Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putrescine export system ATP-binding protein SapD

Gene

sapD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of a putrescine export transport system, does not play a role in resistance to antimicrobial peptides (PubMed:27803167). Stimulates K+-uptake proteins TrkG and TrkH to import K+, may act via ATP-binding rather than ATP hydrolysis (PubMed:11700350).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 47ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: EcoCyc

GO - Biological processi

  • peptide transport Source: InterPro
  • potassium ion transmembrane transport Source: EcoCyc

Keywordsi

Biological processIon transport, Potassium transport, Transport
LigandATP-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciEcoCyc:SAPD-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.42. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine export system ATP-binding protein SapD1 Publication
Gene namesi
Name:sapD
Synonyms:trkE1 Publication
Ordered Locus Names:b1291, JW1284
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12304. sapD.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Single mutation, decreased extracellular putrescine, in an sapBCDF operon deletion no change in resistance to antimicrobial peptide LL-37.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi46K → G, I, Q or R: 25% to 50% stimulation of K(+) uptake by TrkH. 1 Publication1
Mutagenesisi46Missing : Almost no stimulation of K(+) uptake by TrkH. 1 Publication1
Mutagenesisi162G → A: 80% stimulation of K(+) uptake by TrkH. 1 Publication1
Mutagenesisi165Q → L: 60% stimulation of K(+) uptake by TrkH. 1 Publication1
Mutagenesisi183D → E: About 60% stimulation of K(+) uptake by TrkH. 1 Publication1
Mutagenesisi183D → K or N: 15% to 20% stimulation of K(+) uptake by TrkH. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000929621 – 330Putrescine export system ATP-binding protein SapDAdd BLAST330

Proteomic databases

PaxDbiP0AAH4.
PRIDEiP0AAH4.

Interactioni

Protein-protein interaction databases

BioGridi4260128. 348 interactors.
DIPiDIP-10825N.
IntActiP0AAH4. 5 interactors.
MINTiMINT-1271696.
STRINGi511145.b1291.

Structurei

3D structure databases

ProteinModelPortaliP0AAH4.
SMRiP0AAH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 259ABC transporterPROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Belongs to the ABC transporter superfamily.Curated

Phylogenomic databases

eggNOGiENOG4108JQ3. Bacteria.
COG4170. LUCA.
InParanoidiP0AAH4.
KOiK19229.
OMAiYCGQMVE.
PhylomeDBiP0AAH4.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR013563. Oligopep_ABC_C.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00005. ABC_tran. 1 hit.
PF08352. oligo_HPY. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01727. oligo_HPY. 1 hit.
PROSITEiView protein in PROSITE
PS50893. ABC_TRANSPORTER_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AAH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLDIRNLT IEFKTGDEWV KAVDRVSMTL TEGEIRGLVG ESGSGKSLIA
60 70 80 90 100
KAICGVNKDN WRVTADRMRF DDIDLLRLSA RERRKLVGHN VSMIFQEPQS
110 120 130 140 150
CLDPSERVGR QLMQNIPAWT YKGRWWQRFG WRKRRAIELL HRVGIKDHKD
160 170 180 190 200
AMRSFPYELT EGECQKVMIA IALANQPRLL IADEPTNSME PTTQAQIFRL
210 220 230 240 250
LTRLNQNSNT TILLISHDLQ MLSQWADKIN VLYCGQTVET APSKELVTMP
260 270 280 290 300
HHPYTQALIR AIPDFGSAMP HKSRLNTLPG AIPLLEQLPI GCRLGPRCPY
310 320 330
AQRECIVTPR LTGAKNHLYA CHFPLNMEKE
Length:330
Mass (Da):37,661
Last modified:October 11, 2005 - v1
Checksum:i1326280E475628E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97282 Genomic DNA. Translation: CAA65940.1.
U00096 Genomic DNA. Translation: AAC74373.1.
AP009048 Genomic DNA. Translation: BAA14844.1.
U08190 Unassigned DNA. Translation: AAA17670.1.
PIRiF64877.
RefSeqiNP_415807.1. NC_000913.3.
WP_001128858.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74373; AAC74373; b1291.
BAA14844; BAA14844; BAA14844.
GeneIDi946203.
KEGGiecj:JW1284.
eco:b1291.
PATRICi32117852. VBIEscCol129921_1346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97282 Genomic DNA. Translation: CAA65940.1.
U00096 Genomic DNA. Translation: AAC74373.1.
AP009048 Genomic DNA. Translation: BAA14844.1.
U08190 Unassigned DNA. Translation: AAA17670.1.
PIRiF64877.
RefSeqiNP_415807.1. NC_000913.3.
WP_001128858.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAH4.
SMRiP0AAH4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260128. 348 interactors.
DIPiDIP-10825N.
IntActiP0AAH4. 5 interactors.
MINTiMINT-1271696.
STRINGi511145.b1291.

Protein family/group databases

TCDBi3.A.1.5.42. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AAH4.
PRIDEiP0AAH4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74373; AAC74373; b1291.
BAA14844; BAA14844; BAA14844.
GeneIDi946203.
KEGGiecj:JW1284.
eco:b1291.
PATRICi32117852. VBIEscCol129921_1346.

Organism-specific databases

EchoBASEiEB2210.
EcoGeneiEG12304. sapD.

Phylogenomic databases

eggNOGiENOG4108JQ3. Bacteria.
COG4170. LUCA.
InParanoidiP0AAH4.
KOiK19229.
OMAiYCGQMVE.
PhylomeDBiP0AAH4.

Enzyme and pathway databases

BioCyciEcoCyc:SAPD-MONOMER.

Miscellaneous databases

PROiPR:P0AAH4.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR013563. Oligopep_ABC_C.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00005. ABC_tran. 1 hit.
PF08352. oligo_HPY. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01727. oligo_HPY. 1 hit.
PROSITEiView protein in PROSITE
PS50893. ABC_TRANSPORTER_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSAPD_ECOLI
AccessioniPrimary (citable) accession number: P0AAH4
Secondary accession number(s): P36635
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: April 12, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.