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P0AAI9

- FABD_ECOLI

UniProt

P0AAI9 - FABD_ECOLI

Protein

Malonyl CoA-acyl carrier protein transacylase

Gene

fabD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921
    Active sitei201 – 2011

    GO - Molecular functioni

    1. [acyl-carrier-protein] S-malonyltransferase activity Source: EcoCyc

    GO - Biological processi

    1. fatty acid biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciEcoCyc:MALONYL-COA-ACP-TRANSACYL-MONOMER.
    ECOL316407:JW1078-MONOMER.
    MetaCyc:MALONYL-COA-ACP-TRANSACYL-MONOMER.
    RETL1328306-WGS:GSTH-1455-MONOMER.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malonyl CoA-acyl carrier protein transacylase (EC:2.3.1.39)
    Short name:
    MCT
    Gene namesi
    Name:fabD
    Synonyms:tfpA
    Ordered Locus Names:b1092, JW1078
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11317. fabD.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 309308Malonyl CoA-acyl carrier protein transacylasePRO_0000194213Add
    BLAST

    Proteomic databases

    PaxDbiP0AAI9.
    PRIDEiP0AAI9.

    2D gel databases

    SWISS-2DPAGEP0AAI9.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AAI9.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-47923N.
    IntActiP0AAI9. 4 interactions.
    STRINGi511145.b1092.

    Structurei

    Secondary structure

    1
    309
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 85
    Turni16 – 194
    Helixi20 – 256
    Helixi28 – 4013
    Helixi44 – 507
    Helixi53 – 564
    Helixi59 – 7921
    Beta strandi86 – 916
    Helixi93 – 1019
    Helixi107 – 12418
    Beta strandi129 – 1379
    Helixi140 – 15011
    Beta strandi156 – 1638
    Beta strandi166 – 1727
    Helixi173 – 18513
    Beta strandi189 – 1935
    Helixi203 – 2053
    Helixi206 – 21712
    Turni231 – 2333
    Helixi240 – 25213
    Helixi257 – 26610
    Beta strandi271 – 2744
    Beta strandi276 – 2794
    Helixi280 – 2889
    Beta strandi293 – 2964
    Helixi300 – 3067

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MLAX-ray1.50A1-309[»]
    2G1HX-ray1.86A2-309[»]
    2G2OX-ray1.76A2-309[»]
    2G2YX-ray2.26A2-309[»]
    2G2ZX-ray2.80A2-309[»]
    ProteinModelPortaliP0AAI9.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AAI9.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FabD family.Curated

    Phylogenomic databases

    eggNOGiCOG0331.
    HOGENOMiHOG000036503.
    KOiK00645.
    OMAiIWRVWQE.
    OrthoDBiEOG6W19KW.
    PhylomeDBiP0AAI9.

    Family and domain databases

    Gene3Di3.40.366.10. 2 hits.
    InterProiIPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR024925. Malonyl_CoA-ACP_transAc.
    IPR004410. Malonyl_CoA-ACP_transAc_FabD.
    IPR016036. Malonyl_transacylase_ACP-bd.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000446. Mct. 1 hit.
    SUPFAMiSSF52151. SSF52151. 2 hits.
    SSF55048. SSF55048. 1 hit.
    TIGRFAMsiTIGR00128. fabD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AAI9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQFAFVFPG QGSQTVGMLA DMAASYPIVE ETFAEASAAL GYDLWALTQQ    50
    GPAEELNKTW QTQPALLTAS VALYRVWQQQ GGKAPAMMAG HSLGEYSALV 100
    CAGVIDFADA VRLVEMRGKF MQEAVPEGTG AMAAIIGLDD ASIAKACEEA 150
    AEGQVVSPVN FNSPGQVVIA GHKEAVERAG AACKAAGAKR ALPLPVSVPS 200
    HCALMKPAAD KLAVELAKIT FNAPTVPVVN NVDVKCETNG DAIRDALVRQ 250
    LYNPVQWTKS VEYMAAQGVE HLYEVGPGKV LTGLTKRIVD TLTASALNEP 300
    SAMAAALEL 309
    Length:309
    Mass (Da):32,417
    Last modified:January 23, 2007 - v2
    Checksum:i3572E0681D14AB4A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87040 Genomic DNA. Translation: AAA23742.1.
    Z11565 Genomic DNA. Translation: CAA77658.1.
    U00096 Genomic DNA. Translation: AAC74176.1.
    AP009048 Genomic DNA. Translation: BAA35900.1.
    M84991 Genomic DNA. Translation: AAA23738.1.
    PIRiB41856.
    RefSeqiNP_415610.1. NC_000913.3.
    YP_489360.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74176; AAC74176; b1092.
    BAA35900; BAA35900; BAA35900.
    GeneIDi12934374.
    945766.
    KEGGiecj:Y75_p1062.
    eco:b1092.
    PATRICi32117425. VBIEscCol129921_1135.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87040 Genomic DNA. Translation: AAA23742.1 .
    Z11565 Genomic DNA. Translation: CAA77658.1 .
    U00096 Genomic DNA. Translation: AAC74176.1 .
    AP009048 Genomic DNA. Translation: BAA35900.1 .
    M84991 Genomic DNA. Translation: AAA23738.1 .
    PIRi B41856.
    RefSeqi NP_415610.1. NC_000913.3.
    YP_489360.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MLA X-ray 1.50 A 1-309 [» ]
    2G1H X-ray 1.86 A 2-309 [» ]
    2G2O X-ray 1.76 A 2-309 [» ]
    2G2Y X-ray 2.26 A 2-309 [» ]
    2G2Z X-ray 2.80 A 2-309 [» ]
    ProteinModelPortali P0AAI9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47923N.
    IntActi P0AAI9. 4 interactions.
    STRINGi 511145.b1092.

    2D gel databases

    SWISS-2DPAGE P0AAI9.

    Proteomic databases

    PaxDbi P0AAI9.
    PRIDEi P0AAI9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74176 ; AAC74176 ; b1092 .
    BAA35900 ; BAA35900 ; BAA35900 .
    GeneIDi 12934374.
    945766.
    KEGGi ecj:Y75_p1062.
    eco:b1092.
    PATRICi 32117425. VBIEscCol129921_1135.

    Organism-specific databases

    EchoBASEi EB1293.
    EcoGenei EG11317. fabD.

    Phylogenomic databases

    eggNOGi COG0331.
    HOGENOMi HOG000036503.
    KOi K00645.
    OMAi IWRVWQE.
    OrthoDBi EOG6W19KW.
    PhylomeDBi P0AAI9.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci EcoCyc:MALONYL-COA-ACP-TRANSACYL-MONOMER.
    ECOL316407:JW1078-MONOMER.
    MetaCyc:MALONYL-COA-ACP-TRANSACYL-MONOMER.
    RETL1328306-WGS:GSTH-1455-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AAI9.
    PROi P0AAI9.

    Gene expression databases

    Genevestigatori P0AAI9.

    Family and domain databases

    Gene3Di 3.40.366.10. 2 hits.
    InterProi IPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR024925. Malonyl_CoA-ACP_transAc.
    IPR004410. Malonyl_CoA-ACP_transAc_FabD.
    IPR016036. Malonyl_transacylase_ACP-bd.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000446. Mct. 1 hit.
    SUPFAMi SSF52151. SSF52151. 2 hits.
    SSF55048. SSF55048. 1 hit.
    TIGRFAMsi TIGR00128. fabD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli."
      Magnuson K., Oh W., Larson T.J., Cronan J.E. Jr.
      FEBS Lett. 299:262-266(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
      Strain: K12.
    2. "Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase."
      Verwoert I.I.G.S., Verbree E.C., van der Linden K.H., Nijkamp H.J., Stuitje A.R.
      J. Bacteriol. 174:2851-2857(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Resistance to trifluoroperazine, a calmodulin inhibitor, maps to the fabD locus in Escherichia coli."
      Bouquin N., Tempete M., Holland I.B., Seror S.J.
      Mol. Gen. Genet. 246:628-637(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    7. "The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes."
      Rawlings M., Cronan J.E. Jr.
      J. Biol. Chem. 267:5751-5754(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 289-309.
      Strain: K12.
    8. Cited for: PROTEIN SEQUENCE OF 2-12.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    9. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11.
      Strain: K12 / EMG2.
    10. "The Escherichia coli malonyl-CoA:acyl carrier protein transacylase at 1.5-A resolution. Crystal structure of a fatty acid synthase component."
      Serre L., Verbree E.C., Dauter Z., Stuitje A.R., Derewenda Z.S.
      J. Biol. Chem. 270:12961-12964(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

    Entry informationi

    Entry nameiFABD_ECOLI
    AccessioniPrimary (citable) accession number: P0AAI9
    Secondary accession number(s): P25715
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3