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Protein

Ferredoxin-type protein NapF

Gene

napF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could be involved in the maturation of NapA, the catalytic subunit of the periplasmic nitrate reductase, before its export into the periplasm (PubMed:17074894). Is not involved in the electron transfer from menaquinol or ubiquinol to the periplasmic nitrate reductase (PubMed:11967083).2 Publications

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 3 [4Fe-4S] cluster.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi40Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi43Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi47Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi69Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi72Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi75Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi79Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi141Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi144Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi147Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi151Iron-sulfur 3 (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • response to oxidative stress Source: EcoCyc

Keywordsi

Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:NAPF-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ferredoxin-type protein NapFUniRule annotationCurated
Gene namesi
Name:napFUniRule annotation
Synonyms:yojG
Ordered Locus Names:b2208, JW2196
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12068 napF

Subcellular locationi

  • Cytoplasm 1 Publication
  • Note: Loosely attached to the inner side of the membrane.1 Publication

GO - Cellular componenti

  • cytoplasm Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene does not decrease the rate of electron transfer from physiological substrates to the Nap complex, but it has a significant effect on cell energetics.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7 – 8RR → AA: No change in activity. 1 Publication2
Mutagenesisi24P → A: No change in activity. 1 Publication1
Mutagenesisi25P → A: No change in activity. 1 Publication1
Mutagenesisi26W → Y: No change in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001592751 – 164Ferredoxin-type protein NapFAdd BLAST164

Proteomic databases

PaxDbiP0AAL0
PRIDEiP0AAL0

Interactioni

Subunit structurei

Interacts with the cytoplasmic NapA precursor.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4262219, 35 interactors
STRINGi316385.ECDH10B_2365

Structurei

3D structure databases

ProteinModelPortaliP0AAL0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 574Fe-4S ferredoxin-type 1UniRule annotationAdd BLAST30
Domaini58 – 894Fe-4S ferredoxin-type 2UniRule annotationAdd BLAST32
Domaini132 – 1614Fe-4S ferredoxin-type 3UniRule annotationAdd BLAST30

Sequence similaritiesi

Belongs to the NapF family.UniRule annotationCurated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108ZNS Bacteria
COG1145 LUCA
HOGENOMiHOG000275477
InParanoidiP0AAL0
KOiK02572
OMAiSCKDACD
PhylomeDBiP0AAL0

Family and domain databases

CDDicd10564 NapF_like, 1 hit
HAMAPiMF_02201 NapF, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR004496 NapF
PANTHERiPTHR24960:SF46 PTHR24960:SF46, 1 hit
PfamiView protein in Pfam
PF00037 Fer4, 1 hit
PF12800 Fer4_4, 1 hit
PF13187 Fer4_9, 1 hit
TIGRFAMsiTIGR00402 napF, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 3 hits
PS51379 4FE4S_FER_2, 3 hits

Sequencei

Sequence statusi: Complete.

P0AAL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIDASRRGI LTGRWRKASN GIRPPWSGDE SHFLTHCTRC DACINACENN
60 70 80 90 100
ILQRGAGGYP SVNFKNNECS FCYACAQACP ESLFSPRHTR AWDLQFTIGD
110 120 130 140 150
ACLAYQSVEC RRCQDSCEPM AIIFRPTLSG IYQPQLNSQL CNGCGACAAS
160
CPVSAITAEY LHAH
Length:164
Mass (Da):18,047
Last modified:October 11, 2005 - v1
Checksum:i3832B3D78805FAC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00008 Genomic DNA Translation: AAA16401.1
U00096 Genomic DNA Translation: AAC75268.1
AP009048 Genomic DNA Translation: BAA15991.1
PIRiF64990
RefSeqiNP_416712.1, NC_000913.3
WP_000686723.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75268; AAC75268; b2208
BAA15991; BAA15991; BAA15991
GeneIDi946813
KEGGiecj:JW2196
eco:b2208
PATRICifig|1411691.4.peg.28

Similar proteinsi

Entry informationi

Entry nameiNAPF_ECOLI
AccessioniPrimary (citable) accession number: P0AAL0
Secondary accession number(s): P33939
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health