P0AAI1 (SSUB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aliphatic sulfonates import ATP-binding protein SsuB
EC=3.6.3.-

Gene names

Name:	ssuB
Synonyms:	ycbE
Ordered Locus Names:	b0933, JW0916

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex SsuABC involved in aliphatic sulfonates import. Responsible for energy coupling to the transport system Probable. Ref.1 Ref.7
Subunit structure	The complex is composed of two ATP-binding proteins (SsuB), two transmembrane proteins (SsuC) and a solute-binding protein (SsuA) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Induction	Repressed by sulfate and cystine. Transcriptionally regulated by cbl. Ref.1
Sequence similarities	Belongs to the ABC transporter superfamily. Aliphatic sulfonates importer (TC 3.A.1.17.2) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC alkanesulfonate transport Inferred from mutant phenotype Ref.1. Source: EcoCyc
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from electronic annotation. Source: InterPro transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Aliphatic sulfonates import ATP-binding protein SsuB		PRO_0000092976
Regions
	Domain	12 – 233	222	ABC transporter
	Nucleotide binding	44 – 51	8	ATP Potential
Experimental info
	Sequence conflict	161 – 165	5	GALDA → RGAGR in M15273. Ref.5
	Sequence conflict	232 – 233	2	EL → DV in M15273. Ref.5
	Sequence conflict	236 – 237	2	EV → RS in M15273. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P0AAI1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 6FA276AD6E7FACE1	FASTA	255	27,738
	10 20 30 40 50 60 MNTARLNQGT PLLLNAVSKH YAENIVLNQL DLHIPAGQFV AVVGRSGGGK STLLRLLAGL 70 80 90 100 110 120 ETPTAGDVLA GTTPLAEIQE DTRMMFQDAR LLPWKSVIDN VGLGLKGQWR DAARRALAAV 130 140 150 160 170 180 GLENRAGEWP AALSGGQKQR VALARALIHR PGLLLLDEPL GALDALTRLE MQDLIVSLWQ 190 200 210 220 230 240 EHGFTVLLVT HDVSEAVAMA DRVLLIEEGK IGLDLTVDIP RPRRLGSVRL AELEAEVLQR 250 VMQRGESETR LRKQG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Escherichia coli ssuEADCB gene cluster is required for the utilization of sulfur from aliphatic sulfonates and is regulated by the transcriptional activator Cbl." Van der Ploeg J.R., Iwanicka-Nowicka R., Bykowski T., Hryniewicz M.M., Leisinger T. J. Biol. Chem. 274:29358-29365(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN ALIPHATIC SULFONATES TRANSPORT, INDUCTION. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The nucleotide sequence of the pepN gene and its over-expression in Escherichia coli." McCaman M.T., Gabe J.D. Gene 48:145-153(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 161-255. Strain: K12.
[6]	"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Borodovsky M., Rudd K.E., Koonin E.V. Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[7]	"Deletion analysis of the Escherichia coli taurine and alkanesulfonate transport systems." Eichhorn E., van der Ploeg J.R., Leisinger T. J. Bacteriol. 182:2687-2695(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN ALIPHATIC SULFONATES TRANSPORT. Strain: K12.
[8]	"Sulfonate-sulfur metabolism and its regulation in Escherichia coli." van der Ploeg J.R., Eichhorn E., Leisinger T. Arch. Microbiol. 176:1-8(2001) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ237695 Genomic DNA. Translation: CAB40393.1. U00096 Genomic DNA. Translation: AAC74019.1. AP009048 Genomic DNA. Translation: BAA35685.1. M15273 Genomic DNA. No translation available.
PIR	D64833.
RefSeq	NP_415453.1. NC_000913.2. YP_489205.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AAI1.
SMR	P0AAI1. Positions 6-246.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AAI1. 6 interactions.
STRING	511145.b0933.
Proteomic databases
PaxDb	P0AAI1.
PRIDE	P0AAI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74019; AAC74019; b0933. BAA35685; BAA35685; BAA35685.
GeneID	12932885. 947220.
KEGG	ecj:Y75_p0905. eco:b0933.
PATRIC	32117085. VBIEscCol129921_0967.
Organism-specific databases
EchoBASE	EB2261.
EcoGene	EG12358. ssuB.
Phylogenomic databases
eggNOG	COG1116.
KO	K15555.
OMA	DIRLMFQ.
ProtClustDB	PRK11247.
Enzyme and pathway databases
BioCyc	EcoCyc:YCBE-MONOMER. ECOL316407:JW0916-MONOMER.
Gene expression databases
Genevestigator	P0AAI1.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017875. ABC_transptr_sulphonate_ATP-bd. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51291. SSUB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSUB_ECOLI

Accession

Primary (citable) accession number: P0AAI1
Secondary accession number(s): P38053, P75850

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program