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Protein

Formate hydrogenlyase subunit 2

Gene

hycB

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Probable electron transfer protein for hydrogenase 3.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi15Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi18Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi22Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi51Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi54Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi59Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi63Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi82Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi85Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi88Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi92Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi143Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi146Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi159Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate hydrogenlyase subunit 2
Short name:
FHL subunit 2
Alternative name(s):
Hydrogenase-3 component B
Gene namesi
Name:hycB
Ordered Locus Names:SF2741, S2933
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001592651 – 203Formate hydrogenlyase subunit 2Add BLAST203

Proteomic databases

PaxDbiP0AAK3.
PRIDEiP0AAK3.

Interactioni

Subunit structurei

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway, molecular hydrogen and carbodioxide are released from formate (By similarity).By similarity

Protein-protein interaction databases

STRINGi198214.SF2741.

Structurei

3D structure databases

ProteinModelPortaliP0AAK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 324Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST31
Domaini42 – 724Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST31
Domaini73 – 1024Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30
Domaini137 – 1694Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Contains 4 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108MFN. Bacteria.
COG1142. LUCA.
HOGENOMiHOG000163385.
KOiK15827.
OMAiRTCPTKA.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRFVIADST LCIGCHTCEA ACSETHRQHG LQSMPRLRVM LNEKESAPQL
60 70 80 90 100
CHHCEDAPCA VVCPVNAITR VDGAVQLNES LCVSCKLCGI ACPFGAIEFS
110 120 130 140 150
GSRPLDIPAN ANTPKAPPAP PAPARVSTLL DWVPGIRAIA VKCDLCSFDE
160 170 180 190 200
QGPACVRMCP TKALHLVDNT DIARVSKRKR ELTFNTDFGD LTLFQQAQSG

EAK
Length:203
Mass (Da):21,873
Last modified:October 11, 2005 - v1
Checksum:i828042E504B00DBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44232.1.
AE014073 Genomic DNA. Translation: AAP18059.1.
RefSeqiNP_708525.1. NC_004337.2.
WP_001079186.1. NZ_LVJC01000019.1.

Genome annotation databases

EnsemblBacteriaiAAN44232; AAN44232; SF2741.
AAP18059; AAP18059; S2933.
GeneIDi1025711.
KEGGisfl:SF2741.
sft:NCTC1_03017.
sfx:S2933.
PATRICi18707531. VBIShiFle31049_3221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44232.1.
AE014073 Genomic DNA. Translation: AAP18059.1.
RefSeqiNP_708525.1. NC_004337.2.
WP_001079186.1. NZ_LVJC01000019.1.

3D structure databases

ProteinModelPortaliP0AAK3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF2741.

Protein family/group databases

TCDBi3.D.1.9.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP0AAK3.
PRIDEiP0AAK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN44232; AAN44232; SF2741.
AAP18059; AAP18059; S2933.
GeneIDi1025711.
KEGGisfl:SF2741.
sft:NCTC1_03017.
sfx:S2933.
PATRICi18707531. VBIShiFle31049_3221.

Phylogenomic databases

eggNOGiENOG4108MFN. Bacteria.
COG1142. LUCA.
HOGENOMiHOG000163385.
KOiK15827.
OMAiRTCPTKA.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYCB_SHIFL
AccessioniPrimary (citable) accession number: P0AAK3
Secondary accession number(s): P16428, Q46883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.