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Protein

Phosphate import ATP-binding protein PstB

Gene

pstB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.UniRule annotation

Catalytic activityi

ATP + H2O + phosphate(Out) = ADP + phosphate + phosphate(In).UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 50ATPUniRule annotation8

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Phosphate transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOO157:PSTB-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate import ATP-binding protein PstBUniRule annotation (EC:3.6.3.27UniRule annotation)
Alternative name(s):
ABC phosphate transporterUniRule annotation
Phosphate-transporting ATPaseUniRule annotation
Gene namesi
Name:pstBUniRule annotation
Ordered Locus Names:Z5216, ECs4661
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000928112 – 257Phosphate import ATP-binding protein PstBAdd BLAST256

Proteomic databases

PRIDEiP0AAH2.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).UniRule annotation

Protein-protein interaction databases

MINTiMINT-1233544.
STRINGi155864.Z5216.

Structurei

3D structure databases

ProteinModelPortaliP0AAH2.
SMRiP0AAH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 252ABC transporterUniRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZY. Bacteria.
COG1117. LUCA.
KOiK02036.
OMAiMTVEPRS.

Family and domain databases

CDDicd03260. ABC_PstB_phosphate_transporter. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015850. ABC_transpr_PstB.
IPR027417. P-loop_NTPase.
IPR005670. Phosp_transpt1.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00972. 3a0107s01c2. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51238. PSTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAH2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMVETAPSK IQVRNLNFYY GKFHALKNIN LDIAKNQVTA FIGPSGCGKS
60 70 80 90 100
TLLRTFNKMF ELYPEQRAEG EILLDGDNIL TNSQDIALLR AKVGMVFQKP
110 120 130 140 150
TPFPMSIYDN IAFGVRLFEK LSRADMDERV QWALTKAALW NETKDKLHQS
160 170 180 190 200
GYSLSGGQQQ RLCIARGIAI RPEVLLLDEP CSALDPISTG RIEELITELK
210 220 230 240 250
QDYTVVIVTH NMQQAARCSD HTAFMYLGEL IEFSNTDDLF TKPAKKQTED

YITGRYG
Length:257
Mass (Da):29,027
Last modified:January 23, 2007 - v2
Checksum:i15A09F9DC3EB013A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58921.1.
BA000007 Genomic DNA. Translation: BAB38084.1.
PIRiE86057.
E91211.
RefSeqiNP_312688.1. NC_002695.1.
WP_000063125.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG58921; AAG58921; Z5216.
BAB38084; BAB38084; BAB38084.
GeneIDi915370.
KEGGiece:Z5216.
ecs:ECs4661.
PATRICi18358919. VBIEscCol44059_4624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58921.1.
BA000007 Genomic DNA. Translation: BAB38084.1.
PIRiE86057.
E91211.
RefSeqiNP_312688.1. NC_002695.1.
WP_000063125.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0AAH2.
SMRiP0AAH2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1233544.
STRINGi155864.Z5216.

Proteomic databases

PRIDEiP0AAH2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58921; AAG58921; Z5216.
BAB38084; BAB38084; BAB38084.
GeneIDi915370.
KEGGiece:Z5216.
ecs:ECs4661.
PATRICi18358919. VBIEscCol44059_4624.

Phylogenomic databases

eggNOGiENOG4105BZY. Bacteria.
COG1117. LUCA.
KOiK02036.
OMAiMTVEPRS.

Enzyme and pathway databases

BioCyciECOO157:PSTB-MONOMER.

Family and domain databases

CDDicd03260. ABC_PstB_phosphate_transporter. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015850. ABC_transpr_PstB.
IPR027417. P-loop_NTPase.
IPR005670. Phosp_transpt1.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00972. 3a0107s01c2. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51238. PSTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSTB_ECO57
AccessioniPrimary (citable) accession number: P0AAH2
Secondary accession number(s): P07655
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.