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Protein

3-oxoacyl-[acyl-carrier-protein] synthase 2

Gene

fabF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis.2 Publications

Catalytic activityi

(Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].1 Publication

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1641 Publication1

GO - Molecular functioni

  • 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: EcoCyc
  • beta-ketoacyl-acyl-carrier-protein synthase II activity Source: CACAO

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:3-OXOACYL-ACP-SYNTHII-MONOMER
MetaCyc:3-OXOACYL-ACP-SYNTHII-MONOMER
BRENDAi2.3.1.179 2026
SABIO-RKiP0AAI5
UniPathwayiUPA00094

Chemistry databases

SwissLipidsiSLP:000001811

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 2 (EC:2.3.1.179)
Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase II
Beta-ketoacyl-ACP synthase II
Short name:
KAS II
Gene namesi
Name:fabF
Synonyms:fabJ
Ordered Locus Names:b1095, JW1081
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12606 fabF

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB

Pathology & Biotechi

Chemistry databases

DrugBankiDB01034 Cerulenin
DB09414 Dipotassium phosphate
DB03017 Lauric Acid
DB08407 PLATENSIMYCIN

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001803142 – 4133-oxoacyl-[acyl-carrier-protein] synthase 2Add BLAST412

Proteomic databases

EPDiP0AAI5
PaxDbiP0AAI5
PRIDEiP0AAI5

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaKP0A6Y83EBI-542783,EBI-542092

Protein-protein interaction databases

BioGridi4260072, 208 interactors
DIPiDIP-29377N
IntActiP0AAI5, 6 interactors
STRINGi316385.ECDH10B_1167

Structurei

Secondary structure

1413
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 15Combined sources10
Beta strandi18 – 20Combined sources3
Helixi21 – 29Combined sources9
Beta strandi35 – 37Combined sources3
Beta strandi50 – 52Combined sources3
Turni60 – 62Combined sources3
Helixi65 – 68Combined sources4
Helixi73 – 89Combined sources17
Turni95 – 97Combined sources3
Helixi98 – 100Combined sources3
Beta strandi101 – 106Combined sources6
Helixi112 – 125Combined sources14
Helixi127 – 129Combined sources3
Helixi134 – 138Combined sources5
Helixi142 – 151Combined sources10
Helixi163 – 165Combined sources3
Helixi166 – 180Combined sources15
Beta strandi184 – 192Combined sources9
Helixi197 – 205Combined sources9
Helixi216 – 219Combined sources4
Beta strandi235 – 243Combined sources9
Helixi244 – 249Combined sources6
Beta strandi256 – 265Combined sources10
Beta strandi270 – 272Combined sources3
Helixi278 – 291Combined sources14
Helixi295 – 297Combined sources3
Beta strandi300 – 302Combined sources3
Helixi309 – 323Combined sources15
Helixi324 – 328Combined sources5
Beta strandi330 – 333Combined sources4
Helixi336 – 339Combined sources4
Helixi343 – 345Combined sources3
Helixi346 – 360Combined sources15
Beta strandi370 – 372Combined sources3
Beta strandi382 – 384Combined sources3
Beta strandi393 – 400Combined sources8
Turni401 – 403Combined sources3
Beta strandi404 – 411Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B3NX-ray2.65A2-413[»]
1KASX-ray2.40A2-413[»]
2GFVX-ray2.29A2-413[»]
2GFWX-ray2.40A2-413[»]
2GFXX-ray2.59A2-413[»]
2GFYX-ray2.85A2-413[»]
3G0YX-ray2.60A2-413[»]
3G11X-ray2.00A2-413[»]
3HNZX-ray2.75A2-413[»]
3HO2X-ray2.00A2-413[»]
3HO9X-ray1.90A2-413[»]
3I8PX-ray1.90A2-413[»]
ProteinModelPortaliP0AAI5
SMRiP0AAI5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AAI5

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-ketoacyl-ACP synthases family.Curated

Phylogenomic databases

eggNOGiENOG4105C0Q Bacteria
COG0304 LUCA
HOGENOMiHOG000060165
InParanoidiP0AAI5
KOiK09458
OMAiPFFVPKI
PhylomeDBiP0AAI5

Family and domain databases

Gene3Di3.40.47.10, 2 hits
InterProiView protein in InterPro
IPR017568 3-oxoacyl-ACP_synth-2
IPR018201 Ketoacyl_synth_AS
IPR014031 Ketoacyl_synth_C
IPR014030 Ketoacyl_synth_N
IPR020841 PKS_Beta-ketoAc_synthase_dom
IPR016039 Thiolase-like
PfamiView protein in Pfam
PF00109 ketoacyl-synt, 1 hit
PF02801 Ketoacyl-synt_C, 1 hit
PIRSFiPIRSF000447 KAS_II, 1 hit
SMARTiView protein in SMART
SM00825 PKS_KS, 1 hit
SUPFAMiSSF53901 SSF53901, 2 hits
TIGRFAMsiTIGR03150 fabF, 1 hit
PROSITEiView protein in PROSITE
PS00606 B_KETOACYL_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRRVVVTG LGMLSPVGNT VESTWKALLA GQSGISLIDH FDTSAYATKF
60 70 80 90 100
AGLVKDFNCE DIISRKEQRK MDAFIQYGIV AGVQAMQDSG LEITEENATR
110 120 130 140 150
IGAAIGSGIG GLGLIEENHT SLMNGGPRKI SPFFVPSTIV NMVAGHLTIM
160 170 180 190 200
YGLRGPSISI ATACTSGVHN IGHAARIIAY GDADVMVAGG AEKASTPLGV
210 220 230 240 250
GGFGAARALS TRNDNPQAAS RPWDKERDGF VLGDGAGMLV LEEYEHAKKR
260 270 280 290 300
GAKIYAELVG FGMSSDAYHM TSPPENGAGA ALAMANALRD AGIEASQIGY
310 320 330 340 350
VNAHGTSTPA GDKAEAQAVK TIFGEAASRV LVSSTKSMTG HLLGAAGAVE
360 370 380 390 400
SIYSILALRD QAVPPTINLD NPDEGCDLDF VPHEARQVSG MEYTLCNSFG
410
FGGTNGSLIF KKI
Length:413
Mass (Da):43,046
Last modified:January 23, 2007 - v2
Checksum:i5F60DB1F986B2EE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34979 Genomic DNA Translation: CAA84431.1
U20767 Genomic DNA Translation: AAA83255.1
U00096 Genomic DNA Translation: AAC74179.1
AP009048 Genomic DNA Translation: BAA35903.1
PIRiI41060
RefSeqiNP_415613.1, NC_000913.3
WP_000044679.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74179; AAC74179; b1095
BAA35903; BAA35903; BAA35903
GeneIDi946665
KEGGiecj:JW1081
eco:b1095
PATRICifig|1411691.4.peg.1173

Similar proteinsi

Entry informationi

Entry nameiFABF_ECOLI
AccessioniPrimary (citable) accession number: P0AAI5
Secondary accession number(s): P39435
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health