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Protein

Hydrogenase-2 operon protein HybA

Gene

hybA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover.

Caution

Was originally thought to be the small subunit of hydrogenase 2.1 Publication

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi50Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi53Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi57Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi112Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi115Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi120Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi124Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi145Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi148Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi151Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi174Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi177Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi193Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi197Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • anaerobic glycerol catabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYBA-MONOMER
MetaCyc:HYBA-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-2 operon protein HybA
Gene namesi
Name:hybA
Ordered Locus Names:b2996, JW2964
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11799 hybA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Tat-type signalSequence analysisAdd BLAST27
ChainiPRO_000004227328 – 328Hydrogenase-2 operon protein HybAAdd BLAST301

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP0AAJ8
PRIDEiP0AAJ8

Interactioni

Protein-protein interaction databases

BioGridi4263111, 20 interactors
IntActiP0AAJ8, 6 interactors
STRINGi316385.ECDH10B_3173

Structurei

3D structure databases

ProteinModelPortaliP0AAJ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 684Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST31
Domaini103 – 1344Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST32
Domaini136 – 1654Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105QA4 Bacteria
COG0437 LUCA
HOGENOMiHOG000163383
InParanoidiP0AAJ8
OMAiCEAACNK
PhylomeDBiP0AAJ8

Family and domain databases

InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR019546 TAT_signal_bac_arc
PfamiView protein in Pfam
PF13247 Fer4_11, 1 hit
TIGRFAMsiTIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRNFIKAA SCGALLTGAL PSVSHAAAEN RPPIPGSLGM LYDSTLCVGC
60 70 80 90 100
QACVTKCQDI NFPERNPQGE QTWSNNDKLS PYTNNIIQVW TSGTGVNKDQ
110 120 130 140 150
EENGYAYIKK QCMHCVDPNC VSVCPVSALK KDPKTGIVHY DKDVCTGCRY
160 170 180 190 200
CMVACPYNVP KYDYNNPFGA LHKCELCNQK GVERLDKGGL PGCVEVCPAG
210 220 230 240 250
AVIFGTREEL MAEAKKRLAL KPGSEYHYPR QTLKSGDTYL HTVPKYYPHL
260 270 280 290 300
YGEKEGGGTQ VLVLTGVPYE NLDLPKLDDL STGARSENIQ HTLYKGMMLP
310 320
LAVLAGLTVL VRRNTKNDHH DGGDDHES
Length:328
Mass (Da):36,003
Last modified:October 11, 2005 - v1
Checksum:i77203A0F50F61662
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA Translation: AAA21589.1
U28377 Genomic DNA Translation: AAA69163.1
U00096 Genomic DNA Translation: AAC76032.1
AP009048 Genomic DNA Translation: BAE77057.1
PIRiB65086
RefSeqiNP_417470.1, NC_000913.3
WP_001081870.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76032; AAC76032; b2996
BAE77057; BAE77057; BAE77057
GeneIDi944842
KEGGiecj:JW2964
eco:b2996
PATRICifig|1411691.4.peg.3733

Similar proteinsi

Entry informationi

Entry nameiHYBA_ECOLI
AccessioniPrimary (citable) accession number: P0AAJ8
Secondary accession number(s): P37179, Q2M9J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health