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Protein

Multidrug resistance-like ATP-binding protein MdlB

Gene

mdlB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi374 – 3818ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:MDLB-MONOMER.
ECOL316407:JW5061-MONOMER.

Protein family/group databases

TCDBi3.A.1.106.13. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance-like ATP-binding protein MdlB (EC:3.6.3.44)
Gene namesi
Name:mdlB
Synonyms:mdl
Ordered Locus Names:b0449, JW5061
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14374. mdlB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence analysisAdd
BLAST
Transmembranei26 – 4621HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini47 – 6216PeriplasmicSequence analysisAdd
BLAST
Transmembranei63 – 8321HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini84 – 14057CytoplasmicSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini162 – 1643PeriplasmicSequence analysis
Transmembranei165 – 18521HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini186 – 25469CytoplasmicSequence analysisAdd
BLAST
Transmembranei255 – 27521HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini276 – 2783PeriplasmicSequence analysis
Transmembranei279 – 29921HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini300 – 593294CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Multidrug resistance-like ATP-binding protein MdlBPRO_0000092496Add
BLAST

Proteomic databases

PaxDbiP0AAG5.
PRIDEiP0AAG5.

Interactioni

Protein-protein interaction databases

BioGridi4261405. 173 interactions.
STRINGi511145.b0449.

Structurei

3D structure databases

ProteinModelPortaliP0AAG5.
SMRiP0AAG5. Positions 23-575.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 310286ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini341 – 574234ABC transporterPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
InParanoidiP0AAG5.
KOiK18890.
OMAiIVMIIYQ.
PhylomeDBiP0AAG5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AAG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSFSQLWPT LKRLLAYGSP WRKPLGIAVL MMWVAAAAEV SGPLLISYFI
60 70 80 90 100
DNMVAKNNLP LKVVAGLAAA YVGLQLFAAG LHYAQSLLFN RAAVGVVQQL
110 120 130 140 150
RTDVMDAALR QPLSEFDTQP VGQVISRVTN DTEVIRDLYV TVVATVLRSA
160 170 180 190 200
ALVGAMLVAM FSLDWRMALV AIMIFPVVLV VMVIYQRYST PIVRRVRAYL
210 220 230 240 250
ADINDGFNEI INGMSVIQQF RQQARFGERM GEASRSHYMA RMQTLRLDGF
260 270 280 290 300
LLRPLLSLFS SLILCGLLML FGFSASGTIE VGVLYAFISY LGRLNEPLIE
310 320 330 340 350
LTTQQAMLQQ AVVAGERVFE LMDGPRQQYG NDDRPLQSGT IEVDNVSFAY
360 370 380 390 400
RDDNLVLKNI NLSVPSRNFV ALVGHTGSGK STLASLLMGY YPLTEGEIRL
410 420 430 440 450
DGRPLSSLSH SALRQGVAMV QQDPVVLADT FLANVTLGRD ISEERVWQAL
460 470 480 490 500
ETVQLAELAR SMSDGIYTPL GEQGNNLSVG QKQLLALARV LVETPQILIL
510 520 530 540 550
DEATASIDSG TEQAIQHALA AVREHTTLVV IAHRLSTIVD ADTILVLHRG
560 570 580 590
QAVEQGTHQQ LLAAQGRYWQ MYQLQLAGEE LAASVREEES LSA
Length:593
Mass (Da):65,222
Last modified:October 11, 2005 - v1
Checksum:iCF1EE70009EB05D8
GO

Sequence cautioni

The sequence AAB40205 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAC36870 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti208 – 2092NE → KQ in AAC36870 (PubMed:7904973).Curated
Sequence conflicti336 – 3372LQ → FT in AAC36870 (PubMed:7904973).Curated
Sequence conflicti358 – 3581K → N in AAC36870 (PubMed:7904973).Curated
Sequence conflicti411 – 4144SALR → TRG in AAC36870 (PubMed:7904973).Curated
Sequence conflicti457 – 4582EL → DV in AAC36870 (PubMed:7904973).Curated
Sequence conflicti542 – 5421D → A in AAC36870 (PubMed:7904973).Curated
Sequence conflicti563 – 59331AAQGR…ESLSA → RPRDVLADVSTATCGRRAGS QRA in AAC36870 (PubMed:7904973).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08627 Unassigned DNA. Translation: AAC36870.1. Different initiation.
U82664 Genomic DNA. Translation: AAB40205.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73552.1.
AP009048 Genomic DNA. Translation: BAE76229.1.
PIRiA64775.
RefSeqiNP_414983.1. NC_000913.3.
WP_001256201.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73552; AAC73552; b0449.
BAE76229; BAE76229; BAE76229.
GeneIDi945088.
KEGGiecj:JW5061.
eco:b0449.
PATRICi32116051. VBIEscCol129921_0467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08627 Unassigned DNA. Translation: AAC36870.1. Different initiation.
U82664 Genomic DNA. Translation: AAB40205.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73552.1.
AP009048 Genomic DNA. Translation: BAE76229.1.
PIRiA64775.
RefSeqiNP_414983.1. NC_000913.3.
WP_001256201.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AAG5.
SMRiP0AAG5. Positions 23-575.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261405. 173 interactions.
STRINGi511145.b0449.

Protein family/group databases

TCDBi3.A.1.106.13. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP0AAG5.
PRIDEiP0AAG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73552; AAC73552; b0449.
BAE76229; BAE76229; BAE76229.
GeneIDi945088.
KEGGiecj:JW5061.
eco:b0449.
PATRICi32116051. VBIEscCol129921_0467.

Organism-specific databases

EchoBASEiEB4117.
EcoGeneiEG14374. mdlB.

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
InParanoidiP0AAG5.
KOiK18890.
OMAiIVMIIYQ.
PhylomeDBiP0AAG5.

Enzyme and pathway databases

BioCyciEcoCyc:MDLB-MONOMER.
ECOL316407:JW5061-MONOMER.

Miscellaneous databases

PROiP0AAG5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDLB_ECOLI
AccessioniPrimary (citable) accession number: P0AAG5
Secondary accession number(s): P30751
, P75706, P77117, Q2MBX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally proposed to be fused with MdlA.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.