Reviewed, UniProtKB/Swiss-Prot P0AAG7 (MDLB_ECO57)

Names and origin

Protein names

Recommended name:
    Multidrug resistance-like ATP-binding protein mdlB
    EC=3.6.3.44

Gene names

Name:	mdlB
Ordered Locus Names:	Z0559, ECs0503

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Drug exporter-2 (TC 3.A.1.117) family. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Multidrug resistance-like ATP-binding protein mdlB		PRO_0000092497
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 46	21	Potential
	Topological domain	47 – 62	16	Periplasmic Potential
	Transmembrane	63 – 83	21	Potential
	Topological domain	84 – 140	57	Cytoplasmic Potential
	Transmembrane	141 – 161	21	Potential
	Topological domain	162 – 164	3	Periplasmic Potential
	Transmembrane	165 – 185	21	Potential
	Topological domain	186 – 254	69	Cytoplasmic Potential
	Transmembrane	255 – 275	21	Potential
	Topological domain	276 – 278	3	Periplasmic Potential
	Transmembrane	279 – 299	21	Potential
	Topological domain	300 – 593	294	Cytoplasmic Potential
	Domain	25 – 310	286	ABC transmembrane type-1
	Domain	341 – 574	234	ABC transporter
	Nucleotide binding	374 – 381	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P0AAG7-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: CF1EE70009EB05D8	FASTA	593	65,222
	10 20 30 40 50 60 MRSFSQLWPT LKRLLAYGSP WRKPLGIAVL MMWVAAAAEV SGPLLISYFI DNMVAKNNLP 70 80 90 100 110 120 LKVVAGLAAA YVGLQLFAAG LHYAQSLLFN RAAVGVVQQL RTDVMDAALR QPLSEFDTQP 130 140 150 160 170 180 VGQVISRVTN DTEVIRDLYV TVVATVLRSA ALVGAMLVAM FSLDWRMALV AIMIFPVVLV 190 200 210 220 230 240 VMVIYQRYST PIVRRVRAYL ADINDGFNEI INGMSVIQQF RQQARFGERM GEASRSHYMA 250 260 270 280 290 300 RMQTLRLDGF LLRPLLSLFS SLILCGLLML FGFSASGTIE VGVLYAFISY LGRLNEPLIE 310 320 330 340 350 360 LTTQQAMLQQ AVVAGERVFE LMDGPRQQYG NDDRPLQSGT IEVDNVSFAY RDDNLVLKNI 370 380 390 400 410 420 NLSVPSRNFV ALVGHTGSGK STLASLLMGY YPLTEGEIRL DGRPLSSLSH SALRQGVAMV 430 440 450 460 470 480 QQDPVVLADT FLANVTLGRD ISEERVWQAL ETVQLAELAR SMSDGIYTPL GEQGNNLSVG 490 500 510 520 530 540 QKQLLALARV LVETPQILIL DEATASIDSG TEQAIQHALA AVREHTTLVV IAHRLSTIVD 550 560 570 580 590 ADTILVLHRG QAVEQGTHQQ LLAAQGRYWQ MYQLQLAGEE LAASVREEES LSA

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG54799.1. BA000007 Genomic DNA. Translation: BAB33926.1.
PIR	C85542. G90691.
RefSeq	NP_286191.1. NP_308530.1.
3D structure databases
SMR	P0AAG7. Positions 11-575, 341-577.
ModBase	Search...
Genome annotation databases
GeneID	914605. 957421.
GenomeReviews	Gene locus Z0559 in contig AE005174_GR. Gene locus ECs0503 in contig BA000007_GR.
KEGG	ece:Z0559. ecs:ECs0503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758042.
OMA	VHEESGP.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0503-MONOMER.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDLB_ECO57

Accession

Primary (citable) accession number: P0AAG7
Secondary accession number(s): P30751, P75706, P77117

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)