sapF

Functionⁱ

Part of a putrescine export transport system, does not play a role in resistance to antimicrobial peptides (PubMed:27803167). Does not stimulate K⁺ uptake ability of TrkH on its own, but increases K⁺ uptake by 20% in the presence of SapD; has no effect of TrkG (PubMed:11700350).2 Publications

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	47 – 54	ATPPROSITE-ProRule annotation		8

GO - Molecular functionⁱ

ATPase activity Source: InterPro
ATP binding Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

transport Source: UniProtKB-KW

Complete GO annotation...

Keywordsⁱ

Biological process	Transport
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:SAPF-MONOMER.

BioCycⁱ

EcoCyc:SAPF-MONOMER.

Protein family/group databases

TCDBⁱ	3.A.1.5.42. the atp-binding cassette (abc) superfamily.

TCDBⁱ

3.A.1.5.42. the atp-binding cassette (abc) superfamily.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Putrescine export system ATP-binding protein SapF1 Publication
Gene namesⁱ	Name:sapF Ordered Locus Names:b1290, JW1283
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG12305. sapF.

EcoGeneⁱ

EG12305. sapF.

Subcellular locationⁱ

Cell inner membrane Curated; Peripheral membrane protein Curated

GO - Cellular componentⁱ

plasma membrane Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Single mutation, decreased extracellular putrescine, in an sapBCDF operon deletion no change in resistance to antimicrobial peptide LL-37.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000092968	1 – 268	Putrescine export system ATP-binding protein SapFAdd BLAST		268

Proteomic databases

PaxDbⁱ	P0AAH8.
PRIDEⁱ	P0AAH8.

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
ybiT	P0A9U3	2	EBI-558765,EBI-558999

With

Entry

#Exp.

IntAct

Notes

ybiT

P0A9U3

2

EBI-558765,EBI-558999

Protein-protein interaction databases

BioGridⁱ	4263241. 403 interactors.
Database of interacting proteins More...DIPⁱ	DIP-35903N.
IntActⁱ	P0AAH8. 10 interactors.
STRINGⁱ	316385.ECDH10B_1407.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0AAH8.
SMRⁱ	P0AAH8.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	6 – 251	ABC transporterPROSITE-ProRule annotationAdd BLAST		246

Sequence similaritiesⁱ

Belongs to the ABC transporter superfamily.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4108JQ2. Bacteria. COG4167. LUCA.
InParanoidⁱ	P0AAH8.
KEGG Orthology (KO) More...KOⁱ	K19230.
PhylomeDBⁱ	P0AAH8.

Family and domain databases

InterProⁱ	View protein in InterPro IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR027417. P-loop_NTPase.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00005. ABC_tran. 1 hit.
SMARTⁱ	View protein in SMART SM00382. AAA. 1 hit.
SUPFAMⁱ	SSF52540. SSF52540. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AAH8-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIETLLEVRN LSKTFRYRTG WFRRQTVEAV KPLSFTLREG QTLAIIGENG 
        60         70         80         90        100
SGKSTLAKML AGMIEPTSGE LLIDDHPLHF GDYSFRSQRI RMIFQDPSTS 
       110        120        130        140        150
LNPRQRISQI LDFPLRLNTD LEPEQRRKQI IETMRMVGLL PDHVSYYPHM 
       160        170        180        190        200
LAPGQKQRLG LARALILRPK VIIADEALAS LDMSMRSQLI NLMLELQEKQ 
       210        220        230        240        250
GISYIYVTQH IGMMKHISDQ VLVMHQGEVV ERGSTADVLA SPLHELTKRL 
       260 
IAGHFGEALT ADAWRKDR

Length:268

Mass (Da):30,571

Last modified:October 11, 2005 - v1

Checksum:ⁱF3B98BC9DC6DCBFA

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U08190 Unassigned DNA. Translation: AAA17671.1. X97282 Genomic DNA. Translation: CAA65941.1. U00096 Genomic DNA. Translation: AAC74372.1. AP009048 Genomic DNA. Translation: BAA14843.1.
PIRⁱ	E64877.
NCBI Reference Sequences More...RefSeqⁱ	NP_415806.1. NC_000913.3. WP_000573407.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAC74372; AAC74372; b1290. BAA14843; BAA14843; BAA14843.
GeneIDⁱ	945335.
KEGGⁱ	ecj:JW1283. eco:b1290.
PATRICⁱ	fig\|1411691.4.peg.989.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U08190 Unassigned DNA. Translation: AAA17671.1. X97282 Genomic DNA. Translation: CAA65941.1. U00096 Genomic DNA. Translation: AAC74372.1. AP009048 Genomic DNA. Translation: BAA14843.1.
PIRⁱ	E64877.
NCBI Reference Sequences More...RefSeqⁱ	NP_415806.1. NC_000913.3. WP_000573407.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortalⁱ	P0AAH8.
SMRⁱ	P0AAH8.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263241. 403 interactors.
Database of interacting proteins More...DIPⁱ	DIP-35903N.
IntActⁱ	P0AAH8. 10 interactors.
STRINGⁱ	316385.ECDH10B_1407.

Protein family/group databases

TCDBⁱ	3.A.1.5.42. the atp-binding cassette (abc) superfamily.

TCDBⁱ

3.A.1.5.42. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbⁱ	P0AAH8.
PRIDEⁱ	P0AAH8.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74372; AAC74372; b1290. BAA14843; BAA14843; BAA14843.
GeneIDⁱ	945335.
KEGGⁱ	ecj:JW1283. eco:b1290.
PATRICⁱ	fig\|1411691.4.peg.989.

Organism-specific databases

EchoBASEⁱ	EB2211.
EcoGeneⁱ	EG12305. sapF.

Phylogenomic databases

eggNOGⁱ	ENOG4108JQ2. Bacteria. COG4167. LUCA.
InParanoidⁱ	P0AAH8.
KEGG Orthology (KO) More...KOⁱ	K19230.
PhylomeDBⁱ	P0AAH8.

Enzyme and pathway databases

BioCycⁱ	EcoCyc:SAPF-MONOMER.

BioCycⁱ

EcoCyc:SAPF-MONOMER.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0AAH8.

PROⁱ

PR:P0AAH8.

Family and domain databases

InterProⁱ	View protein in InterPro IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR027417. P-loop_NTPase.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00005. ABC_tran. 1 hit.
SMARTⁱ	View protein in SMART SM00382. AAA. 1 hit.
SUPFAMⁱ	SSF52540. SSF52540. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SAPF_ECOLI
Accessionⁱ	P0AAH8Primary (citable) accession number: P0AAH8 Secondary accession number(s): P36637
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
	Last sequence update:	October 11, 2005
	Last modified:	July 5, 2017
	This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0AAH8 (SAPF_ECOLI)

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Putrescine export system ATP-binding protein SapF

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ