UniProtKB - P0AAJ5 (FDOH_ECOLI)
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Protein
Formate dehydrogenase-O iron-sulfur subunit
Gene
fdoH
Organism
Escherichia coli (strain K12)
Status
Functioni
Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit (By similarity).By similarity
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 39 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 42 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 45 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 49 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 100 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 103 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 108 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 112 | Iron-sulfur 4 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 133 | Iron-sulfur 4 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 136 | Iron-sulfur 4 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 139 | Iron-sulfur 4 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 143 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 160 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 163 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 175 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 179 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- formate dehydrogenase (quinone) activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cellular respiration Source: EcoCyc
- formate oxidation Source: EcoCyc
Keywordsi
Biological process | Electron transport, Transport |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:FDOH-MONOMER. MetaCyc:FDOH-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Formate dehydrogenase-O iron-sulfur subunitAlternative name(s): Aerobic formate dehydrogenase iron-sulfur subunit FDH-Z subunit beta Formate dehydrogenase-O subunit beta |
Gene namesi | Name:fdoH Ordered Locus Names:b3893, JW3864 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG11857. fdoH. |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 260 | Cytoplasmic1 PublicationAdd BLAST | 260 | |
Transmembranei | 261 – 279 | HelicalCuratedAdd BLAST | 19 | |
Topological domaini | 280 – 300 | Periplasmic1 PublicationAdd BLAST | 21 |
GO - Cellular componenti
- formate dehydrogenase complex Source: EcoCyc
- integral component of membrane Source: UniProtKB-KW
- intrinsic component of the cytoplasmic side of the plasma membrane Source: EcoCyc
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159249 | 1 – 300 | Formate dehydrogenase-O iron-sulfur subunitAdd BLAST | 300 |
Proteomic databases
PaxDbi | P0AAJ5. |
PRIDEi | P0AAJ5. |
Interactioni
Subunit structurei
Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.
Protein-protein interaction databases
BioGridi | 4263325. 24 interactors. |
IntActi | P0AAJ5. 4 interactors. |
STRINGi | 316385.ECDH10B_4083. |
Structurei
3D structure databases
ProteinModelPortali | P0AAJ5. |
SMRi | P0AAJ5. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 60 | 4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST | 31 | |
Domaini | 91 – 123 | 4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST | 33 | |
Domaini | 124 – 153 | 4Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST | 30 | |
Domaini | 158 – 189 | 4Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST | 32 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG4105QA4. Bacteria. COG0437. LUCA. |
HOGENOMi | HOG000163382. |
InParanoidi | P0AAJ5. |
KOi | K00124. |
OMAi | CKACQSA. |
PhylomeDBi | P0AAJ5. |
Family and domain databases
CDDi | cd10558. FDH-N. 1 hit. |
Gene3Di | 1.20.5.480. 1 hit. |
InterProi | View protein in InterPro IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR006470. Formate_DH_bsu_Proteobacteria. IPR038384. Formate_DH_C_sf. IPR014603. Formate_DH_Fe-S_su. IPR015246. Formate_DH_TM. |
Pfami | View protein in Pfam PF13247. Fer4_11. 1 hit. PF09163. Form-deh_trans. 1 hit. |
PIRSFi | PIRSF036298. FDH_4Fe4S. 1 hit. |
TIGRFAMsi | TIGR01582. FDH-beta. 1 hit. |
PROSITEi | View protein in PROSITE PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 4 hits. |
i Sequence
Sequence statusi: Complete.
P0AAJ5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAYQSQDIIR RSATNGLTPA PQARDFQEEV AKLIDVTTCI GCKACQVACS
60 70 80 90 100
EWNDIRDTVG NNIGVYDNPN DLSAKSWTVM RFSEVEQNDK LEWLIRKDGC
110 120 130 140 150
MHCSDPGCLK ACPAEGAIIQ YANGIVDFQS EQCIGCGYCI AGCPFDIPRL
160 170 180 190 200
NPEDNRVYKC TLCVDRVVVG QEPACVKTCP TGAIHFGTKE SMKTLASERV
210 220 230 240 250
AELKTRGYDN AGLYDPAGVG GTHVMYVLHH ADKPNLYHGL PENPEISETV
260 270 280 290 300
KFWKGIWKPL AAVGFAATFA ASIFHYVGVG PNRADEEENN LHEEKDEERK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19201 Genomic DNA. Translation: AAB03026.1. U00096 Genomic DNA. Translation: AAD13455.1. AP009048 Genomic DNA. Translation: BAE77416.1. |
PIRi | S40837. |
RefSeqi | NP_418329.1. NC_000913.3. WP_000331377.1. NZ_LN832404.1. |
Genome annotation databases
EnsemblBacteriai | AAD13455; AAD13455; b3893. BAE77416; BAE77416; BAE77416. |
GeneIDi | 948395. |
KEGGi | ecj:JW3864. eco:b3893. |
PATRICi | fig|1411691.4.peg.2816. |
Similar proteinsi
Entry informationi
Entry namei | FDOH_ECOLI | |
Accessioni | P0AAJ5Primary (citable) accession number: P0AAJ5 Secondary accession number(s): P32175, Q2M8J0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | October 11, 2005 | |
Last modified: | March 28, 2018 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |