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Protein

Protein NrfC

Gene

nrfC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi49 – 491Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi52 – 521Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi56 – 561Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi92 – 921Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi95 – 951Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi100 – 1001Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi104 – 1041Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi125 – 1251Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi128 – 1281Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi131 – 1311Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi135 – 1351Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi168 – 1681Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi172 – 1721Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • nitrite reductase (cytochrome, ammonia-forming) activity Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:NRFC-MONOMER.
ECOL316407:JW4033-MONOMER.
MetaCyc:NRFC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NrfC
Gene namesi
Name:nrfC
Synonyms:yjcJ
Ordered Locus Names:b4072, JW4033
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11946. nrfC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini28 – 223196Protein NrfCPRO_0000042276Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PaxDbiP0AAK7.

Interactioni

Protein-protein interaction databases

BioGridi4263519. 7 interactions.
IntActiP0AAK7. 3 interactions.
STRINGi511145.b4072.

Structurei

3D structure databases

ProteinModelPortaliP0AAK7.
SMRiP0AAK7. Positions 36-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 65294Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini83 – 114324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini116 – 145304Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105D3S. Bacteria.
COG0437. LUCA.
HOGENOMiHOG000163388.
InParanoidiP0AAK7.
KOiK04014.
OMAiKRYAMVH.
OrthoDBiEOG6JTC8Q.
PhylomeDBiP0AAK7.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017567. Cyt_c_NO2Rdtase_NrfC.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF00037. Fer4. 1 hit.
PF13247. Fer4_11. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03149. cyt_nit_nrfC. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 3 hits.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTWSRRQFLT GVGVLAAVSG TAGRVVAKTL NINGVRYGMV HDESLCIGCT
60 70 80 90 100
ACMDACREVN KVPEGVSRLT IIRSEPQGEF PDVKYRFFRK SCQHCDHAPC
110 120 130 140 150
VDVCPTGASF RDAASGIVDV NPDLCVGCQY CIAACPYRVR FIHPVTKTAD
160 170 180 190 200
KCDFCRKTNL QAGKLPACVE ACPTKALTFG NLDDPNSEIS QLLRQKPTYR
210 220
YKLALGTKPK LYRVPFKYGE VSQ
Length:223
Mass (Da):24,567
Last modified:October 11, 2005 - v1
Checksum:i676C3264D341FC8E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 662GV → AS in AAC43166 (PubMed:8265357).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA. Translation: CAA51043.1.
U00006 Genomic DNA. Translation: AAC43166.1.
U00096 Genomic DNA. Translation: AAC77042.1.
AP009048 Genomic DNA. Translation: BAE78074.1.
PIRiC57987.
RefSeqiNP_418496.1. NC_000913.3.
WP_000220281.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77042; AAC77042; b4072.
BAE78074; BAE78074; BAE78074.
GeneIDi948581.
KEGGiecj:JW4033.
eco:b4072.
PATRICi32123693. VBIEscCol129921_4195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA. Translation: CAA51043.1.
U00006 Genomic DNA. Translation: AAC43166.1.
U00096 Genomic DNA. Translation: AAC77042.1.
AP009048 Genomic DNA. Translation: BAE78074.1.
PIRiC57987.
RefSeqiNP_418496.1. NC_000913.3.
WP_000220281.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAK7.
SMRiP0AAK7. Positions 36-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263519. 7 interactions.
IntActiP0AAK7. 3 interactions.
STRINGi511145.b4072.

Proteomic databases

PaxDbiP0AAK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77042; AAC77042; b4072.
BAE78074; BAE78074; BAE78074.
GeneIDi948581.
KEGGiecj:JW4033.
eco:b4072.
PATRICi32123693. VBIEscCol129921_4195.

Organism-specific databases

EchoBASEiEB1889.
EcoGeneiEG11946. nrfC.

Phylogenomic databases

eggNOGiENOG4105D3S. Bacteria.
COG0437. LUCA.
HOGENOMiHOG000163388.
InParanoidiP0AAK7.
KOiK04014.
OMAiKRYAMVH.
OrthoDBiEOG6JTC8Q.
PhylomeDBiP0AAK7.

Enzyme and pathway databases

BioCyciEcoCyc:NRFC-MONOMER.
ECOL316407:JW4033-MONOMER.
MetaCyc:NRFC-MONOMER.

Miscellaneous databases

PROiP0AAK7.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017567. Cyt_c_NO2Rdtase_NrfC.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF00037. Fer4. 1 hit.
PF13247. Fer4_11. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03149. cyt_nit_nrfC. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 3 hits.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A seven-gene operon essential for formate-dependent nitrite reduction to ammonia by enteric bacteria."
    Hussain H.A., Grove J., Griffiths L., Busby S., Cole J.
    Mol. Microbiol. 12:153-163(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 65-66.
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
    Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
    J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPORT VIA THE TAT-SYSTEM.

Entry informationi

Entry nameiNRFC_ECOLI
AccessioniPrimary (citable) accession number: P0AAK7
Secondary accession number(s): P32708, Q2M6N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: May 11, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.