UniProtKB - P0AAH8 (SAPF_ECOLI)
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Protein
Putrescine export system ATP-binding protein SapF
Gene
sapF
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of a putrescine export transport system, does not play a role in resistance to antimicrobial peptides (PubMed:27803167). Does not stimulate K+ uptake ability of TrkH on its own, but increases K+ uptake by 20% in the presence of SapD; has no effect of TrkG (PubMed:11700350).2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 47 – 54 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATP binding Source: EcoCyc
- putrescine transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- putrescine transport Source: EcoCyc
Keywordsi
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:SAPF-MONOMER. MetaCyc:SAPF-MONOMER. |
Protein family/group databases
TCDBi | 3.A.1.5.42. the atp-binding cassette (abc) superfamily. |
Names & Taxonomyi
Protein namesi | Recommended name: Putrescine export system ATP-binding protein SapF1 Publication |
Gene namesi | Name:sapF Ordered Locus Names:b1290, JW1283 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG12305. sapF. |
Subcellular locationi
- Cell inner membrane Curated; Peripheral membrane protein Curated
GO - Cellular componenti
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Single mutation, decreased extracellular putrescine, in an sapBCDF operon deletion no change in resistance to antimicrobial peptide LL-37.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092968 | 1 – 268 | Putrescine export system ATP-binding protein SapFAdd BLAST | 268 |
Proteomic databases
PaxDbi | P0AAH8. |
PRIDEi | P0AAH8. |
Interactioni
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
ybiT | P0A9U3 | 2 | EBI-558765,EBI-558999 |
Protein-protein interaction databases
BioGridi | 4263241. 410 interactors. |
DIPi | DIP-35903N. |
IntActi | P0AAH8. 10 interactors. |
STRINGi | 316385.ECDH10B_1407. |
Structurei
3D structure databases
ProteinModelPortali | P0AAH8. |
SMRi | P0AAH8. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 251 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 246 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily.Curated
Phylogenomic databases
eggNOGi | ENOG4108JQ2. Bacteria. COG4167. LUCA. |
InParanoidi | P0AAH8. |
KOi | K19230. |
OMAi | CKLLRMI. |
PhylomeDBi | P0AAH8. |
Family and domain databases
InterProi | View protein in InterPro IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR027417. P-loop_NTPase. |
Pfami | View protein in Pfam PF00005. ABC_tran. 1 hit. |
SMARTi | View protein in SMART SM00382. AAA. 1 hit. |
SUPFAMi | SSF52540. SSF52540. 1 hit. |
PROSITEi | View protein in PROSITE PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. |
i Sequence
Sequence statusi: Complete.
P0AAH8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIETLLEVRN LSKTFRYRTG WFRRQTVEAV KPLSFTLREG QTLAIIGENG
60 70 80 90 100
SGKSTLAKML AGMIEPTSGE LLIDDHPLHF GDYSFRSQRI RMIFQDPSTS
110 120 130 140 150
LNPRQRISQI LDFPLRLNTD LEPEQRRKQI IETMRMVGLL PDHVSYYPHM
160 170 180 190 200
LAPGQKQRLG LARALILRPK VIIADEALAS LDMSMRSQLI NLMLELQEKQ
210 220 230 240 250
GISYIYVTQH IGMMKHISDQ VLVMHQGEVV ERGSTADVLA SPLHELTKRL
260
IAGHFGEALT ADAWRKDR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08190 Unassigned DNA. Translation: AAA17671.1. X97282 Genomic DNA. Translation: CAA65941.1. U00096 Genomic DNA. Translation: AAC74372.1. AP009048 Genomic DNA. Translation: BAA14843.1. |
PIRi | E64877. |
RefSeqi | NP_415806.1. NC_000913.3. WP_000573407.1. NZ_LN832404.1. |
Genome annotation databases
EnsemblBacteriai | AAC74372; AAC74372; b1290. BAA14843; BAA14843; BAA14843. |
GeneIDi | 945335. |
KEGGi | ecj:JW1283. eco:b1290. |
PATRICi | fig|1411691.4.peg.989. |
Similar proteinsi
Entry informationi
Entry namei | SAPF_ECOLI | |
Accessioni | P0AAH8Primary (citable) accession number: P0AAH8 Secondary accession number(s): P36637 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | October 11, 2005 | |
Last modified: | March 28, 2018 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |