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Protein

Putrescine export system ATP-binding protein SapF

Gene

sapF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of a putrescine export transport system, does not play a role in resistance to antimicrobial peptides (PubMed:27803167). Does not stimulate K+ uptake ability of TrkH on its own, but increases K+ uptake by 20% in the presence of SapD; has no effect of TrkG (PubMed:11700350).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 54ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:SAPF-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.42. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine export system ATP-binding protein SapF1 Publication
Gene namesi
Name:sapF
Ordered Locus Names:b1290, JW1283
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12305. sapF.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Single mutation, decreased extracellular putrescine, in an sapBCDF operon deletion no change in resistance to antimicrobial peptide LL-37.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000929681 – 268Putrescine export system ATP-binding protein SapFAdd BLAST268

Proteomic databases

PaxDbiP0AAH8.
PRIDEiP0AAH8.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ybiTP0A9U32EBI-558765,EBI-558999

Protein-protein interaction databases

BioGridi4263241. 403 interactors.
DIPiDIP-35903N.
IntActiP0AAH8. 10 interactors.
STRINGi511145.b1290.

Structurei

3D structure databases

ProteinModelPortaliP0AAH8.
SMRiP0AAH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 251ABC transporterPROSITE-ProRule annotationAdd BLAST246

Sequence similaritiesi

Belongs to the ABC transporter superfamily.Curated

Phylogenomic databases

eggNOGiENOG4108JQ2. Bacteria.
COG4167. LUCA.
InParanoidiP0AAH8.
KOiK19230.
PhylomeDBiP0AAH8.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00005. ABC_tran. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AAH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETLLEVRN LSKTFRYRTG WFRRQTVEAV KPLSFTLREG QTLAIIGENG
60 70 80 90 100
SGKSTLAKML AGMIEPTSGE LLIDDHPLHF GDYSFRSQRI RMIFQDPSTS
110 120 130 140 150
LNPRQRISQI LDFPLRLNTD LEPEQRRKQI IETMRMVGLL PDHVSYYPHM
160 170 180 190 200
LAPGQKQRLG LARALILRPK VIIADEALAS LDMSMRSQLI NLMLELQEKQ
210 220 230 240 250
GISYIYVTQH IGMMKHISDQ VLVMHQGEVV ERGSTADVLA SPLHELTKRL
260
IAGHFGEALT ADAWRKDR
Length:268
Mass (Da):30,571
Last modified:October 11, 2005 - v1
Checksum:iF3B98BC9DC6DCBFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08190 Unassigned DNA. Translation: AAA17671.1.
X97282 Genomic DNA. Translation: CAA65941.1.
U00096 Genomic DNA. Translation: AAC74372.1.
AP009048 Genomic DNA. Translation: BAA14843.1.
PIRiE64877.
RefSeqiNP_415806.1. NC_000913.3.
WP_000573407.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74372; AAC74372; b1290.
BAA14843; BAA14843; BAA14843.
GeneIDi945335.
KEGGiecj:JW1283.
eco:b1290.
PATRICifig|1411691.4.peg.989.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08190 Unassigned DNA. Translation: AAA17671.1.
X97282 Genomic DNA. Translation: CAA65941.1.
U00096 Genomic DNA. Translation: AAC74372.1.
AP009048 Genomic DNA. Translation: BAA14843.1.
PIRiE64877.
RefSeqiNP_415806.1. NC_000913.3.
WP_000573407.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAH8.
SMRiP0AAH8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263241. 403 interactors.
DIPiDIP-35903N.
IntActiP0AAH8. 10 interactors.
STRINGi511145.b1290.

Protein family/group databases

TCDBi3.A.1.5.42. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AAH8.
PRIDEiP0AAH8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74372; AAC74372; b1290.
BAA14843; BAA14843; BAA14843.
GeneIDi945335.
KEGGiecj:JW1283.
eco:b1290.
PATRICifig|1411691.4.peg.989.

Organism-specific databases

EchoBASEiEB2211.
EcoGeneiEG12305. sapF.

Phylogenomic databases

eggNOGiENOG4108JQ2. Bacteria.
COG4167. LUCA.
InParanoidiP0AAH8.
KOiK19230.
PhylomeDBiP0AAH8.

Enzyme and pathway databases

BioCyciEcoCyc:SAPF-MONOMER.

Miscellaneous databases

PROiPR:P0AAH8.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00005. ABC_tran. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSAPF_ECOLI
AccessioniPrimary (citable) accession number: P0AAH8
Secondary accession number(s): P36637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: June 7, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.