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P0AAJ8 (HYBA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydrogenase-2 operon protein HybA
Gene names
Name:hybA
Ordered Locus Names:b2996, JW2964
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover.

Cofactor

Binds 4 4Fe-4S clusters By similarity.

Subcellular location

Periplasm.

Post-translational modification

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.

Sequence similarities

Contains 3 4Fe-4S ferredoxin-type domains.

Caution

Was originally (Ref.1) thought to be the small subunit of hydrogenase 2.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainRepeat
Signal
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

electron carrier activity

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

oxidoreductase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727Tat-type signal Potential
Chain28 – 328301Hydrogenase-2 operon protein HybA
PRO_0000042273

Regions

Domain38 – 68314Fe-4S ferredoxin-type 1
Domain103 – 134324Fe-4S ferredoxin-type 2
Domain136 – 165304Fe-4S ferredoxin-type 3

Sites

Metal binding471Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding501Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding531Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding571Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1121Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1151Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1201Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1241Iron-sulfur 4 (4Fe-4S) By similarity
Metal binding1451Iron-sulfur 4 (4Fe-4S) By similarity
Metal binding1481Iron-sulfur 4 (4Fe-4S) By similarity
Metal binding1511Iron-sulfur 4 (4Fe-4S) By similarity
Metal binding1551Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1741Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1771Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1931Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1971Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P0AAJ8 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 77203A0F50F61662

FASTA32836,003
        10         20         30         40         50         60 
MNRRNFIKAA SCGALLTGAL PSVSHAAAEN RPPIPGSLGM LYDSTLCVGC QACVTKCQDI 

        70         80         90        100        110        120 
NFPERNPQGE QTWSNNDKLS PYTNNIIQVW TSGTGVNKDQ EENGYAYIKK QCMHCVDPNC 

       130        140        150        160        170        180 
VSVCPVSALK KDPKTGIVHY DKDVCTGCRY CMVACPYNVP KYDYNNPFGA LHKCELCNQK 

       190        200        210        220        230        240 
GVERLDKGGL PGCVEVCPAG AVIFGTREEL MAEAKKRLAL KPGSEYHYPR QTLKSGDTYL 

       250        260        270        280        290        300 
HTVPKYYPHL YGEKEGGGTQ VLVLTGVPYE NLDLPKLDDL STGARSENIQ HTLYKGMMLP 

       310        320 
LAVLAGLTVL VRRNTKNDHH DGGDDHES

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2."
Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T., Peck H.D. Jr., Przybyla A.E.
J. Bacteriol. 176:4416-4423(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / TG1.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: EXPORT VIA THE TAT-SYSTEM AND THE SEC-SYSTEM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U09177 Genomic DNA. Translation: AAA21589.1.
U28377 Genomic DNA. Translation: AAA69163.1.
U00096 Genomic DNA. Translation: AAC76032.1.
AP009048 Genomic DNA. Translation: BAE77057.1.
PIRB65086.
RefSeqNP_417470.1. NC_000913.2.
YP_491193.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AAJ8.
SMRP0AAJ8. Positions 100-197.
ModBaseSearch...

Protein-protein interaction databases

IntActP0AAJ8. 4 interactions.
STRING511145.b2996.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76032; AAC76032; b2996.
BAE77057; BAE77057; BAE77057.
GeneID12934117.
944842.
KEGGecj:Y75_p2925.
eco:b2996.
PATRIC32121408. VBIEscCol129921_3091.

Organism-specific databases

EchoBASEEB1747.
EcoGeneEG11799. hybA.

Phylogenomic databases

eggNOGCOG0437.
HOGENOMHOG000163383.
OMAGCRYCQI.
ProtClustDBPRK10882.

Enzyme and pathway databases

BioCycEcoCyc:HYBA-MONOMER.
ECOL316407:JW2964-MONOMER.
MetaCyc:HYBA-MONOMER.

Gene expression databases

GenevestigatorP0AAJ8.

Family and domain databases

InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF00037. Fer4. 1 hit.
PF12798. Fer4_3. 1 hit.
PF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 3 hits.
PS51318. TAT. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHYBA_ECOLI
AccessionPrimary (citable) accession number: P0AAJ8
Secondary accession number(s): P37179, Q2M9J9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

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