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Protein

Formate hydrogenlyase subunit 2

Gene

hycB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probable electron transfer protein for hydrogenase 3.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi15Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi18Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi22Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi51Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi54Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi59Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi63Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi82Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi85Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi88Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi92Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi143Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi146Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi159Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYCBSMALL-MONOMER.
ECOL316407:JW2694-MONOMER.
MetaCyc:HYCBSMALL-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate hydrogenlyase subunit 2
Short name:
FHL subunit 2
Alternative name(s):
Hydrogenase-3 component B
Gene namesi
Name:hycB
Synonyms:hevB
Ordered Locus Names:b2724, JW2694
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10475. hycB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001592631 – 203Formate hydrogenlyase subunit 2Add BLAST203

Proteomic databases

PaxDbiP0AAK1.
PRIDEiP0AAK1.

Interactioni

Subunit structurei

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway, molecular hydrogen and carbodioxide are released from formate.

Protein-protein interaction databases

BioGridi4262103. 6 interactors.
IntActiP0AAK1. 4 interactors.
STRINGi511145.b2724.

Structurei

3D structure databases

ProteinModelPortaliP0AAK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 324Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST31
Domaini42 – 724Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST31
Domaini73 – 1024Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30
Domaini137 – 1694Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Contains 4 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108MFN. Bacteria.
COG1142. LUCA.
HOGENOMiHOG000163385.
InParanoidiP0AAK1.
KOiK15827.
OMAiRTCPTKA.
PhylomeDBiP0AAK1.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRFVIADST LCIGCHTCEA ACSETHRQHG LQSMPRLRVM LNEKESAPQL
60 70 80 90 100
CHHCEDAPCA VVCPVNAITR VDGAVQLNES LCVSCKLCGI ACPFGAIEFS
110 120 130 140 150
GSRPLDIPAN ANTPKAPPAP PAPARVSTLL DWVPGIRAIA VKCDLCSFDE
160 170 180 190 200
QGPACVRMCP TKALHLVDNT DIARVSKRKR ELTFNTDFGD LTLFQQAQSG

EAK
Length:203
Mass (Da):21,873
Last modified:October 11, 2005 - v1
Checksum:i828042E504B00DBD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156V → A in CAA35547 (PubMed:2187144).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35547.1.
U29579 Genomic DNA. Translation: AAA69234.1.
U00096 Genomic DNA. Translation: AAC75766.1.
AP009048 Genomic DNA. Translation: BAE76801.1.
PIRiH65052.
RefSeqiNP_417204.1. NC_000913.3.
WP_001079186.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75766; AAC75766; b2724.
BAE76801; BAE76801; BAE76801.
GeneIDi948002.
KEGGiecj:JW2694.
eco:b2724.
PATRICi32120848. VBIEscCol129921_2816.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA. Translation: CAA35547.1.
U29579 Genomic DNA. Translation: AAA69234.1.
U00096 Genomic DNA. Translation: AAC75766.1.
AP009048 Genomic DNA. Translation: BAE76801.1.
PIRiH65052.
RefSeqiNP_417204.1. NC_000913.3.
WP_001079186.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAK1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262103. 6 interactors.
IntActiP0AAK1. 4 interactors.
STRINGi511145.b2724.

Proteomic databases

PaxDbiP0AAK1.
PRIDEiP0AAK1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75766; AAC75766; b2724.
BAE76801; BAE76801; BAE76801.
GeneIDi948002.
KEGGiecj:JW2694.
eco:b2724.
PATRICi32120848. VBIEscCol129921_2816.

Organism-specific databases

EchoBASEiEB0470.
EcoGeneiEG10475. hycB.

Phylogenomic databases

eggNOGiENOG4108MFN. Bacteria.
COG1142. LUCA.
HOGENOMiHOG000163385.
InParanoidiP0AAK1.
KOiK15827.
OMAiRTCPTKA.
PhylomeDBiP0AAK1.

Enzyme and pathway databases

BioCyciEcoCyc:HYCBSMALL-MONOMER.
ECOL316407:JW2694-MONOMER.
MetaCyc:HYCBSMALL-MONOMER.

Miscellaneous databases

PROiP0AAK1.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYCB_ECOLI
AccessioniPrimary (citable) accession number: P0AAK1
Secondary accession number(s): P16428, Q2MAA5, Q46883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.