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Protein

Formate hydrogenlyase subunit 2

Gene

hycB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Probable electron transfer protein for hydrogenase 3.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi15Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi18Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi22Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi51Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi54Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi59Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi63Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi82Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi85Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi88Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi92Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi143Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi146Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi159Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYCBSMALL-MONOMER
MetaCyc:HYCBSMALL-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Formate hydrogenlyase subunit 2
Short name:
FHL subunit 2
Alternative name(s):
Hydrogenase-3 component B
Gene namesi
Name:hycB
Synonyms:hevB
Ordered Locus Names:b2724, JW2694
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10475 hycB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001592631 – 203Formate hydrogenlyase subunit 2Add BLAST203

Proteomic databases

PaxDbiP0AAK1
PRIDEiP0AAK1

Interactioni

Subunit structurei

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway, molecular hydrogen and carbodioxide are released from formate.

Protein-protein interaction databases

BioGridi4262103, 9 interactors
IntActiP0AAK1, 4 interactors
MINTiP0AAK1
STRINGi316385.ECDH10B_2892

Structurei

3D structure databases

ProteinModelPortaliP0AAK1
SMRiP0AAK1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 324Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST31
Domaini42 – 724Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST31
Domaini73 – 1024Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30
Domaini137 – 1694Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST33

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108MFN Bacteria
COG1142 LUCA
HOGENOMiHOG000163385
InParanoidiP0AAK1
KOiK15827
OMAiRTCPTKA
PhylomeDBiP0AAK1

Family and domain databases

InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
PfamiView protein in Pfam
PF13247 Fer4_11, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 4 hits

Sequencei

Sequence statusi: Complete.

P0AAK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRFVIADST LCIGCHTCEA ACSETHRQHG LQSMPRLRVM LNEKESAPQL
60 70 80 90 100
CHHCEDAPCA VVCPVNAITR VDGAVQLNES LCVSCKLCGI ACPFGAIEFS
110 120 130 140 150
GSRPLDIPAN ANTPKAPPAP PAPARVSTLL DWVPGIRAIA VKCDLCSFDE
160 170 180 190 200
QGPACVRMCP TKALHLVDNT DIARVSKRKR ELTFNTDFGD LTLFQQAQSG

EAK
Length:203
Mass (Da):21,873
Last modified:October 11, 2005 - v1
Checksum:i828042E504B00DBD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156V → A in CAA35547 (PubMed:2187144).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA Translation: CAA35547.1
U29579 Genomic DNA Translation: AAA69234.1
U00096 Genomic DNA Translation: AAC75766.1
AP009048 Genomic DNA Translation: BAE76801.1
PIRiH65052
RefSeqiNP_417204.1, NC_000913.3
WP_001079186.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75766; AAC75766; b2724
BAE76801; BAE76801; BAE76801
GeneIDi948002
KEGGiecj:JW2694
eco:b2724
PATRICifig|1411691.4.peg.4017

Similar proteinsi

Entry informationi

Entry nameiHYCB_ECOLI
AccessioniPrimary (citable) accession number: P0AAK1
Secondary accession number(s): P16428, Q2MAA5, Q46883
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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