P0AAH8 (SAPF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide transport system ATP-binding protein SapF

Gene names

Name:	sapF
Ordered Locus Names:	b1290, JW1283

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides.
Subcellular location	Cell inner membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the ABC transporter superfamily. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Peptide transport Protein transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC peptide transport Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Peptide transport system ATP-binding protein SapF		PRO_0000092968
Regions
	Domain	6 – 251	246	ABC transporter
	Nucleotide binding	47 – 54	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AAH8 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: F3B98BC9DC6DCBFA	FASTA	268	30,571
	10 20 30 40 50 60 MIETLLEVRN LSKTFRYRTG WFRRQTVEAV KPLSFTLREG QTLAIIGENG SGKSTLAKML 70 80 90 100 110 120 AGMIEPTSGE LLIDDHPLHF GDYSFRSQRI RMIFQDPSTS LNPRQRISQI LDFPLRLNTD 130 140 150 160 170 180 LEPEQRRKQI IETMRMVGLL PDHVSYYPHM LAPGQKQRLG LARALILRPK VIIADEALAS 190 200 210 220 230 240 LDMSMRSQLI NLMLELQEKQ GISYIYVTQH IGMMKHISDQ VLVMHQGEVV ERGSTADVLA 250 260 SPLHELTKRL IAGHFGEALT ADAWRKDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Bergler H., Ebeling A., Fuchsbichler S., Hogenauer G., Turnowsky F. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Epstein W., Noelker E., Stumpe S., Tewes R., Schmid R., Bakker E.P. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U08190 Unassigned DNA. Translation: AAA17671.1. X97282 Genomic DNA. Translation: CAA65941.1. U00096 Genomic DNA. Translation: AAC74372.1. AP009048 Genomic DNA. Translation: BAA14843.1.
PIR	E64877.
RefSeq	NP_415806.1. NC_000913.2. YP_489558.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AAH8.
SMR	P0AAH8. Positions 27-242.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-35903N.
IntAct	P0AAH8. 9 interactions.
STRING	511145.b1290.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74372; AAC74372; b1290. BAA14843; BAA14843; BAA14843.
GeneID	12934247. 945335.
KEGG	ecj:Y75_p1265. eco:b1290.
PATRIC	32117850. VBIEscCol129921_1345.
Organism-specific databases
EchoBASE	EB2211.
EcoGene	EG12305. sapF.
Phylogenomic databases
eggNOG	COG4167.
KO	K12372.
OMA	YRYRSQR.
ProtClustDB	PRK15112.
Enzyme and pathway databases
BioCyc	EcoCyc:SAPF-MONOMER. ECOL316407:JW1283-MONOMER.
Gene expression databases
Genevestigator	P0AAH8.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAPF_ECOLI

Accession

Primary (citable) accession number: P0AAH8
Secondary accession number(s): P36637

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program