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Protein

Hydrogenase-2 operon protein HybA

Gene

hybA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi50 – 501Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi53 – 531Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi57 – 571Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi112 – 1121Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi115 – 1151Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi120 – 1201Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi124 – 1241Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi145 – 1451Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi148 – 1481Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi151 – 1511Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi155 – 1551Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi174 – 1741Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi177 – 1771Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi193 – 1931Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi197 – 1971Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

  • anaerobic glycerol catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYBA-MONOMER.
ECOL316407:JW2964-MONOMER.
MetaCyc:HYBA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-2 operon protein HybA
Gene namesi
Name:hybA
Ordered Locus Names:b2996, JW2964
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11799. hybA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Tat-type signalSequence analysisAdd
BLAST
Chaini28 – 328301Hydrogenase-2 operon protein HybAPRO_0000042273Add
BLAST

Post-translational modificationi

Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.

Proteomic databases

PaxDbiP0AAJ8.

Interactioni

Protein-protein interaction databases

BioGridi4263111. 7 interactions.
IntActiP0AAJ8. 6 interactions.
STRINGi511145.b2996.

Structurei

3D structure databases

ProteinModelPortaliP0AAJ8.
SMRiP0AAJ8. Positions 36-62, 100-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 68314Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini103 – 134324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini136 – 165304Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105QA4. Bacteria.
COG0437. LUCA.
HOGENOMiHOG000163383.
InParanoidiP0AAJ8.
OMAiKYDYDNP.
OrthoDBiEOG6XSZQF.
PhylomeDBiP0AAJ8.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRRNFIKAA SCGALLTGAL PSVSHAAAEN RPPIPGSLGM LYDSTLCVGC
60 70 80 90 100
QACVTKCQDI NFPERNPQGE QTWSNNDKLS PYTNNIIQVW TSGTGVNKDQ
110 120 130 140 150
EENGYAYIKK QCMHCVDPNC VSVCPVSALK KDPKTGIVHY DKDVCTGCRY
160 170 180 190 200
CMVACPYNVP KYDYNNPFGA LHKCELCNQK GVERLDKGGL PGCVEVCPAG
210 220 230 240 250
AVIFGTREEL MAEAKKRLAL KPGSEYHYPR QTLKSGDTYL HTVPKYYPHL
260 270 280 290 300
YGEKEGGGTQ VLVLTGVPYE NLDLPKLDDL STGARSENIQ HTLYKGMMLP
310 320
LAVLAGLTVL VRRNTKNDHH DGGDDHES
Length:328
Mass (Da):36,003
Last modified:October 11, 2005 - v1
Checksum:i77203A0F50F61662
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA. Translation: AAA21589.1.
U28377 Genomic DNA. Translation: AAA69163.1.
U00096 Genomic DNA. Translation: AAC76032.1.
AP009048 Genomic DNA. Translation: BAE77057.1.
PIRiB65086.
RefSeqiNP_417470.1. NC_000913.3.
WP_001081870.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76032; AAC76032; b2996.
BAE77057; BAE77057; BAE77057.
GeneIDi944842.
KEGGiecj:JW2964.
eco:b2996.
PATRICi32121408. VBIEscCol129921_3091.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09177 Genomic DNA. Translation: AAA21589.1.
U28377 Genomic DNA. Translation: AAA69163.1.
U00096 Genomic DNA. Translation: AAC76032.1.
AP009048 Genomic DNA. Translation: BAE77057.1.
PIRiB65086.
RefSeqiNP_417470.1. NC_000913.3.
WP_001081870.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAJ8.
SMRiP0AAJ8. Positions 36-62, 100-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263111. 7 interactions.
IntActiP0AAJ8. 6 interactions.
STRINGi511145.b2996.

Proteomic databases

PaxDbiP0AAJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76032; AAC76032; b2996.
BAE77057; BAE77057; BAE77057.
GeneIDi944842.
KEGGiecj:JW2964.
eco:b2996.
PATRICi32121408. VBIEscCol129921_3091.

Organism-specific databases

EchoBASEiEB1747.
EcoGeneiEG11799. hybA.

Phylogenomic databases

eggNOGiENOG4105QA4. Bacteria.
COG0437. LUCA.
HOGENOMiHOG000163383.
InParanoidiP0AAJ8.
OMAiKYDYDNP.
OrthoDBiEOG6XSZQF.
PhylomeDBiP0AAJ8.

Enzyme and pathway databases

BioCyciEcoCyc:HYBA-MONOMER.
ECOL316407:JW2964-MONOMER.
MetaCyc:HYBA-MONOMER.

Miscellaneous databases

PROiP0AAJ8.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2."
    Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T., Peck H.D. Jr., Przybyla A.E.
    J. Bacteriol. 176:4416-4423(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / TG1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides."
    Tullman-Ercek D., DeLisa M.P., Kawarasaki Y., Iranpour P., Ribnicky B., Palmer T., Georgiou G.
    J. Biol. Chem. 282:8309-8316(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: EXPORT VIA THE TAT-SYSTEM AND THE SEC-SYSTEM.

Entry informationi

Entry nameiHYBA_ECOLI
AccessioniPrimary (citable) accession number: P0AAJ8
Secondary accession number(s): P37179, Q2M9J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: June 8, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be the small subunit of hydrogenase 2.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.