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P0AAG5 (MDLB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multidrug resistance-like ATP-binding protein MdlB
EC=3.6.3.44
Gene names
Name:mdlB
Synonyms:mdl
Ordered Locus Names:b0449, JW5061
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ABC transporter superfamily. Drug exporter-2 (TC 3.A.1.117) family. [View classification]

Contains 1 ABC transmembrane type-1 domain.

Contains 1 ABC transporter domain.

Caution

Was originally (Ref.1) proposed to be fused with MdlA.

Sequence caution

The sequence AAB40205.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAC36870.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 593593Multidrug resistance-like ATP-binding protein MdlB
PRO_0000092496

Regions

Topological domain1 – 2525Cytoplasmic Potential
Transmembrane26 – 4621Helical; Potential
Topological domain47 – 6216Periplasmic Potential
Transmembrane63 – 8321Helical; Potential
Topological domain84 – 14057Cytoplasmic Potential
Transmembrane141 – 16121Helical; Potential
Topological domain162 – 1643Periplasmic Potential
Transmembrane165 – 18521Helical; Potential
Topological domain186 – 25469Cytoplasmic Potential
Transmembrane255 – 27521Helical; Potential
Topological domain276 – 2783Periplasmic Potential
Transmembrane279 – 29921Helical; Potential
Topological domain300 – 593294Cytoplasmic Potential
Domain25 – 310286ABC transmembrane type-1
Domain341 – 574234ABC transporter
Nucleotide binding374 – 3818ATP Potential

Experimental info

Sequence conflict208 – 2092NE → KQ in AAC36870. Ref.1
Sequence conflict336 – 3372LQ → FT in AAC36870. Ref.1
Sequence conflict3581K → N in AAC36870. Ref.1
Sequence conflict411 – 4144SALR → TRG in AAC36870. Ref.1
Sequence conflict457 – 4582EL → DV in AAC36870. Ref.1
Sequence conflict5421D → A in AAC36870. Ref.1
Sequence conflict563 – 59331AAQGR…ESLSA → RPRDVLADVSTATCGRRAGS QRA in AAC36870. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0AAG5 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: CF1EE70009EB05D8

FASTA59365,222
        10         20         30         40         50         60 
MRSFSQLWPT LKRLLAYGSP WRKPLGIAVL MMWVAAAAEV SGPLLISYFI DNMVAKNNLP 

        70         80         90        100        110        120 
LKVVAGLAAA YVGLQLFAAG LHYAQSLLFN RAAVGVVQQL RTDVMDAALR QPLSEFDTQP 

       130        140        150        160        170        180 
VGQVISRVTN DTEVIRDLYV TVVATVLRSA ALVGAMLVAM FSLDWRMALV AIMIFPVVLV 

       190        200        210        220        230        240 
VMVIYQRYST PIVRRVRAYL ADINDGFNEI INGMSVIQQF RQQARFGERM GEASRSHYMA 

       250        260        270        280        290        300 
RMQTLRLDGF LLRPLLSLFS SLILCGLLML FGFSASGTIE VGVLYAFISY LGRLNEPLIE 

       310        320        330        340        350        360 
LTTQQAMLQQ AVVAGERVFE LMDGPRQQYG NDDRPLQSGT IEVDNVSFAY RDDNLVLKNI 

       370        380        390        400        410        420 
NLSVPSRNFV ALVGHTGSGK STLASLLMGY YPLTEGEIRL DGRPLSSLSH SALRQGVAMV 

       430        440        450        460        470        480 
QQDPVVLADT FLANVTLGRD ISEERVWQAL ETVQLAELAR SMSDGIYTPL GEQGNNLSVG 

       490        500        510        520        530        540 
QKQLLALARV LVETPQILIL DEATASIDSG TEQAIQHALA AVREHTTLVV IAHRLSTIVD 

       550        560        570        580        590 
ADTILVLHRG QAVEQGTHQQ LLAAQGRYWQ MYQLQLAGEE LAASVREEES LSA

« Hide

References

« Hide 'large scale' references
[1]"Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance."
Allikmets R., Gerrard B.C., Court D., Dean M.C.
Gene 136:231-236(1993) [PubMed: 7904973] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: TAP90 / ATCC 47037.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L08627 Unassigned DNA. Translation: AAC36870.1. Different initiation.
U82664 Genomic DNA. Translation: AAB40205.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73552.1.
AP009048 Genomic DNA. Translation: BAE76229.1.
PIRA64775.
RefSeqNP_414983.1. NC_000913.2.

3D structure databases

ProteinModelPortalP0AAG5.
SMRP0AAG5. Positions 11-577.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000003733; EBESCP00000003733; EBESCG00000003052.
EBESCT00000014652; EBESCP00000013943; EBESCG00000013713.
GeneID945088.
GenomeReviewsGene locus JW5061 in contig AP009048_GR.
Gene locus b0449 in contig U00096_GR.
KEGGecj:JW5061.
eco:b0449.
PATRIC32116051. VBIEscCol129921_0467.

Organism-specific databases

EchoBASEEB4117.
EcoGeneEG14374. mdlB.

Phylogenomic databases

eggNOGCOG1132.
GeneTreeEBGT00050000008240.
HOGENOMHBG758042.
OMANDDYPLQ.
PhylomeDBP0AAG5.
ProtClustDBPRK10790.

Enzyme and pathway databases

BioCycEcoCyc:MDLB-MONOMER.

Gene expression databases

GenevestigatorP0AAG5.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
KOK06148.
PfamPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF90123. ABC_TM_1. 1 hit.
PROSITEPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDLB_ECOLI
AccessionPrimary (citable) accession number: P0AAG5
Secondary accession number(s): P30751 expand/collapse secondary AC list , P75706, P77117, Q2MBX7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: October 11, 2005
Last modified: January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents