fdoH

Functionⁱ

Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit (By similarity).By similarity

Cofactorⁱ

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	39	Iron-sulfur 1 (4Fe-4S)By similarity	1
Metal bindingⁱ	42	Iron-sulfur 1 (4Fe-4S)By similarity	1
Metal bindingⁱ	45	Iron-sulfur 1 (4Fe-4S)By similarity	1
Metal bindingⁱ	49	Iron-sulfur 2 (4Fe-4S)By similarity	1
Metal bindingⁱ	100	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	103	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	108	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	112	Iron-sulfur 4 (4Fe-4S)By similarity	1
Metal bindingⁱ	133	Iron-sulfur 4 (4Fe-4S)By similarity	1
Metal bindingⁱ	136	Iron-sulfur 4 (4Fe-4S)By similarity	1
Metal bindingⁱ	139	Iron-sulfur 4 (4Fe-4S)By similarity	1
Metal bindingⁱ	143	Iron-sulfur 3 (4Fe-4S)By similarity	1
Metal bindingⁱ	160	Iron-sulfur 2 (4Fe-4S)By similarity	1
Metal bindingⁱ	163	Iron-sulfur 2 (4Fe-4S)By similarity	1
Metal bindingⁱ	175	Iron-sulfur 2 (4Fe-4S)By similarity	1
Metal bindingⁱ	179	Iron-sulfur 1 (4Fe-4S)By similarity	1

GO - Molecular functionⁱ

4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
formate dehydrogenase (quinone) activity
Source: EcoCyc
Inferred from direct assayⁱ
- 1504073
metal ion binding Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

Complete GO annotation...

Keywordsⁱ

Biological process	Electron transport, Transport
Ligand	4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:FDOH-MONOMER. MetaCyc:FDOH-MONOMER.

BioCycⁱ

EcoCyc:FDOH-MONOMER.
MetaCyc:FDOH-MONOMER.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Formate dehydrogenase-O iron-sulfur subunit Alternative name(s): Aerobic formate dehydrogenase iron-sulfur subunit FDH-Z subunit beta Formate dehydrogenase-O subunit beta
Gene namesⁱ	Name:fdoH Ordered Locus Names:b3893, JW3864
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11857. fdoH.

EcoGeneⁱ

EG11857. fdoH.

Subcellular locationⁱ

Cell membrane; Single-pass membrane protein

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 260	Cytoplasmic1 PublicationAdd BLAST	260
Transmembraneⁱ	261 – 279	HelicalCuratedAdd BLAST	19
Topological domainⁱ	280 – 300	Periplasmic1 PublicationAdd BLAST	21

GO - Cellular componentⁱ

formate dehydrogenase complex
Source: EcoCyc
Inferred from direct assayⁱ
- 1504073
- 8522521
integral component of membrane Source: UniProtKB-KW
intrinsic component of the cytoplasmic side of the plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 9852007
membrane
Source: UniProtKB
Inferred from direct assayⁱ
- 16858726

Complete GO annotation...

Keywords - Cellular componentⁱ

Cell membrane, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000159249	1 – 300	Formate dehydrogenase-O iron-sulfur subunitAdd BLAST		300

Proteomic databases

PaxDbⁱ	P0AAJ5.
PRIDEⁱ	P0AAJ5.

Interactionⁱ

Subunit structureⁱ

Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.

Protein-protein interaction databases

BioGridⁱ	4263325. 18 interactors.
IntActⁱ	P0AAJ5. 4 interactors.
STRINGⁱ	511145.b3893.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0AAJ5.
SMRⁱ	P0AAJ5.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	30 – 60	4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST	31
Domainⁱ	91 – 123	4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST	33
Domainⁱ	124 – 153	4Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST	30
Domainⁱ	158 – 189	4Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST	32

Keywords - Domainⁱ

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4105QA4. Bacteria. COG0437. LUCA.
HOGENOMⁱ	HOG000163382.
InParanoidⁱ	P0AAJ5.
KEGG Orthology (KO) More...KOⁱ	K00124.
PhylomeDBⁱ	P0AAJ5.

Family and domain databases

InterProⁱ	View protein in InterPro IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR006470. Formate_DH_bsu_Proteobacteria. IPR014603. Formate_DH_Fe-S_su. IPR015246. Formate_DH_TM.
PANTHERⁱ	PTHR43545:SF3. PTHR43545:SF3. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13247. Fer4_11. 1 hit. PF09163. Form-deh_trans. 1 hit.
PIRSFⁱ	PIRSF036298. FDH_4Fe4S. 1 hit.
TIGRFAMsⁱ	TIGR01582. FDH-beta. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 4 hits.

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AAJ5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAYQSQDIIR RSATNGLTPA PQARDFQEEV AKLIDVTTCI GCKACQVACS 
        60         70         80         90        100
EWNDIRDTVG NNIGVYDNPN DLSAKSWTVM RFSEVEQNDK LEWLIRKDGC 
       110        120        130        140        150
MHCSDPGCLK ACPAEGAIIQ YANGIVDFQS EQCIGCGYCI AGCPFDIPRL 
       160        170        180        190        200
NPEDNRVYKC TLCVDRVVVG QEPACVKTCP TGAIHFGTKE SMKTLASERV 
       210        220        230        240        250
AELKTRGYDN AGLYDPAGVG GTHVMYVLHH ADKPNLYHGL PENPEISETV 
       260        270        280        290        300
KFWKGIWKPL AAVGFAATFA ASIFHYVGVG PNRADEEENN LHEEKDEERK

Length:300

Mass (Da):33,100

Last modified:October 11, 2005 - v1

Checksum:ⁱ950B40FE1A6016E7

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L19201 Genomic DNA. Translation: AAB03026.1. U00096 Genomic DNA. Translation: AAD13455.1. AP009048 Genomic DNA. Translation: BAE77416.1.
PIRⁱ	S40837.
NCBI Reference Sequences More...RefSeqⁱ	NP_418329.1. NC_000913.3. WP_000331377.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAD13455; AAD13455; b3893. BAE77416; BAE77416; BAE77416.
GeneIDⁱ	948395.
KEGGⁱ	ecj:JW3864. eco:b3893.
PATRICⁱ	fig\|1411691.4.peg.2816.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L19201 Genomic DNA. Translation: AAB03026.1. U00096 Genomic DNA. Translation: AAD13455.1. AP009048 Genomic DNA. Translation: BAE77416.1.
PIRⁱ	S40837.
NCBI Reference Sequences More...RefSeqⁱ	NP_418329.1. NC_000913.3. WP_000331377.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortalⁱ	P0AAJ5.
SMRⁱ	P0AAJ5.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263325. 18 interactors.
IntActⁱ	P0AAJ5. 4 interactors.
STRINGⁱ	511145.b3893.

Proteomic databases

PaxDbⁱ	P0AAJ5.
PRIDEⁱ	P0AAJ5.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAD13455; AAD13455; b3893. BAE77416; BAE77416; BAE77416.
GeneIDⁱ	948395.
KEGGⁱ	ecj:JW3864. eco:b3893.
PATRICⁱ	fig\|1411691.4.peg.2816.

Organism-specific databases

EchoBASEⁱ	EB1803.
EcoGeneⁱ	EG11857. fdoH.

Phylogenomic databases

eggNOGⁱ	ENOG4105QA4. Bacteria. COG0437. LUCA.
HOGENOMⁱ	HOG000163382.
InParanoidⁱ	P0AAJ5.
KEGG Orthology (KO) More...KOⁱ	K00124.
PhylomeDBⁱ	P0AAJ5.

Enzyme and pathway databases

BioCycⁱ	EcoCyc:FDOH-MONOMER. MetaCyc:FDOH-MONOMER.

BioCycⁱ

EcoCyc:FDOH-MONOMER.
MetaCyc:FDOH-MONOMER.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0AAJ5.

PROⁱ

PR:P0AAJ5.

Family and domain databases

InterProⁱ	View protein in InterPro IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR006470. Formate_DH_bsu_Proteobacteria. IPR014603. Formate_DH_Fe-S_su. IPR015246. Formate_DH_TM.
PANTHERⁱ	PTHR43545:SF3. PTHR43545:SF3. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13247. Fer4_11. 1 hit. PF09163. Form-deh_trans. 1 hit.
PIRSFⁱ	PIRSF036298. FDH_4Fe4S. 1 hit.
TIGRFAMsⁱ	TIGR01582. FDH-beta. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 4 hits.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	FDOH_ECOLI
Accessionⁱ	P0AAJ5Primary (citable) accession number: P0AAJ5 Secondary accession number(s): P32175, Q2M8J0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
	Last sequence update:	October 11, 2005
	Last modified:	June 7, 2017
	This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0AAJ5 (FDOH_ECOLI)

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Formate dehydrogenase-O iron-sulfur subunit

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ