 Frameshift.
U00096 Genomic DNA Translation: AAC75265.1
AP009048 Genomic DNA Translation: BAE76668.1
PIRiC64990
RefSeqiNP_416709.1, NC_000913.3
WP_000091291.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75265; AAC75265; b2205
BAE76668; BAE76668; BAE76668
GeneIDi945544
KEGGiecj:JW2193
eco:b2205
PATRICifig|1411691.4.peg.31

Similar proteinsi

Entry informationi

Entry nameiNAPG_ECOLI
AccessioniPrimary (citable) accession number: P0AAL3
Secondary accession number(s): P33935, P33936, Q2MAN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health