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P0AAI9 (FABD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malonyl CoA-acyl carrier protein transacylase

Short name=MCT
EC=2.3.1.39
Gene names
Name:fabD
Synonyms:tfpA
Ordered Locus Names:b1092, JW1078
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].

Pathway

Lipid metabolism; fatty acid biosynthesis.

Sequence similarities

Belongs to the FabD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.8 Ref.9
Chain2 – 309308Malonyl CoA-acyl carrier protein transacylase
PRO_0000194213

Sites

Active site921
Active site2011

Secondary structure

................................................. 309
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AAI9 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 3572E0681D14AB4A

FASTA30932,417
        10         20         30         40         50         60 
MTQFAFVFPG QGSQTVGMLA DMAASYPIVE ETFAEASAAL GYDLWALTQQ GPAEELNKTW 

        70         80         90        100        110        120 
QTQPALLTAS VALYRVWQQQ GGKAPAMMAG HSLGEYSALV CAGVIDFADA VRLVEMRGKF 

       130        140        150        160        170        180 
MQEAVPEGTG AMAAIIGLDD ASIAKACEEA AEGQVVSPVN FNSPGQVVIA GHKEAVERAG 

       190        200        210        220        230        240 
AACKAAGAKR ALPLPVSVPS HCALMKPAAD KLAVELAKIT FNAPTVPVVN NVDVKCETNG 

       250        260        270        280        290        300 
DAIRDALVRQ LYNPVQWTKS VEYMAAQGVE HLYEVGPGKV LTGLTKRIVD TLTASALNEP 


SAMAAALEL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli."
Magnuson K., Oh W., Larson T.J., Cronan J.E. Jr.
FEBS Lett. 299:262-266(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
Strain: K12.
[2]"Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase."
Verwoert I.I.G.S., Verbree E.C., van der Linden K.H., Nijkamp H.J., Stuitje A.R.
J. Bacteriol. 174:2851-2857(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Resistance to trifluoroperazine, a calmodulin inhibitor, maps to the fabD locus in Escherichia coli."
Bouquin N., Tempete M., Holland I.B., Seror S.J.
Mol. Gen. Genet. 246:628-637(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[6]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]"The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes."
Rawlings M., Cronan J.E. Jr.
J. Biol. Chem. 267:5751-5754(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 289-309.
Strain: K12.
[8]Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F.
Submitted (SEP-1994) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-12.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[9]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11.
Strain: K12 / EMG2.
[10]"The Escherichia coli malonyl-CoA:acyl carrier protein transacylase at 1.5-A resolution. Crystal structure of a fatty acid synthase component."
Serre L., Verbree E.C., Dauter Z., Stuitje A.R., Derewenda Z.S.
J. Biol. Chem. 270:12961-12964(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M87040 Genomic DNA. Translation: AAA23742.1.
Z11565 Genomic DNA. Translation: CAA77658.1.
U00096 Genomic DNA. Translation: AAC74176.1.
AP009048 Genomic DNA. Translation: BAA35900.1.
M84991 Genomic DNA. Translation: AAA23738.1.
PIRB41856.
RefSeqNP_415610.1. NC_000913.3.
YP_489360.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MLAX-ray1.50A1-309[»]
2G1HX-ray1.86A2-308[»]
2G2OX-ray1.76A2-308[»]
2G2YX-ray2.26A2-308[»]
2G2ZX-ray2.80A2-308[»]
ProteinModelPortalP0AAI9.
SMRP0AAI9. Positions 3-307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47923N.
IntActP0AAI9. 3 interactions.
STRING511145.b1092.

2D gel databases

SWISS-2DPAGEP0AAI9.

Proteomic databases

PaxDbP0AAI9.
PRIDEP0AAI9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74176; AAC74176; b1092.
BAA35900; BAA35900; BAA35900.
GeneID12934374.
945766.
KEGGecj:Y75_p1062.
eco:b1092.
PATRIC32117425. VBIEscCol129921_1135.

Organism-specific databases

EchoBASEEB1293.
EcoGeneEG11317. fabD.

Phylogenomic databases

eggNOGCOG0331.
HOGENOMHOG000036503.
KOK00645.
OMAPVVTNVE.
OrthoDBEOG6W19KW.
PhylomeDBP0AAI9.
ProtClustDBCLSK879951.

Enzyme and pathway databases

BioCycEcoCyc:MALONYL-COA-ACP-TRANSACYL-MONOMER.
ECOL316407:JW1078-MONOMER.
MetaCyc:MALONYL-COA-ACP-TRANSACYL-MONOMER.
UniPathwayUPA00094.

Gene expression databases

GenevestigatorP0AAI9.

Family and domain databases

Gene3D3.40.366.10. 2 hits.
InterProIPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR024925. Malonyl_CoA-ACP_transAc.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view]
PfamPF00698. Acyl_transf_1. 1 hit.
[Graphical view]
PIRSFPIRSF000446. Mct. 1 hit.
SUPFAMSSF52151. SSF52151. 2 hits.
SSF55048. SSF55048. 1 hit.
TIGRFAMsTIGR00128. fabD. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0AAI9.
PROP0AAI9.

Entry information

Entry nameFABD_ECOLI
AccessionPrimary (citable) accession number: P0AAI9
Secondary accession number(s): P25715
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene