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Protein

Galactose/methyl galactoside import ATP-binding protein MglA

Gene

mglA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MglABC involved in galactose/methyl galactoside import. Responsible for energy coupling to the transport system (Probable).3 Publications

Catalytic activityi

ATP + H2O + monosaccharide(Out) = ADP + phosphate + monosaccharide(In).UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 538ATPUniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • galactose transmembrane transporter activity Source: EcoCyc
  • monosaccharide-transporting ATPase activity Source: UniProtKB-EC

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • galactose transport Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sugar transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:MGLA-MONOMER.
ECOL316407:JW2136-MONOMER.
MetaCyc:MGLA-MONOMER.

Protein family/group databases

TCDBi3.A.1.2.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactose/methyl galactoside import ATP-binding protein MglAUniRule annotation (EC:3.6.3.17UniRule annotation)
Gene namesi
Name:mglAUniRule annotation
Ordered Locus Names:b2149, JW2136
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10592. mglA.

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Galactose/methyl galactoside import ATP-binding protein MglAPRO_0000092512Add
BLAST

Proteomic databases

EPDiP0AAG8.
PaxDbiP0AAG8.
PRIDEiP0AAG8.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MglA), two transmembrane proteins (MglC) and a solute-binding protein (MglB).UniRule annotation

Protein-protein interaction databases

BioGridi4260868. 6 interactions.
IntActiP0AAG8. 13 interactions.
STRINGi511145.b2149.

Structurei

3D structure databases

ProteinModelPortaliP0AAG8.
SMRiP0AAG8. Positions 13-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 249236ABC transporter 1UniRule annotationAdd
BLAST
Domaini264 – 506243ABC transporter 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Galactose/methyl galactoside importer (TC 3.A.1.2.3) family. [View classification]UniRule annotation
Contains 2 ABC transporter domains.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105C2J. Bacteria.
COG1129. LUCA.
InParanoidiP0AAG8.
KOiK10542.
OMAiKWVIDSM.
PhylomeDBiP0AAG8.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015862. ABC_transpr_MglA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS51260. MGLA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSSTTPSSG EYLLEMSGIN KSFPGVKALD NVNLKVRPHS IHALMGENGA
60 70 80 90 100
GKSTLLKCLF GIYQKDSGTI LFQGKEIDFH SAKEALENGI SMVHQELNLV
110 120 130 140 150
LQRSVMDNMW LGRYPTKGMF VDQDKMYRET KAIFDELDID IDPRARVGTL
160 170 180 190 200
SVSQMQMIEI AKAFSYNAKI VIMDEPTSSL TEKEVNHLFT IIRKLKERGC
210 220 230 240 250
GIVYISHKME EIFQLCDEVT VLRDGQWIAT EPLAGLTMDK IIAMMVGRSL
260 270 280 290 300
NQRFPDKENK PGEVILEVRN LTSLRQPSIR DVSFDLHKGE ILGIAGLVGA
310 320 330 340 350
KRTDIVETLF GIREKSAGTI TLHGKQINNH NANEAINHGF ALVTEERRST
360 370 380 390 400
GIYAYLDIGF NSLISNIRNY KNKVGLLDNS RMKSDTQWVI DSMRVKTPGH
410 420 430 440 450
RTQIGSLSGG NQQKVIIGRW LLTQPEILML DEPTRGIDVG AKFEIYQLIA
460 470 480 490 500
ELAKKGKGII IISSEMPELL GITDRILVMS NGLVSGIVDT KTTTQNEILR

LASLHL
Length:506
Mass (Da):56,415
Last modified:October 11, 2005 - v1
Checksum:i15F794D57E386BCF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 6913KCLFG…KDSGT → NACLVFIKRLRH (PubMed:3302609).CuratedAdd
BLAST
Sequence conflicti163 – 1631A → G in AAA24170 (PubMed:9278503).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59444 Genomic DNA. Translation: AAA24170.1.
U00096 Genomic DNA. Translation: AAC75210.1.
AP009048 Genomic DNA. Translation: BAE76626.1.
X05646 Genomic DNA. Translation: CAA29133.1.
PIRiD64983.
RefSeqiNP_416654.1. NC_000913.3.
WP_000255039.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75210; AAC75210; b2149.
BAE76626; BAE76626; BAE76626.
GeneIDi949036.
KEGGiecj:JW2136.
eco:b2149.
PATRICi32119643. VBIEscCol129921_2232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59444 Genomic DNA. Translation: AAA24170.1.
U00096 Genomic DNA. Translation: AAC75210.1.
AP009048 Genomic DNA. Translation: BAE76626.1.
X05646 Genomic DNA. Translation: CAA29133.1.
PIRiD64983.
RefSeqiNP_416654.1. NC_000913.3.
WP_000255039.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAG8.
SMRiP0AAG8. Positions 13-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260868. 6 interactions.
IntActiP0AAG8. 13 interactions.
STRINGi511145.b2149.

Protein family/group databases

TCDBi3.A.1.2.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

EPDiP0AAG8.
PaxDbiP0AAG8.
PRIDEiP0AAG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75210; AAC75210; b2149.
BAE76626; BAE76626; BAE76626.
GeneIDi949036.
KEGGiecj:JW2136.
eco:b2149.
PATRICi32119643. VBIEscCol129921_2232.

Organism-specific databases

EchoBASEiEB0587.
EcoGeneiEG10592. mglA.

Phylogenomic databases

eggNOGiENOG4105C2J. Bacteria.
COG1129. LUCA.
InParanoidiP0AAG8.
KOiK10542.
OMAiKWVIDSM.
PhylomeDBiP0AAG8.

Enzyme and pathway databases

BioCyciEcoCyc:MGLA-MONOMER.
ECOL316407:JW2136-MONOMER.
MetaCyc:MGLA-MONOMER.

Miscellaneous databases

PROiP0AAG8.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR015862. ABC_transpr_MglA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS51260. MGLA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGLA_ECOLI
AccessioniPrimary (citable) accession number: P0AAG8
Secondary accession number(s): P23199, P76442, Q2MAT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.