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Protein

Phosphate import ATP-binding protein PstB

Gene

pstB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.

Catalytic activityi

ATP + H2O + phosphate(Out) = ADP + phosphate + phosphate(In).UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 508ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • phosphate ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Phosphate transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PSTB-MONOMER.
ECOL316407:JW3703-MONOMER.
MetaCyc:PSTB-MONOMER.

Protein family/group databases

TCDBi3.A.1.7.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate import ATP-binding protein PstBUniRule annotation (EC:3.6.3.27UniRule annotation)
Alternative name(s):
ABC phosphate transporterUniRule annotation
Phosphate-transporting ATPaseUniRule annotation
Gene namesi
Name:pstBUniRule annotation
Synonyms:phoT
Ordered Locus Names:b3725, JW3703
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10783. pstB.

Subcellular locationi

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 481G → I: Loss of phosphate transport. 1 Publication
Mutagenesisi49 – 491K → Q: Loss of phosphate transport. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 257256Phosphate import ATP-binding protein PstBPRO_0000092810Add
BLAST

Proteomic databases

PaxDbiP0AAH0.
PRIDEiP0AAH0.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).UniRule annotation

Protein-protein interaction databases

BioGridi4262135. 10 interactions.
DIPiDIP-47851N.
IntActiP0AAH0. 26 interactions.
MINTiMINT-1233567.
STRINGi511145.b3725.

Structurei

3D structure databases

ProteinModelPortaliP0AAH0.
SMRiP0AAH0. Positions 9-253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 252242ABC transporterUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZY. Bacteria.
COG1117. LUCA.
InParanoidiP0AAH0.
KOiK02036.
OMAiMTVEPRS.
OrthoDBiEOG6T7N3V.
PhylomeDBiP0AAH0.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015850. ABC_transpr_PstB.
IPR027417. P-loop_NTPase.
IPR005670. Phosp_transpt1.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00972. 3a0107s01c2. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51238. PSTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AAH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMVETAPSK IQVRNLNFYY GKFHALKNIN LDIAKNQVTA FIGPSGCGKS
60 70 80 90 100
TLLRTFNKMF ELYPEQRAEG EILLDGDNIL TNSQDIALLR AKVGMVFQKP
110 120 130 140 150
TPFPMSIYDN IAFGVRLFEK LSRADMDERV QWALTKAALW NETKDKLHQS
160 170 180 190 200
GYSLSGGQQQ RLCIARGIAI RPEVLLLDEP CSALDPISTG RIEELITELK
210 220 230 240 250
QDYTVVIVTH NMQQAARCSD HTAFMYLGEL IEFSNTDDLF TKPAKKQTED

YITGRYG
Length:257
Mass (Da):29,027
Last modified:January 23, 2007 - v2
Checksum:i15A09F9DC3EB013A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02723 Genomic DNA. Translation: CAA26509.1.
K01992 Genomic DNA. Translation: AAA24381.1.
L10328 Genomic DNA. Translation: AAA62076.1.
U00096 Genomic DNA. Translation: AAC76748.1.
AP009048 Genomic DNA. Translation: BAE77563.1.
PIRiA30382. BVECZB.
RefSeqiNP_418181.1. NC_000913.3.
WP_000063125.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76748; AAC76748; b3725.
BAE77563; BAE77563; BAE77563.
GeneIDi948240.
KEGGiecj:JW3703.
eco:b3725.
PATRICi32122947. VBIEscCol129921_3849.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02723 Genomic DNA. Translation: CAA26509.1.
K01992 Genomic DNA. Translation: AAA24381.1.
L10328 Genomic DNA. Translation: AAA62076.1.
U00096 Genomic DNA. Translation: AAC76748.1.
AP009048 Genomic DNA. Translation: BAE77563.1.
PIRiA30382. BVECZB.
RefSeqiNP_418181.1. NC_000913.3.
WP_000063125.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAH0.
SMRiP0AAH0. Positions 9-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262135. 10 interactions.
DIPiDIP-47851N.
IntActiP0AAH0. 26 interactions.
MINTiMINT-1233567.
STRINGi511145.b3725.

Protein family/group databases

TCDBi3.A.1.7.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AAH0.
PRIDEiP0AAH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76748; AAC76748; b3725.
BAE77563; BAE77563; BAE77563.
GeneIDi948240.
KEGGiecj:JW3703.
eco:b3725.
PATRICi32122947. VBIEscCol129921_3849.

Organism-specific databases

EchoBASEiEB0776.
EcoGeneiEG10783. pstB.

Phylogenomic databases

eggNOGiENOG4105BZY. Bacteria.
COG1117. LUCA.
InParanoidiP0AAH0.
KOiK02036.
OMAiMTVEPRS.
OrthoDBiEOG6T7N3V.
PhylomeDBiP0AAH0.

Enzyme and pathway databases

BioCyciEcoCyc:PSTB-MONOMER.
ECOL316407:JW3703-MONOMER.
MetaCyc:PSTB-MONOMER.

Miscellaneous databases

PROiP0AAH0.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015850. ABC_transpr_PstB.
IPR027417. P-loop_NTPase.
IPR005670. Phosp_transpt1.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00972. 3a0107s01c2. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51238. PSTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli."
    Amemura M., Makino K., Shinagawa H., Kobayashi A., Nakata A.
    J. Mol. Biol. 184:241-250(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships."
    Surin B.P., Rosenberg H., Cox G.B.
    J. Bacteriol. 161:189-198(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "PstB protein of the phosphate-specific transport system of Escherichia coli is an ATPase."
    Chan F.-Y., Torriani A.
    J. Bacteriol. 178:3974-3977(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION, MUTAGENESIS OF GLY-48 AND LYS-49.
    Strain: BL21-DE3.

Entry informationi

Entry nameiPSTB_ECOLI
AccessioniPrimary (citable) accession number: P0AAH0
Secondary accession number(s): P07655, Q2M843
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.