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Protein

3-oxoacyl-[acyl-carrier-protein] synthase 2

Gene

fabF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis.2 Publications

Catalytic activityi

(Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].1 Publication

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1641 Publication1

GO - Molecular functioni

  • 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: EcoCyc
  • beta-ketoacyl-acyl-carrier-protein synthase II activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:3-OXOACYL-ACP-SYNTHII-MONOMER.
ECOL316407:JW1081-MONOMER.
MetaCyc:3-OXOACYL-ACP-SYNTHII-MONOMER.
BRENDAi2.3.1.179. 2026.
SABIO-RKP0AAI5.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 2 (EC:2.3.1.179)
Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase II
Beta-ketoacyl-ACP synthase II
Short name:
KAS II
Gene namesi
Name:fabF
Synonyms:fabJ
Ordered Locus Names:b1095, JW1081
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12606. fabF.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

DrugBankiDB01034. Cerulenin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001803142 – 4133-oxoacyl-[acyl-carrier-protein] synthase 2Add BLAST412

Proteomic databases

EPDiP0AAI5.
PaxDbiP0AAI5.
PRIDEiP0AAI5.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaKP0A6Y82EBI-542783,EBI-542092

Protein-protein interaction databases

BioGridi4260072. 201 interactors.
DIPiDIP-29377N.
IntActiP0AAI5. 6 interactors.
MINTiMINT-1231688.
STRINGi511145.b1095.

Structurei

Secondary structure

1413
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 15Combined sources10
Beta strandi18 – 20Combined sources3
Helixi21 – 29Combined sources9
Beta strandi35 – 37Combined sources3
Beta strandi50 – 52Combined sources3
Turni60 – 62Combined sources3
Helixi65 – 68Combined sources4
Helixi73 – 89Combined sources17
Turni95 – 97Combined sources3
Helixi98 – 100Combined sources3
Beta strandi101 – 106Combined sources6
Helixi112 – 125Combined sources14
Helixi127 – 129Combined sources3
Helixi134 – 138Combined sources5
Helixi142 – 151Combined sources10
Helixi163 – 165Combined sources3
Helixi166 – 180Combined sources15
Beta strandi184 – 192Combined sources9
Helixi197 – 205Combined sources9
Helixi216 – 219Combined sources4
Beta strandi235 – 243Combined sources9
Helixi244 – 249Combined sources6
Beta strandi256 – 265Combined sources10
Beta strandi270 – 272Combined sources3
Helixi278 – 291Combined sources14
Helixi295 – 297Combined sources3
Beta strandi300 – 302Combined sources3
Helixi309 – 323Combined sources15
Helixi324 – 328Combined sources5
Beta strandi330 – 333Combined sources4
Helixi336 – 339Combined sources4
Helixi343 – 345Combined sources3
Helixi346 – 360Combined sources15
Beta strandi370 – 372Combined sources3
Beta strandi382 – 384Combined sources3
Beta strandi393 – 400Combined sources8
Turni401 – 403Combined sources3
Beta strandi404 – 411Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B3NX-ray2.65A2-413[»]
1KASX-ray2.40A2-413[»]
2GFVX-ray2.29A2-413[»]
2GFWX-ray2.40A2-413[»]
2GFXX-ray2.59A2-413[»]
2GFYX-ray2.85A2-413[»]
3G0YX-ray2.60A2-413[»]
3G11X-ray2.00A2-413[»]
3HNZX-ray2.75A2-413[»]
3HO2X-ray2.00A2-413[»]
3HO9X-ray1.90A2-413[»]
3I8PX-ray1.90A2-413[»]
ProteinModelPortaliP0AAI5.
SMRiP0AAI5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AAI5.

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-ketoacyl-ACP synthases family.Curated

Phylogenomic databases

eggNOGiENOG4105C0Q. Bacteria.
COG0304. LUCA.
HOGENOMiHOG000060165.
InParanoidiP0AAI5.
KOiK09458.
OMAiSIKHGFM.
PhylomeDBiP0AAI5.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000447. KAS_II. 1 hit.
SMARTiSM00825. PKS_KS. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRRVVVTG LGMLSPVGNT VESTWKALLA GQSGISLIDH FDTSAYATKF
60 70 80 90 100
AGLVKDFNCE DIISRKEQRK MDAFIQYGIV AGVQAMQDSG LEITEENATR
110 120 130 140 150
IGAAIGSGIG GLGLIEENHT SLMNGGPRKI SPFFVPSTIV NMVAGHLTIM
160 170 180 190 200
YGLRGPSISI ATACTSGVHN IGHAARIIAY GDADVMVAGG AEKASTPLGV
210 220 230 240 250
GGFGAARALS TRNDNPQAAS RPWDKERDGF VLGDGAGMLV LEEYEHAKKR
260 270 280 290 300
GAKIYAELVG FGMSSDAYHM TSPPENGAGA ALAMANALRD AGIEASQIGY
310 320 330 340 350
VNAHGTSTPA GDKAEAQAVK TIFGEAASRV LVSSTKSMTG HLLGAAGAVE
360 370 380 390 400
SIYSILALRD QAVPPTINLD NPDEGCDLDF VPHEARQVSG MEYTLCNSFG
410
FGGTNGSLIF KKI
Length:413
Mass (Da):43,046
Last modified:January 23, 2007 - v2
Checksum:i5F60DB1F986B2EE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34979 Genomic DNA. Translation: CAA84431.1.
U20767 Genomic DNA. Translation: AAA83255.1.
U00096 Genomic DNA. Translation: AAC74179.1.
AP009048 Genomic DNA. Translation: BAA35903.1.
PIRiI41060.
RefSeqiNP_415613.1. NC_000913.3.
WP_000044679.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74179; AAC74179; b1095.
BAA35903; BAA35903; BAA35903.
GeneIDi946665.
KEGGiecj:JW1081.
eco:b1095.
PATRICi32117431. VBIEscCol129921_1138.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34979 Genomic DNA. Translation: CAA84431.1.
U20767 Genomic DNA. Translation: AAA83255.1.
U00096 Genomic DNA. Translation: AAC74179.1.
AP009048 Genomic DNA. Translation: BAA35903.1.
PIRiI41060.
RefSeqiNP_415613.1. NC_000913.3.
WP_000044679.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B3NX-ray2.65A2-413[»]
1KASX-ray2.40A2-413[»]
2GFVX-ray2.29A2-413[»]
2GFWX-ray2.40A2-413[»]
2GFXX-ray2.59A2-413[»]
2GFYX-ray2.85A2-413[»]
3G0YX-ray2.60A2-413[»]
3G11X-ray2.00A2-413[»]
3HNZX-ray2.75A2-413[»]
3HO2X-ray2.00A2-413[»]
3HO9X-ray1.90A2-413[»]
3I8PX-ray1.90A2-413[»]
ProteinModelPortaliP0AAI5.
SMRiP0AAI5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260072. 201 interactors.
DIPiDIP-29377N.
IntActiP0AAI5. 6 interactors.
MINTiMINT-1231688.
STRINGi511145.b1095.

Chemistry databases

DrugBankiDB01034. Cerulenin.

Proteomic databases

EPDiP0AAI5.
PaxDbiP0AAI5.
PRIDEiP0AAI5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74179; AAC74179; b1095.
BAA35903; BAA35903; BAA35903.
GeneIDi946665.
KEGGiecj:JW1081.
eco:b1095.
PATRICi32117431. VBIEscCol129921_1138.

Organism-specific databases

EchoBASEiEB2490.
EcoGeneiEG12606. fabF.

Phylogenomic databases

eggNOGiENOG4105C0Q. Bacteria.
COG0304. LUCA.
HOGENOMiHOG000060165.
InParanoidiP0AAI5.
KOiK09458.
OMAiSIKHGFM.
PhylomeDBiP0AAI5.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciEcoCyc:3-OXOACYL-ACP-SYNTHII-MONOMER.
ECOL316407:JW1081-MONOMER.
MetaCyc:3-OXOACYL-ACP-SYNTHII-MONOMER.
BRENDAi2.3.1.179. 2026.
SABIO-RKP0AAI5.

Miscellaneous databases

EvolutionaryTraceiP0AAI5.
PROiP0AAI5.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000447. KAS_II. 1 hit.
SMARTiSM00825. PKS_KS. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFABF_ECOLI
AccessioniPrimary (citable) accession number: P0AAI5
Secondary accession number(s): P39435
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.