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Protein

Hydrogenase-2 operon protein HybA

Gene

hybA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi50Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi53Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi57Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi112Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi115Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi120Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi124Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi145Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi148Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi151Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi155Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi174Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi177Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi193Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi197Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • anaerobic glycerol catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYBA-MONOMER.
ECOL316407:JW2964-MONOMER.
MetaCyc:HYBA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-2 operon protein HybA
Gene namesi
Name:hybA
Ordered Locus Names:b2996, JW2964
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11799. hybA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Tat-type signalSequence analysisAdd BLAST27
ChainiPRO_000004227328 – 328Hydrogenase-2 operon protein HybAAdd BLAST301

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP0AAJ8.
PRIDEiP0AAJ8.

Interactioni

Protein-protein interaction databases

BioGridi4263111. 7 interactors.
IntActiP0AAJ8. 6 interactors.
STRINGi511145.b2996.

Structurei

3D structure databases

ProteinModelPortaliP0AAJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 684Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST31
Domaini103 – 1344Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST32
Domaini136 – 1654Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Contains 3 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105QA4. Bacteria.
COG0437. LUCA.
HOGENOMiHOG000163383.
InParanoidiP0AAJ8.
OMAiKYDYDNP.
PhylomeDBiP0AAJ8.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRNFIKAA SCGALLTGAL PSVSHAAAEN RPPIPGSLGM LYDSTLCVGC
60 70 80 90 100
QACVTKCQDI NFPERNPQGE QTWSNNDKLS PYTNNIIQVW TSGTGVNKDQ
110 120 130 140 150
EENGYAYIKK QCMHCVDPNC VSVCPVSALK KDPKTGIVHY DKDVCTGCRY
160 170 180 190 200
CMVACPYNVP KYDYNNPFGA LHKCELCNQK GVERLDKGGL PGCVEVCPAG
210 220 230 240 250
AVIFGTREEL MAEAKKRLAL KPGSEYHYPR QTLKSGDTYL HTVPKYYPHL
260 270 280 290 300
YGEKEGGGTQ VLVLTGVPYE NLDLPKLDDL STGARSENIQ HTLYKGMMLP
310 320
LAVLAGLTVL VRRNTKNDHH DGGDDHES
Length:328
Mass (Da):36,003
Last modified:October 11, 2005 - v1
Checksum:i77203A0F50F61662
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA. Translation: AAA21589.1.
U28377 Genomic DNA. Translation: AAA69163.1.
U00096 Genomic DNA. Translation: AAC76032.1.
AP009048 Genomic DNA. Translation: BAE77057.1.
PIRiB65086.
RefSeqiNP_417470.1. NC_000913.3.
WP_001081870.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76032; AAC76032; b2996.
BAE77057; BAE77057; BAE77057.
GeneIDi944842.
KEGGiecj:JW2964.
eco:b2996.
PATRICi32121408. VBIEscCol129921_3091.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA. Translation: AAA21589.1.
U28377 Genomic DNA. Translation: AAA69163.1.
U00096 Genomic DNA. Translation: AAC76032.1.
AP009048 Genomic DNA. Translation: BAE77057.1.
PIRiB65086.
RefSeqiNP_417470.1. NC_000913.3.
WP_001081870.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263111. 7 interactors.
IntActiP0AAJ8. 6 interactors.
STRINGi511145.b2996.

Proteomic databases

PaxDbiP0AAJ8.
PRIDEiP0AAJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76032; AAC76032; b2996.
BAE77057; BAE77057; BAE77057.
GeneIDi944842.
KEGGiecj:JW2964.
eco:b2996.
PATRICi32121408. VBIEscCol129921_3091.

Organism-specific databases

EchoBASEiEB1747.
EcoGeneiEG11799. hybA.

Phylogenomic databases

eggNOGiENOG4105QA4. Bacteria.
COG0437. LUCA.
HOGENOMiHOG000163383.
InParanoidiP0AAJ8.
OMAiKYDYDNP.
PhylomeDBiP0AAJ8.

Enzyme and pathway databases

BioCyciEcoCyc:HYBA-MONOMER.
ECOL316407:JW2964-MONOMER.
MetaCyc:HYBA-MONOMER.

Miscellaneous databases

PROiP0AAJ8.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYBA_ECOLI
AccessioniPrimary (citable) accession number: P0AAJ8
Secondary accession number(s): P37179, Q2M9J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be the small subunit of hydrogenase 2.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.